ก ความหลากชนิ ดและการกระจายตัวของแมลงหนอนปลอกนา้ ้ ป่่ าอนุ ร ักษ ์ และอุทยานแห่งชาติของประเทศไทย ในพืนที Diversity and distribution of caddisflies in the protected areas and national parks of Thailand ดร.กิตย ิ า ถาวโรฤทธิ์ ภาควิชาวิทยาศาสตร์ชวี ภาพ คณะ วิทยาศาสตร์และเทคโนโลยี Introduction Holometabolous Trichoptera or Caddisflies *Important components of food web in freshwater ecosystem. *Used for freshwater biomonitoring. Caseless caddisflies Cased caddisflies adults http://images.google.co.th/images?hl=th&source=hp&q=trichoptera&oq=&um=1&ie=UTF-8&sa=N&tab=wi 2 Introduction (cont.) Microhabitat: Freshwater streams Waterfall Headwater stream 3 Studying caddisflies in Thailand Up to date, at least 1,000 caddisflies species had been reported in Thailand. Prommi (2007) reported 15 genera, 49 caddisflies species in Narathiwat Province. However, rare reported caddisflies species in Narathiwat ! Sirindorn Waterfall 4 Field sampling 5 Materials and methods Collecting Samples Larvae Hand Picking D-frame net Adults Ultraviolet light trap Sheet 6 Materials and methods (cont.) Water quality measurements Water temperature (°C) Air temperature (°C) Stream depth (cm) and width (m) Current velocity (m/s) Electrical conductivity (µs/cm) Total dissolved solids (mg/L) Dissolved oxygen: DO (mg/L) pH pH meter (HI 98130) DO meter (YSI 550A) 7 Identified to species level Specimens were preserved in absolute ethanol. Male genitalia were cleared in hot 10% KOH and identified to species level through a microscope using keys and an atlas. Stereomicroscope Inferior Appendages Segment X Segment IX Phallus 8 Keys for morphospecies Representative genera * Diplectrona Potamyia Cheumatopsyche Amphipsyche Hydromanicus Macrostemum Hydropsyche Scale= 0.5 cm *= 91 mm Representative species Macrostemum midas Pseudoleptonema quinquefasciatum Macrostemum floridum Macrostemum seba 10 Scale=0.5 cm Workshop and Training: EPT Course & Immature insects Course 11 International Conference 14th International Symposium on Trichoptera 12 Colleagues Assoc. Prof. Dr. Narumon Sangpradub Prof. Emeritus Dr. John C. Morse Khon Kaen University Clemson University, SC, USA. Dr. Xin Zhou BGI-Research, Shenzhen, China 13 Thank you for your kind attention ึ ษาผูช ่ ยวิจ ัย ร ับน ักศก ้ ว จานวนจาก ัดค่ะ 14 http://www.biology.science.cmu.ac.th/research/AIRU%20data%20online/AIRU_website/Trichoptera_general.htm