Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange Lesson 10.1 Gas Exchange in Plants Contents Introduction 1 Learning Objectives 2 Warm Up 2 Learn about It! Gas Exchange General Principles for E cient Gas Exchange Gas Exchange in Plants Gas Exchange in Leaves The Stomata General Mechanism of Stomatal Function Role of Potassium Ions in Stomatal Opening Stomatal Gas Exchange Role of Abscisic Acid (ABA) in Stomatal Closing Gas Exchange in Roots and Stems Root Hairs Lenticels 4 4 4 6 7 8 8 9 11 12 14 15 16 Key Points 17 Check Your Understanding 19 Challenge Yourself 20 Photo Credits 21 Bibliography 21 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange Lesson 10.1 Gas Exchange in Plants Introduction Can you identify the green structures found in the picture above? No, they are not pairs of beans or peas. They are also neither eyes nor copies of green Mike in the Disney animated movie, Monsters, Inc. But if you identify them as stomata, then, you are right. The photo gives a view of a leaf specimen viewed under the microscope, which shows the stomata as these green bean-like structures surrounding a tiny central hole. Most probably, you have already seen them in your junior years in high school and learned their roles in plant maintenance and survival. 10.1. Gas Exchange in Plants 1 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange By this time, you already know that living organisms, including plants, obtain the energy they need from specific biochemical processes. One of these processes is respiration, which allows animals to acquire the gases they need from the atmosphere. Like animals and other organisms, plants do “breathe,” but not in the same manner as animals. This requirement is when stomata come into play. Stomata are essential leaf structures that allow plants to “breathe.” The question now is, “how?” To answer the “how” question, you must know the general principles that govern efficient gas exchange to understand fully how plants and animals respire. Then, as you progress through the lesson, you will learn the mechanism by which stomata work to allow for plant respiration. You will also discover other “breathing” structures in plants. Learning Objectives In this lesson, you should be able to do the following: ● Identify the factors that affect DepEd Competency Compare and contrast the process of gas exchange in plants and animals (STEM_BIO11/12 -IVa-h-1). gas exchange efficiency. ● Discuss the mechanism of stomatal opening and closing. ● Identify other plant structures that facilitate gas exchange. ● Explain how plants make their gas exchange efficient. Warm Up Leaf in Hot and Cold Water 10 minutes The leaves of plants are occasionally subjected to changing temperatures. This trend affects how leaf structures react, thereby affecting plant respiration. In this activity, you will 10.1. Gas Exchange in Plants 2 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange investigate how temperature affects one of the essential leaf structures needed by plants for “breathing.” Materials ● 2 transparent glass cups (or beaker, if available) ● hot water ● cold water ● 2 leaves of the same plant (preferably of the same size) Procedure 1. Prepare set-ups of hot and cold water in separate beakers or cups. Make sure they have the same amount of water. 2. Submerge a leaf into each glass containing water of different temperatures. Refer to Fig. 10.1.1. as a guide. Fig. 10.1.1. Leaves submerged in hot and cold water 3. Wait for one minute and observe bubble formation in your set-ups. Notice the part of the leaf where these bubbles form. 4. Let the beaker with cold water sit for another five minutes. Observe if any bubbles are forming on the leaf surface. 5. Discuss with the group the implications of your observations. Be ready to share your ideas in class. 10.1. Gas Exchange in Plants 3 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange Guide Questions 1. Which temperature allowed for the bubble formation? 2. Which side of the leaf did the bubbles form? 3. What structure of leaf can be associated with the formation of bubbles in the set-up? How does this relate to the location and number of this structure in the leaf? 4. How does temperature affect the opening and closing of the stomata concerning the bubble formation on the leaf? 5. Based on the activity, which time of the day (day and night) do you think the stomata open and close? Learn about It! Gas Exchange All living organisms obtain their energy by metabolizing certain compounds. Metabolism is a collection of all life-sustaining biochemical processes. One primary biological function that involves complex reactions is respiration. Respiration releases chemical energy stored in glucose molecules, and it requires oxygen and releases carbon dioxide as a by-product. The gas exchange facilitates the movement of these gases across cells and between cells and the external environment. In the warm-up activity, the bubbles generated under the leaf showed the flow of gas molecules from the leaf tissue to its environment. General Principles for Efficient Gas Exchange