Analytical Techniques Infra-red Spectroscopy This is when a sample being analysed is irradiated with electromagnetic waves in the infrared region of the electromagnetic spectrum. The machine used is a spectrophotometer, and it detects the intensity of wavelengths of infra-red that pass through the sample The energy absorbed corresponds to changes in the vibration of bonds, leading to the bond being stretched, bend and twist At a specific frequency, the resonance frequency, the largest vibrations are obtained Each type of vibration will absorb characteristic wavelengths of infra-red radiation We can hence identify the presence (or absence) of different functional groups from the absorbance pattern on an infra-red spectrum