Tumaini (CRT) Inc.
Efficacy of a New Slow
Release Mosquito
Larvicide containing
Novaluron
SLOW RELEASE MOSQUITO LARVICIDE DETAILS
 Larvicide contains the active ingredient novaluron and utilizes novel
formulation technology which has been patented in numerous countries
 Formulation controls rate of release of novaluron into water column
 increases solubility of novaluron in water from 3 ppb to 10-15 ppb
 Formulation also promotes dispersion of novaluron throughout the
water column
 products will be available in several different sizes and shapes to address
different market needs
 all products have same recipe and ai 1.2g/kg novaluron
 Tradename for larvicide products are:
Mosquiron 0.12P and Mosquiron 0.12CRD
MOSQUIRON PRODUCT DETAILS
Mosquiron 0.12P pastilles (2 sizes)
Mosquiron 0.12CRD
controlled release device
MOSQUIRON PRODUCT DETAILS
Mosquiron 2010 Field Trials
PURPOSE:
 Assess efficacy and duration of Mosquiron products when used
under simulated field conditions and in different regions of the
US and Canada
Mosquiron 2010 Field Trials
Experimental Design
 Needed to take into consideration the mode of action of novaluron
 Novaluron is a chitin synthesis inhibitor and effects can be cumulative;
larvae can appear healthy and mortality may not be observed until
adults emerge from pupal case
 At high concentrations of novaluron (>5 ppb) effect on larvae can be
rapid – 24 hours
 Mortality can still occur at pupal eclosion caused by incomplete
separation from pupal case – adult drowning
Field Trial Locations
 Canada – Portage la Prairie MB, Abbotsford BC
 US – Marshall WI, Meansville GA, Panama City FL
Two non-treated checks were
included in the experiment, and ALTOSID XR-Briquettes (active ingredient: (S)Methoprene) were also tested as a positive control. ALTOSID XR-Briquettes (EPA
Registration No.: 2724-421) were purchased from Central Life Sciences.
Mosquiron 2010 Field Trials
Experimental
Design
Table 1. Products and Rates Tested
Treatment
Number
Treatment
Name
Amount/ Type of
Formulation
Minimum
Amount of
Water (L) added
1
Control-A
0
200
Type of Organic
Matter *
Mosquito food only
Sod grass &
2
Control-B
0
200
mosquito food
Sod grass &
3
Positive Control
1 Altosid XR Briquette
200
mosquito food
Sod grass &
4
Pastille 0.5X
20 grams of pastilles
200
mosquito food
Sod grass &
5
Pastille 1X
40 grams of pastilles
200
mosquito food
Sod grass &
6
Pastille 2X
80 grams of pastilles
200
mosquito food
7
CRD 0.5X
½ CRD**
200
Mosquito food only
8
CRD 1X
1 CRD
200
Mosquito food only
9
CRD 2X
2 CRDs
200
Mosquito food only
* The FL-1 site utilized existing concrete tanks with soil and vegetation established in the bottom of the
tanks for Treatments 2 through 6.
** CRDs were cut in half for the 0.5X rate.
Galvanized metal tanks (approximate capacity of 250 liters) were used at all sites except
FL-1. The FL-1 site utilized permanent concrete tanks. For treatments 2 through 6, a
Mosquiron
Table 3. Mosquito
Egg Sources and2010
Genera
Field Trials
Trial No.
Egg Source
Genera
BC-1
From wild mosquitoes laying eggs in
small artificial pools.
Culex spp.
FL-1
Reared and harvested by Ken Shaffer at the Public
Health Entomology Research and Education Center,
Panama City, FL
Culex
quinquefasciatus
GA-1
Reared and harvested by Steve Sackett, New Orleans
Mosquito and Termite Control Board (NOMTCB).
Aedes aegypti
MB-1
From wild mosquitoes laying eggs in
small artificial pools.
Culex spp.
WI-1
From wild mosquitoes laying eggs in
small artificial pools.
Culex restuans and
Culex pipiens
Mosquiron 2010 Field Trials
Experimental Design
 Eggs were introduced to treatment tanks approx . every 2 weeks
 Culex eggrafts were placed directly on water surface at WI, MB, BC and
FL sites
 Once hatching occurred all trts. received a larval liquid food slurry
 Food slurry was a standard formula consisting of liver powder/brewer’s
yeast
 Food administered 3-4 times/wk. until water was cloudy in appearance
Mosquiron 2010 Field Trials
Marshall - WI site
Meansville - GA site
Mosquiron 2010 Field Trials
Panama City -FL Site
Mosquiron 2010 Field Trials
Portage la Prairie - MB Site
Mosquiron 2010 Field Trials
Abbotsford - BC Site
Mosquiron 2010 Field Trials
Data Collection
 Treatments were inspected for the presence of live larvae and/or pupae
on an as needed basis. Once 3rd and/or 4th instars were present, dips
were taken to harvest live larvae.
 Florida A&M’s PHEREC Standard Mosquito Sampling Method was used
at all trial sites.
 Using a standard mosquito dipper, eight dips were taken per treatment.
 The number of larvae and/or pupae per dip were counted and
recorded. Larvae were returned to the treatment tanks.
 Once pupae were found, they were transferred to emergence jars for
monitoring.
 Adults that emerged successfully from pupal cases were counted and
recorded as successful hatches. Dead adult mosquitoes on the water
surface recorded as unsuccessful emergence.
Observations
 Using local sources for harvesting Culex eggrafts delayed start of trial in
Northerly sites to early July
 Was exacerbated by a cool wet spring in Canada and WI
 FL location received very high precipitation (18” rain in July) causing
significant dilution of novaluron in cement troughs
Conclusions
 Study results demonstrate Mosquiron formulations provided 100% IE
for duration of study at all locations
 100% IE observed at FL site with 2x CRD