Automated Mastitis Detection for Dairy Farms Amanda Sterrett & Jeffrey Bewley University of Kentucky Dairy Systems Management But how we monitor it is different… Take advantage of simplicity Because cows are routine-oriented, we can monitor their behavior and examine differences in: Eating time / DMI Standing / Lying time Rumination time Location within barn Physiological monitoring Body temperature Ear, milk, reticulorumen, udder, vagina Milk composition SCC Fat, lactose, protein, LDH, etc. Electrical conductivity Potential Benefits Early Mastitis Detection Early Treatment Improved Treatment Outcome Less Production Loss Improved Prevention Program Less Economic Loss Improved Animal WellBeing Accuracy and Precision Sensitivity and Specificity Sensitivity (true positive rate): alert with an observed mastitis case 𝑡𝑟𝑢𝑒 𝑝𝑜𝑠𝑖𝑡𝑖𝑣𝑒𝑠 𝑆𝑒𝑛𝑠𝑖𝑡𝑖𝑣𝑖𝑡𝑦 = 𝑡𝑟𝑢𝑒 𝑝𝑜𝑠𝑖𝑡𝑖𝑣𝑒𝑠 + 𝑓𝑎𝑙𝑠𝑒 𝑛𝑒𝑔𝑎𝑡𝑖𝑣𝑒𝑠 Specificity (true negative rate): no alert with no mastitis 𝑡𝑟𝑢𝑒 𝑛𝑒𝑔𝑎𝑡𝑖𝑣𝑒𝑠 𝑆𝑝𝑒𝑐𝑖𝑓𝑖𝑐𝑖𝑡𝑦 = 𝑡𝑟𝑢𝑒 𝑛𝑒𝑔𝑎𝑡𝑖𝑣𝑒𝑠 + 𝑓𝑎𝑙𝑠𝑒 𝑝𝑜𝑠𝑖𝑡𝑖𝑣𝑒𝑠 Electrical Conductivity Ion concentration of milk changes during mastitis Inexpensive and simple equipment Wide range of sensitivity and specificity reported Affected by sample time, milk viscosity, temperature, and sensor calibration Most useful when combined with other data Automated CMT or WMT • CellSense (New Zealand) • r = 0.76 with Fossomatic SCC Alert based on EC Alert based on InLine SCC Alert based on EC and SCC Alert time period Observation period Sensitivity False alert rate Sensitivity False alert rate Sensitivity False alert rate 96 48 80 4.7 83.3 2.9 80 1.2 48 24 80 7.8 83.3 3.7 80 2.1 Somatic Cell Count • In-line detection of cell count, milk temperature, and electrical conductivity • Uses ATP luminescence as an indicator of the number of somatic cells • Sensor connected to the milk hose below the milking claw • Reagent cassette attached below display Spectroscopy • Visible, near-infrared, mid-infrared, or radio frequency • Indirect identification through changes in milk composition • AfiLab uses near infrared – Fat, protein, lactose, SCC, and MUN LDH Threshold (µmol min-1|-1) Sensitivity Specificity 4.3 95.2 92.0 6.5 72.6 98.5 http://blog. modernmec hanix.com/r obot-cowmoos-andgives-milk/ Temperature Limitations • Not all cases of mastitis result in a temperature response • Best location to collect temperature? • Noise from other physiological impacts Udder Thermography • Udder temperature closely related to rectal temperature • No early detection in LPS challenge (Hovinen et al., 2008) • Potential use in dry cows Before Infection After Infection Hovinen et al., 2008 Accelerometers • Measures lying time and activity/motion index • Well researched and applied to many areas • Lying is a high priority behavior • May change lying time around mastitis • May decrease activity around mastitis • Lying time decreased by 73 minutes on the day of challenge (P < 0.01, Cyples et al., 2012) Rumination Behavior • Cows with mastitis may ruminate less • r = 0.93 for automated rumination with live observations in cows (Schirmann et al., 2009) Animal Position • Real Time Location System • Cows may stay in same spot longer around mastitis Multi-parameter Sensors • Combination monitors may find a better market than those sensors only targeted at one parameter: – Temperature – Activity – Rumination – Feeding Time • Multivariate analyses Considerations Economics Positive return on investment Producer satisfaction What data is useful? Reading frequency What do we do with the data? Culture, monitor, treat, ignore? Conclusions Using technologies for mastitis monitoring is newer than using them for estrus detection Algorithms are not yet perfected Continued research is needed, particularly in naturally occurring mastitis Questions? Amanda Sterrett 408 WP Garrigus Building Lexington, KY 40546 412-558-2075 amanda.sterrett@uky.edu Dr. Jeffrey Bewley 407 WP Garrigus Building Lexington, KY 40546 859-699-2998 jbewley@uky.edu