8 Biotechnology Quarter 4 – Module 2: Applications of Biotechnology in Agriculture DIVISION OF ANGELES CITY Biotechnology– Grade 8 Alternative Delivery Mode Quarter 4 –Module 2: Applications of Biotechnology in Agriculture First Edition, 2020 Republic Act 8293, section 176 states that: No copyright shall subsist in any work of the Government of the Philippines. However, prior approval of the government agency or office wherein the work is created shall be necessary for exploitation of such work for profit. Such agency or office may, among other things, impose as a condition the payment of royalties. Borrowed materials (i.e., songs, stories, poems, pictures, photos, brand names, trademarks, etc.) included in this module are owned by their respective copyright holders. Every effort has been exerted to locate and seek permission to use these materials from their respective copyright owners. The publisher and authors do not represent nor claim ownership over them. Published by the Department of Education Regional Director : May B. Eclar PhD, CESO V OIC Asst. Regional Director : Rhoda T. Razon EdD, CESO V Development Team of the Module Writers: Larissa G. Manalili Editors:Sherilyne L. Reyes, Jennifer M. Praza, Edgardo D. Cortez, Jenny S. Tongol, Edythe Hipolito Reviewers: Gemima A. Estrabillo, Emily F. Sarmiento, Hermes Vargas, Adrian Tamayo, Krislene Ida N. Mercado, Noel S. Reganit Mary Queen Orphilla, Billy Ray B. Manuel, Marvin R. Leano, Gemmarie G. Rivas Illustrator: Arnold Arceo Layout Artist: Maricon H. Rivera Management Team: May B. Eclar PhD, CESO V Rhoda T. Razon EdD, CESO V Ma. Irelyn P. Tamayo PhD, CESE Fernandina P. Otchengco PhD, CESE Librada M. Rubio PhD Ma. Editha R. Caparas EdD Emily F. Sarmiento, PhD Gemima A. Estrabillo, PhD Rochella C. David Printed in the Philippines by ________________________ Department of Education – Region III – Schools Division of Angeles City Office Address: Jesus St., Pulungbulu, Angeles City Telephone: (045) 322-5722; 322-4702 888-0582; 887-6099 E-mail Address: angeles.city@deped.gov.ph 8 Biotechnology Quarter 4 – Module 2: Applications of Biotechnology in Agriculture Introductory Message This Self-Learning Module (SLM) is prepared so that you, our dear learners, can continue your studies and learn while at home. Activities, questions, directions, exercises, and discussions are carefully stated for you to understand each lesson. Each SLM is composed of different parts. Each part shall guide you step-bystep as you discover and understand the lesson prepared for you. Pre-tests are provided to measure your prior knowledge on lessons in each SLM. This will tell you if you need to proceed on completing this module or if you need to ask your facilitator or your teacher’s assistance for better understanding of the lesson. At the end of each module, you need to answer the post-test to self-check your learning. Answer keys are provided for each activity and test. We trust that you will be honest in using these. In addition to the material in the main text, Notes to the Teacher are also provided to our facilitators and parents for strategies and reminders on how they can best help you on your home-based learning. Please use this module with care. Do not put unnecessary marks on any part of this SLM. Use a separate sheet of paper in answering the exercises and tests. And read the instructions carefully before performing each task. If you have any questions in using this SLM or any difficulty in answering the tasks in this module, do not hesitate to consult your teacher or facilitator. Thank you. What I Need to Know This module was designed and written with you in mind. It is here to help you master the steps in Recombinant DNA Technology. The scope of this module permits it to be used in many different learning situations. The language used recognizes the diverse vocabulary level of students. The lessons are arranged to follow the standard sequence of the course. But the order in which you read them can be changed to correspond with the textbook you are now using. The module is about: • Application of Biotechnology in Agriculture At the end of this module learners are expected to: 1. describe the characteristics of Genetically Modified (GM) crops; 2. identify the advantages and disadvantages of Genetically Modified (GM) crop in the field of Agriculture; and 3. explain the importance of Genetically Modified (GM) crop in the field of Agriculture. These objectives are aligned with the learning competency which is to discuss the applications of Biotechnology in Agriculture. 