Part 2

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Biological Research in Canisters
BRIC-LED
Actuation
tool
interface
Illumination
controls
BRICLED
Canister
Petri Dish
Sealed Petri Dish
Fixation Unit
Liquid
reservoir
• Up to six 60mm x 15mm Petri
Dishes per BRIC canister
• Eight canisters per half locker
tray
• 3 sealed levels of containment
• Unidirectional LED illumination
• Single or dual stage manual
liquid (fixatives, growth
inhibitors, etc.) introduction
• Autonomous environmental
data logging
Enables Liquid Treatments (e.g. Cytochalasin) Followed by In-flight Fixation
ESC 00415—EAR 99
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Biological Research in Canisters
BRIC-Opti
Gas sampling port
Lid
OptiCell™
Culture
Chambers
Multichannel temperature
and humidity logger
Base
OptiCell™
Culture
Chambers
• 2 sealed levels of
containment
• Gas sampling capable
• Passive
• Can be frozen (-100 °C)
• Contains up to 4 OptiCell™
culture chambers
• Autonomous environmental
data logging
For Passive Tissue Culture/Microbial Growth in COTS Opticells
ESC 00415—EAR 99
2
Kennedy Space Center Fixation Tube
(KFT)
Main Tube
Actuator
Expansion Plug
Sample Tube
2.365” L x 0.875” D
• Provides three levels of containment during all
phases
• No glovebox required
• Samples may be transferred from other growth
facilities
• Maintains robust containment down to -100
°C
For Crew-Facilitated In-Flight Specimen Fixation
ESC 00415—EAR 99
Chemical Preservative:
•3% glutaraldehyde
•5% formaldehyde
•5% DMSO
•RNAlater™
•b-glucuronidase stain
•5% formalin
5% acetic acid
63% ethanol
•0.5% glutaraldehyde
2% paraformaldehyde
3
FASTRACK™ Suborbital Platform
• Provides compatibility across
the entire fleet of emerging
suborbital vehicles, existing
and future parabolic flight
providers, and future orbital
free-flyers or transports
• Supports 2 single middeck
locker equivalents or 1
double locker
• Provides power and data
acquisition
FASTRACK
Structural
View
Parabolic
Flight Testing
Provides a Standard Platform for Suborbital and Parabolic Investigations
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Payload Integration &
Operations
• 52 payloads have been managed and integrated
• Platforms: shuttle, ISS, SPACEHAB, Progress, MPLM,
Spacelab, Mir
• Joint foreign collaborations with CSA, DLR, ESA, JAXA,
NSAU
• Capabilities for hardware engineering, integration,
operations support, and project science coordination
• Experiment Monitoring Area (EMA) operations control
center at KSC for real time support
• ISS Environmental Simulator (ISSES) chambers provide
simulated spaceflight environment for analog ground
controls
Integration History and Capabilities
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Payload Development:
A Phased Approach
Pre-Phase A
Phase A
Phase B
Phase C
Phase D
Phase E
• Each phase includes a final milestone or gate
• Each phase has distinct activities associated with science, hardware, and
analytical integration
• Phase duration is a function of many factors
– Experiment maturity
– Hardware maturity
– Platform
Payload Lifecycle
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Payloads in the land of “OZ”
Payload Integration Manager Schedule
STRATEGIC
PDR
•
•
•
•
Safety
Phase 0/I
CDR
TACTICAL
Safety
Phase II
Export Classification Letter
Prelim Design Data Package
Safety Data (Phase 0-I)
Training Strategy TIMs
Payload
Pre-Ship
Safety
Phase III
CoFR
• Safety Data Pack
• Payload Integration
Agreement (PIA)
• Critical Design Data Package
• Safety Data (Phase II)
• ICD/Verification Requirements
• Resource Requirements Definition
• Procedures & Displays DS (P)
Key:
P – Preliminary
U – Update
F – Final
Payload
Manifested
•
•
•
•
•
•
•
•
C&DH DS (P)
KSC Support Rqmts DS
KSC Technical Rqmts DS
Ground Data Services DS
Procedures & Displays (U)
Payload Planning DS (P)
Manifest/Stowage DS (P)
Drawing DS (P)
•
•
•
•
• Manifest/Stowage DS (U)
• Drawing DS (U)
• Integration Data Pack (IDP)
• C&DH DS (F)
• Procedures & Displays (F)
C&DH DS (U)
Payload Trainer H/W & S/W
Training Plans & Courseware
Payload Planning DS (F)
• Interface Control Document (ICD) (U)
• Payload Verification Plan (PVP) (U)
• Requirements Exceptions
Why an Implementation Partner is Important
ESC 00415—EAR 99
• CoFR
Endorsement
7
Acronyms
ABRS
BRIC
BRIC-LED
COTS
CSA
DLR
DMSO
ECP
EMA
ERC
ESA
EXPRESS
GCU
GFP
ISS
ISSES
JAXA
KFT
KSC
LSSC
MPLM
NSAU
TOSC
Advanced Biological Research System
Biological Research in Canisters
Biological Research in Canisters – Light Emitting Diode
Commercial Off-the-Shelf
Canadian Space Agency
Deutsches Zentrum für Luft- und Raumfahrt
Dimethyl sulfoxide
Environmental Control Panel
Experiment Monitoring Area
Environmental Research Chamber
European Space Agency
Expediting the Processing of Experiments to the Space Station
Generic Containment Unit
Green Fluorescent Protein
International Space Station
ISS Environmental Simulator
Japan Aerospace Exploration Agency
Kennedy Space Center Fixation Tube
Kennedy Space Center
Life Science Services Contract
Mult-purpose Logistics Module
National Space Agency of Ukraine
Test and Operations Support Contract
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Points of Contact
Ground Research Support Point of Contact (ESC):
Michele N. Birmele
Advanced Life Support Laboratories Lead
Mail Code ESC-24
Kennedy Space Center, FL 32899
Phone 321.861.2940
Michele.N.Birmele@nasa.gov
Laboratory Support Point of Contact (TOSC):
Bruce E. Burch
TOSC Project Manager
Mail Code 721S-S100
Kennedy Space Center, FL 32899
Phone 321.867.5255
Bruce.E.Burch-1@nasa.gov
Hardware + Implementation Point of Contact (ESC):
David W. Reed
Payload Engineering Lead
Mail Code ESC-24
Kennedy Space Center, FL 32899
Phone 321.861.2980
David.W.Reed@nasa.gov
ESC 00415—EAR 99
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Thank you!
ESC 00415—EAR 99
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