STAFISCO LINK TECH. PRODUCTION OF BREAD USING WHEAT AND CASSAVA BLEND FLAVOURED WITH GINGER Abstract The study accessed the physical and sensory properties of bread prepared from wheat and cassava flour blend incorporated with different bread improver. Flours were prepared from wheat grains and cassava blends flavoured with ginger. The cassava flour was use in to substitute 80% of wheat flour. Ascorbic acid, gurar gum, panok and STK royal bread improver were incorporated into the wheat and cassava flour blend at 0.5,1,2and 3% levels, respectively. Bread were prepared from blends containing the imporvers and accessed for the color, physical, and sensory properties. The height, width, length weight, Volume and density of the control breads were significantly (p<0.05) lower than those of the breads containing the improvers. These parameters were not significantly (p<0.05) affected by the level of the improvers, the containing 3% of the improvers had the highest value. The control bread had the lowest yield (123.50%), oven spring (2.79cm) and specific volume (3.14com/g). The bread containing guar gum had the highest yield of 134.66%. The bread containing ascorbic acid had the least yield of 125.75% among the breads containing the improvers. The bread containing panok had the highest oven spring. The specific Volumes of the breads were not significantly different (<0.05). The control had. The lowest L*(lightness). A*(redness) and b*(yellowness) value. STAFISCO LINK TECH. CHAPTER ONE INTRODUCTION Bread is a staple food prepared from dough of flour and water usually by baking. It is a good source of good nutrient such as macronutrients (carbohydrates protein, and fats) and micronutrients (minerals and vitamins) that are essential for human health. Its origin dates back to the Neolithic era and is still one of the consumed and acceptable staple food products in all parts of the world. The word bread is used to describe the whole range of different varieties of bread which may vary in weight, shape, crust hardness, c \rumb cell structure, softness, colour and eating quantity. There are many combination and proportion of types of flour and other ingredients and also different traditional recipe and modes of preparation of bread. Bread may leavened by different processes ranging from the use of naturally occurring microbes (sourdough recipe) to high pressure artificial aeration method during preparation or baking. However, some products are left unleavened either for preference or for traditional or religions reasons. In Nigeria, bread has become the second most widely consumed non-indigenous food products after since. It is consumed extensively in most homes, restaurants and hotels. It has been hitherto produced from wheat as a major raw material. In Nigeria, STAFISCO LINK TECH. wheat production is limited and wheat flour is imported to meet the local flour needs for baking products. Thus, huge amount of foreign exchange is used every year for the importation of wheat. Effort has been made to promote the use of composite flour in which flour from locally grown gaps replace a portion of wheat flour for use in bread, thereby decreasing the demand for imported wheat and helping in producing bread. Composite flour is a mixture of wheat with other materials replacing substantially a portion of wheat flour to form suitable flour for baking purposes. The idea of composite flour began by food and Agricultural organization (FAO) in 1960s to reduce the important dependency of developing countries. (Onyeku et al; 2008, Sibel 2006; Owuamanam, 2007). There is now a substantial quantity of composite bread in the market, such bread requires at least 70% wheat flour to be able to rise because wheat contains gluten. Wheat in conventional flour, rich in gluten, which makes a better preference for bread baking. It is however expensive because it is not grown in Nigeria due to the unfavourable climatic condition using huge foreign exchange (IgbabuBianaDooshima, 2014). The unbridled importation of food by developing countries is detrimental to their local economy and threatens food security. Many developing countries spend large proportion of their foreign exchange earnings on food especially wheat. It is STAFISCO LINK TECH. therefore, of economic importance if wheat importation is reduced by the substitution with other locally available raw materials (Onyeku et al; 2008) such as cassava, maize, potato and other carbohydrate flour. High quantity cassava flour has been identified as a local alternative to substitute part of wheat flour in composite flour in