1 What I Know Directions: Read each question carefully. Choose the letter of the best answer. 1. Which among the following genetically modified organisms used Bacillus thuringiensis as a gene donor? a. Bt corn b. FlavrSavr tomato c. Golden Rice d. Humulin 2. Which genetically modified food contains beta carotene, a precursor of vitamin A? a. Bt corn b. FlavrSavr tomato c. Golden Rice d. Humulin 3. Which among the following genetically modified organisms used Narcissus pseudonarcissus as a gene donor? a.Bt corn b. FlavrSavr tomato c. Golden Rice d. Humulin 4. What enzyme is produced by bacterium Erwiniauredovora which enabled betacarotene to be accumulated in the rice endosperm? a. endonucleases b. hydrolases c. oxidoreductases d. phytoenedesaturase 5. Which among the following is the first commercially grown GM crop granted with a license for human consumption? a. Corn b. Eggplant c. Rice d. Tomato 6. Which refers to organisms with foreign genes incorporated into their genome? a. Clone b. GMO c. Replica d. Vectors 2 7. Which of the following is NOT a benefit of a transgenic crop? a. Introduction of toxins into the body b. Resist insect c. Survive drought . d. Yield more food. 8. Which is NOT a modification made with the FlavrSavr tomato? a. Added flavours b. Delay ripening process c. Longer shelf life d. Resistant to insect 9. What is the name of the enzyme used to cut DNA at a specific site? a. DNA helicase b. DNA ligase c. Restriction enzymes d. Restriction sites 10. What makes genetically modified crops different from non-genetically modified crops? a. They have foreignDNA. b. They have fewer calories. c. A bacteria growing inside of them. d. The addition of specific minerals. 3 Lesson 1 Applications of Biotechnology in Agriculture What’s In Directions: Arrange the jumbled letters to form the word about the application of Biotechnology in Health. Use the description given for each word as a clue. 1. METS SLLEC Clue: undifferentiated cells. 2. ENEG YPAREHT Clue: a form of therapy that involves inserting one or more corrective genes into the genetic material of a patient's cells to cure a genetic disease. 3. TLDUA MEST ELLSC Clue: type of stem cells found in small numbers in most adult tissues, such as bone marrow. 4. ICNOYRBME EMST LLECS Clue: type of stem cells thatcome from embryos that are three to five days old. 5. METS ELLSC YPARTHE Clue: a form of therapy that promotes the repair response of diseased, dysfunctional or injured tissue using stem cells. In our previous lesson, we learned about the application of Biotechnology in health. You have learned about stem cells. Stem cells are the body's raw materials where cells from which all other cells with specialized functions are generated. In addition, stem cells become specialized cells with a more specific function, such as blood cells, brain cells, heart muscle cells, or bone cells. 4 What’s New Activity 1 Directions: Choose GM crops from the box below to identify what is being described by each statement. A. GM Tomato B. Bt Corn D. GM Sugar Beet 1. 2. 3. 4. 5. It It It It It C. GM Papaya E. Golden Rice is resistant to corn borer. has delayed ripening and longer shelf life. is resistant to weeds. produces beta carotene. is resistant to infection by ringspot virus. What is It In this lesson, we shall explain the Applications of Biotechnology in agriculture. Particularly, we will discuss the various genetically modified crops. Applications of Biotechnology in Agriculture involve scientific techniques such as Genetically Modified Organisms, Bt Cotton and pest resistant plants. It helps in modifying plants, animals, and microorganisms and improves their agricultural productivity. Biotechnology in Agriculture Applications of Biotechnology in Agriculture include the development of transgenic crops - the placement of genes into plants to give the crop a beneficial trait. Benefits include the improved yield from crops, reduced vulnerability of crops to environmental stresses, increased nutritional qualities of food crops, improved taste, texture, or appearance of food, reduced dependence on fertilizers, pesticides, and other agrochemicals, and production of vaccines.cK-12.org (2019) 5 Genetically Modified Crops Comparison of regular corn and Bt corn Figure 1 Bt Corn was first genetically modified with