The WellConnected
Cell
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Classes of cell-cell junctions
type
function
tight junctions
sealing
anchoring junctions
structural
interconnection
communicating
junctions
communication
ZO-1
ZO-3
DNA
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Sealers
Organizers
Claudins
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ß-catenin
Occludins
JAMs
Informants
PTEN
Tight (Occluding) Junctions
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dense tracer
Tight
Junctions
seal
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Tight Junction
interactome
Anchoring junctions strengthen
Anchoring junction
I. Belt Junction
Cell side
connection
Other side
connection
MF cyt oskelet on MF cyt oskelet on
Cell Adhesion
Molecule (CAM)
Cadherins
(Ca++ dependent)
II. Desmosome
IF and MF
cyt oskelet on
IF and MF
cyt oskelet on
desmogleins and
desmocollins
III. Hemidesmosome
IF cyt oskelet on
via plectin
(IFBP )
extracellular
matrix (ECM)
integrins
IV. Focal Adhesions
MF cyt oskelet on
via alpha actinin
and vinculin
(ABP s)
extracellular
matrix (ECM)
integrins
I. Belt (Adherens)
Junctions
in cardiac muscle
Cadherin CAMs
Belt Junctions back up tight junctions
The Adherens Junction interactome
The Adherens Junction Interactome
Forging links
GFP::actin
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II. Desmosomal
Junctions
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(Plakoglobins anchor MFs)
Cytokeratin
Desmosomes
A day in the life of an epithelium
Desmocollin
-GFP
Imaged over
a 24 hour
period
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III. Hemidesmosomes
anchor IMF to ECM
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RGD (Arg-Gly-Asp)
via integrin
Heterodimers
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(18 alpha x 8 beta)
ECM
Loss of hemidesmosomal connections
creates blistering diseases
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IV. Focal Adhesion sites (FAds)
link microfilaments to ECM
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FAd interactome
Focal Adhesion Kinase (FAK)
/VASp
FAds
anchor
stress
fibers
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Actin-GFP
and Vinculin
GFP::VASp localizes at FAds
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Special Delivery?
Microtubules target FAds
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GFP::tubulin
and
Ds-Red::zyxin (@ FAds)
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GFP::CLIP-170 (+TIP)
and
Ds-Red::zyxin (@ FAds)
Communicating Junctions
GFP-Connexin-43 localizes at cell-cell contact points
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Connexin hexamers
form gap junctions
2nm
fields
Gap junction fields
Tightlypacked gap
junctions
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Gap junctions permit low MW molecules
(<1kDa) to move between cells
scrape
Gap junction interactome
AFM of gap junction field reveals the 6fold symmetry of the connexion channel
+Ca++
-Ca++
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And a role for calcium in conductance regulation
Calcium Wave
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Electrical coupling in engineered heart
muscle tissue
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Plasmodesmata
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The symplast refers to the linked protoplasmic network in plants
Plant cell lineages are
linked in distinct
symplastic routes
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