Nigeria due to high production of cassava in Nigeria. In Nigeria, cassava is one of the most important crops in terms of production energy intake and contribution to Agricultural GDP. The possibility of using starchy tubers as part of bread production instead of 100% wheat flour depends on their chemical and physical properties. Those in bakery sector have given reasons for the slow adoption of this technology, including ease of deterioration of cassava bread due to high moisture content and microbial load, fear of the presence of toxic components in cassava and finally bulkiness. Of interest in this project work is the production of bread using wheat and cassava flour blends flavoured with ginger. Ginger, an antioxidant have been chosen in this work to be used an a flavouraing agent. Ginger does not only serve an a flavouring agent but also has health benefit. The new study published in LWT-food science and technology suggests that enriching bread with ginger could boost its antioxidant content without having adverse effect on any functional or sensory properties due to potential health benefits, ginger has gained considerable attention as a botanical dietary supplement STAFISCO LINK TECH. in both U.S and Europe in recent years, especially for the use in treatment of chronic inflammatory conditions. Gingerols are group of phenols found in ginger that are suggested to offer health benefit (Nathan Gray, 2010). 1.2 OBJECTIVE OF THE STUDY – To produce bread using composite flours of wheat and cassava flavored with finger of varying ratios. It is hoped that the result will increase the utilization of cassava flour and will reduce the pressure on wheat flour for the production of bread and other bakery products that are of good and acceptable quality. 1.3 BREAD Bread is an age long product and a staple food prepared principally by baking dough of flour. Bread is a food product that is universally accepted as a very convenient of form food that has desirability to all population both the rich and poor, rural and urban. 1.3.1 Nutritional value of bread Bread supplies a significant portion of the nutrients required for growth, maintenance of health and well-being. It is an excellent source of proteins, vitamins, minerals, fiber and complex carbohydrate. It is also low in fat and cholesterol. Bread STAFISCO LINK TECH. is quite bulky, so it takes longer to digest and therefore more satisfying and less fattening than fats, sugars and alcohols commonly consumed in excess. All bread are nutrition’s and the difference between them in nutritional value are not significant if we eat a balance diet. 1.4 WHEAT AND ITS NUTRITIONAL INFORMATION Wheat flour contains from 65-70% starch, but it’s most important value lies in its 9 -14% protein content. Gliadin and gluten are the principal proteins, constituting approximately 8% of the gluten. Cellulose, fats and sugar total less that 4%. Endosperm: The inner part of the wheat kernel (about 83% of the kernel) consists mostly of starch and it is the source of white flour. Of the nutrients in the whole kernel, the endosperm only contains about 43% of the pantothenic acid, 32% of the riboflavin, 12% of the niacin, 6% of the pyridoxine, 3% of the thiamine and 70-75% of the protein. Bran: Of the nutrients in the whole kernel the bran (in addition to vital unabsorbable fiber or roughage) contains about 86% of the niacin, 73% of the pyridoxine, 50% of the pantothenic acid, 42% of the riboflavin, 33% of the thiamine and 19% of the protein. Germs: Of the nutrients in the whole kernel, the germ contains about 64% of the thiamine, 26% of riboflavin, 21% of the pyridoxine, 7% of the pantothenic acid, 2% of the niacin and 8% of protein. STAFISCO LINK TECH. 1.4.1 Health benefits associated with wheat In the words of Beuchat, L.R. (1999) wheat bring with itself loads of health benefits that can be discussed as follows – Bread, which is made of wheat flour, is extremely nourishing which gives strength and vitality to people with high appetite. It also helps in curing nose bleeding when wheat is dissolved in milk and sugar is consumed by the victim. – It is a rich source of vitamin B and basic amino acids including argentine and lysine. – Wheat also helps in curing constipation and also beneficial for people suffering from cancer. – The whole grain in wheat helps in preventing diabetes as it influences the insulin level through maintaining the body weight. 1.5 NUTRITIONAL VALUE OF CASSAVA FLOUR Cassava is good and cheap source of carbohydrate. After sugarcane, is considered the highest producer of carbohydrate. 