the insertion of toxin gene from the bacterium Bacillus thuringiensis. When the Bt Corn was commercialized, an increase in production was realized because of its resistance to the attack of the corn borer. BT Cotton Figure 2 BT Cotton, half of the cotton grown in the world hasbeen genetically modified to resist pests and pesticides. GMO cotton was created to be resistant to bollworms.GMO cotton not only provides a reliable source of cotton for the textile industry, but it is also used to make cottonseed oil, which is used in packaged foods and many restaurants for frying. GMO cottonseed meal and hulls are also used in food for animals. Some GM potatoes were developed to resist insect pests and disease. In addition, some GM potato varieties have been developed to resist bruising and browning that can occur when potatoes are packaged, stored, and transported, or even cut in the kitchen. While browning does not change the quality of the potato, it often leads to food being unnecessarily thrown away because people mistakenly believe browned food is spoiled. 6 Comparison of Golden Rice with regular rice Figure 3 Golden rice is the genetically modified rice containing beta carotene, a precursor of vitamin A. Goldenrice was genetically modified to produce almost 20 times the beta-carotene of previous varieties. Golden rice was created by modifying the rice genome to include a gene from the daffodil Narcissus pseudonarcissus that produces an enzyme known as phytoene synthase and a gene from the bacterium Erwiniauredovora that produces an enzyme called phyotenedesaturase. The introduction of these genes enabled beta-carotene, which is converted to vitamin A in the human liver, to accumulate in the rice endosperm—the edible part of the rice plant—thereby increasing the amount of beta-carotene available for vitamin A synthesis in the body. Sugar Beets were engineered to grow faster and to be more resistant to weeds. GM canola is used mostly to make cooking oil and margarine. Canola seed meal can also be used in food for animals. Canola oil is used in many packaged foods to improve food consistency. Most GM canola is resistant to herbicides and helps farmers to more easily control weeds in their fields. GM alfalfa is primarily used to feed cattle—mostly dairy cows. Most GM alfalfa are resistant to herbicides, allowing farmers to spray the crops to protect them against destructive weeds that can reduce alfalfa production and lower the nutritional quality of the hay. GM papaya lines were developed to resist infection bythepapayaringspotvirus. Comparison of Bt eggplant with the regular eggplant Figure 4 The first GM eggplant (Bt eggplant) was pest-resistant eggplant. 7 GM peas were created by inserting kidney beans genes into the peas DNA resulting in the production of a protein with pesticidalability. Flavr Savr Tomatoes Figure 5 FlavrSavr is the first commercially grown GM tomato granted with a license for human consumption. FlavrSavr tomato was designed to withstand the rigors of shipping, one that would last for weeks while regular tomatoes shriveled and rot.FlavrSavr tomatoes would not only stave off rotting but also prevent softening of the tomatoes during ripening, allowing them to be transported hundreds of miles without bruising while retaining their vine-ripe taste.Diaz (2020) GM crops are made through a process known as genetic engineering. Genes of commercial interest are transferred from one organism to another. There are two primary methods currently exist for introducing transgenes into plant genomes.The first involves a device called a ‘gene gun’. The DNA to be introduced into the plant cells is coated into tiny particles of gold or tungsten. These particles are then physically shot onto plant cells and incorporated into the genomic DNA of the recipient plant.Isaaa.org (2020) What are the potential benefits of GM Crops? Genetically modified crops or GM crops are used in the following ways: 1. They are more tolerant to stresses such as drought, cold, and heat. 2. They are pestresistant and therefore less dependent on chemical pesticides.3. Genetically Modified crops help to reduce post-harvest losses.4. They help to increase the mineral usage by plants, thereby preventing early exhaustion of soil fertility.5. Genetically modified crops have enhanced nutritional value. Example – Vitamin A enriched rice. Isaaa.org (2020) What are the potential risks of GM Crops? With every technology, there are potential risks. The potential risks of GM crops include: 1. The danger of unintentionally introducing allergens and other antinutritional factors in foods. 