Organisms have various means to obtain the gases they need, but the underlying mechanism that governs gas exchange is diffusion. Diffusion is the movement of molecules down their concentration gradient, allowing them to move from an area with high concentration to one with lower concentration. In living organisms, diffusion requires that the surfaces across which gas exchange occurs must be moist. The rate of gas exchange by diffusion or how fast molecules move across moist membranes is primarily affected by four factors discussed in Fick’s law. This law, developed by Adolf Fick in 1855, describes the main factors that affect the diffusion rate, which consequently affects the efficiency of gas exchange. Recall from a previous lesson that 10.1. Gas Exchange in Plants 4 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange when the diffusion rate is high, the effectiveness of gas exchange increases, allowing organisms to generate more energy. Table 10.1.1. Factors affecting the diffusion rate according to Fick’s law Factor Relationship with Diffusion Description Surface Area of the Membrane Direct A greater membrane surface area can accommodate more molecules for diffusion, which increases the diffusion rate. Concentration Gradient Direct The concentration gradient occurs when a difference in the concentration of molecules is present between two areas. This difference allows for the downhill movement of molecules. The larger the difference in concentration (or the steeper the gradient), the faster the particles move. Thickness of the Membrane Inverse A thicker membrane reduces the diffusion rate, whereas a thinner membrane increases the rate at which the particles diffuse. Inverse Particles that move over a large distance have a lower diffusion rate than those moving over a shorter distance. Distance of Diffusion Based on the factors that affect diffusion, when do we say that the gas exchange in living systems is e cient? Different organisms have different cell sizes and structures that affect gas exchange efficiency. Unicellular organisms do not have any specialized respiratory organs. Because of their small size, thin cell membrane, and constant contact with their environment, gases diffuse into their cells through the membrane itself with the short distance covered. This feature makes the gas exchange more efficient. By contrast, multicellular organisms are larger, which relatively decreases the diffusion rate. However, if they are thin and flat 10.1. Gas Exchange in Plants 5 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange (regardless of size), their body’s outer surface is sufficient to make gas exchange efficient. Large multicellular organisms need to have specialized respiratory organs to improve the gas exchange efficiency in their bodies. Thus, the effectiveness of gas exchange increases with a large surface area for diffusion, high concentration gradient, thin membrane through which gases move, and short distance over which diffusion occurs. What makes the gas exchange in plants different from the gas exchange in other organisms? Gas Exchange in Plants In the strictest sense, plants do not breathe because breathing is the mechanical inhalation and exhalation of gases through a respiratory organ. It is just an analogy used to say that gas exchange occurs in plants. They absorb carbon dioxide for photosynthesis and release oxygen as its by-product. It may seem that oxygen is a waste product of plants, but they also need oxygen to survive. Unlike animals and humans, plants do not have specialized respiratory organs, like lungs and gills, that allow them to breathe. Instead, gas exchange in plants occurs not only in one organ—it happens in their leaves through stomata, stems through lenticels, and roots through root hairs. Stomata in leaves (left), lenticels in stems (center), and root hairs (right) are structures in plants that facilitate gas exchange. 10.1. Gas Exchange in Plants 6 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange Gas Exchange in Leaves The leaves of plants have a very vital role in carbohydrate synthesis and utilization through photosynthesis and respiration. Since these two processes require gases, leaf structure is well adapted for gas exchange. Its lower epidermis has two features that allow for the flow of gases: the spongy mesophyll, which is loosely packed to facilitate gas exchange in the leaf tissue, and stomata (singular, stoma or stomate), which allow for the entry and exit of gases to and from the leaf. Fig. 10.1.2. shows the location and structure of stomata and spongy mesophyll. While both leaf structures are essential for gas exchange, we will focus on the structure and function of stomata related to the mechanism of gas exchange. Fig. 10.1.2. The spongy mesophyll and stomata are structures that facilitate the gas exchange in leaves. How do stomata determine when to open and close for gas exchange? 