1.5.1 Health Benefits of Cassava – Cassava has nearly twice the calories than potatoes, highest for any tropical starch rich tubers and roots. 100g root provides 160 calories. Their calorie mainly comes from sucrose forming the bulk of the sugars in tubers, accounting for more than 69% not the total sugars. STAFISCO LINK TECH. – Cassava is a moderate source of some the valuable B- complex group of vitamins such as folate, thiamin, pyridoxine (vitamin B-6), riboflavin and pantothenic acid. – The root is the chief source of some important minerals like zinc, magnesium, copper, iron and manganese for many inhabitants in the tropical belts. As in other roots and tubers cassava too is free from gluten. Gluten free starch is used in special food preparations for celiac disease patients. STAFISCO LINK TECH. CHAPTERTWO LITERATUREREVIEW 2.1 WHEAT Wheat (Triticumaestivum) belongs to the genus Triticum and family Graminae. It provides the world’s largest source of plant food and protein. The protein content and functionality differ for different wheat types. The flour produced from them is used for different food purposes. It is common to produce flour from hard wheat and cakes flours from soft wheat types. It is harvested at different months of the year in different parts of the world. Various types of wheat include hard red winter, hard red spring, durum, soft red winter, spring or winter white wheat, Manitoba, English soft wheat. It is a temperature crop and thrives well under low temperature thus making its cultivation in Nigeria restricted to the Sudan Savannah and Sahel Zones (Okoh, 1988). 2.1.1 ORIGINAL AND DISTRIBUTION OF WHEAT At the beginning of recorded history, wheat was already an established crop whose origin was unknown (Anon, 1953). There is however some evidence that cultivation of wheat started about 6000 years ago in the Syria-Palestine area and spread to Egypt , India, Russia, Turkey and Central Europe from where it spread to other countries STAFISCO LINK TECH. and continents. According to Shellenberger, (1969) and Olugbemi, (1992) countries that produce wheat today include Russia, Switzerland, the United States of America, Belgium, Canada, Norway, Sweden, South Africa, Peru, Australia, Argentina, Chile, New Zealand and Nigeria. It is a temperate crop and thrives well under low temperature thus making its cultivation in Nigeria restricted to Sudan Savannah and Sahel Zones (Okoh, 1998). There are different types of wheat, these include the hard red spring, hard red spring, hard red winter, durum, soft red and spring/winter wheat. Others include Manitoba and English soft wheat (Nnenne, 1998) Wheat grains are ovoid in shape. The wheat is a seed, which is fitted for reproducing the plant from which it came. The germ is an embryo plant, with a radicle, which can grow into a root system and a plumule, which can develop into stems, leaves and ears. 2.1.2 COMPOSITION OF WHEAT GRAIN Like any seed, the wheat kernel is a complex structure with many individual components. However with respect to processing (i.e., milling) the wheat kernel is divided into three general anatomical regions. The outer protective layers of the kernel are collectively called the bran. The bran comprises about 14% of the kernel, by weight, and is high in fiber and ash (mineral) content. The germ, the embryonic wheat plant, comprises only about 3% of the kernel. Most of the lipids and many of STAFISCO LINK TECH. the essential nutrients in the kernel are concentrated in the germ. The remaining inner portion of the kernel is the starchy or storage endosperm, which provides the energy and protein for the developing wheat plant. It is characterized by its high starch and moderately high protein (i.e., gluten) content. The endosperm constitutes the major portion of all kernels and is the primary constituent of flour. Finally a single, highly specialized layer of endosperm cells forms a border between the starchy endosperm and the bran. This layer, called the aleurone, is usually considered part of the endosperm, but it is biologically much more active and, subsequently, contains high enzyme activity. Because of its composition, activity, and location, it can exert a variety of negative effects on the acceptability of flour. Consequently, it is generally removed as part of the bran during most flour milling operations; in fact, millers consider the aleurone to be part of the bran. 2.1.3 WHEAT MILLING Present-day milling processes begin with the cleaning of kernels. Wheat arriving at a mill is generally mixed with such matter as straw, chaff, pebbles, earth, and seeds of