Biological Research in Canisters BRIC-LED Actuation tool interface Illumination controls BRICLED Canister Petri Dish Sealed Petri Dish Fixation Unit Liquid reservoir • Up to six 60mm x 15mm Petri Dishes per BRIC canister • Eight canisters per half locker tray • 3 sealed levels of containment • Unidirectional LED illumination • Single or dual stage manual liquid (fixatives, growth inhibitors, etc.) introduction • Autonomous environmental data logging Enables Liquid Treatments (e.g. Cytochalasin) Followed by In-flight Fixation ESC 00415—EAR 99 1 Biological Research in Canisters BRIC-Opti Gas sampling port Lid OptiCell™ Culture Chambers Multichannel temperature and humidity logger Base OptiCell™ Culture Chambers • 2 sealed levels of containment • Gas sampling capable • Passive • Can be frozen (-100 °C) • Contains up to 4 OptiCell™ culture chambers • Autonomous environmental data logging For Passive Tissue Culture/Microbial Growth in COTS Opticells ESC 00415—EAR 99 2 Kennedy Space Center Fixation Tube (KFT) Main Tube Actuator Expansion Plug Sample Tube 2.365” L x 0.875” D • Provides three levels of containment during all phases • No glovebox required • Samples may be transferred from other growth facilities • Maintains robust containment down to -100 °C For Crew-Facilitated In-Flight Specimen Fixation ESC 00415—EAR 99 Chemical Preservative: •3% glutaraldehyde •5% formaldehyde •5% DMSO •RNAlater™ •b-glucuronidase stain •5% formalin 5% acetic acid 63% ethanol •0.5% glutaraldehyde 2% paraformaldehyde 3 FASTRACK™ Suborbital Platform • Provides compatibility across the entire fleet of emerging suborbital vehicles, existing and future parabolic flight providers, and future orbital free-flyers or transports • Supports 2 single middeck locker equivalents or 1 double locker • Provides power and data acquisition FASTRACK Structural View Parabolic Flight Testing Provides a Standard Platform for Suborbital and Parabolic Investigations ESC 00415—EAR 99 4 Payload Integration & Operations • 52 payloads have been managed and integrated • Platforms: shuttle, ISS, SPACEHAB, Progress, MPLM, Spacelab, Mir • Joint foreign collaborations with CSA, DLR, ESA, JAXA, NSAU • Capabilities for hardware engineering, integration, operations support, and project science coordination • Experiment Monitoring Area (EMA) operations control center at KSC for real time support • ISS Environmental Simulator (ISSES) chambers provide simulated spaceflight environment for analog ground controls Integration History and Capabilities ESC 00415—EAR 99 5 Payload Development: A Phased Approach Pre-Phase A Phase A Phase B Phase C Phase D Phase E • Each phase includes a final milestone or gate • Each phase has distinct activities associated with science, hardware, and analytical integration • Phase duration is a function of many factors – Experiment maturity – Hardware maturity – Platform Payload Lifecycle ESC 00415—EAR 99 6 Payloads in the land of “OZ” Payload Integration Manager Schedule STRATEGIC PDR • • • • Safety Phase 0/I CDR TACTICAL Safety Phase II Export Classification Letter Prelim Design Data Package Safety Data (Phase 0-I) Training Strategy TIMs Payload Pre-Ship Safety Phase III CoFR • Safety Data Pack • Payload Integration Agreement (PIA) • Critical Design Data Package • Safety Data (Phase II) • ICD/Verification Requirements • Resource Requirements Definition • Procedures & Displays DS (P) Key: P – Preliminary U – Update F – Final Payload Manifested • • • • • • • • C&DH DS (P) KSC Support Rqmts DS KSC Technical Rqmts DS Ground Data Services DS Procedures & Displays (U) Payload Planning DS (P) Manifest/Stowage DS (P) Drawing DS (P) • • • • • Manifest/Stowage DS (U) • Drawing DS (U) • Integration Data Pack (IDP) • C&DH DS (F) • Procedures & Displays (F) C&DH DS (U) Payload Trainer H/W & S/W Training Plans & Courseware Payload Planning DS (F) • Interface Control Document (ICD) (U) • Payload Verification Plan (PVP) (U) • Requirements Exceptions Why an Implementation Partner is Important ESC 00415—EAR 99 • CoFR Endorsement 7 Acronyms ABRS BRIC BRIC-LED COTS CSA DLR DMSO ECP EMA ERC ESA EXPRESS GCU GFP ISS ISSES JAXA KFT KSC LSSC MPLM NSAU TOSC Advanced Biological Research System Biological Research in Canisters Biological Research in Canisters – Light Emitting Diode Commercial Off-the-Shelf Canadian Space Agency Deutsches Zentrum für Luft- und Raumfahrt Dimethyl sulfoxide Environmental Control Panel Experiment Monitoring Area Environmental Research Chamber European Space Agency Expediting the Processing of Experiments to the Space Station Generic Containment Unit Green Fluorescent Protein International Space Station ISS Environmental Simulator Japan Aerospace Exploration Agency Kennedy Space Center Fixation Tube Kennedy Space Center Life Science Services Contract Mult-purpose Logistics Module National Space Agency of Ukraine Test and Operations Support Contract ESC 00415—EAR 99 8 Points of Contact Ground Research Support Point of Contact (ESC): Michele N. Birmele Advanced Life Support Laboratories Lead Mail Code ESC-24 Kennedy Space Center, FL 32899 Phone 321.861.2940 Michele.N.Birmele@nasa.gov Laboratory Support Point of Contact (TOSC): Bruce E. Burch TOSC Project Manager Mail Code 721S-S100 Kennedy Space Center, FL 32899 Phone 321.867.5255 Bruce.E.Burch-1@nasa.gov Hardware + Implementation Point of Contact (ESC): David W. Reed Payload Engineering Lead Mail Code ESC-24 Kennedy Space Center, FL 32899 Phone 321.861.2980 David.W.Reed@nasa.gov ESC 00415—EAR 99 9 Thank you! ESC 00415—EAR 99 10