CHEMISTRY INVESTIGATORY PROJECT CHOCOLATE ANALYSIS NAME : SARA C SHAIKH CLASS : 12C ROLL NO : 36 DECLARATION I do hereby declare that the projectbeen entitled CHOCOLATE ANALYSIS being submitted is my authentic work in partial fulfillments of the requirements for the CBSE certificate. NAME : SARA C SHAIKH Class : 12 C ROLL NO : 36 ATOMIC ENERGY CENTRAL SCHOOL NO 4 Certificate This is to certify that this project work is submitted by SARA SHAIKH the chemistry department ATOMIC ENERGY CENTRAL SCHOOL NO 4 and was carried out by her under guidance and supervision during the academic year 2023-24 ……………………………… Teacher in-charge …………………………. Examiner’s signature .…………………………... Principal ACKNOWLEDGEMENT In the accomplishment of this project successfully many people who best have owed upon me their blessings and the heart pledged support, this time I am utilizing to thank all the people who have been concerned with this project. Firstly I thank God for making me able to yield within the span of completing this project. Then I would like to thank my chemistry teacher RAJANI BALA MEENA , whose valuable guidance has been what helped me patch this project and make it full proof success. My teacher’s suggestions and instructions have served as the major contributor towards the completion of this project. Also, I would like to thank my parents and friends who have helped me with their valuable suggestions and guidance, which have been helpful in various phases of the completion of this project. Last but not the least, I am grateful to the internet that has helped me with the most bountiful information. INDEX 馃敻 INTRODUCTION 1. Varieties 2. Manufacture 3. History • Good effects • Bad effects 馃敻AIM 馃敻 CHEMICAL REQUIRED 馃敻PROCEDURE FOR ANALYSIS 1. Protein test 2. Fat test 3. Calcium test 4. Iron test 馃敻CONCLUSION 馃敻 BIBLIOGRAPHY INTRODUCTION Chocolates have become one of the most popular flavours in the world of today. They form the basics ingredient in very many pastries and cake. Chocolates can also be used as hot and Cold Beverages. Each manufacture combines secret formulas of the different varieties of the coca sweets to develop exclusive chocolates and try to make the exotic teat. Gifts of chocolates moulded to different shapes have become traditional on certain festivals and occasions. Chocolates are made from the seeds of COCOA trees. Spanish mythology considers these trees were grown in the garden of the PARADISE and believed that the chocolates drink was Divine. The cocoa tree is a tropical plant, sometimes living and producing for more than 200 years. Chocolates are made from the seeds of these trees. There are many varieties cultivated today and this farming is highly profitable VARIETIES There are three basic varieties of cocoa. Criollo, which has the best but the mildest powder; Forastero, which is hardier plant; and Trinitario, which is a natural hybrid of the two mentioned already. Trinitario combines both flavour and hardness. More hybrids are being developed worldwide to improve the quality of the bean, the yield increase and also resist to disease. MANUFACTURE Pod pickers using long handled knives cut the ripe pods which grow on the both branches of the cocoa trees. The pulp and beans are stalked into piles or boxes of large trays. They are covered with banana leaves and left for fermentation over next 7 days. Fermentation happens in a temperature of 120藲F and hence the beans begin to develop the characteristic colour and aroma. After 7 days fermentation beans are transferred to be dried either in the sunlight or artificially lightened rooms. The ultimate brown colours of the beans indicate that they are finished for being processed. Now the good beans are collected for shipping immediately to various manufactures to avoid any damages by heat or moisture. HISTORY The story of the chocolate spans more than 2000 years. Chocolate was first drunk rather than being eaten. Though started in the tropical rainforest of central and South America were cocoa was first grown, the tales of chocolate cultivation now circles the world. CHOCOLATES AND HEALTH Health effect of chocolates refers to the possible beneficial or detrimental, physiological effects of eating chocolates mainly for pleasure. For example, cocoa and chocolates may support cardiovascular health. Other effects under preliminary research includes reduce risk of cancer, coughing and heart disease. One interpretation on the potential health