various kinds. Coarse and fine material is removed by passing the wheat through sieves, but seeds and other objects that approximate wheat grains in size must be extracted by special means. Cylinders and disks that have perforations of various sizes not only separate remaining foreign particles but also segregate wheat kernels STAFISCO LINK TECH. by size. Next, the wheat is scoured by being passed through an emery-lined cylinder. Tempering, a process by which the moisture content is adjusted for easiest separation of kernel from husk, is the last step in preparing the grain for grinding. Because of the development of wheat varieties that do not require the heavy friction provided by millstones, most grinding is done in roller mills. Corrugated rollers gradually reduce wheat kernels to powder, effecting separation of kernel and husk. Initial rolling takes place in three to six stages, the last stage of which yields bran, middling’s, and flour. Finished flour consists almost entirely of endosperm, or nutritive tissue. Middlings are composed of fragments of endosperm, fragments of husk, and husk fragments with adhering particles. Bran, the broken husk of the grain, is used as feed for livestock and to provide roughage in some types of breakfast cereals. Between each of the stages of rolling, and following the final stage, the ground product is sifted, a process referred to by millers as bolting. Three types of sifters are in common use: the plan sifter, composed of a series of sieves arranged one above another; the reel, covered with silk bolting cloth or wire of a gauge that retains middling; and the centrifugal, an adaptation of the reel equipped with beaters that hurl the product the length of the reel. The finest grades of flour are obtained from the middlings that are left over from the bolting process. The flour is extracted by a process called purification. A purifying STAFISCO LINK TECH. machine is usually a sieve through which a stream of air is passed, separating the flour particles by passage through the meshes of the sieve and by flotation in air. The grades of flour removed at successive stages of the milling process vary widely. For commercial purposes the milling grades are blended to produce standard grades or special commercial brand. 2.1.4 NUTRITIONAL VALUE OF WHEAT Flour contains from 65 to 70 percent starch, but it’s most important nutrient value lies in its 9 to 14 percent protein content. Gliadin and gluten are the principal proteins, constituting approximately 80 percent of the gluten. Cellulose, fats, and sugar total less than 4 percent. ENDOSPERM: The inner part of the wheat kernel (about 83% of the kernel), consists mostly of starch and is the source of white flour. Of the nutrients in the whole kernel, the endosperm only contains about 43% of the pantothenic acid, 32% of the riboflavin, 12% of the niacin, 6% of the pyridoxine, 3% of the thiamine, and 70-75% of the protein BRAN: Of the nutrients in the whole kernel, the bran (in addition to vital unabsorbable fiber or roughage) contains about: 86% of the niacin, 73% of the pyridoxine, 50% of the pantothenic acid, 42% of the riboflavin, 33% of the thiamine, and 19% of the protein STAFISCO LINK TECH. GERM: Of the nutrients in the whole kernel, the germ contains about: 64% of the thiamine, 26% of the riboflavin, 21% of the pyridoxine, 7% of the pantothenic acid, 2% of the niacin, and 8% of the protein. WHEATGRAIN CLEANING CONDITION TO ABOUT 17%MOISTURE BY SOAKING IN WATER PASS THROUGH ROLLER SIEVE (TO REMOVE BRAN FLAKE AND GERM) PASS THROUGH ROLLER AND SIEVES WHEAT FLOUR FIGURE 1: FLOW CHART FOR THE PRODUCTION OF WHEAT FLOUR Source: Ihekoronye and Ngoddy (1985). 2.2 Botany and Classification of Cassava The origins of cassava (Manihotesculenta) have long been obscure but it is believed to have originated to Brazil and Paraguay. It is grown in the tropical and subtropical STAFISCO LINK TECH. area of the world and the most important staple food which has the ability to grow on marginal lands where cereals and other crops do not grow well. It is a perennial structure with edible root; it belongs to the family Euphorbracea (Onwueme, 1978). The edible parts are the tuberous root and leaves. The tuber (root) is somewhat dark brown in colour and grows up to 2 feet long. Cassava is ranked the sixth most important source of calories in human diet and can tolerate drought and grow well in low nutrient soils. The world output of cassava in 1998 was 162 million tones, out of which Africa produce 88 million tones making her the world leader in cassava. The other 4 major cassava