Libyan International Medical University Faculty Of AMS 2rd year (2020-2021) Block : Renal system Week I Glomerular disease I Dr: Warda Musbah Dr. Warda Musbah 1 Intended learning outcomes 2 By the end of this session you will be able to: Describe the pathogenesis and mechanism of glomerular damage Outline mediators of glomerular damage and recognized the different mechanisms of progression in glomerular disease. List classification of glomerular disease and list causes of nephrotic syndrome. PATHOGENESIS OF GLOMERULAR INJURY 1. Antibody-Mediated Injury In situ immune complex deposition Circulating immune complex deposition 2. Cytotoxic Antibodies 3. Cell-Mediated Immune Injury 4. Activation of Alternative Complement Pathway Antibody-Mediated Injury 1. In Situ immune complex disease Anti-GBM antibody induced nephritis Heymann nephritis Antibodies against planted antigens 1. In Situ immune complex disease Anti-GBM antibody induced nephritis The antibodies are directed against intrinsic fixed antigens that are normal components of (GBM) proper resulting in a diffuse linear pattern of staining for the antibodies by immunofluorescence techniques. This is the model of anti-GBM disease (Goodpasture syndrome) which is caused by antibodies against non-collagenous domain of the alpha 3 chain of collagen type IV. 1. In Situ immune complex disease Heymann nephritis The antibodies are directed against intrinsic fixed antigen called Heymann antigen or ‘megalin’ located on visceral epithelial cells resulting in complement activation and the formation of subepithelial deposits and a granular pattern of staining for the antibodies by immunofluorescence techniques. 1. In Situ immune complex disease Antibodies against planted antigens Antibodies can react with antigens that are not normally present in the glomerulus but are “planted” there. These antigens include cationic molecules like DNA, nuclear proteins, bacterial, viral and parasitic products and drugs. A granular pattern of staining is observed by immunofluorescence techniques. 2. Circulating immune complex disease o The glomerular injury is caused by entrapment of circulating antigen-antibody complex within the glomeruli. o This results in complement activation, leukocytic infiltration and proliferation of glomerular and mesangial cells. o The endogenousantigens include DNA and tumor antigens o the exogenous antigens include infectious products. o Granular deposits along the basement membrane, in the mesangium or both by IHC . Mediators of Immune Injury • The mediators—both cells and molecules—are the usual suspects involved in acute and chronic inflammation Cells: • Neutrophils and monocytes • Macrophages and T lymphocytes • Platelets • Resident glomerular cells Soluble Mediators: • Complement activation • Eicosanoids, nitric oxide, angiotensin, and endothelin • Cytokines particularly IL-1 and TNF • Chemokines(PDGF), (TGF-β), (VEGF). Mechanisms of Progression in Glomerular Diseases The two major histologic characteristics of such progressive renal damage are: Glomerulosclerosis Tubulointerstitial fibrosis. Glomerulosclerosis Sclerosis involving portions of some glomeruli (also referred to as secondary FSGS develops after many types of renal injury and can lead to proteinuria and increasing functional impairment. Glomerular sclerosis may be seen even in cases in which the primary disease was non glomerula. Tubular Injury and Interstitial Fibrosis Tubulointerstitial injury, manifested by tubular damage and interstitial inflammation, is a component of many acute and chronic glomerulonephritides. Tubulointerstitial fibrosis contributes to progression in both immune and nonimmune glomerular diseases, for example, diabetic nephropathy. Indeed, there is often a much better correlation of decline in renal function with the extent of tubulointerstitial damage than with the severity of glomerular injury. Glomerular diseases Glomerular diseases encompass a large and clinically significant group of renal diseases. classify glomerular diseases into 2 broad groups: I. Primary glomerulonephritis in which the glomeruli are the predominant site of involvement. II. Secondary glomerular diseases include certain systemic and hereditary diseases which secondarily affect the glomeruli. Clinical Manifestations of Glomerular Diseases Syndrome Manifestations Nephritic syndrome Hematuria, azotemia, variable proteinuria, oliguria, edema, and hypertension Rapidly progressive glomerulonephritis Acute nephritis, proteinuria, and acute renal failure Nephrotic syndrome >3.5 g/day proteinuria, hypoalbuminemia, hyperlipidemia, lipiduria Chronic kidney disease Azotemia → uremia progressing for months to years Isolated urinary abnormalities Glomerular hematuria and/or subnephrotic proteinuria 15 References • Robbins pathologic basis of diseases , 10th edition, 2020. • Harsh Mohan, textbook of pathology, 7th edition , 2015. 19