The discovery of The Chloroplast Julius Von Sachs & Theodore Wilhelm Engelmann By: Jan Villamor Anna- Maria Allen Amanda Brennan Previous Knowledge • Phototropism • Plants take in CO₂ and produce O₂ (from the discoveries of F.F Blackman, Van Helmot, and Jan Ingenhousz) Julius Von Sachs (1832-1897) • • • • A German botanist Fouder of experimental plant physiology Discovered that photosynthesis occurred in the chloroplast Found out that starch is present in chloroplasts by using the Iodine test • It is thanks to him that we have the Iodine test that we use today • Had a great influence on British and American Botany Major experiments • Grew plants in water instead of soil • As a result, it demonstrated that plants can grow with just water, the right amount of nutrients and sunlight • He used the Iodine test to show that carbon was first accumulated as starch in the leaves of plants • Learned that chlorophyll, light and CO₂ are necessary for carbon fixation - carbon fixation is the first stage of the dark reaction of photosynthesis Theodore Wilhelm Engelmann (1843-1903) • A German botanist, physiologist, microbiologist, university professor, and musician • Showed that the light reactions of photosynthesis is converted into chemical energy within the choloroplast • Aim was to discover which part of the cell functioned as light receptors Major Experiments • Used a modified microscope that was able to distinguish and measure different wavelengths of light • Measured the different wavelengths of light on photosynthetic activity • Conducted an experiment that involved illuminating a filamentous alga called Spiragoya (a form of green algae commonly found in freshwater ponds and ditches). -Light was passed through a prism, exposing all the parts of the spiragoya in different wavelengths - aerobic bacteria were used to locate where the alga was releasing the most oxygen - the bacteria clumped under the red and blue light Experiment Findings • Proved that photosynthesis takes place inside the chloroplasts of plants • Photosynthesis is necessary in order for plants to survive • Chloroplasts receive the most energy from red and blue light more than any other colour in the spectrum. A year later, Engelmann discovered that purple bacteria used UV light in the same way Link: Basics of the Chloroplast References: 1) "Julius von Sachs Facts, information, pictures | Encyclopedia.com articles about Julius von Sachs." Encyclopedia - Online Dictionary | Encyclopedia.com: Get facts, articles, pictures, video. N.p., n.d. Web. 13 Nov. 2010. <http://www.encyclopedia.com/topic/Julius_von_Sachs.aspx>. 2) Nair, Sonia. "What is Spirogyra." Buzzle Web Portal: Intelligent Life on the Web. N.p., n.d. Web. 14 Nov. 2010. <http://www.buzzle.com/articles/what-is-spirogyra.html>. 3) "Theodor Wilhelm Engelmann - Wikipedia, the free encyclopedia." Wikipedia, the free encyclopedia. N.p., n.d. Web. 14 Nov. 2010. <http://en.wikipedia.org/wiki/Theodor_Wilhelm_Engelmann>.