production countries are Brazil, Thailand, Zaire and Indonesia (FAO, 1999). Cassava thrives better in poor soils than any other major food plant. As a result, fertilization is rarely necessary. However, planting cuttings on well drained solid with adequate organic matter can increase yields. Cassava is a heat loving plant that requires a minimum temperate of 80oF to grow. Since many cultivars are drought resistant, cassava can survive even during the dry season when the soil moisture is low, but humidity is high. 2.2.1 Processing of cassava The shelf life of cassava is for some few days. Removing the leaves two weeks before harvest lengthens the shelf life to two weeks. Dipping the roots in paraffin or wax or storing them in plastic bags reduces the incidence of vascular streaking and STAFISCO LINK TECH. extends the shelf life to three or four weeks. Roots can be peeled and frozen. Traditional methods include packing the roots in moist mulch to extend shelf life. The flour can be used for baking breads. Dried roots can be milled into flour. Typically, cassava flour may be used as partial substitute for wheat flour in making bread. Bread made wholly from cassava has been marketed in USA to meet the needs of people with allergies to wheat flour. The fresh cassava roots can be sliced thinly and deep fried to make a product similar to potato chips. They can be cut into larger spear-like pieces and processed into product similar to French fries. Roots can be peeled, grated and washed with water to extract the starch, which can be used to make breads, crackers and pasta. The unpeeled cassava roots can be grated and dried for use as animal feed. The leaves add protein to animal feed. Industrial uses where cassava is used in the processing procedures or manufacture of products include papermaking, textiles, adhesives, high fructose syrup and alcohol. 2.2.2 High quality cassava flour High quality cassava flour is simple unfermented cassava flour. It can be used in the production of noodles, biscuits, and snacks food. Industrial user of cassava flour by substituting cassava flour for wheat in Nigeria has been due to economic factor. As long as wheat was readily available at low cost, it remained the favoured raw materials for bakery in Nigeria. The experience gained in during the period when importation of cereals was ban between1987 and 1990 has led food manufacturers STAFISCO LINK TECH. to more readily cassava flour as cheaper alternative to wheat flour if the processor can provide the necessary quality. Harvest/sorting of cassava roots Peel and wash Grate Mechanical grater Dewater (screw and hydraulic press) Pulverize Sundry in a thin layer Mill finely Cassava flour Package Figure 2: Flow chart for the Production of High quality cassava flour in Nigeria. Source: Onabolu et al., 1998 2.3 Spices Spices are one or other of various strongly flavored aromatic substances of vegetable origin obtained from tropical plants, particularly dried roots, seeds, buds, berries, fruits and bark. STAFISCO LINK TECH. Spices, aromatic flavorings made from parts of plants. They are valued not only for their flavoring agent but also for other preparation like stimulation of appetite by increasing salvation carminative action and preservation and antioxidant action with some food The term spice is usually applied to pungent plant products, especially plants native to tropical Asia and the Moluccas, or Spice Islands, of Indonesia. It frequently also includes herbs, which are the fragrant leaves of herbaceous plants, many of which are native to temperate regions. With few exceptions, the spices and herbs known today were being used early in human history. The discovery of many spices probably predates the earliest civilizations, when primitive humans were attracted to the aromatic effects produced by what are now called essential oils, which are found in various plant parts Besides their long use in preserving foods and enhancing food flavour, spices and herbs played important, sometimes magical, roles in medicine. Before the advent of industrially prepared medicines, herbal remedies were commonly prescribed and were often effective, as some practitioners are now rediscovering. 