2. The likelihood of transgenes escaping from cultivated crops into wild relatives. 3. The potential for pests to evolve resistance to the toxins produced by GM crops. 4. The risk of these toxins affecting non-target organisms.Isaaa.org (2020 8 What’s More Activity 1 Directions: Write FACT if the statement is correct and BLUFF if incorrect, then change the words/phrases to make the statement correct. 1.Bt Corn was first genetically modified with the insertion a toxin gene from the bacterium Escherichia coli. 2.Golden rice is the genetically modified rice containing beta carotene, a precursor of vitamin C. 3. Sugar Beets were engineered to grow faster and to be more resistant to weeds. 4. Genentech is the first commercially grown GM tomato granted with a license for human consumption. 5. GM papaya lines were developed to resist infection by papaya Meleira virus. 6. GM canola is used mostly to make cooking oil and margarine. 7. GM cotton was created to be resistant to the borer. 8. GM peas were created by inserting kidney beans genes into the peas. 9. Bt Corn is resistant to the attack of cutworm. 10. GM alfalfa is resistant to herbicides, allowing farmers to spray the crops to protect them against destructive weeds. Guide Questions 1. What makes GM tomato different from the regular tomato? 2. How is GM canola different from regular canola? 3. How is GM sugar beet different from regular sugar beet? 9 Activity 2 Direction: Fill in the crossword puzzle below by providing the correct words based on the clues given. Across 3. Resistant to Ringspot virus 4. Resistant to pest particularly borers 5. Resistant to weeds Down 1. Delayed ripening 2. Enriched nutritional value Guide Questions 1. What organisms were used to make Golden Rice? 2. What is the name given to GM tomato? 3. What insect is Bt Corn resistant to? Activity 3 Direction: Put a check ( √ ) mark to all statements that describe the advantages of GM crops and a cross ( x ) mark to all disadvantage. 1.Resistance to diseases 2.Introduction of toxins into the human body 3.Faster crop growth 4.Herbicide-resistant insects and weeds 5.Passing on genes to wild species and interrupting natural processes 6.Increased exposure to vitamins and mineral 7.Pests developing genetic resistance 8.Increased crop yield 9.Crop growth in less suitable habitats 10.Reduced pesticide and herbicide use on GM-resistant plants Guide Questions 1. What does an herbicide-resistant crop mean? 2. What does a pesticide-resistant crop mean? 3. Give one example of pesticide-resistant crop and one example herbicideresistant crop. 10 Activity 4 Directions: Write the modifications made in each genetically modified crop listed in the table below. GM Crop 1. Canola 2. Corn 3. Cotton 4. Rice 5. Alfalfa Modification GM Crop 6. Papaya 7. Potato 8. Peas 9. Sugar Beets 10. Eggplant Modification Guide Questions 1. What bacterium is used to make Bt Corn? 2. What makes GM papaya differs from the regular papaya? 3. What insect is Bt cotton resistant to? Activity 5 Directions: Read and understand the article about Bt Corn. Answer the questions below after reading the article. The Asian corn borer remains to be a major corn pest in the Philippines, reducing unit yields by as much as 27%. Due to the worsening damage by the Asian corn borer, Bt corn, a transgenic corn variety resistant to this pest, was recently introduced in Philippine commercial farms to control the pest and increase yields. After one year of commercial adoption, only about 10, 000 ha have been planted to Bt corn in the Philippines, representing only 1% of the total area planted to yellow corn. The major factors influencing adoption included risk perception, education, training and hired labor. The perceived risks by farmers on the use of Bt corn played a significant role in adoption, particularly for the non-users, while users frequently mentioned the benefits, like resistance to corn borer and high yield. The most important perceived risk factor was the harmful health effects on man and animals. The main sources of information, however, were farmers, friends and the church groups that seemed to emphasize more of the perceived risks rather than the benefits. Unless there is a radical change in the farmers’ perception about Bt