IB Internal Assessment (Lab) Scoring DESIGN- Aspect 1 Defining the problem and selecting variables Complete/2 Partial/1 Not at all/0 Formulates a focused problem/research question and identifies the relevant variables. Formulates a problem/research question that is incomplete OR identifies only some relevant variables. Does not identify a problem/research question AND does not identify any relevant variables Examples of scoring: Formulates a focused problem/research question and identifies the relevant variables. • Background Info: Catalase is an enzyme found in nearly all organisms exposed to oxygen. In our cells it is located in peroxisomes & converts harmful hydrogen peroxide into water & oxygen. 1 QUESTION: What is the effect of altering the pH on the function of catalase? – Is this question focused? (Do you know exactly what the experimenter wants to find out?) • Do you know what levels of pH are going to be tested? • Do you know how the “function” of the enzyme will be determined? 1 http://en.wikipedia.org/wiki/Catalase Examples of scoring: Formulates a focused problem/research question and identifies the relevant variables. • Background Info: same as previous slide • QUESTION 2: What is the effect on the rate of oxygen production when hydrogen peroxide and catalase are in a pH environment of 3, 4, 5, 6, 7, and 8? – Is this question focused? (Do you know exactly what the experimenter wants to find out?) • Do you know what levels of pH are going to be tested? • Do you know how the “function” of the enzyme will be determined? Examples of scoring: Formulates a focused problem/research question and identifies the relevant variables. • Background Info: The movement of water from areas of high water availability to areas of lower water availability is an important process in all living things. 1 Without it, water would not enter roots cells from the soil nor would water be able to enter the cells of out body. 2 • QUESTION: How do different concentrations affect osmosis? – Is this question focused? (Do you know exactly what the experimenter wants to find out?) • Do you know what is going to be tested? • Do you know how the movement of water will be determined? 1 http://arbl.cvmbs.colostate.edu/hbooks/cmb/cells/pmemb/osmosis.html 2 -jdldjodgopdgdopd Examples of scoring: Formulates a focused problem/research question and identifies the relevant variables. • Background Info: The movement of water from areas of high water availability to areas of lower water availability is an important process in all living things. 1 Without it, water would not enter roots cells from the soil nor would water be able to enter the cells of out body. 2 • What would you need to know in order to write a focused research question? • Your turn! Use this info. to write one. 1 http://arbl.cvmbs.colostate.edu/hbooks/cmb/cells/pmemb/osmosis.html 2 -jdldjodgopdgdopd What are variables? • any factor, trait, or condition that can exist in differing amounts or types • An experiment has three kinds of variables: – Independent (IDV) what is different in order to test the hypothesis/answer the question – Dependent (DV) the data that is collected • What data are you collecting as you stand at the lab bench? – Controlled these are all the other things that could be different but you want to keep constant • This ensures that the data you collect allows you to answer the question with confidence Aspect 1 variables: IDV & DV • QUESTION 2: What is the effect on the rate of oxygen production when hydrogen peroxide and catalase are in a pH environment of 3, 4, 5, 6, 7, and 8? – Independent (IDV): • Levels of pH (3, 4, 5, 6, 7, 8) – Dependent (DV): • Correct DV example: oxygen levels – at time 0 sec, 30 sec, 60 sec, 90 sec, etc. (this is 4 pieces of data) • Incorrect DV example: rate of oxygen production – (you had to do math to get this #) DESIGN- Aspect 2 Controlling the Variables Complete/2 Designs a method for the effective control of the variables. Partial/1 Designs a method that makes some attempt to control the variables. Not at all/0 Designs a method that does not control the variables. Controlled variables: Aspect 2 • QUESTION 2: What is the effect on the rate of oxygen production when hydrogen peroxide and catalase are in a pH environment of 3, 4, 5, 6, 7, and 8? – Controlled variables: What things can you think of that must be kept constant? Brainstorm with a partner! • • • • • Temperature of H2O2 & catalase Volume/amount of H2O2 & catalase Source of H2O2 & catalase Time intervals oxygen readings are taken Equipment taking the reading – What are things that are not really important to list here? • • • • All IDV levels tested in the same room Same level of humidity outside Same amount of light Same person pouring H2O2 & catalase together Controlled variables are displayed in a chart & they are included in the procedure. Controlled Variables Chart: Controlled Variables (state the variable; no magic #; list what is needed Temperature of enzyme & substrate Y Z How Method Allows for the Control of these Variables (explain how not “why”) Both the hydrogen peroxide & catalase were kept at room temperature (23ºC) Note the use of the metric system! Practice scoring: IB Sample #2 *Before we look at the controls, let’s score Asp1 while we read. QUESTION (this person embedded their question information in a hypothesis) Aspect 2: Controlling the variables • What controls do you see in this procedure? • Same person tasting leaf • Same scale used to rate the leaf bitterness • Mouth cleansing procedure for after eating a leaf (crack & water) • What else could/should have been controlled? • Amount of leaf material tasted/tested • Part of the leaf tasted/tested Practice scoring: IB Sample #3 (Asp 1 first, then Asp 2) QUESTION (this person embedded their question information in a hypothesis) Aspect 2: Controlling the variables Example #3 (pass out procedure handout) • What controls do you see in this procedure? • Test tubes left in water bath for 30 minutes • All apple cube sizes are 1cm3 • Same pH • Same amount of substrate (2 cubes of apple) • Volume of enzyme (pectinase) in each tube • What else is controlled but isn’t listed in the chart? • Total volume in each test tube • Same type of apple • Method of crushing the apple to extract juice DESIGN- Aspect 3 Developing a method for collection of data Complete/2 Develops a method that allows for the collection of sufficient relevant data. Partial/1 Develops a method that allows for the collection of insufficient relevant data. Not at all/0 Develops a method that does not allow for any relevant data to be collected. “Method,” “Sufficient,” “Relevant”: • Develops a Method: Procedure is complete & explains all necessary steps • Sufficient: – at least 5 different levels of IDV – at least 3 trials at each level of IDV (5 is better) • Relevant: – data collected will actually allow you to answer the question • Ex: If you are trying to measure the amount of bacteria that is on your desk as the day progresses & it is used, you must sanitize the desk before you begin the experiment. Aspect 3: Develops a method… tannin in leaves experiment (Ex #2) • All steps included that allow another person to complete the experiment? • No; How were the leaves chosen? • Sufficient Data? • No; only 3 levels of IDV • Not really quantitative; more qualitative (better if he quantified the amount of light) • Relevant Data • Yes; Student is equating bitter tannin taste to level of light & he is tasting for bitterness. Aspect 3: Develops a method… pectinase & pectin (Ex #3) • All steps included that allow another person to complete the experiment? • No; Students Introduction mentioned a rate of reaction for pectinase & the experiment didn’t discuss how to measure the rate of the reaction. • Sufficient Data? • No; only 1 trial completed for each level of IDV (need at least 3; 5 is better) • Reality of how much juice 2 small apple cubes will yield small; apparatus says to use 25mL beaker & this would be too big • Relevant Data • Yes