Robert Hatch
QMUL
2023
Methods to study metabolism worksheet
Figure 1. The metabolic pathway for the production of heme
Study the figure above. Which technique discussed in the ‘methods to study metabolism’
lecture would you use to answer the following questions, and why?
1. What are the allosteric regulators of Uroporhyrinogen III synthase?
2. How is Uroporhyrinogen III synthase activity affected by hypoxia?
3. Where does aminolevulinic acid end up in individuals with Uroporhyrinogen III synthase
deficiency?
4. How would a deficiency in Uroporhyrinogen III synthase affect heme concentration?
5. How does high altitude training affect heme production?
An answer sheet will be released in week 5. Once you have self-marked your work, you are
then invited to contact Dr Hatch to discuss your answers.