Biocontrol bacteria and actinomycetes, evaluation of their secondary metabolites against
fungal pathogens and insectpest of chickpea
Meesala Sree Vidya1 and S Gopalakrishnan2
1-Jawaharlal Nehru Technological University, Hyderabad; 2- International Crops Research Institute for the Semi Arid Tropics, Patancheru
Different bacterial isolates inhibiting fungal pathogens.
Introduction
•It is well known that bacteria and actinomycetes found most
common in compost and rhizosphere soils. Hence five different
herbal vermicompost such as Neem, Annona, Gliricidia, Jatropha,
Parthenium and rhizosphere soils from organic fields were
selected for isolating the microbes.
•Bacteria and actinomycetes isolated from above sources were
characterized for their antagonistic activity against fugal
pathogens and insect pests of chickpea. Most potential cultures
will be further characterized for in-vitro PGP and biocontrol traits.
S. rolfsii Vs
VBI-1
Rb-6 Vs
SBI-20
No of actinomycetes isolates antogonistic to different fungal pathogens
Antogonistic Actinomycetes to Fungal pathogens
80
70
60
50
40
30
20
10
0
MP
Rb-6
Objective
Rb-24
Rb-115
Sclerotium
FOC
FM242
FM943
SRI
CONT
Fungal Pathogens
The main objective of the present investigation is to isolate and
identify the active compounds responsible for the inhibition of the
fungal pathogens and H. armigera from the potential antagonistic
microbes and entomopathogens
Charcoal rot
No of bacterial isolates antogonistic to different fungal pathogens
70
Antogonistic Bacteria to Fungal pathogens
60
No of Isolates
Various fungal diseases of Chickpea
Fusarium wilt
FOC Vs
SBI-20
FM-943 Vs
SBI-20
No of Isolates
•In the present investigation antagonistic and entomopathogenic
bacteria and actinomycetes against important fungal pathogens
Sclerotium rolfsii, Fusarium oxysporum f. sp ciceri (FOC), Rhizoctonia
bataticola (3 strains, RB-6, -24 and -115), Fusarium andiyazi (FM943),
Fusarium proliferatum (FM-242), Macrophomina phaseolina (MP) and
insectpest of chickpea, Helicoverpa armigera, were studied.
50
40
30
20
10
0
MP
Rb-6
Rb-24
Rb-115
Sclerotium
FOC
FM242
FM943
Fungal Pathogens
Botrytis gray mold
Dry root rot
Results and Discussion
•Out of 88 actinomycetes isolated, 55, 45, 58 and 37 isolates inhibited MP, Rb6, Rb-24 and Rb-115 respectively; 33, 36, 13 and 13 isolates inhibited FOC, FM242, FM-943 and S. rolfsii respectively.
•From 72 bacteria isolated, 28, 25, 29 and 10 isolates inhibited MP, Rb-6, Rb24 and Rb-115 respectively; 31, 31, 5 and 22 isolates inhibited FOC, FM-242,
FM-943 and S. rolfsii respectively.
Materials and methods
•Bacteria and actinomycetes were isolated from five herbal
vermicomposts and rhizosphere soil from organic field by serial
dilution method.
•Antagonistic activity of the cultures were evaluated by dual
culture assay.
•The promising antagonistic cultures were further characterized
for their PGP and biochemical traits.
•The most promising isolates (20) were further evaluated for their PGP and
biocontrol traits. The results indicated that xx, xx and xx were shown positive
for chitinase, protease, lipase, respectively.
Future work
•Purification of active compound (s) from the culture filtrates.
•Physiological characteristics of the promising cultures.
•Green house and field evaluation of the promising cultures and their
metabolites.
*Corresponding author M. Sree Vidya, Email: vidya.meesala@gmail.com