Xiamen Zeesan Biotech Co., Ltd Add: 884-1 and 884-2 Lianting Road, (Xiang'An) Industrial Area, Torch High-Tech Zone, Xiamen City, Fujian Province, 361101, P.R. China http://www.zsandx.com P.C:361101 Test for Sanity 2.0 System Name： Score： Section A: Single choice question (10 marks each) 1. Which of the following function can be realized at Sanity 2.0 System? ①Nucleic Acid Extraction ②PCR Setup ③PCR Detection ④Result Analysis A. ①②③ B. ①②④ C. ②③④ D. ①②③④ 2. What should be noticed while preparing the reagents? ①Rod cover position ②Tips quantity ③Reagents position ④Reagents volume A. ①②③ B. ①②④ C. ②③④ D. ①②③④ 3. What are the important details when placing the reagents? ①Place PCR kit first ②Press reagent kits when placing ③The direction of extraction cartridges: lysis well faces front door ④The direction of PCR strip: missing side is on the left A. ①②③ B. ①②④ C. ②③④ D. ①②③④ 4. What is the normal change of the PCR detection kit after test finished? ①Two layers in tube ②The volume of reaction tubes is equal ③Solution is clean A. ①②③ ④Solution has beads contamination B. ①②④ C. ②③④ D. ①②③④ 5. What should feedback when observe the abnormal status of the reagent? ①Picture of the kit A. ①②③ ②The lot number of the kit B. ①② ③Operational details C. ②③ D. ①③ 6. The instrument can process up to_______samples at a time. A. 2 B. 4 C. 8 D. 16 7. What are the requirements for the experiment table to place the instrument? ①With strong electromagnetic field and static electricity ②There should be no vibration or other factors that affect the normal operation of the instrument ③Stable ④Flat Xiamen Zeesan Biotech Co., Ltd Add: 884-1 and 884-2 Lianting Road, (Xiang'An) Industrial Area, Torch High-Tech Zone, Xiamen City, Fujian Province, 361101, P.R. China http://www.zsandx.com P.C:361101 A.①②③④ B.③④ C.①②③ 8. Maintenance is required for every A. 500 B. 1000 D.②③④ runs. C. 1500 D. 2000 Section B: True or false (5 marks each) 1. Flexible loading differs from regular testing because the test reagent is divided into two parts: 24-well PCR tube carrier and single tube test reagent. 2. When loading flexible test reagent tube, the position of the protruding structure of the test reagent tube must not overlap with the fixed structure of the extraction reagent loading hole. 3. Customer can mix different batches reagents. 4. During loading flexible test reagent, extraction strips must be assembled together before loading and placed in sequence if more than one sample to be tested. 5. After experiment test finished, customers should turn off and use absorbent cotton dipped in 75% ethanol to wipe and clean the external shell and touch screen. 6. The upgrade of software is equal to the upgrade of formula. 7. Do not unzip the package when upgrading the formula. 8. Flexible loading carrier is disposable. 9. After test running, costumer should pay attention to the rod cover and tip about whether they are broken or missing. If so, the photo and the lot number of the kit should be recorded and transfer to technical supporter. 10. If observing the magnetic rod stains with magnet beads, customer should take the photo and use 75% ethanol clean it before next use. Section C: Fill in the blanks (2 marks each) Routine operation process (Operation Video) 1. Turn on the sanity 2.0 system. The screen displays “new” and “report”. Take out the and the 2. Click “New” to start a new test. The instrument door is opened automatically, and Xiamen Zeesan Biotech Co., Ltd Add: 884-1 and 884-2 Lianting Road, (Xiang'An) Industrial Area, Torch High-Tech Zone, Xiamen City, Fujian Province, 361101, P.R. China http://www.zsandx.com P.C:361101 the kit carrier is extended simultaneously. 3. Scan the QR code on the extraction kit with the matching . Scan the barcodes on the aluminum foil bag of sars-cov-2 test kit. Confirm whether the “test item” displayed on the screen is correct. 4. Scan (or type in) the barcodes on the sample tube. If there are no sample barcodes, the instrument supports inputting the sample information by manual. 5. Vortex the sample tube for 20 seconds. Remove the Transfer 1000 ul of sample into the 6. Transfer 20 ul of of the extraction kit. of the extraction kit. into the loading well of the extraction kit. Tear the aluminum foil bag seal take out the SARS-CoV-2 RT-PCR tube. 7. Place it on the suitable position of the test kit carrier. Place the on the extraction kit carrier. 8. Then, the interface “reagent info” display “load” to indicate the reagent is in position. Scan (or type in) the operator information. This step is optional. 9. Click “start”, the system will pop up the test information confirmation interface. Click “ok”, the instrument starts the test process automatically. 10. The screen displays “test time”, “ After the test is finished, the screen displays “ ” and “ ” during the test process. ”. 11. Click “Print Report” to print the report. Click “Open Door” and take out the extraction reagents and test reagent. Waste disposal should be according to all applicable laws and regulate Xiamen Zeesan Biotech Co., Ltd Add: 884-1 and 884-2 Lianting Road, (Xiang'An) Industrial Area, Torch High-Tech Zone, Xiamen City, Fujian Province, 361101, P.R. China http://www.zsandx.com P.C:361101 Answer Sheet Section A: Single choice question (10 marks each) 1 2 3 4 5 6 7 8 Section B: True or false (5 marks each) 1 2 3 4 5 6 7 8 Section C: Fill in the blanks (2 marks each) 1. _____________________ 2. _____________________ 3. _____________________ 4. _____________________ 5. _____________________ 6. _____________________ 7. _____________________ 8. _____________________ 9. _____________________ 10. ____________________ 9 10