corn, greater adoption of the technology may not be expected in the future. Substantial unit yield increases of as much as 37% were realized by the Btcorn farms. This translates to an additional profit of PhP10,132 per hectare with a reduction in insecticide expenditures of 60%. An incremental net income of PhP 1.34 per kilo was gained by Bt corn users, although seed cost was twice that of the ordinary hybrid. Many farmers complained about the exorbitant price of seed and expressed willingness to try the Bt corn if only the price of seed was lower. The adoption of Bt corn showed a significant impact on the farm financial performance as shown by the adoption elasticities that were even higher than those observed in the developed countries. Increasing the probability of adoption by 10% increased net farm income by 4.1%. These results clearly favor the current national policy agenda of increased productivity and income for small corn farmers, but adoption levels need to be increased. The Bt corn technology has also brought about significant welfare gains to corn producers and seed companies, albeit the adoption is still low. To further realize the benefits of Bt corn through higher adoption rates, public support is badly needed in the areas of information dissemination, development of the Bt corn seed 11 market and, more importantly, the government incentives that facilitate farmers’ access to the technology. At present, the availability of Bt corn seeds is still limited and domestic seed production capacity is still low. As the seed market is opened to other entrants, the adoption rate and welfare gains are expected to increase in the future. -- By Yorobe and Quicoy Source:Researchgate.net Guide Questions: 1. What are the major factors influencing the adoption of Bt corn? 2. What is the most important perceived risk factor of Bt corn? 3. Based on the article, how would you describe the availability of Bt seeds in the Philippines? 4. Why are farmers hesitant to use Bt seeds? 5. Why do you think Bt corn is beneficial to farmers? Activity 6 Directions: Read and understand the question below, then answer what is being asked. Question: Is the use of Genetically Modified corn beneficial to feed our world or are they a disaster waiting to happen? Your Claim: Your Evidence: Your Justification of the Evidence: GuideQuestions: 1. What GM crop has enriched Vitamin A content? 2. What makes GM corn beneficial to farmers? 3. Why do GM crops help farmer to have greater yields? Guide Questions Rubric 1 point 2 points 3 points 4 points 5 points Answer is completely incorrect, and no support is provided. Answer is correct but there is no support, OR the answer is incorrect but there is some correct support. Answer is correct and there is some support. Answer is correct and support needs continuity. Answer is correct and the support is fully developed. 12 What I Have Learned Direction: Fill in the blanks to complete the statements. 1. Genetically Modified Organisms are plants, animals, bacteria, or fungi whose ________ have been modified by genetic manipulation. 2. GM crops are ________ and therefore less dependent on chemical pesticides. 3. The first genetically modified crop with the insertion of toxin gene from a bacterium was ________. 4.The genetically modified rice containing beta carotene, a precursor of vitamin A is called ________. 5. The first commercially grown GM tomato granted with a license for human consumption is ________. 6. The GM crop engineered to grow faster and to be more resistant to weeds is ____. 7. GM papaya was developed to resist infection by ________. 8-10. Bacillus thuringiensis was used to make ________ , ________ ,________ What I Can Do Activity 1 Direction: Think of a crop that you want to modify to create your own genetically modified crop, and then complete the data below. Description of the GM crop: Advantages of your GM Crop and Possible Risk to: Society: Environment: Rubric Category Reflective Thinking 4 The idea explains the student’s own thinking and learning processes, as well as implications for future learning. 3 The idea explains the student’s thinking about his/her own learning processes. 