10.1. Gas Exchange in Plants 7 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange The Stomata On the outer layer of the leaf, particularly on the lower epidermis, are microscopic holes called stomata, which regulate the gas exchange and loss of water by opening and closing. Each stoma is surrounded by two bean-shaped guard cells that are attached at both ends. The thick and elastic lateral edges of these cells do not touch each other to allow them to expand and contract as they regulate the aperture or opening of the stoma. Guard cells are also bordered by epidermal cells called subsidiary cells. The stoma, a pair of guard cells, and the subsidiary cells comprise the stomatal complex, as illustrated in Fig. 10.1.3. Fig. 10.1.3. A stomatal complex consists of a stoma, two guard cells, and several surrounding subsidiary cells. General Mechanism of Stomatal Function Generally, the stoma opens during the day because of two external factors: light and warm temperature. By contrast, it closes during the night due to the low temperature and absence of light. However, stomatal opening and closing are primarily determined by how well the plant is hydrated. The change in the turgor pressure (pressure caused by water) of guard cells causes them to swell or shrink. Fig. 10.1.4. illustrates the structure of the stoma and guard cells when the turgidity is high and low. 1. When guard cells take up water through osmosis, their turgidity increases, causing them to expand while keeping both of their ends attached. Consequently, the stoma opens. 10.1. Gas Exchange in Plants 8 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange 2. When guard cells lose water, their turgidity decreases, causing them to shrink and become flaccid. The inner walls of the guard cells touch each other resulting in the closing of the stoma. Fig. 10.1.4. Stoma opens when turgor pressure in guard cells increases and closes when the pressure decreases. Role of Potassium Ions in Stomatal Opening Water does not just move into the guard cells of stomata to regulate the turgor pressure. Potassium ions (K+) have a critical role in this movement of water. From the plant’s xylem, water molecules move into the palisade and spongy cell layers of leaves and then into the subsidiary cells. The following events take place as blue light triggers stomatal opening. 1. Blue light reception. When light strikes the leaf during the daytime, photosynthesis begins, and the stomatal opening is triggered, specifically by blue light. The guard cell photoreceptor protein, called phototropin, acts as a blue light receptor. 10.1. Gas Exchange in Plants 9 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange 2. H+-ATPase activity. Upon exposure to blue light, the H+-exporting ATPase in the plasma membrane of guard cells becomes activated and pumps out hydrogen ions (H+), making the guard cells hyperpolarized or more negative. 3. Potassium ion uptake. The hyperpolarization of the membrane of guard cells results in the uptake of K+ from the surrounding subsidiary cells through potassium ion channels. 4. Decrease in water potential. The increase in the K+ concentration of guard cells results in a decrease in their water potential. Water potential is the measure of water molecules’ tendency to move from a hypotonic solution (more water, less solute concentration) to a hypertonic solution (less water, higher solute concentration). Consequently, water molecules stored in the subsidiary cells enter the guard cells by osmosis. 5. Stomatal opening. The movement of water increases the turgor pressure and shape of guard cells, resulting in stomatal opening. Fig. 10.1.5. summarizes the process of stomatal opening. Fig. 10.1.5. The mechanism of the stomatal opening through blue light reception 10.1. Gas Exchange in Plants 10 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange Stomatal Gas Exchange Once the stomata of leaves are open, the intercellular free spaces around the spongy mesophyll become in contact with the gases from the environment. The concentration and pressure of carbon dioxide outside the leaf are higher than within these free spaces. In comparison, the concentration and pressure of oxygen inside the leaf are greater than in the environment. The principle of diffusion dictates that the movement of particles is down their concentration gradient. Thus, oxygen gas, including water vapor, will exit the leaf, and carbon dioxide will enter through stomata. This exchange of gases—carbon dioxide in and oxygen out—primarily occurs during the light-dependent phase of photosynthesis. However, in respiration, plants also need oxygen. If plants need oxygen, then why do they release it? Not all of the oxygen produced in photosynthesis is expelled because some are used for respiration. Also, when sufficient light is present, the rate of photosynthesis exceeds that of respiration. Thus, they absorb carbon dioxide more than they emit. This feature results in a net increase in oxygen production and carbon dioxide usage. Therefore, overall, plants take in carbon dioxide and give out oxygen. This exchange of gases will continue to occur as long as the stomata of leaves remain open. Did You Know? Not all plants open their stomata during the day and close them at night. Recall from a previous lesson that plants can be classified as C3, C4, or CAM species according to their carbon fixation pathways. Most C3 and C4 plants open their stomata during the day and close them at night. On the other hand, CAM (Crassulacean Acid Metabolism) plants, as an adaptation to extremely hot conditions, prevent excessive water loss by closing their stomata during the day and opening them at night. Cacti are an example of plants that perform CAM photosynthesis. 