LEVADEX™, a Novel Orally Inhaled Treatment for Acute Migraine: Efficacy and Tolerability Results of a Phase 3 Study Shashidhar H Kori, MD Vice President, Clinical Development and Medical Affairs, MAP Pharmaceuticals Authors and Disclosures • Stephen D Silberstein1 ,MD • Advanced NeuroModulation Systems, AGA, Allergan, Boston Scientific, Capnia, Coherex, Endo, GSK, Lilly, MAP, Medtronic, Merck, NuPathe, Valeant • Shashidhar H Kori2 ,MD • MAP Pharmaceuticals • Sheena K Aurora3 ,MD • Advanced Bionics, Alexza, Allergan, Capnia, GSK, Kowa, MAP, Merck, OrthoMcNeil, Neuralieve, Nupathe, Takeda • Stewart J Tepper4 ,MD • ATI, GSK, MAP, Merck, St. Jude, Valeant • Scott W Borland2 ,MS • MAP Pharmaceuticals • Min H Wang2 ,PhD • MAP Pharmaceuticals • Albert E Reppine2 ,RN • MAP Pharmaceuticals • Thomas A Armer2 ,PhD • MAP Pharmaceuticals • David W Dodick5 ,MD • Allergan, ANS, Coherex, Endo, HS Lundbeck, Kowa, MAP, Medtronic, Merck, Minster, Neuralieve, Neuraxon, NuPathe, Pfizer, Wiley-Blackwell 1. Jefferson Medical College of Thomas Jefferson University, Neurology, Philadelphia, PA 2. MAP Pharmaceuticals, Mountain View, CA 3. Swedish Headache Center, Neurology, Seattle, WA 4. Cleveland Clinic, Center for Headache and Pain, Neurological Institute, Cleveland, OH 5. Mayo Clinic, Neurology, Scottsdale, AZ 2 Disclaimer LEVADEX™ (MAP0004) is an investigational product candidate and is not yet approved by the US Food and Drug Administration 3 3 Limitations of Existing Acute Migraine Therapies Slow onset of action Inconsistent response attack to attack • Inconsistent absorption through nasal mucosa • Inconsistent absorption of tablets due to gastric stasis 30% to 40% of patients nonresponsive to treatment High recurrence rate Limited efficacy when taken late in migraine May lead to medication overuse headache Poor tolerance (eg, triptan sensations, nausea) 4 4 Dihydroergotamine: Well Established Efficacy and Its Role in Treatment of Migraine Advantages Proven efficacy in the treatment of migraine for more than 60 years Gold standard in refractory migraine Commonly used drug for medication overuse headache (MOH) 5 5 Dihydroergotamine: Limitations Due to Formulation Disadvantages Poor oral bioavailability Inconvenient to administer IV Nausea associated with injection therapy Inconsistent absorption when administered through routes other than IV 6 6 Proprietary TEMPO® Inhaler Optimizes Pulmonary Delivery Breath actuated Plume velocity reduced Consistent delivery of drug Minimal oropharyngeal deposition Expected to deliver most drug deep into the lung 7 7 TEMPO® Inhaler Enables Efficient and Consistent Delivery to the Lung Radiolabeled fluticasone propionate canister in standard inhaler Radiolabeled fluticasone propionate canister in TEMPO® Inhaler TEMPO inhaler provides improved delivery vs commercial metered dose inhaler • • • 3-fold more drug in the lungs 4-fold less drug in the mouth and throat 2-fold reduction in dose-to-dose variability Shrewsbury SB, et al. Int J Pharm. 2008;356:137-143. 8 8 Phase 1 PK: LEVADEX is Associated with Significantly Lower Cmax than DHE Delivered Via Intravenous Infusion Significant IV plasma “spike” ↑ Potential for side effects LEVADEX LEVADEX LEVADEX LEVADEX Suppressed inhaled plasma Cmax ↓ Potential for side effects Shrewsbury SB, et al. Headache. 2008; 48:355-367. 9 MAP0004 Tmax Compared with Non-IV Routes of Administration DHE Tmax Based on Delivery 1 1 2 2 1 3 4 4 MAP0004 4 1Van der Kuy. P-HM, et al . Eur J Clin Pharmacol. 