Vorapaxar, a Platelet Thrombin Receptor Antagonist, in Acute Coronary Syndromes Kenneth W. Mahaffey, MD, on behalf of the TRACER Investigators and Committees Trial funded by Merck Complete financial disclosures: www.DCRI.org Background Platelet • Vorapaxar PAR-1 Thrombin • PAR-4 Clopidogrel Prasugrel Ticagrelor Cangrelor ADP TBX A2 ASA Anionic phospholipid surfaces P2Y12 TBXA2-R • Vorapaxar: First-in-class Oral PAR-1 inhibitor Metabolism: Primarily hepatic via CYP 3A4 Terminal half-life: ~126–269 hrs Prior trials: No increase in bleeding and fewer MIs GP IIb/IIIa Chackalamannil S, J Med Chem, 2006 Trial Design NSTE Acute Coronary Syndromes Key inclusion criteria • Within 24 hrs of symptoms • biomarkers or ECG changes • 1 other high-risk feature Placebo 1:1 Randomized Double-blind Vorapaxar Loading: 40 mg Maintenance: 2.5 mg daily Follow-up: 1, 4, 8, 12 months, then every 6 months Standard of care based on practice guidelines Efficacy Endpoints Primary: CV death, MI, stroke, hospitalization for ischemia, urgent revascularization Key Secondary: CV death, MI, stroke Bleeding Endpoints: GUSTO moderate or severe and clinically significant TIMI bleeding Statistical Considerations • • • Sample size Event-driven with estimated 15% reduction Power: • 1900 primary endpoint events >95% • 1457 key secondary endpoint events ≥90% 12,500 patients Analysis Efficacy analyses: intention-to-treat Bleeding analyses: all subjects with ≥1 dose and on drug Hierarchical testing procedure to control overall type 1 error January 8, 2011: DSMB recommended to stop follow-up in the trial Enrollment 37 countries, 818 sites, 12,944 patients Poland: 561 Norway: 251 Sweden: 346 Canada: 591 United States: 2772 Puerto Rico: 41 Colombia: 275 Peru: 11 Chile: 148 Denmark: 205 U.K.: 463 Netherlands: 471 Belgium: 153 Germany: 911 Portugal: 189 France: 441 Turkey: 164 Japan: 276 China: 219 South Korea: 127 Taiwan: 219 Spain: 379 Czech Rep: 496 Brazil: 284 Finland: 119 Hungary: 266 Switzerland: 211 Hong Kong: 17 Malaysia: 52 Singapore: 26 Austria: 319 Italy: 764 Argentina: 130 Israel: 410 South Africa: 207 Australia: 235 New Zealand: 195 Study Conduct Placebo (N=6471) Vorapaxar (N=6473) 30 (0.5) 27 (0.4) 1726 (27) 1818 (28) Treatment duration (days) 393 (236, 588) 379 (231, 585) Follow-up duration (days) 503 (348, 667) 500 (349, 668) 8 (0.1) 7 (0.1) Did not receive treatment (%) Discontinued treatment (%) Lost to follow-up (%) Median (IQR) Baseline Demographics Placebo (N=6471) Vorapaxar (N=6473) 64 (58, 72) 64 (58, 71) Female sex, % 28 28 Region of enrollment, % North America South America Western Europe Eastern Europe Asia Australia/New Zealand 26 7 45 12 7 3 26 7 45 12 7 3 Diabetes mellitus, % 31 32 Prior MI, % 29 29 Positive troponin or CK-MB, % 94 94 Antiplatelet agents, % Aspirin Thienopyridine 97 87 96 88 Age, yrs Median (IQR) Index Hospitalization Procedures Placebo (N=6471) Vorapaxar (N=6473) Hospital presentation to randomization (hrs) 21 (12, 41) 21 (12, 41) Symptom onset to randomization (hrs) 27 (8, 50) 27 (18, 49) 88 88 57 4 (2, 21) 58 46 58 4 (2, 21) 56 49 10 10 Cardiac catheterization, % PCI, % Loading dose of study drug to PCI (hrs) Drug-eluting stent, % Bare metal stent, % CABG, % Median (IQR) Primary Endpoint CV Death, MI, Stroke, Hospitalization for Ischemia, Urgent Revascularization 2-year KM rate Placebo Vorapaxar 19.9% 18.5% HR (95% CI): 0.92 (0.85, 1.01) P-value= 0.072 No. at risk Placebo Vorapaxar 6471 5844 6473 5897 5468 5570 5121 5199 3794 3881 2291 2318 795 832 Key Secondary Endpoint CV Death, MI, Stroke 