化脓菌分离鉴定过程(三) 化脓菌分离鉴定过程 葡萄球菌 • 甘露醇发酵试验 • 血浆凝固酶试验 • 耐热核酸酶试验 • 动物试验 甘露醇发酵试验 Inoculate the bacteria into a mannitol micro-tube Incubate at 37℃ for 18h. S.aureus will ferment mannitol to produce acid, which causes the medium to turn yellow. 血浆凝固酶试验(The Coagulase Test) Coagulase • 结合凝固酶 • 游离凝固酶 • 凝固酶 协同因子 纤维蛋白原 凝血酶样物质 纤维蛋白 It has been speculated that this enzyme might be a virulence factor. However, coagulase-negative strains are often as pathogenic as coagulasepositive strains. There are two ways of performing this test • The Slide Coagulase Test • The Tube coagulase Test Tube coagulase Test + - The Slide Coagulase Test 细菌的药物敏感性试验 纸片扩散法(K-B法) 肠道菌分离鉴定过程(三) Antigens of salmonella O antigen H antigen Vi antigen polysaccharide of LPS used to divide Salmonella to 42 groups (A-Z,O51-63,O65-67) A-E groups are pathogenic flagella antigen divide Salmonella tow phase: special no special related to the virulence of Salmonella sensitive to heat ( lose activity at 60℃) Serological Identification of Salmonella Select the specimen React with A-Z polyvalent antiserum Agglutination reaction (+) React with several individual types of anti-O and anti-H antiserum Identify its group and phage (-) React with anti-Vi antiserum 系统实验报告(报告三) 化脓菌/肠道菌分离鉴定过程 目的 策略(原理设计) 方法步骤:简单写 结果(判定方法) 结论