Example Responses – Paper 6 Cambridge IGCSE™ / IGCSE (9–1) Biology 0610 / 0970 For examination from 2023 © Cambridge University Press & Assessment 2024 v1 Cambridge Assessment International Education is part of Cambridge University Press & Assessment. Cambridge University Press & Assessment is a department of the University of Cambridge. Cambridge University Press & Assessment retains the copyright on all its publications. Registered centres are permitted to copy material from this booklet for their own internal use. However, we cannot give permission to centres to photocopy any material that is acknowledged to a third party even for internal use within a centre. Contents Introduction........................................................................................................................................................................4 Question 1.........................................................................................................................................................................5 Question 2.......................................................................................................................................................................10 Question 3.......................................................................................................................................................................13 Question 4.......................................................................................................................................................................16 Question 5.......................................................................................................................................................................18 Question 6.......................................................................................................................................................................21 Example Responses – Paper 6 Introduction The main aim of this booklet is to exemplify standards for those teaching Cambridge IGCSE / IGCSE (9-1) Biology 0610 / 0970. This booklet contains responses to all questions from June 2023 Paper 61, which have been written by a Cambridge examiner. Responses are accompanied by a brief commentary highlighting common errors and misconceptions where they are relevant. The question papers and mark schemes are available to download from the School Support Hub 0610 / 0970 June 2023 Question Paper 61 0610 / 0970 June 2023 Mark Scheme 61 Past exam resources and other teaching and learning resources are available from the School Support Hub 4 Example Responses – Paper 6 Question 1 3 1 A student investigated the effect of temperature on the rate of respiration in yeast cells. When yeast cells respire they release carbon dioxide gas. The student used this method: Step 1 Fill a syringe with 15 cm3 of yeast suspension. Step 2 Gently lower the syringe into the measuring cylinder, as shown in Fig. 1.1. Step 3 Fill the measuring cylinder with hot water. Ensure that the water level in the measuring cylinder is above the syringe nozzle. bubble of carbon dioxide gas measuring cylinder water syringe nozzle yeast suspension Fig. 1.1 Step 4 Measure the temperature of the water in the measuring cylinder. Step 5 Start the stop-clock and wait for two minutes. Step 6 After two minutes, reset the stop-clock to zero. Step 7 Start the stop-clock again and count the number of bubbles produced by the yeast suspension in three minutes. Step 8 Pour the hot water in the measuring cylinder into the waste container. The syringe containing the yeast suspension should remain in the measuring cylinder. Step 9 Fill the measuring cylinder with cold water. Ensure that the water level in the measuring cylinder is above the syringe nozzle. Step 10 Repeat steps 4 to 7. 5 Example Responses – Paper 6 4 Fig. 1.2 shows the thermometer during step 4 and step 10. 50 50 40 40 30 30 20 20 10 10 0 0 °C °C cold water hot water Fig. 1.2 Fig. 1.3 shows the number of bubbles the student counted in three minutes in step 7 and step 10. hot water – 54 bubbles cold water – 12 bubbles Fig. 1.3 © UCLES 2023 0610/61/M/J/23 6 (a) (i) Prepare a table for the results and record the temperatures shown in Fig. 1.2 and the 5 results shown in Fig. 1.3. (a) (i) Prepare a table for the results and record the temperatures shown in Fig.Responses 1.2 and –the Example Paper 6 results shown in Fig. 1.3. 5 (a) (i) Prepare a table for the results and record the temperatures shown in Fig. 1.2 and the 5 results shown in Fig. 1.3. (a) (i) Prepare a table for the results and record the temperatures shown in Fig. 1.2 and the results shown in Fig. 1.3. temperature / oC number of bubbles 42 54 18 12 [3] Examiner comment • • (ii) State a conclusion Some candidates included units in for the the dataresults. cells, usually ºC, or did not put units for temperature in the headings, or gave ‘bubbles’ rather than ‘number of bubbles’ for the heading. ........................................................................................................................................... Some misread the thermometers in Fig. 1.2, often as 41 ºC and 19 ºC. ........................................................................................................................................... [3] (ii) (iii) (ii) ..................................................................................................................................... [1] State a conclusion for the results. [3] the higher thecalculate temperature greater the rate of respiration Using the results, the rate the of bubble production in bubbles per minute for the ........................................................................................................................................... State conclusioninfor the results. yeast a suspension hot water and in cold water. ........................................................................................................................................... [3] ........................................................................................................................................... ..................................................................................................................................... [1] State a conclusion for the results. ........................................................................................................................................... [3] (iii) Using the results, calculate the rate of bubble production in bubbles per minute for the ........................................................................................................................................... ..................................................................................................................................... [1] Examiner