2.3.1 Nature of spices The great varieties of herb and spice flavors are produced from nearly all parts of plants, from the leaves to the roots. Among those producing fragrant leaves are basil, rosemary, sage, savoury, tarragon, and thyme, all of which are small annual or STAFISCO LINK TECH. perennial plants. Bay leaf, or sweet laurel, used to flavour meats, sauces, and vinegars, comes from a shrub or tree. Spices have been classified into various ways depending on their agronomic, morphological or chemical grounds. A. (i) True as tropical spices: Pepper, ginger, capsicum, turmeric. (ii) Spicy seed: Mustard, celery, fennel, and coriander (iii) Herbs: Sage rosemary mint marjoram (iv) Aromatic vegetable: Onion garlic, Chives B. (i) Major spices: capsicum, pepper, and ginger, formeliccardoman. (ii) Tree spices: Cinnamons, cassia, cloves, Pimento, nut may, mace, star anise. (iii) -Umbelliferous fruit: Aniseed, caraway, celery, commander, cumin and dill fennel. -Other seed: Mustard fenugreek. -Coloured spices: Paprika Saffron, safflower. (iv) Leafy spice: sweet bay, rosemary, sage dime, marjoram, oregano savoury, basilterragon, parsley and mind. C. (i) Pungent spices: capsicum, pepper, ginger. (ii) Aromatic fruits and seeds: Nutmeg, Cardoman, Fenugreek. (iii) Umbelliferous fruit: Arised caraway, Cumin. STAFISCO LINK TECH. (iv) Aromatic banles: Cinnamon and Cassia (v) Phenolic spices: Cloves, Primento (vi) Coloured spices: Paprika, Saffron, Turmeric. Spices have been found to posses preservatives antioxidant, antimicrobial and flavouring properties (Parry, 1969). When natural product was from plant origin, it is considered as spices while when natural product is from plant of temperate climates, it is considered as herb (Aurand et al., 1987). 2.3.2 Forms of Spices Products range from whole and ground spices in many different granulations to extractives such as essential oils, oleoresins, liquid and dry soluble, spray-dried or encapsulated flavour and an infinite variety of bland of these products. Each spice form is prepared by different method. The choice of form of spice to be used is based on the product to be seasoned, the physical and chemical characteristics of each form and other factors such as cost and label declaration (Williams and Brown, 1987). 2.4 GINGER Ginger, an herbaceous perennial ZingiberofficinaleRoscoe belonging to the family Zingiberaceae is grown commercially in most tropical regions (Pieris, 1982). The plant is native to tropical South East Asia from where it was later introduced to Jamaica, Africa and other tropical regions of the world (Pieris, 1982). Several STAFISCO LINK TECH. varieties such as Sidda (a local variety) Chinese, Cochin and Calicut are cultivated in Sri Lanka as a home garden crop, an intercrop under coconut, coffee, cocoa and betel and a monocrop. Its complicated, irregular flowers have one fertile stamen and a usually showy labellum, formed from two or three sterile staminodes. The family is cultivated widely in the tropics for its showy flowers and useful products, derived mostly from the rhizomes. These products include the flavoring ginger; East Indian arrowroot, a food starch; and turmeric, an important ingredient in curry powder. The rhizome, which is valued for its flavour, contains two classes of constituents such as the essential oils and oleoresins (Balladinet al., 1998). The essential oil consists of monoterpenes and sesquiterpenes, which contribute to the characteristic flavour of ginger and the more volatile oleoresin, is responsible for the pungent flavour of ginger, which is also a source of anti-oxidants (Balachandran, 2005). Essential oil and oleoresin are internationally commercialized for use in food and pharmaceutical industries. Moreover, ginger is well known all over the world especially as a remedy for disorders of the gastrointestinal tract such as constipation, dyspepsia, diarrhoea, nausea and vomiting (Ghayur, 2005). Ginger is also recommended by the traditional healers in South Asia for use in cardiopathy, high blood pressure and palpitations and also as a vasodilator (Ghayur, 2005) STAFISCO LINK TECH. 2.4.1 Ginger oleoresin Ginger oleoresin can be extracted from ginger roots using suitable solvent like acetone, alcohol or ethylene dichloride and subsequent removal of solvent gives a pleasant smelling dark brown, viscous liquid. This liquid contains both the essential oil responsible for the aroma of ginger and ginger oil responsible for its pungency. Commercial dried ginger has been reported to contain oleoresins in the yields of 3.5 -10 % (Purseglove, 1981), and the pungent principle accounting to 25 % of the oleoresins. The rhizome, which is valued for its flavour, contains two classes of constituents such as the essential oils and oleoresins (Balladinet al., 1998). The essential oil consists of monoterpenes and sesquiterpenes, which contribute to the characteristic flavour of ginger and the more volatile oleoresin, is responsible for the pungent flavour of ginger, which is also a source of anti-oxidants (Balachandran, 2005). Beside ginger oil, ginger oleoresin contains resins, fat carbohydrate and colouring matter (Connel, 1970). For extraction, the dried rhizomes or chips are ground to a coarse powder (30-40 mesh) and extracted with a suitable solvent like acetone or ethylene dichloride, using cold percolation method (Lewis et al., 1972). The quality of oleoresin obtain using acetone is better than from alcohol. STAFISCO LINK TECH. 2.4.2 Uses Ginger oleoresin is used in the flavouring of all kinds of meat products, pickles, and chutneys. It is used in baked foods such as bread, biscuit and cakes. 