1999;55:677-68. H, et al. Clin Pharmacol Ther. 1996;60:265-275. 3de Hoon JN, et al. Br J Clin Pharmacol. 2001;52:45-51. *Nominal dose. 4Shrewsbury SB, et al. Headache. 2008;48:355-367. IM = intramuscular; SQ = subcutaneous; IV = intravenous. 10 10 10 2Humbert FREEDOM-301 Objectives, Design, Endpoints Objectives: To evaluate the efficacy and safety of LEVADEX (previously MAP0004), a novel orally inhaled form of dihydroergotamine (DHE), in the treatment of acute migraine compared to placebo FREEDOM-301 Study Design Endpoints Co-Primary: • Pain relief at 2 hrs • Phonophobia free at 2 hrs • Photophobia free at 2 hrs • Nausea free at 2 hrs Treatment Period 1 Double-Blind (Up to 8 weeks) Run In (At least 28 days) Active Treatment Periods 2 and 3 Open Label Safety Placebo Visit 1 Visit 2 Visit 3 Secondary • Sustained pain relief 2-24 hrs • Time to pain relief • Pain relief at 4 hours • Pain relief at 10 minutes 11 Phase 3: Baseline Demographics Parameter Age, years Mean (SD) Median (range) Sex, n (%) Female Male HIT-6 Score Mean (SD) Median (range) Disability due to headache, n (%) ≥ 60 HIT-6 score < 60 HIT-6 score Missing LEVADEX 1.0 mg (n = 395) Placebo (n = 397) 40.5 (11) 42 (18 – 65) 39.6 (12) 40 (18 – 65) 363 (92) 32 (8) 362 (91) 35 (9) 65.5 (4.9) 66 (50 – 78) 65.6 (5.0) 65 (48 – 78) 352 (89) 41 (10) 2 (1) 357 (90) 36 (9) 4 (1) 12 12 Phase 3: At the Time of Treatment, Intensity of Migraine Symptoms Were Similar in Both Treatment Groups = Mild = Moderate = Severe = Allodynia * Nausea Phonophobia Photophobia Allodynia *At time of treatment, nausea scores were missing for 2 patients who received LEVADEX. 13 13 Phase 3: All Co-Primary Endpoints Met Parameter LEVADEX 1.0 mg (n = 395) Placebo (n = 397) P value* Pain relief at 2 hours, n (%) 232 (59%) 137 (35%) < .0001 Phonophobia free at 2 hours, n (%) 209 (53%) 134 (34%) < .0001 Photophobia free at 2 hours, n (%) 184 (47%) 108 (27%) < .0001 Nausea free at 2 hours, n (%) 265 (67%) 233 (59%) .02 *P value vs placebo calculated using the Cochran-Mantel-Haenszel test controlling for baseline pain score, adjusted for multiplicity 14 14 Phase 3: Secondary Endpoints Demonstrated Rapid Pain Relief that is Sustained at 24 Hours with LEVADEX Parameter LEVADEX 1.0 mg Placebo P value* Sustained Pain Relief from 2-24 Hrs, n (%) 167 (44%) n = 382 76 (20%) n = 387 < .0001 30 n = 384 — n = 381 .0266† 254 (65%) n = 393 144 (37%) n = 391 < .0001 33 (9%) n = 388 22 (6%) n = 387 .1584 Time to pain relief, minutes Pain Relief at 4 Hours, n (%) Pain Relief at 10 Minutes, n (%) *P value vs placebo calculated using the Cochran-Mantel-Haenszel test controlling for baseline pain score, adjusted for multiplicity. †P value vs placebo calculated using the 2-sided log-rank test. 15 15 Pain Relief Pain Relief Sustained Pain Relief Proportion of Subjects with Pain Relief p<0.0001 70% p<0.0001 59% 60% 50% 65% Time to pain relief was 30 minutes (p=0.0266) 40% * 44% + 29% 30% p<0.0001 48% 35% * 37% 36% 28% 22% 20% 20% 17% p=0.1584 10% Placebo LEVADEX 9% 6% 0% 10min 30min 60min 2hrs 4hrs Primary and secondary endpoints marked in red + p=0.0245, * p<0.0001 Note: p-values from exploratory endpoints not adjusted for multiplicity 2-24hrs 2-48hrs 16 Pain Free Pain Free Sustained Pain Free 45% Proportion of Subjects Pain Free * 39% 40% 35% * 28% 30% * 25% 23% * 20% * 18% 17% 15% Placebo LEVADEX 15% 10% + 10% 5% 5% 0.5%1% 7% 5% 6% 1% 0% 