yeast a suspension hot water and in cold water. (ii) comment State conclusioninfor the results. (ii) ........................................................................................................................................... The conclusion to the independent variable to bubble the dependent variable. The aim per of the investigation (iii) needed Using therelate results, calculate the rate of production in bubbles minute for theis ........................................................................................................................................... often stated at theyeast start suspension of the method. was measured by counting bubbles, but the dependent inThe hot dependent water and variable in cold water. [1]the variable itself was..................................................................................................................................... the rate of respiration. Many candidates concluded that the higher the temperature, the higher ........................................................................................................................................... number of bubbles produced, but did not relate this to the rate of respiration. (iii) Using the results, calculate the rate of bubble production in bubbles per minute for the rate of bubble production in hot water ............................... bubbles per minute ..................................................................................................................................... [1] yeast suspension in hot water and in cold water. (iii) of bubble production in cold waterproduction ............................... per minute Using therate results, calculate the rate of bubble in bubblesbubbles per minute for the [1] yeast suspension in hot water and in cold water. © UCLES 2023 0610/61/M/J/23 [Turn over rate of bubble production in hot water ............................... bubbles per minute rate of bubble production in cold water ............................... bubbles per minute 18 rate of bubble production in hot water ............................... bubbles per minute [1] 4 rate of bubble production in cold water ............................... bubbles per minute 0610/61/M/J/23 [Turn over [1] rate of bubble production in hot water ............................... bubbles per minute Examiner comment © UCLES 2023 © UCLES 2023 [Turn over Some candidates divided the number of bubbles by0610/61/M/J/23 2 minutes, or 60 seconds. rate of bubble production in cold water ............................... bubbles per minute rate of bubble production in hot water ............................... bubbles per minute [1] © UCLES 2023 rate of bubble production in cold water ............................... bubbles per minute 0610/61/M/J/23 [Turn over [1] 7 Example Responses – Paper 6 6 (iv) State the independent variable in this investigation. temperature ........................................................................................................................................... ..................................................................................................................................... [1] 6 (v) State two variables that were kept constant in this investigation. Examiner (iv) comment State the independent variable in this investigation. 1 ........................................................................................................................................ • Some candidates gave constant variables such as time, or volume of yeast. ........................................................................................................................................... • A few candidates gave the dependent variable, number of bubbles, instead. ........................................................................................................................................... ..................................................................................................................................... [1] 2 ........................................................................................................................................ (v) State two variables that were kept constant in this investigation. ........................................................................................................................................... volume (15 cm3) of yeast 6 [2] 1 ........................................................................................................................................ (vi) (iv) Suggest the yeastvariable suspension left for two minutes in step 5 before starting to ........................................................................................................................................... State thewhy independent in thiswas investigation. count the number of bubbles. time (3 minutes) for counting bubbles 2........................................................................................................................................... ........................................................................................................................................ ........................................................................................................................................... ........................................................................................................................................... ..................................................................................................................................... [1] ........................................................................................................................................... [2] (v) State two variables that were kept constant in this investigation. ..................................................................................................................................... (vi) Suggest why the yeast suspension was left for two minutes in step 5 before starting [1] to count the number of bubbles. Examiner comment 1 ........................................................................................................................................ (vii) Suggest why counting bubbles is not the most accurate method of determining the rate • Step 3 of the investigation stated that the water level in the measuring cylinder should be above the syringe nozzle, respiration in yeast. ........................................................................................................................................... but did not sayof that the volume of water