2.5 BREAD It is universal food products that provide carbohydrate (starches) which are important sources of energy. An excellent source of vitamins, protein, and carbohydrates, bread has been an essential element of human diets for centuries in all regions. It is a staple food prepared by cooking dough of flour and water and possibly more ingredients. Bread is fundamental formed gluten (Kent, 1983) and it is made by baking dough which has wheat flour, water, yeast and salt as its main ingredient, other ingredients include fat, sugar, cassava flour or other cereals, vitamins, milk an gluten content cause a low level of blood cholesterol to be associated with its consumption. As a result of these advantage, bread is widely used as a snacks, part of the course meal for breakfast. 2.5.1 Function of bread baking ingredient 2.5.1.1 Fats or shortenings Fats such as butter, vegetable oils, lard, or that contained in eggs affects the development of gluten in breads by coating and lubricating the individual strands of protein and also helping hold the structure together. If too much fat is included in STAFISCO LINK TECH. bread dough, the lubrication effect will cause the protein structures to divide. A fat content of approximately 3% by weight is the concentration that will produce the greatest leavening action. In addition to their effects on leavening, fats also serve to tenderize the breads they are used in and also help to keep the bread fresh longer after baking. 2.5.1.2 Yeast Yeast is a leavening agent added to have desirable dough. Most leavened breads are made with yeast, a microscopic organism that feeds on carbohydrates in flour, converting them into alcohol and carbon dioxide in a process called fermentation. Breads made with yeast must be allowed time to rise before baking 2.5.3. Sugar Sugar is broken down by the yeast to form CO2. It gives a slightly sweet taste desired in the product. It contributes to the browning of the crust of the product when baked. It reduces the strength of gluten and creates certain textural effects such as hardness. 2.5.4. Salt Salt acts to improve the flavour, texture and colour of the bread. It stabilizes yeast development and toughens the dough, which results in improvement in texture. The gluten structure is strengthened and prevented from baking during fermentation. STAFISCO LINK TECH. 2.5.4. Water This helps to develop gluten, dissolve sugar and causes chemical leavening compounds to start a reaction. It helps the yeast growth during heating and promotes the gelatinization of starch (Maud, 1990). 2.5.5. Bread improvers Bread improvers are frequently used in the production of commercial breads to reduce the time that the bread takes to rise, and to improve the texture and volume of bread. Chemical substances commonly used as bread improvers include ascorbic acid, hydrochloride, sodium metabisulfate, ammonium chloride, various phosphates, amylase, and protease. STAFISCO LINK TECH. CHAPTER THREE MATERIALS AND METHOD 3.1 Raw Materials The raw materials used include Wheat flour (Nigerian Eagle Flour mills, Ibadan), Shortening (Simas Margarine PT Initiboga. Jakarta, Indoresra), Salt, Sugar (Dangote, Nig. Ltd), EDC 2000 (Alensinloye market in Ibadan), Ginger (Kuto market, Abeokuta). NHexane, baking pans and other apparatus (Dept. of Food science and Technology, UNAAB). Apparatus and Equipments used: Pipette, Measuring cylinder, Knife, Milling machine, Soxhlet apparatus, tray, bowl, Mixer, kneading machine, oven. 3.2 METHODS 3.2.1 Preparation of oleoresin from ginger Soxhlet method of extraction was used to obtain the oleoresin from milled ginger spice. Fresh ginger root was washed, peeled and sliced into small pieces. It was dried in the Cabinet drier to reduce the moisture content to the minimum; thereafter the dried ginger was milled into powder and packed into thimbles. DOWNLOAD FULL PROJCT or Email: stafiscolink@gmail.com STAFISCO LINK TECH.