effect of dietary chocolates are may be lower blood pressure improved vascular function and energetic metabolism, and reduced platelets and aggregation and adhesion. Unconstrained consumption of large quantity of any energy-rich food, such as chocolates, without a corresponding increase in activity, increases the risk obesity. Raw chocolates is high in cocoa butter, a fat removed during chocolate refining, then added back in varying proportions during manufacturing. Manufactures may add other fats, sugar and powder milk as well. GOOD EFFECTS Chocolate may be mild stimulant to humans cocoa has antioxidant activity. Antioxidants help to free your body of free radicals which cause oxidative damage to the cell. Small but regular amounts of dark chocolates are associated with lower risk of heartattack. Dark chocolates contain THEOBROMINE, which has been shown to harden tooth enamel. Cocoa percent of at least 74%,significantly improves the blood flow which were tested onsmokers. Some studies have also observed a modest reduction in the blood pressure and flow mediated dilation after consuming dark chocolates daily. Eating dark chocolates may also prevent arteriosclerosis (Harding of the arteritis). Thus the best type of chocolates that is benefit for you is dark chocolates. BAD EFFECTS While chocolates have many good effects on consuming, it also has many negative side effects. It contains too many bad ingredients including, milk fats and saturated fats caffeine, oxalates and stearic acid. And while sugar may give energy, too much of it can cause tooth decay and gum disease if eating without regular and proper teeth brushing. Sugar plays a harmful role in tooth decay by providing bacteria in your mouth with energy. Bacteria begin to multiply faster, and plague begins to grow in size and thickness on your teeth. Bacteria can also use sugar as a glue to cling to your teeth, making it difficult to get rid of just a tooth brush. Dark chocolates contain a higher amount of caffeine than milk chocolates and this can affect your health. Too much caffeine can lead to hypertension anxiety dehydration and inability to concentrate. AIM To find the presence of • Proteins • Fats • Calcium • Iron in chocolates. CHEMICALS REQUIRED 1. Sodium hydroxide (NaOH) 2. Copper sulphate (CuSO4) 3. Sulphuric acid (H2SO4) 4. Ammonium Chloride (NH4Cl) 5. Ammonium Carbonate ((NH4)2CO3) 6. Ammonium Hydroxide (NH4OH) PROCEDURE FOR ANALYSIS Organic and Inorganic tests were done to find the presence of the different minerals in chocolates. Tests for identification of Calcium and Magnesium (Good Substances) and tests for identification of Lead and Nickel (Poisonous Substances) were also done. 1. TEST FOR PROTEIN TEST FOR PROTEIN EXPERIMENT OBSERVATION 5ml of sample of chocolate Appearance of violet is taken in a test tube. coloration is observed. Add 1-2 drops of CuSO4 soution. INFERENCE Presence of PROTEIN in the sample. RESULT All samples studied showed that they DO contain PROTEIN. 2. TEST FOR FAT TEST FOR FAT EXPERIMENT OBSERVATION INFERENCE Take a small sample of chocolate on a piece of filter paper. Fold and unfold the paper to crush the sample and take it over the flame. Appearance of a translucent spot Presence of FAT in the sample. around the sample which became larger on heating was observed. RESULT All samples studied showed that they DO contain FAT. 3. TEST FOR CALCIUM TEST FOR CALCIUM EXPERIMENT OBSERVATION INFERENCE Add a mixture of NH4OH+(NH4)2CO3 to the sample. A white precipitate was obtained. Presence of CALCIUM in the sample. With help of a glass rod Brick red colour flame was the sample of chocolate obtained. solution is placed on the watch glass. Add a drop of con.HCl andmix it well to make a paste. This paste is taken on the tip of a new glass rod and shown to the blue flame of spirit lamp. Presence of CALCIUM in the sample. RESULT The sample studied showed that the DO contain CALCIUM. 4. TEST FOR IRON TEST FOR IRON EXPERIMENT OBSERVATION INFERENCE A mixture of NH4Cl+NH4OH is made. This is added to a sample of chocolate solution taken in a test tube. No brown precipitate was obtained. Absence of IRON in the sample. RESULT All samples studied showed that they DO NOT contain IRON. CONCLUSION Serial No. Substance Present/Absent 1. Proteins Present 2. Fats Present 3. Calcium Present 4. Iron Absent BIBLIOGRAPHY •www.foodhealthinnovation.com •www.todaysdietition.com •www.teagasc.ie/research.com •www.wikipedia.org