must be constant. • Answers needed to be as precise as possible. When giving time as a constant variable, the answer needed to refer 2........................................................................................................................................... ........................................................................................................................................ to what the time was for. In this case, the answer needed to give the ‘time for equilibration’ or the ‘time for counting bubbles’ as the answer. (vii) (vi) (vii) ..................................................................................................................................... [1] ........................................................................................................................................... [2] ..................................................................................................................................... [1] Suggest why counting bubbles is not the most accurate method of determining the rate of respiration Suggest why in theyeast. yeast suspension was left for two minutes in step 5 before starting to count the number of bubbles. ........................................................................................................................................... the yeast was left for two minutes to allow the temperature of the ........................................................................................................................................... ........................................................................................................................................... yeast to become the same as the water temperature ........................................................................................................................................... ..................................................................................................................................... [1] ..................................................................................................................................... [1] Suggest why counting bubbles is not the most accurate method of determining the rate Examiner comment of respiration in yeast. Some candidates thought that this waiting time was needed so the yeast could start respiring or start producing bubbles. Yeast will........................................................................................................................................... always be respiring so this is not true. ........................................................................................................................................... ..................................................................................................................................... [1] © UCLES 2023 0610/61/M/J/23 8 ........................................................................................................................................... ........................................................................................................................................... Example Responses – Paper 6 ..................................................................................................................................... [1] (vii) Suggest why counting bubbles is not the most accurate method of determining the rate of respiration in yeast. counting bubbles is not an accurate method of determining the rate ........................................................................................................................................... of respiration as the bubbles are different sizes so contain different ........................................................................................................................................... volumes of gas ..................................................................................................................................... [1] Examiner comment • Answers that referred to the difficulty in counting bubbles were also accepted. • Some candidates referred to human error or subjectivity, but did not link this to errors in counting. 7 (b) Measuring the volume of a gas is more accurate than counting bubbles. Using a gas syringe is one method of collecting a volume of gas. Fig. 1.4 shows part of the apparatus that can be used to measure the volume of a gas by a different method. Complete the diagram in Fig. 1.4 by drawing and labelling the two pieces of apparatus that are missing. delivery tube © UCLES 2023 0610/61/M/J/23 measuring cylinder bung yeast suspension container of water Fig. 1.4 (c) Yeast can respire reducing sugars. 9 [2] yeast suspension Example Responsesyeast – Paper 6 suspension Examiner comment • • • • • • container of water container of water Many candidates did not answer this question. The question asked candidates to show a different method to measure volume of gas apart from use of a gas syringe, but many drew a gas syringe. Fig. 1.3 Many drawings included a thermometer in the container of water. [2] Some candidates included a delivery tube, but some of these were labelled as ‘pipes’ or the tube did not enter the measuring cylinder water. (c) Yeast can under respire reducing sugars. Many drawings did not include a second piece of equipment to measure volume. Describedid thenot method youdrawings. would use to test a substance for the presence of reducing sugars. Some candidates label their Fig. 1.4 [2] ................................................................................................................................................... (c) Yeast can respire reducing sugars. ................................................................................................................................................... Describe the method you would use to test a substance for the presence of reducing sugars. ................................................................................................................................................... Take a sample of the substance and add Benedict’s solution. Heat the ................................................................................................................................................... mixture at 80 ºC in a water bath and look for a colour change. ................................................................................................................................................... ............................................................................................................................................. [2] ................................................................................................................................................... Examiner