10.1. Gas Exchange in Plants 11 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange Pineapples are another example of a CAM plant. Role of Abscisic Acid (ABA) in Stomatal Closing Stomata are light-mediated structures. Without light input, guard cells start to contract, leading to the closing of stomata. However, stomatal closing does not just happen as the day turns into night. An inhibitor for the uptake of K+, particularly the abscisic acid (ABA), is required. Studies have linked the accumulation of ABA and CO2 in guard cells in the dark as a critical player in stomatal closure. 1. CO2 accumulation. In the absence of light, the rate of respiration becomes relatively higher, which results in the accumulation of CO2 in the leaf tissue. Water also becomes available for reaction with CO2 to produce carbonic acid (H2CO3). 2. ABA accumulation. The decrease in the pH (increase in acidity) of the cell due to the accumulation of H2CO3 activates ABA. 3. Ca2+ release from vacuoles and K+ uptake inhibition. The accumulation of ABA in guard cells stimulates the release of calcium ions (Ca2+) from internal stores, such as the vacuoles and endoplasmic reticulum, and by opening Ca2+ channels in the membrane. Consequently, Ca2+ concentration in the cytosol increases. Also, ABA inhibits further K+ uptake by the cell. 4. Membrane depolarization. Ca2+ movement outside of the cell results in plasma membrane depolarization (less negative), which causes K+ and other solutes to move out of the guard cells and back into the subsidiary cells. 5. Stomatal closure. The water potential inside guard cells increases. The movement of water back into the subsidiary cells means that guard cells will lose 10.1. Gas Exchange in Plants 12 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange turgidity, shrink, and close the stoma. Fig. 10.1.6. provides a summary of the mechanism of ABA-mediated stomatal closing. Fig. 10.1.6. The mechanism of ABA-mediated stomatal closing As a general rule, the absence of light closes the stomata during the night. Thus, light-dependent reactions are also not possible. While these structures and reactions are affected by the absence of light, gas exchange and respiration in plants continue to occur even at night. You may wonder how gas exchange and respiration occur even when the stomata are closed. In the dark, most plants close their stomata, but not so entirely that oxygen cannot diffuse into the leaf. The tiny gap left between the guard cells is big enough to allow the small gas molecules to pass through. Since the overall rate of photosynthesis slows down at night and respiration remains fairly constant, there is a net increase in oxygen uptake and a net increase in carbon dioxide production. Consequently, the release of carbon dioxide and the uptake of oxygen is relatively greater at night. Why do you think the stomata need to close and cannot remain open even when they are vital for gas exchange? 10.1. Gas Exchange in Plants 13 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange Did You Know? An open stoma not only allows gas exchange, but it also provides an opening through which harmful pathogens, like bacterial cells, can invade the leaf. However, guard cells have receptors for pathogen-associated molecular patterns (PAMPs) that can detect molecules associated with pathogens. When they recognize these PAMPs, the abscisic acid (ABA) mediates the stoma’s closure to prevent pathogens from entering the leaf. This mechanism is called innate immunity or the immediate prevention of foreign bodies’ spread throughout the body of an organism. This mechanism also resembles the innate immune responses in animals, such as the skin and specific blood cells. Gas Exchange in Roots and Stems The stomata in leaves are the main structures that allow gas exchange in plants. However, cellular respiration takes place in all plant cells. Since the metabolism and gas transport from leaves to different plant organs is slower than that of other organisms, plants need to have additional structures to help them efficiently obtain the gases from the atmosphere. Young plants, including those with green stems, have stomata embedded in their stems, whereas woody plants have their lenticels. Roots also play a role in gas exchange, specifically via their root hairs. 