Chapter 4: Biological Molecules Water Water is a key solvent in our body as it helps metabolic reactions take place in our body. Plasma, the liquid part of blood, contains a lot of water, so substances like glucose can dissolve in it. These dissolved substances are transported around the body. Helps in dissolving enzymes and nutrients in the alimentary canal. Helps get rid of waste products from our bodies. Carbohydrates Substances that include sugars, starch, and cellulose; contain carbon and hydrogen 1 molecule of carbohydrate contains about twice as many hydrogen atoms as carbon or oxygen Simplest carbohydrates: sugars, monosaccharides, or glucose. If two simple sugar molecules join together, a larger molecule called a complex sugar or disaccharide is made. Examples of complex sugars: sucrose and maltose. Polysaccharides: A very large complex sugar The cellulose of plant cell walls is a polysaccharide and so is starch. Animal cells have a polysaccharide called glycogen. Used for: Needed for energy. The carbohydrate that is normally used in respiration is glucose. Plants store carbohydrates as starch while animals do not. Chapter 4: Biological Molecules 1 Testing for carbohydrates: Benedict’s test: We can test for the presence of sugars by adding Benedict’s solution to food and heating it. If the food contains reducing sugar (such as glucose or maltose), then a brick-red color will be produced. The mixture changes gradually from blue, through green, yellow, and orange, and finally brick red. If there is no reducing sugar, then Benedict’s solution remains blue. Adding iodine solution to a sample of the food. If there is starch present, a blueblack color is obtained. If there is no starch, the iodine solution remains orangebrown. Fats and oils Also known as lipids that are insoluble in water Liquid at room temperature (oil). Fats and oil contain carbon, hydrogen, and oxygen. Each molecule contains less oxygen than is found in a carbohydrate molecule Fats are made of fatty acids and glycerol Used for: They are used as energy stores In mammals, some cells underneath the skin, store drops of fats and oils. This fat-containing tissue is both an energy store and a heat-insulating layer. This layer of cells is called adipose tissue. Plants store oil in their seeds. It provides a good store of energy for germination. Testing the presence of alcohol using fats and oils: Emulsion test Fats and oils do not dissolve in water However, they do dissolve in ethanol. We can use this to detect their presence in food Chapter 4: Biological Molecules 2 Step 1: the food is shaken with ethanol, to allow any fats in it to dissolve in the alcohol Step 2: the ethanol is poured into a clean tube containing water. If there are fats in the ethanol, they form tiny droplets in the water, which give it a milky appearance. The mixture of tiny droplets in water is called an emulsion Proteins A substance whose molecules are made of many amino acids linked together Each different protein has a different sequence of amino acids Amino acids are substances with molecules containing carbon, hydrogen, oxygen, and nitrogen There are 20 different types of amino acids Used for: All enzymes are proteins Antibodies, which help to protect the body against pathogens, are proteins Protein is present in haemoglobin Important for forming cell membranes in all organisms In humans, hair and fingernails are made from a protein called keratin Testing for proteins Biuret test: involves mixing the food in water, and then adding dilute copper sulfate solution. Then dilute potassium hydroxide solution is gently added. A purple color indicates that protein is present. If there is no protein, the mixture stays blue. Test for vitamin C DCPIP: a purple liquid that becomes colorless when mixed with vitamin C DNA Stands for deoxyribonucleic acid. Chapter 4: Biological Molecules 3 DNA is the material that makes up our genes and chromosomes. We inherit DNA from our parents DNA is made of smaller molecules called nucleotides. Each nucleotide contains a base. The sequence of bases in the DNA molecule determines the sequence of the amino acids that are used to build a protein Nucleotide: molecules that are linked together into long chains, to make up a DNA molecule. Complementary base pairing: the way in which the bases of the two strands of DNA pair up; A always pairs with T, and C with G. Bases: one of the components of DNA Four bases: A, C, G, and T. Their sequence determines the proteins that are made in a cell Chapter 4: Biological Molecules 4 Carbohydrates Fats Proteins Elements they are made of Carbon, Hydrogen, and Oxygen Carbon, Hydrogen, and Oxygen Carbon, Hydrogen, Oxygen, Nitrogen, and Sulfur Smaller molecules of which they are made Simple sugars (monosaccharides) Fatty acids and glycerol Amino acids Easily available energy (17 kJ/g) Storage of energy (39kJ/g); insulation; making cell membranes Making cells, antibodies, enzymes, and hemoglobin; also used for energy Why do organisms need them Chapter 4: Biological Molecules 5