comment • • The Benedict’s test was well known and most candidates knew that Benedict’s reagent should be heated with the ................................................................................................................................................... sample. Candidates were not expected to give the results of the test, although a large number did. ............................................................................................................................................. [2] © UCLES 2023 © UCLES 2023 0610/61/M/J/23 0610/51/M/J/23 10 [Turn over Example Responses – Paper 6 8 (d) Bread is made from flour, water and yeast which are mixed to form a dough. Fig. 1.5 shows a person making bread. dough Fig. 1.5 The carbon dioxide gas produced by yeast causes the volume of the dough to increase. Sodium chloride (salt) is often added to dough when making bread. The sodium chloride affects the rate at which the yeast respire. Plan an investigation to determine the effect of the mass of sodium chloride on the volume of dough. Make three balls of dough each containing the same volume and type of ................................................................................................................................................... flour, yeast and water. ................................................................................................................................................... Mix a different mass of sodium chloride (1 g, 2 g and 3 g) into each ball ................................................................................................................................................... of dough and knead to combine. Use a balance to measure the mass of ................................................................................................................................................... sodium chloride. ................................................................................................................................................... Measure the initial volume of dough using a measuring cylinder. ................................................................................................................................................... Leave the dough for 30 minutes at 20 ºC. ................................................................................................................................................... After this time measure the final volume of dough, using the measuring ................................................................................................................................................... cylinder and calculate the change in volume. ................................................................................................................................................... Repeat this investigation two more times. ................................................................................................................................................... ................................................................................................................................................... ................................................................................................................................................... ............................................................................................................................................. [6] [Total: 20] © UCLES 2023 0610/61/M/J/23 11 Example Responses – Paper 6 Examiner comment • • • • • Candidates needed to be precise when describing the constant variables. For example, saying ‘the experiment is left for about half an hour’ is not enough. Some candidates did not describe a valid dependent variable. Some described the need to measure how much the dough had risen, but did not state that this could be done by measuring the volume of dough before and after. Some candidates thought the volume of carbon dioxide produced needed to be measured using a gas syringe. Many candidates needed to include more detail in their method. For instance, by describing the use of a balance to measure the mass of the dough ingredients and describing a method to measure the volume of dough, e.g. using a measuring cylinder. For the investigation design question, candidates were awarded a mark for stating that the experiment should be repeated at least two more times. Some candidates did not mention repetitions, or only repeated the experiment once. Some candidates stated the name of the independent variable, but gave no indication as to how this variable would be changed. 12 Example Responses – Paper 6 8 2 Fig. 2.1 is a2photograph of a cross-section of a root from a carrot plant, Daucus carota. Question P 2 10 Q Fig. 2.1 is a photograph of a cross-section of a root from a carrot plant, Daucus carota. P Q magnification ×6 Fig. 2.1 (a) (i) Draw a large diagram of the carrot root cross-section that shows×6 the layers visible in magnification Fig. 2.1. Fig. 2.1 (a) (i) Draw a large diagram of the carrot root cross-section that shows the layers visible in Fig. 2.1. [4] © UCLES 2023 13 0610/51/M/J/23 Example Responses – Paper 6 Examiner comment • • Some candidates included shading or cells in their diagram, so were not awarded the first marking point. Occasionally, candidate drawings were too big and crossed into the text at the top or bottom of the question. 11 (ii) 11 Line PQ on Fig. 2.1 represents the diameter of the carrot root cross-section. (ii) Line PQ on 2.1 of represents theFig. diameter Measure theFig. length line PQ on 2.1. of the carrot root cross-section. Measure the length of line PQ on Fig. 2.1. mm length of PQ ............................................... 109of the carrot root length of PQ mmcross-section using the formula and your Calculate the............................................... actual diameter measurement. Calculate the actual diameter of the carrot root cross-section using the formula and your length of line PQ in Fig. 2.1 measurement. magnification = actual diameter of the carrot root cross-section length of line PQ in Fig. 2.1 magnification Give your answer to=one decimal place. actual diameter of the carrot root cross-section Give your Space for answer working.to one decimal place. Space for working. 