13 2 The idea attempts to demonstrate thinking about learning but is vague and/or unclear about the personal 1 The idea does not address the student’s thinking and/or learning. Analysis The idea is an indepth analysis of the learning experience, the value of the derived learning to self or others, and the enhancement of the student’s appreciation for the discipline. Making The idea Connections articulates multiple connections between this learning experience and content from past learning, life experiences and/or future goals The idea is an analysis of the learning experience and the value of the derived learning to self or others The idea articulates connections between this learning experience and content from past learning experiences, and/or future goals learning process.. The idea attempts to analyze the learning experience but the value of the learning to the student or others is vague and/or unclear. The idea attempts to articulate connections between this learning experience and content from past learning experiences, or personal goals, but the connection is vague and/or unclear. The idea does not move beyond a description of the learning experience. The idea does not articulate any connection to other learning or experiences Assessment Direction: Choose the letter of the correct answer. 1. Which genetically modified organism has an enriched nutritional value? a. Bt corn c. Golden Rice b. FlavrSavr tomato d. Humulin 2. Which of the following bacterium was used to make pest-resistant crops? a. Bacillus thuringiensis c. Pseudomonas putida b. Escherichia Coli d. Salmonella typhi 14 3. Which is NOT a benefit of genetically modified crops? a. They improve the nutritional quality of foods. b. They allow farmers to greatly increase the number of crops produced. c. They pass on genes to wild species and interrupting natural processes. d. They cause less damage to the environment than conventional chemicalintensive agriculture. 4. Which of the following describes a genetically modified organism? a. An organism with foreign DNA. b. A genetically identical organism. c. An organism with missing genes. d. An organism with a defective gene. 5. What type of virus is a genetically modified papaya resistant to? a. Adeno virus c. Ebola virus b. Corona Virus d. Ringspot virus For questions 6- 10: Choose A – if the first statement is correct B – if the second statement is correct C – if the 1st and 2nd statements are correct D – if the 1st and 2nd statements are incorrect First Statement Second Statement 6. FlavrSavr is the first commercially grown GM tomato granted with a license for human consumption. Sugar Beets were engineered to grow faster and to be more resistant to weeds. 7.Genetically modified crops are pest-resistant and therefore more dependent on chemical pesticides. 8. Golden rice is the genetically modified rice containing Iron. 9. Soybean is the most cultivated GM crop. 10. Gene gun involves the introduction of plant cells coated onto tiny particles of gold or tungsten. All Genetically modified crops have enhanced nutritional value. Genetically modified peas produce a protein with pesticidalability. The GM tomato was developed to resist infection by theringspot virus. The GM corn was made for delayedripening. 15 Additional Activity Activity 1 Direction: Complete the data below by listing two advantages and disadvantages of genetically modified crops. GM Crops Advantages 1. 2. Disadvantages 1 2. 16 What I Know 1. 2. 3. 4. 5. a c c d d 6. b 7. a 8. d 9. c 10. a 17 What’s In 1. 2. 3. 4. 5. What’s More Activity 1 1.Bt Corn was first genetically modified with the insertion of toxin gene from the bacterium Bacillus thuringiensis. 2. Golden rice is the genetically modified rice containing beta carotene, a precursor of vitamin A. 3. True 4.FlavrSavr is the first commercially grown GM tomato granted with a license for human consumption. 5. GM papaya lines were developed to resist infection by papaya Rings virus. Stem Cell Gene Therapy Adult Stem Cells Embryonic Stem Cells Stem Cells Therapy What’s New 1. B 2. A 3. D 4. E 5. C Activity 2 1. Tomato 2.Rice 3.Papaya 4.Corn 5.Sugar Beets Guide Questions 1. Narcissus pseudonarcissus and Erwiniauredovora. 2. FlavrSavr 3. Corn Borer Activity 3 √- 1,3,8,9,10 X- 2,4,5,6,7 Guide Questions 1. Herbicide-resistant crops can tolerate broadspectrum of herbicides, which kill the surrounding organisms but leaves the cultivated crop intact. 6. True 7. GM cotton was created to be resistant to bollworms. 8. True 9.Bt Corn is resistant to the attack of corn borer. 2. . Pesticide-resistant crops have been genetically modified so they are toxic to certain insects. 