10min 30min 60min 2hrs 4hrs All exploratory endpoints + p=0.0024, * p<0.0001 Note: p-values from exploratory endpoints not adjusted for multiplicity 2-24hrs 2-48hrs 17 Proportion of Patients at 2 Hours Phonophobia, Photophobia, Nausea Free 80% p=0.0210 70% 67% p<0.0001 60% p<0.0001 53% 47% 50% 40% 59% Placebo LEVADEX 34% 27% 30% 20% 10% 0% Phonophobia Free Photophobia Free Nausea Free Primary and secondary endpoints marked in red 18 Phase 3: LEVADEX is Generally Well Tolerated in Adults with Migraine Adverse Events in ≥ 2% of Patients and Greater Than Placebo Patients, n (%) LEVADEX (N = 404) Placebo (N = 401) Pharma product complaint (taste) 26 (6.4) 7 (1.7) Nausea 18 (4.5) 8 (2.0) Cough 10 (2.5) 5 (1.2) Parameter ● In patients treated with LEVADEX, symptoms and sensitivities typically associated with triptans, such as chest discomfort (1%) and chest pain (0%) were rare and occurred at similar rates compared with placebo ● No serious adverse events deemed related to study drug were reported 19 19 Adverse Event Profile Double-Blind Period Run-In Period (N=805) Placebo (N=401) LEVADEX (N=404) 140 (17.4%) 101 (25.2%) 126 (31.2%) Upper Respiratory Tract Infection 22 (2.7%) 12 (3.0%) 12 (3.0%) Nasopharyngitis 14 (1.7%) 14 (3.5%) 8 (2.0%) Sinusitis 16 (2.0%) 5 (1.2%) 3 (0.7%) Nausea 1 (0.1%) 8 (2.0%) 18 (4.5%) Vomiting 1 (0.1%) 3 (0.7%) 8 (2.0%) 4 (0.5%) 5 (1.2%) 10 (2.5%) 0 (0.0%) 7 (1.7%) 26 (6.4%) Adverse Events 2% in Any Group Number (%) of Patients Reporting at Least 1 AE Infections and Infestations Gastrointestinal Disorders Respiratory, Thoracic and Mediastinal Disorders Cough Social Circumstances Pharma Product Complaint (Indication of Taste) • Symptoms and sensitivities typically associated with triptans, such as chest discomfort (1%), chest pain (0%) and paresthesia (0.5%), were rare and comparable to placebo • No decrease in pulmonary function (spirometry) compared to placebo group 20 Additional Analyses (Post Hoc): 2 hr pain relief Allodynia 70% 60% 50% % of 40% Patients 30% 20% 10% 0% Time to Treatment * p< 0.0001 70% * * * 60% + + 50% % of Patients 40% 30% 20% 10% 0% With Allodynia < 1hr Without Allodynia Placebo LEVADEX 1-4 hrs Placebo Morning Migraine 4-8 hrs >8 hrs LEVADEX Level of Migraine Disability + p<0.05, * p< 0.0001 70% 60% 50% % of 40% Patients 30% 20% 10% 0% * p< 0.0001, + p<0.05 * 70% * + + p<0.01, * p< 0.0001 * 60% 50% + % of Patients 40% 30% 20% 10 % 0% Morning Migraine Rest of Day HIT-6 <60 Placebo LEVADEX Placebo HIT-6 >= 60 LEVADEX 21 Additional Analyses (Post Hoc) Pain Severity Pain Relief 80 70 70% 60 60% 50 50% % of 40 Patients % of Patients 30 2 hr PainNausea Relief * p< 0.0001 * * 40% 30% 20% 20 10% 10 0% 0 10 Minutes 30 Minutes 60 Minutes LEVADEX Moderate LEVADEX Severe Placebo Moderate Placebo Severe Placebo Without Nausea LEVADEX Aura 2 hr Pain Relief 2 hr Pain Relief + p<0.05, * p< 0.0001 70% * 60% 50% With Nausea Vomiting 70% % of Patients 4 hrs 2 hrs 60% + p=0.0002, * p< 0.0001 + * 50% + % of Patients 40% 30% 40% 30% 20% 20% 10 % 10 % 0% 0% With Vom iting Without Vom iting Placebo LEVADEX With Aura Placebo Without Aura LEVADEX 22 Conclusions • In this trial, LEVADEX demonstrated rapid and sustained efficacy in treating acute migraine • LEVADEX was well tolerated with minimal side effects, and low incidence of triptan-like sensations • LEVADEX was effective in a broad spectrum of migraine, including those thought to be generally treatment resistant 23