Effects of Glucose on the Growth Curve of Pseudomonas fluorescens Muhammad Khan & Andrew Passer November 28, 2011 Our Question: What effect does the addition of glucose have on the growth curve of Pseudomonas fluorescens? Interest in Glucose Experiment 2 Experiment 3 ? ? Experiment 4 Supports Experiment 3 Is glucose metabolism in P. fluorescens acquired or inherited? We could not “shock” P. fluorescens into increased growth by raising it in a glucose-rich, tryptone-poor environment prior to inoculation. Calculating "Growth Points" What Concentration of Glucose Gives the Highest Level of Growth? Average: 3.418% glucose Conclusion • Addition of glucose has a positive effect on the growth of P. fluorescens. • Glucose metabolism is an inherited trait for this species. • Optimum growth occurs at about 3.4% glucose. Discussion & Future Directions • More time needed for P. fluorescens to grow. • Is the prolonged increase in OD actual growth? • What are the waste products of the different metabolic pathways (amino acid vs. sugar)? • How do the waste products affect the stationary phase? Any questions?