3. BT cotton and GM Corn 10. True Guide Questions 1. GM tomato was designed to withstand the rigors of shipping, one that would last for weeks while regular tomatoes shriveled and rot. 2. GM canola is resistant to herbicides. 3. GM Sugar Beets grow faster and to resistant to weeds. Answer Key 18 Activity 4 1. Canola – resistant to herbicides 2. Corn – resistant to the attack of corn borer 3. Cotton – resistant to pest 4. Rice – produces beta carotene 5. Alfalfa – resistant to herbicides 6. Papaya – resistant to infection by ringspot virus 7. Potato – resistant to bruising and browning 8. Peas – production of protein with pesticidal effect 9. Sugar Beet – resistant to weeds 10. Eggplant- pest-resistant What I Have Lear ned 1. Genes 2. Pest-resisitant 3.Bt corn 4. Golden Rice 5.Flavr Savr 6. Sugar Beets 7.Ringspot Virus 8. Bt cotton 9.Bt corn 10.Bt eggplant Guide Questions 1. Bacillus thuringiensis 2. GM papaya are resistant to infection by papaya ringspot virus while regular papaya are prone to ringspot virus. 3. Bollworms Bollworms about What I Can Do Answers may vary Addit ional Act ivit y Answers may vary Activity 5 1. The major factors influencing adoption included risk perception, education, training and hired labor. 2. The most important perceived risk factor was the harmful health effects on man and animals. 3. At present, the availability of Bt corn seeds is still limited and domestic seed production capacity is still low. 4. Many farmers complained exorbitant price of seed. the 5. Substantial unit yield increases of as much as 37% were realized by the Bt corn farms Possible answer: Advantages 1. Genetically Modified crops help to reduce post-harvest losses. 2. GM crops help to increase the mineral usage by plants, thereby preventing early exhaustion of soil fertility. Disadvantages 1. . The potential for pests to evolve resistance to the toxins produced by GM crops. 2. The risk of the toxins affecting non target organisms. Activity 6 Answers may vary Possible answer: Claim: Beneficial to feed the world Evidence: Sufficient supply of corn in the market. Justification of the Evidence:Continuous cultivation of corn by farmers Guide Questions 1. Golden Rice 2. Possible answer: GM crops are more tolerant to stresses such as drought, cold and heat. 3. Possible answer: GM crops are pestresistant and therefore less pest infestation. References Application of Biotechnology in Agriculture https://bit.ly/2NcKCD0 C.K. 12. 2019. Biotechnology and Agriculture https://www.ck12.org/book/ck-12biology-advanced-concepts/section/9.6/ Yorobe, Jose M., and Cesar B. Quicoy. 2006. "Economic impact of Bt corn in the Philippines." Philippine Agricultural Scientist 89, no. 3: 258. Godani, K. n.d. Environmental Biotechnology http://bit.ly/30BMXuu Charles, D. 2012. Farmers Face tough choice on how to fight new strain of weeds https://www.npr.org/sections/thesalt/2012/03/07/147656157/farmersface-tough-choice-on-ways-to-fight-new-strains-of-weeds Plant Products of Biotechnology. 2020. Genetically Modified Crops retrieved from http://bit.ly/3bETZF4 U.S. FDA. 2020. GM Crops, Animal Food and Beyond https://www.fda.gov/food/agricultural-biotechnology/gmo-crops-animalfood-and-beyond Fridovich-Keil, J. L. and Diaz, Julia M. 2020. "Genetically modified organism." Encyclopedia Britannica. https://www.britannica.com/science/geneticallymodified-organism. Philippidis A. 2016. Mistakes Shorten First Approved GMO’s Shelf Life https://www.genengnews.com/insights/mistakes-shorten-first-approvedgmos-shelf-life Transgenic animals and Ethical issues https://bit.ly/30I8Zf7 Photo and Images Used Biotech Info Center. 2016. Comparison of Corn with Disease and Bt Corn https://www.sunstar.com.ph/article/90620/Business/Bt-cornmainstreamed New Delhi. 2015. Bt Cotton Hybrid. http://bit.ly/3qPFh2w UPLB IPB Bt Eggplant Project. 2020. GM insect-resistant Bt eggplant could help Philippines prevent as much as 73% crop losses, cut ‘almost daily’ pesticide use. Retrieved from https://geneticliteracyproject.org/2020/10/05/withoutgm-insect-resistant-bt-eggplant-filipino-farmers-face-51-73-crop-lossesalmost-daily-pesticide-spraying/ Flavr Savr. 2013. Retrieved from http://flavrsavrgmo.blogspot.com/2013/01/flavrsavr-tomatoes.html 18 For inquiries or feedback, please write or call: Department of Education – Region III Schools Division of Angeles City Office Address: Jesus St. Pulungbulu, Angeles City Telefax: (045) 322-5722; 322-4702 888-0582; 887-6099 Email Address: angeles.city@deped.gov.ph