2-year KM rate Placebo Vorapaxar 16.4% 14.7% HR (95% CI): 0.89 (0.81, 0.98) P-value= 0.018 No. at risk Placebo Vorapaxar 6471 5895 6473 5949 5575 5684 5263 5356 3922 4023 2383 2427 830 868 Selected Efficacy Outcomes Placebo (N=6471) Vorapaxar (N=6473) 2-yr 2-yr KM rate (%) KM rate (%) Primary endpoint HR (95% CI) P-value 19.9 18.5 0.92 (0.85–1.01) 0.072 CV death 3.8 3.8 1.00 (0.83–1.22) 0.96 MI 12.5 11.1 0.88 (0.79–0.98) 0.021 Stroke 2.1 1.9 0.93 (0.70–1.23) 0.61 Hospitalization for ischemia 1.5 1.6 1.14 (0.83–1.58) 0.42 Urgent revascularization 3.5 3.8 1.07 (0.88–1.31) 0.49 Stent Thrombosis* 1.5 1.7 1.12 (0.78–1.62) 0.54 All-cause mortality 6.1 6.5 1.05 (0.90–1.23) 0.52 *ARC definite or probable; data are proportions of patients Bleeding Endpoints Placebo (N=6441) Vorapaxar (N=6446) 2-yr KM rate (%) 2-yr KM rate (%) HR (95% CI) P-value GUSTO moderate or severe 5.2 7.2 1.35 (1.16–1.58) <0.001 Clinically significant TIMI 14.6 20.2 1.43 (1.31–1.57) <0.001 GUSTO severe 1.6 2.9 1.66 (1.27–2.16) <0.001 TIMI major 2.5 4.0 1.53 (1.24–1.90) <0.001 Fatal 0.15 0.35 1.89 (0.80–4.45) 0.15 Intracranial hemorrhage 0.24 1.07 3.39 (1.78–6.45) <0.001 CABG-related TIMI major* 7.3 9.7 1.34 (0.92–1.95) 0.13 * data are proportions of patients Bleeding Outcomes GUSTO Moderate/Severe Placebo Vorapaxar 5.2% 7.2% 2-year KM rate ICH 2-year KM rate Placebo Vorapaxar 0.24% 1.07% HR (95% CI): 3.39 (1.78, 6.45) P-value <0.001 HR (95% CI): 1.35 (1.16, 1.58) P-value <0.001 No. at risk 6441 5536 5137 4674 6446 5529 5108 4598 3393 3278 1972 1883 650 625 No. at risk 6441 5673 5281 4823 6446 5694 5272 4760 3511 3411 2038 1965 678 657 Subgroups GUSTO Moderate/Severe Vorapaxar better Placebo better Primary Endpoint Vorapaxar better Placebo better Summary When added to standard of care in patients with NSTE ACS and high use of aspirin and P2Y12 inhibition, vorapaxar: • Did not significantly reduce the composite of CV death, MI, stroke, hospitalization for ischemia, or urgent revascularization • Reduced CV death, MI, or stroke • Significantly increased bleeding, including major bleeding and intracranial hemorrhage Whether PAR-1 blockade improves outcomes with different medication strategies or in other patient populations with coronary artery disease requires further study. Study Organization Executive Committee Academic Research Organizations R Harrington (Chair), P Armstrong, P Aylward, E Chen, K Mahaffey, D Moliterno, J Strony, F Van de Werf, L Wallentin, H White DCRI: P Tricoci, T Rorick, S Leonardi, D Underwood, J Wrestler CVC: P Armstrong, C Sorochuck C5: A Lincoff, D Mason Henry Ford: M Hudson, D Sydlowski Thomas Jefferson: D Whellan, B Gallagher Flinders: P Aylward, J Garrett Green Lane: H White, S Douglas Leuven: P Sinnaeve, A Beernaert Steering Committee G Ambrosio, A Betriu, C Bode, A Cequier, T Cheem, M Chen, J Cornel, A Dalby, R Diaz, A Erkan, P Grande, C Held, K Huber, M Hudson, Y Huo, D Isaza, J Jukema, M Laine, B Lewis, A Lincoff, J Lixin, G Montalescot, J Nicolau, J Nordrehaug, P Ofner, H Ogawa, S Park, M Pfisterer, J Prieto, L Providencia, W Ruzyllo, P Sinnaeve, R Storey, P Tricoci, M Valgimigli, D Whellan, P Widimsky, L Wong, T Yamaguchi Sponsor Merck: E Chen, R Harmelin–Kadouri, A Kilian, S Petrauskas, J Strony Data & Safety Monitoring Board CEC Core Lab F Verheugt (Chair), R Frye, J Hochman, P Steg, K Bailey, J Easton A Johnson J O’ Briant M Smith P Tricoci ECG: P Armstrong, H Siha Platelets: L Jennings, E Hord Angio: M Gibson, A Chirlin The full article is now available online at www.nejm.org