ACTIVITY 1 PEA SOUP PONDS: ALGAE INVESTIGATION & ANALYSIS FOR WATER QUALITY Introduction In this activity, students learn how water can be polluted by algal blooms. They grow algae with different concentrations of fertilizer or nutrients and analyze their results as environmental engineers working to protect a local water resource. Engineering Connection Engineers strive to create situations in which water is usable by humans or other living organisms, including plant and animal life. Algae is one method in which engineers can determine the relative health of a water source. For example, a pond with high concentrations of algae will have low oxygen levels. Engineers would test and monitor this water source to ensure that the algae levels return to normal levels, creating a safe habitat for its consumers. Effective pollution control strategies or water treatment technologies would be employed to return the water to its normal condition. Objectives • Determine the effects of fertilizers on algae growth. • Discuss how algae can become a problem for stream habitats and engineered processes if too much of it grows in a stream or pond. • Mathematically represent algae growth in the form of a graph and chart. • Develop a basic engineering presentation to explain the results of their algae investigation and what course of action could be taken next. Discussion Please present your answers at the Explanatory Notes section of the Personal Activity Sheet. 1. Did different groups have similar findings? 2. Are there any dead algae on the bottom of their jars? If yes, what will eventually happen to the algae? Would this be good or bad for animals living in the water? How would this affect a wastewater treatment plant? 3. Are there any practices that you have seen that could contribute to this problem? Are there actions that you could take that would improve the situation? How might engineers solve this problem? PERSONAL ACTIVITY SHEET Title: PEA SOUP PONDS: ALGAE INVESTIGATION & ANALYSIS FOR WATER QUALITY Date: Novermber 26, 2023 Location of Activity: Legazpi City Time Started: 10:00 AM Time Finished: 11:00 AM Equipment/Instruments/Materials Used: • 4 baby food jars or Petri dishes • Hot tap water that has been aged for one day • 1 eyedropper • Algal culture (from a biological supplier or scraped from a local fish tank) • Commercially packaged plant fertilizer pellets or loose fertilizer (any type of commercial plant food may be substituted) • Artificial light source, preferably fluorescent • 4 2-3" strips of masking tape or wax pencil Group Name: Group KMJS Designation of Members: • Cernechez, Kristine Marie P. • Emraca, Alessandra Nicole • Lavilla, Hazel Ann C. • Nabor, Marjorie • Serafica, Joan • Udaundo, Christine Observations Pea Soup Ponds Activity Algae Growth Chart Instructions: Record algae growth observations below. Fertilizer Concentration Zero Concentration Day 1 Day 2 Day 3 Day 4 Day 5 No noticeable difference in algae growth. There were no observable changes or discernible algal growth. There were no observable changes in algal growth. No noticeable difference in algae growth. No noticeable difference in algae growth. Low Concentration No noticeable difference in algae growth. There were no observable changes or discernible algal growth. Moderate Concentration No noticeable difference in algae growth. There were no observable changes or discernible algal growth. High Concentration No noticeable difference in algae growth. There were no observable changes or discernible algal growth. Fertilizer Concentration Zero Concentration Low Concentration Moderate Concentration High Concentration There are minimal visual difference in water but still, there were no observable changes in algal growth. There are minimal visual difference in water but still, there were no observable changes in algal growth. There are minimal visual difference in water but still, there were no observable changes in algal growth. There are minimal visual difference in water but still, there were no observable changes in algal growth. There are minimal visual difference in water but still, there were no observable changes in algal growth. There are minimal visual difference in water but still, there were no observable changes in algal growth. There are minimal visual difference in water but still, there were no observable changes in algal growth. There are minimal visual difference in water but still, there were no observable changes in algal growth. There are minimal visual difference in water but still, there were no observable changes in algal growth. Growth Compared to Control Jar After One Week Less Same More Despite our expectations, after one week, there were no observable changes or discernible algal growth in any of the jars. In our experiment investigating the impact of fertilizer concentrations on algae growth, an unexpected outcome occurred due to the