10.1. Gas Exchange in Plants 14 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange Root Hairs Aside from absorbing water from the soil, the roots also permit the exchange of gases through its root hair. Root hairs are single-celled extensions of the root epidermis. This structure gives the root hairs the permeance for water, minerals, and oxygen. Fig. 10.1.7. illustrates the structure of root hairs. Through diffusion, oxygen molecules from the spaces between soil particles diffuse into the thin membrane of the root hairs. These molecules then reach all the cells of the root where they are utilized for respiration, while the carbon dioxide produced goes out through the same root hair. Fig. 10.1.8. Pneumatophores function for gas exchange and are usually found in plants that thrive in swamps or mangrove communities. Shown in this photo are the pneumatophores that stick up from the substrate. These roots come from the mangrove tree shown. In cases where a plant’s habitat becomes flooded or over watered for an extended period, the plant may die because too much water displaces the gases in the soil, making oxygen unavailable to the roots. Pneumatophores, modified aerial roots that are specialized for gas exchange, are well adapted to this condition. They are also called “breathing tubes” because of their role in gas exchange in a water-logged condition. They are usually found in hydrophytic plants whose roots are submerged underwater, like those of the mangrove 10.1. Gas Exchange in Plants 15 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange species. The pneumatophores of these hydrophytes (i.e., plants that are completely or partially submerged in water) tend to protrude themselves above the surface of the water or water-logged substrate where they can be properly aerated. A sample picture of pneumatophores is shown in Fig. 10.1.8. Lenticels Mature woody stems are impervious to gases and water because they are sheathed with dead cells covered with a waxy waterproof and airproof substance called suberin. However, these layers of dead cells are perforated by non-suberized pores called lenticels. Lenticels (from the Latin word lenticella, small window) are raised porous tissues consisting of cells with large intercellular spaces in the periderm of plants with woody stems. They are usually round, oval, or elongated in structure. These porous tissues are filled in with complementary cells from the meristematic or dividing tissue of the cork cambium during the secondary growth of woody stems. Fig. 10.1.9. shows an illustrated and actual structure of lenticels found on a tree. Lenticels are analogous to stomata as they permit the exchange of vital gases, such as CO2, O2, and water vapor, between the environment and the internal tissue spaces in stems. But unlike stomata, lenticels remain open both day and night as they do not have guard cells to regulate their aperture. Fig. 10.1.9. The cells that fill in and surround the lenticels (left) and the structure of lenticels on a tree trunk (right) 10.1. Gas Exchange in Plants 16 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange Based on the gross structure of leaves and the principles governing gas exchange, how do you think do plants make their gas exchange e cient? Key Points __________________________________________________________________________________________ ● Diffusion is the movement of particles from regions of higher concentration to regions of lower concentration. It is the fundamental mechanism that governs gas exchange in organisms. ● Gas exchange in living systems occurs via diffusion across a moist membrane. It provides a means for the movement of gases between the cells and their environment. ● The rate of diffusion affects the efficiency of gas exchange. This rate is influenced by four main factors described by Fick’s law. ○ The concentration gradient and surface area of the respiratory membrane have a direct relationship with the diffusion rate. By contrast, membrane thickness and the distance covered by particles have an inverse relationship with the diffusion rate. ● Gas exchange in plants mainly occurs in epidermal openings called stomata. Each stoma is flanked by guard cells that regulate its aperture. ● The mechanism of stomatal opening and closing is triggered by blue light and mediated by potassium ions and abscisic acid. The figure on the next page summarizes this mechanism. 10.1. Gas Exchange in Plants 17 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange ● Once the stomata open, the concentration gradient between the environment and the leaf will facilitate the movement of gases. ● While some oxygen molecules generated in photosynthesis are used in respiration, photosynthesis occurs at a relatively higher rate than respiration. This event brings a net increase in oxygen production and a net increase in carbon dioxide usage. Thus, overall, plants take in carbon dioxide and give out oxygen. ● At night when stomata are closed, a tiny gap is left between the guard cells, enough for oxygen to diffuse into the leaf. ● Since all plant cells perform respiration, and the gas transport into other plant organs is relatively slower than in other organisms, additional structures such as lenticels and root hairs supplement the gas exchange in plants. __________________________________________________________________________________________ 10.1. Gas Exchange in Plants 18 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange Check Your Understanding A. Identify the correct term being described in each of the following items. 