Estimating Time of Death Forensics 2014-1015 Livor Mortis ”Death Color” • As body begins to decompose blood seeps down through tissues and settles in lower parts of body – Red Blood Cells Turn purplish-blue • Pooling of blood = lividity • Lividity Begins about 2 hours after death • Between 2-8 hours lividity will be present, but if the skin is pressed color will dissappear • After 8 hours lividity remains if the skin is pressed • Ambient Temperature = Temperature surrounding body – affects the rate of lividity • Higher Temperature = Increase in rate of lividity • Lower Temperature = Decrease in rate of lividity Livor Mortis • What else can be determined from lividity? – Position of body following death – Whether a body has been moved post mortem Algor Mortis death heat • The Chill of Death – Algor Mortis-Describes temperature loss in a corpse • Rate of loss influenced by environmental conditions – Exterior temps – Clothes vs no clothes ect… – First 12 hours. • Temperature loss = .78 degrees Celsius (1.4 degrees Fahrenheit) per hour – After 12 hours • Temperature loss = .39 degrees Celsius (.7 degrees Fahrenheit) per hour Muscle Fibers and Contraction Rigor Mortis “Death Stiffness” • Normal Muscle Contraction – Calcium released from membranes inside cell causes muscle fibers cells to contract – Active transport then removes calcium so muscle fibers can relax • This requires oxygen and an energy supply • The Rigidity of Death – Stiffness occurs because skeletal muscles are locked in a flexed position and are unable to relax. – Calcium leaks out of cell and muscle remains in a contracted position • No active transport after death • Starts within 2 hours of death – Begins with Head (Face) and neck • Peak Rigor, At 12 hours the body is at its most rigid state – At about 15 hours muscle fibers break down and soften • Stiffness gradually disappears after 36 hours • Rigor may remain for up to 48 hours Rigor Mortis Observation Approx. Time Scale The body is at its most rigid state Just over 2 hours No visible signs of rigor Less than 2 hours or more than 48 hours ago Stiffness generally disappears After 36 hours Factors Affecting Rate of Rigor • Ambient Temperature – Cold- slows rigor – Warm- Accelerates Rigor • Clothing or Lack of Clothing – Clothes = accelerates rigor – No Clothes = slows rigor • Sun Exposure – Accelerates rigor • Activity/Exercise – Exercising or struggling = accelerates rigor – Sleep = slows rigor • Body Weight – Obese- slows rigor (fat stores oxygen) – Thin- Accelerates Rigor Stomach and Intestinal Contents • If undigested stomach contents are present, then death occurred 0 to 2 hours after last meal • If the stomach is empty but food is found in the small intestine, then death occurred at least 4-6 hours after last meal • If the small intestines is empty and wastes are found in large intestine, then death probably occurred 12 or more hours after last meal Decomposition • The rotting or break down of all tissues and organs – Bacteria and other microorganisms aid the decomposition process, just as they decompose plants and animals in the environment. • Occurs in Predictable Pattern – Can be used to help determine Time of Death Stages of Decomposition • Initial Decay – Corpse appears normal from the outside, but is starting to decompose from the actions of bacteria and autolysis • Putrefecation – Odor of decaying flesh is present and the corps e appears swollen • Black Putrefecation – Very Strong odor. Parts of the flesh appear black. Gases escape and the corpse collapses • Butyric Fermentation – Corpse is beginning to try out. Most of the flesh is gone • Dry Decay – Corpse is almost dry. Further decay is very slow from lack of moisture Timeline of Events during Decomposition • • • Within 2 days after death: – Cell Autolysis begins following death – Green and purplish staining occurs form blood decomposition – The skin takes on a marbled appearance – The Face Becomes discolored After 4 Days: – The skin blisters – The abdomen swells with the gas carbon dioxide release by bacteria living in the intestines Within 6-10 Days: – Corpse bloats with carbon dioxide, eventually gas causes the chest and abdominal cavities to burst and collapse – Fluids begin to leak from body openings as cell membranes rupture – Eyeball and other tissues liquefy – The Skin sloughs off Factors Affecting Rate of Decomposition • Temperature – Bodies decompose fastest within 21-37 degrees Celsius (70-99 degrees Fahrenheit) – Below this range rate decreases because cold temperatures slow the growth of bacteria and microorganisms – Above this range tends to dry out corpses and preserve them • Several other factors – – – – Illness Age Weight Clothing or Lack of Clothing Forensic Entomology • Forensic entomologist – Collects insect evidence from on, above, and below the body – Records environmental conditions – Because life cycles are affected by fluctuations in the daily environmental conditions, insects cannot provide an exact time of death, only a close estimate. – At crime lab some of the insects are raised under environmental conditions that mimic the environmental conditions at the crime scene to obtain most accurate approximation Forensic Entomology • Blowflies are usually the first insects to arrive at a dead body – Usually arrive within minutes • Blowflies exhibit different life stages – Egg, larva (also known as instars), pupa, prepupa, adult Egg • Soon after Death—blowfly eggs can be found in the moist, warm areas of a corpse – < 8 hrs Larva Stages 1 (Instar 1) • Within 20 hours Larva 2 (Instar 2) • After 2 ½ days Larva Stage 3 (Instar 3) • After 4-5 days Pre-Pupa • After 8-12 days Pupa • After 18-24 days Adult • After 21-24 days Time of Death—Insects o The insect life cycle provides scientists with a benchmark to estimate a time of death o Insect evidence cannot provide an exact time of death—fluctuating environmental conditions o Insect evidence provides a close estimate