unintentionally use of dead algae instead of live algae. This unintentional substitution led to a lack of observable changes in algal growth over the one-week period. However, we can still provide a theoretical expected outcome based on the intended conditions. Here’s our anticipated outcome for the impact of fertilizer concentration on algae growth. Fertilizer Concentration Growth Compared to Control Jar After One Week Less Same More Low Concentration -- -- Moderate Concentration -- -- After one week, there would be slightly increased algae growth in this jar compared to the zero concentration jar. After one week, the algae would have more robust development High Concentration -- -- compared to both the zero and low concentration jars. After one week, there would be a dense and extensive algae coverage, surpassing the growth observed In the moderate concentration jar and further outpacing the low and zero concentration jars. Explanatory Notes : In our experiment, our main objective was to investigate how plant fertilizer affects the growth of algae in water. We hypothesized that the nutrients nitrogen and phosphorus, commonly found in plant fertilizers, would enhance the proliferation of algae, as these nutrients are essential elements for algal development. However, we encountered an unforeseen challenge during our experiment. While preparing our samples, we unintentionally made a mistake and used dead algae instead of live ones. This substitution had a significant impact on our results. The deceased state of the algae had a profound effect on the outcome of our study. Since dead organisms cannot utilize nutrients for growth and development, the introduced concentrations of fertilizer became ineffective in stimulating any discernible growth. Consequently, we did not observe any noticeable changes in the algae specimens across all of the jars. This unexpected error taught us a valuable lesson about the importance of precision and accuracy in scientific research. The use of dead algae has underscored the importance of confirming the viability of organisms before initiating experiments. Recommendation/Conclusion: Engineers often look at algae and small organisms to help determine the health of a river, lake or pond. Green algae are often called plants because they are green like plants and carry out photosynthesis, but under most classification schemes, they are neither plants nor animals but are protists — an organism that is microscopic and shares traits with both plants and animals. When dissolved nutrients — such as nitrogen and phosphorus, found in fertilizers and waste products — are added to a lake, algae can reproduce very quickly because they have plenty to “eat.” The lake turns greenish, and the water situation is called an algae bloom. (https://www.teachengineering.org/activities/view/cub_enveng_lesson02_activity1#comments) Our experiment showed that the expected impact of fertilizer on algae growth did not occur due to the unintentionally use of dead algae. The deceased organisms did not respond to the fertilizer concentrations, however, the theoretical expectation remains that live algae would likely grow in response to the nutrient supply. The impact of dead algae on animals living in the water can be both good and bad. Dead algae can lead to a decrease in oxygen levels in the water where it is too low for aquatic animals to survive. In these areas, fish and other organisms may experience suffocation or be forced to migrate. However, dead algae can also provide a food source for certain organisms. Decomposers, such as bacteria and fungi, break down the dead algae, releasing nutrients into the water. This can benefit detritivores and filter-feeding organisms that feed on these decomposers and detritus. Excessive nutrient inputs, improper waste disposal, and deforestation can contribute to the enrichment of nutrients which leads to the problems of excessive algae growth. To improve the situation, agricultural farmers should adopt responsible fertilizer application practices to reduce nutrient runoff from agricultural activities. Disposing of organic waste, such as sewage and agricultural waste in a proper disposal and support conservation efforts can also help mitigate nutrient runoff. On the engineering front, engineers can contribute to solving this problem through various approaches such as implementing effective water management strategies, designing effective wastewater treatment systems, constructing artificial wetlands, and promoting sustainable agriculture practices. These practices can help in reducing the amount of pollutants and excess nutrients entering water bodies. These practices and engineering solutions, can contribute in reducing excessive algae growth and improving water quality. Documentation CONTROL SAMPLE ALGAE WITH LOW CONCENTRATION ALGAE WITH MODERATE CONCENTRATION ALGAE WITH HIGH CONCENTRATION