109 = 18.1667 6 18.2 ................................................................... mm [3] ................................................................... mm [3] Examiner comment • • The question asked for the answer to be given to one decimal place, but some candidates did not give an answer to one decimal place. Some candidates incorrectly multiplied by 6 rather than dividing by 6 and some had difficulties converting cm to mm. 12 (b) A student investigated the effect of the concentration of a salt solution on the mass of carrot cubes. The student used this method: • • • • • • (i) Carrots were cut into cubes. Each side of the cube was 1 cm in length. The initial mass of each carrot cube was measured and recorded. Each carrot cube was put into a different concentration of salt solution. The carrot cubes were left in the salt solutions for one hour. After one hour, the carrot cubes were removed from the salt solution and dried with a paper towel. The final mass of each carrot cube was measured and recorded. State the dependent variable in the investigation described in 2(b). the dependent variable was the mass of the carrot cubes ........................................................................................................................................... ..................................................................................................................................... [1] (ii) State two variables that were kept constant in this investigation. 14 • • • Each carrot cube was put into a different concentration of salt solution. The carrot cubes were left in the salt solutions for one hour. Exampleand Responses – Paper 6 After one hour, the carrot cubes were removed from the salt solution dried with a paper towel. 12 • The final mass of each carrot cube was measured and recorded. (b) A student investigated the effect of the concentration of a salt solution on the mass of carrot Examiner (i) comment State dependent variable in the investigation described in 2(b). cubes. Thethe student used this method: Common incorrect answers included ‘mass’, which was not enough, or ‘concentration of salt solutions’ which was the •........................................................................................................................................... Carrots were cut into cubes. Each side of the cube was 1 cm in length. independent variable. • The initial mass of each carrot cube was measured and recorded. ..................................................................................................................................... [1] • Each carrot cube was put into a different concentration of salt solution. • The carrot cubes were left in the salt solutions for one hour. (ii) •StateAfter two one variables were kept constant in this investigation. hour, that the carrot cubes 12were removed from the salt solution and dried with a paper towel. the initial volume of the carrot cube •1 ........................................................................................................................................ The final massthe of effect each carrot was measured and recorded. (b) A student investigated of the cube concentration of a salt solution on the mass of carrot cubes. The student used this method: thethetime that variable the carrot were described left in the salt solution 2 ........................................................................................................................................ (i) State dependent in thecubes investigation in 2(b). [2] • Carrots were cut into cubes. Each side of the cube was 1 cm in length. ........................................................................................................................................... • The initial mass of each carrot cube was measured and recorded. Examiner (iii) comment why it was important dry athe carrot cubes before measuring the final mass. •Explain Each carrot cube was puttointo different concentration of salt solution. 3 [1] • The initial volume of thecarrot carrot cubes cube was theleft same for all carrot cubes for (1 cm ). hour. However, the volume of the cubes •..................................................................................................................................... The were in the salt solutions one ........................................................................................................................................... would have changed over the course of the investigation. Therefore, it was not enough to state that the size or • After one hour, the carrot cubes were removed from the salt solution and dried with • • volume of cubes was kept constant. (ii)the State variables that were kept constant in this investigation. atwo paper towel. ........................................................................................................................................... The initial mass notfinal constant, in Tablecube 2.1. was measured and recorded. • was The massas ofshown each carrot 1 ........................................................................................................................................ Some gave ‘time’, unqualified. Descriptions of a variable needed to be sufficiently detailed to be unambiguous and ..................................................................................................................................... [1] awarded (i) a mark. State the dependent variable in the investigation described in 2(b). 2 ........................................................................................................................................ The results of the investigation are shown in Table 2.1. [2] ........................................................................................................................................... (iii) Table 2.1cubes before measuring the final mass. [1] Explain why it was important to dry the carrot ..................................................................................................................................... the extra water on the carrot cube will add to the final mass, so of salt that initial of mass of ........................................................................................................................................... (ii) concentration State two variables were mass kept constant in final this investigation. change in mass / g solution / mol per dm3 carrot cube / g carrot cube / g the cubes should be dried before measuring the final mass 1........................................................................................................................................... ........................................................................................................................................ 0.0 0.97 1.04 0.07 0.2 0.98 0.99 0.01 [1] 2..................................................................................................................................... ........................................................................................................................................ 