Veterinary Entomology 208 Spring 2010 Magnification • Always start at lowest magnification (lowest power objective) • Move to higher objective as necessary (3-4 stations only) Focus • Adjust width of oculars • Adjust coarse, then fine focus • If on the highest magnification, only adjust the fine focus to avoid breaking slide Light • Use condenser to adjust amount of light (dark vs. glare) • Higher magnifications may require more light Be sure you read where the key is sending you next, as it can get confusing (particularly with lice) Look at arrow on insect to see what characteristic is being studied • The small arrows in the key indicate the structure referenced in the couplet Fleas (Siphonaptera) 6 specimens: 1. Ctenocephalides felis 2. Leptopsylla segnis 3. Nosopsyllus fasciatus 4. Echidnophaga gallinacea 5. Pulex irritans 6. Xenopysylla cheopis receptaculum seminis In front of eye Below eye Key 1st couplet: number of combs • We don’t have a 3 comb flea 2 comb fleas: • Eye presence/absence will separate these 2 species 1 comb flea: • There is only one in our set 0 comb fleas: • Head shape separates out Echidnophaga gallinacea • Ocular bristle position separates the other 2 species 12 specimens of Mallophaga: 1. 2. 3. 4. 5. 6. 7. 8. Heterodoxus spiniger Menacanthus stramineus Menopon gallinae Chelopistes meleagridis Goniodes gigas Goniocotes gallinae Goniodes dissimilis Lipeurus caponis Felicola subrostrata 10. Bovicola crassipes 11. Bovicola bovis 12. Bovicola limbata 9. 10 specimens of Anoplura: 1. Pediculus humanus 2. Phthirus pubis 3. Haematopinus suis 4. Haematopinus asini 5. Haematopinus eurysternus 6. Solenopotes caplillatus 7. 8. 9. 10. Linognathus africanus Linognathus setosus Linognathus pedalis Linognathus vituli