1. This plant hormone prevents the potassium ion uptake by guard cells. 2. These microscopic openings in the lower epidermis of leaves permit gas exchange. 3. These openings in woody stems are filled with complementary cells from the cork cambium. 4. This mechanism governing gas exchange involves the movement of particles down their concentration gradient. 5. This is the measure of the tendency of water molecules to move from areas with lesser solutes to areas with more solutes. B. Determine the accuracy of each of the following statements. Write true if the statement is correct and false if otherwise. 1. Lenticels do not close, unlike the stomata. 2. Blue light triggers the activation of H+-ATPase. 3. The loss of water in guard cells causes them to open the stoma. 4. The abscisic acid is activated by a decrease in the pH level of guard cells. 5. When guard cells take up water, their turgor pressure decreases. 6. The respiration rate during the day is lower than the photosynthetic rate. 7. During the night, the overall production of oxygen and the consumption of carbon dioxide is relatively higher. 8. When the surface area of a respiratory membrane is smaller, the rate of gas exchange increases. 9. When the plasma membrane of guard cells becomes depolarized, potassium ions move out of the guard cells. 10. The increase K+ concentration in the guard cells leads to the movement of water from the guard cells into the subsidiary cells. 10.1. Gas Exchange in Plants 19 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange C. Determine how plants compensate for the following factors and possible problematic situations to improve the efficiency of their gas exchange. 1. the limited surface area of the leaves 2. slow transport of gases to plant parts 3. all plant cells respire and need oxygen 4. extreme heat causing too much transpiration 5. stomata being light-mediated and close at night Challenge Yourself Answer the following questions concisely. 1. Stomata open during the day to allow for gas exchange and close at night to conserve water. If the gas exchange can take place both day and night, and transpiration occurs mostly during the day when the temperature is relatively higher, then why is it important for plants to open their stomata during the day despite these conditions? 2. Leaves are essential in gas exchange in plants. However, some plants, such as the deciduous trees, shed their leaves as the winter approaches. How do they manage gas exchange and respiration? 3. Although the opening of stomata is essential for the uptake of gases, it also exposes the plants to water loss through transpiration. Plants in deserts are at risk of losing more water because of extreme heat. How do you think do they manage the opening and closing of their stomata? 4. What could possibly happen if the stomata would not close? 5. It is often stated in elementary science books that plants take in carbon dioxide and give out oxygen, when in fact, they also take in oxygen. When discussing respiration, it is often explained that only animals and humans respire and that plants do not. Do you think it is incorrect to not discuss with grade school students that plants also respire and need oxygen? Provide possible implications for your answer. 10.1. Gas Exchange in Plants 20 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange Photo Credits Cuticle of leaf under microscope by Tyanna is licensed under CC BY-SA 4.0 via Wikimedia Commons. Hirschfeldia incana roots2 (14445249597) by Harry Rose from South West Rocks, Australia is licensed under CC BY 2.0 via Wikimedia Commons. Hazel (Corylus avellana) lenticels by Rosser 1954 is licensed under CC BY-SA 4.0 via Wikimedia Commons. Bibliography BBC. “Gas exchange in plants.” Biology (Single Science): Respiration and gas exchange. Accessed 30 June 2020. https://www.bbc.co.uk/bitesize/guides/zxtcwmn/revision/2 Campbell, Neil, and Reece, Jane. Biology: Eight Edition. Berkeley, California: Pearson Education, 2008. CliffNotes. “Mechanisms for Gas Exchange.” Accessed 30 June 2020. https://www.cliffsnotes.com/study-guides/biology/biology/gas-exchange/mechanism s-for-gas-exchange Hoefnagels, Mariëlle. “General Biology: Books I and II.” Reprint Edition of Biology: The Essentials, 2nd Edition. Philippines: Abiva Publishing House, Inc. and McGraw-Hill Education, 2016. Mader, Sylvia S. Concepts of Biology. New York: McGraw-Hill Education, 2014. 10.1. Gas Exchange in Plants 21 Unit 10: Plant and Animal Organ Systems and Processes: Gas Exchange S-cool: The Revision Website. “General Principles for Efficient Gas Exchange.” Biology: Gas Exchange. Accessed 29 June 2020. https://www.s-cool.co.uk/a-level/biology/gas-exchange/revise-it/general-principles-fo r-efficient-gas-exchange Shipunov, Alexey. Introduction to Botany. North Dakota, USA: Minot State University, 2020. Simon, Eric J., Jean L. Dickey, Kelly A. Hogan, Jane B. Reece. 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