0.4 0.96 0.90 –0.06 [2] 0.98 –0.12 The results 0.6 of the investigation are shown in Table 2.1. 0.86 Examiner 0.84 –0.15 (iii) comment Explain0.8 why it was important 0.99 to dry the carrot cubes before measuring the final mass. Table 2.1 • Many candidates referred accuracy or reliability. Their refer to how the extra 1.0 to improvements in0.95 0.79 answer needed to–0.16 ........................................................................................................................................... water would have contributed to the final mass of the carrot cube. 1.2 0.96 0.80 –0.16 concentration of salt initial mass ofbe dried tofinal massthe ofexcess salt solution, but did not • Some candidates said that the carrot cubes needed to remove change in mass / g 3 per dm carrot cube / g ........................................................................................................................................... explain thatsolution this extra/ mol solution would addcarrot to the cube mass./ g 0.0 0.97 1.04 0.07 ..................................................................................................................................... [1] 0.2 0.98 0.99 0.01 0.4 0.96 0.90 –0.06 The results of the investigation are shown in Table 2.1. 0.6 0.98 0.86 –0.12 Table 2.1 0.8 0.99 0.84 –0.15 1.0 0.95 0.79 –0.16 concentration of salt initial mass of final mass of change–0.16 in mass / g 1.2 0.96 0.80 © UCLES 2023 solution / mol per dm3 0610/61/M/J/23 carrot cube /g carrot cube / g 0.0 0.2 0.4 0.6 0.8 1.0 1.2 © UCLES 2023 0.97 0.98 0.96 0.98 0.99 0.95 0.96 1.04 0.99 0.90 0.86 0.84 0.79 0.80 0610/61/M/J/23 15 0.07 0.01 –0.06 –0.12 –0.15 –0.16 –0.16 Example Responses – Paper 6 11 (iv) Using the data in Table 2.1, plot a line graph on the grid to show the effect of concentration of salt solution on the change in mass of the carrot cubes. One axis has been started for you. 0.1 0.05 concentration of salt solution / mol per dm3 Using the data in Table 2.1, plot a line graph on the grid to show the effect of concentration 0.0 of salt solution on the change in mass of the carrot cubes. 0.2 0.4 0.6 0.8 1.0 1.1 1.2 13 change in mass / g (iv) One axis has been started for you. -0.05 -0.1 -0.15 0.0 -0.2 [4] Examiner comment • • • • (v) Using your graph, estimate the concentration of salt solution at which there is no change Many candidates made the scale for the y-axis too small, so the data points did not cover at least half the available in the mass of the carrot cube. space. Some candidates gave on the y-axis were your out byestimate. a factor of 10, for example giving 0.5 rather than Show onvalues the graph how youwhich obtained 0.05. Some candidates gave a non-linear scale and some had the axes the wrong way round. The independent ........................................... mol per variable dm3 needed to be on the x-axis. [2] As osmosis experiments involve mass loss and negative values, candidates needed more practice constructing and plotting this information nature would in beTable useful.2.1, calculate the percentage change in mass of the (vi) graphs Usingofthe carrot cube that was placed in the 0.4 mol per dm3 salt solution. (v) [4] Space for working. Using your graph, estimate the concentration of salt solution at which there is no change in the mass of the carrot cube. Show on the graph how you obtained your estimate. (vi) 0.23 ........................................... mol per dm3 [2] ............................................................ % Using the information in Table 2.1, calculate the percentage change in mass of the [2] carrot cube that was placed in the 0.4 mol per dm3 salt solution. 16 Space for working. Example Responses – Paper 6 Examiner comment • • • • • The example response shows the intercept as a cross on the x-axis. This is the ideal answer. However, most graphs were drawn with the x-axis labelled along the bottom of the graph paper. In this case it was expected that a line would be drawn from where the graph crossed the zero change in mass, down to the x-axis. [4] A significant number of intercepts were shown where the mass of carrot cubes became constant, i.e. at 1.1 mol per dm3, rather where their line crossed their at zero change in mass. (v) than Using your graph, estimate thex-axis concentration of salt solution at which there is no change Those candidates who did not answer the previous question were unable to answer this question too. in the mass of the carrot cube. More practice is needed for this skill, as some candidates were unsure as to what they were being asked to do. Show to onfollow the graph how you carefully, obtainedas your estimate. Candidates needed the instructions there were many answers given where there was no indication of an intercept on their graph. ........................................... mol per dm3 [2] (vi) Using the information in Table 2.1, calculate the percentage change in mass of the carrot cube that was placed in the 0.4 mol per dm3 salt solution. Space for working. ( 0.9 0.96) 100 6.25 0.96 © UCLES 2023 Examiner comment 0610/51/M/J/23 -6.25 ............................................................ % [2] [Turn over Some candidates divided -0.06 by 0.9 rather than 0.96 and some divided 0.96 by 0.9. 14 (vii) The student did not repeat the investigation and only collected one set of results. Explain why it is better to collect several sets of results. it is better to collect several sets of results so that anomalous results ........................................................................................................................................... can be identified and then discarded from the results before an ........................................................................................................................................... average is calculated ..................................................................................................................................... [1] [Total: 20] Examiner comment • • Some candidates gave explanations that referred to preventing anomalies, increasing reliability or stated that it was so that an average could be calculated, but these were ignored. Candidates needed to use correct vocabulary when describing anomalies. They referred to ‘abnormal results’, ‘errors’, ‘mistakes’, etc. in their answers, that showed the right understanding, but some candidates had not used the correct term. 17 Cambridge Assessment International Education The Triangle Building, Shaftesbury Road, Cambridge, CB2 8EA, United Kingdom t: +44 1223 553554 e: info@cambridgeinternational.org www.cambridgeinternational.org © Cambridge University Press & Assessment 2024 v1