Theresa Kristopaitis, MD
Associate Professor
Director of Mechanisms of Human Disease
Kelli A. Hutchens, MD, FCAP
Assistant Professor
Assistant Director of Mechanisms of Human Disease
Loyola Stritch School of Medicine

• On a peripheral blood smear identify the following cells: erythrocytes, platelets, neutrophils, eosinophils, basophils, lymphocytes and monocytes.
• On a peripheral blood smear identify neutrophil band cells (also known as stab cells) and state the clinical significance of their presence.
• On H&E stained sections, identify neutrophils, eosinophils, lymphocytes.
• On H&E sections identify plasma cells.
• On a stained section identify the 3 major components of bone marrow
(bone trabeculae, hematopoietic cells, adipose tissue).
• List the sequence of development of a granulocytes – focusing on the neutrophil.
• On a section of bone marrow, identify a megakaryocyte.
• Define “blast cell”.

Granulocytes – Peripheral Blood Smear
Neutrophil Eosinophil
Basophil

Agranulocytes – Peripheral Blood Smear
Lymphocyte Monocyte

Red blood cells Platelets

Eosinophils
Neutrophils

Adipose (fat) cells
Bone
Hematopoietic cells

Based on this representative section of normal bone marrow, what is the age the patient?

A B
Granulocytopoiesis
C E G F
Neutrophilic series
A = Myeloblast
B = Myelocyte (large cell, rounded nucleus)
C = Late myelocyte or early metamyelocyte (nucleus beginning to indent)
E = Metamyelocyte (indented nucleus)
G = Band cell (much thinner nucleus)
F = Segmented (mature) neutrophil

Megakaryocyte
(Mega = Giant)
Fragments of cytoplasm break off to become platelets