EXPERENTIAL LEARNING PROGRAMME SEED TESTING, STORAGE,CERTIFICATION AND PROCESSING SAM HIGGINBOTTOM UNIVERSITY OF AGRICULTURE, TECHNOLOGY AND SCIENCES ELP REPORT SEED TESTING, STORAGE,CERTIFICATION AND PROCESSING (MODULE -1) SUBMITTED TO : Dr. Biswaroop Mehera Associate Professor (DEAN, NAI) Dr. Devi Singh Associate Professor (Horticulture) ADVISOR: Dr. Bazil A. Singh Assistant Professor (Genetics & Plant Breeding) SUBMITTED BY : AGRICULTURE STUDENT SERIAL NO PID NO NAME 1 19BSCAGH024 Bonigala Haveela Praise 2 19BSCAGH032 Rokkam Henry Samuel 3 19BSCAGH038 Prateek Joseph Tigga 4 19BSCAGH040 Blesslin J Salom 5 19BSCAGH050 Beyon K Jose 6 19BSCAGH055 Adarsh Robert Bhengra 7 19BSCAGH062 Jibin Sebastian 8 19BSCAGH078 Nikith Soby 9 19BSCAGH089 Anne Sweta Bara 10 19BSCAGH096 Jevin Kennady 11 19BSCAGH114 Abhijeet Gaurav 12 19BSCAGH117 Mahima Trivedi 13 19BSCAGH118 Priya patel 14 19BSCAGH121 Abhishek Anand 15 19BSCAGH126 P. Lakshmi Narayan 16 19BSCAGH127 Ashish Kumar 17 19BSCAGH128 Piyush Patel 18 19BSCAGH148 Shweta Mishra 19 19BSCAGH174 Saurav Kr. Jha 20 19BSCAGH178 Pritam Kumar 21 22 19BSCAGH180 19BSCAGH184 Atithi Kumari Vishal Keshri 23 19BSCAGH257 Md. Wali-ur-Rahman 24 19BSCAGH265 Atul Sharma 25 19BSCAGH276 Saurabh suman 26 19BSCAGH277 R N S Nirmal Kiren 27 19BSCAGH290 Sameer Raj 28 19BSCAGH297 Nagendla Pavan Kumar 29 19BSCAGH303 Keloth Sai Charan 30 19BSCAGH305 Cheppali Harsha Vardhan ACKNOWLEDGEMENT We are extremely grateful to everyone who has contributed to successfully completing this Experiential Learning project. The project would not have been possible without your unwavering support, dedication and guidance. Firstly, we would like to convey our sincere gratitude to my faculty guide, Dr. Bazil A. Singh, for providing me with invaluable insights and assistance at every step of the project. Their valuable inputs, timely feedback and expert guidance were instrumental in shaping our project and making it a great success. We would also like to express our appreciation to seniors and faculty members of the Department of Genetics and Plant Breeding, who have provided me with crucial feedback, suggestions and support throughout the project. We are also indebted READY Programme coordinator and Mentor, Dr. Devi Singh. Our Dean, Dr. Biswarup Mehera and to the staff who have provided me with access to resources and facilities that have proved to be invaluable in the completion of this project. Once again, thank you all for your support and contributions. The completion of this project would not have been possible without you. SEED TESTING/STORAGE/CERTIFICATION AND PROCESSING MODULE IN-CHARGE: DR. BAZIL A. SINGH (DEPARTMENT OF GENETICS AND PLANT BREEDING) GROUP PICTURE SERIAL NO TOPICS 1 SEED TESTING 2 SEED TESTING LABORATORY 3 SEED PROCESSING 4 SEED PROCESSING UNIT 5 SEED STORAGE 6 SEED CERTIFICATION 7 CONCLUSION 8 REFERENCES SEED TESTING • The science of see testing, that is, the science of evaluating the planting value of the seeds. • Seed testing helps to assess the quality attributes of the seed lots which have to be offered for sale and minimizing the risk of planting low quality seeds. • The history of agricultural progress from the early days of man has been the history of seeds of new crops and crop varieties brought under cultivation. • In the early days it was achieved through the cultivation of indigenous but useful plants and those taken through introductions. • Later through the well known techniques of selection, hybridization, mutation, polyploidization and plant biotechnology the scientists made available many new and better varieties. • However, to the farmer all this scientific research would be of little value unless he gets seeds, which are genetically pure, high germination percentage and vigour, high purity, sound health etc., • When the farmers do not get seeds possessing these qualities the yields they obtain may not be as expected. • The pace of progress in production therefore, will largely depend upon the speed with which we are able to multiply and market good quality seeds of high yielding varieties. OBJECTIVES OF SEED TESTING • To determine the seed quality i.e., their sustainability for planting. • To identify seed quality problems and their probable causes. • Supply high quality seeds, means seeds of high yielding varieties, varieties with resistance to diseases and pests. • To increase agricultural production by supply of quality seed. • To assure rapid seed multiplication of desirable varieties. • Timely supply of seeds, i.e well before the sowing season. • Supply of seeds at reasonable prices. • To determine the labelling specifications. • To establish quality and provide a basis for price and consumer discrimination among lots in the market. • To determine the need for drying and processing. SEED TESTING LABORATORY Seed testing laboratories are essential organization on seed certification and seed quality control programmes. The main objective is to serve the producer, the consumer and the seed industry by providing information on seed quality. Seed testing laboratory are established and managed properly so that received sample could be analysed in the least possible time. Test result may cause rejection of poor seed multiplication or low grade seed in a count of law. GENERAL PRINCIPLES A seed Testing Laboratory can be housed as a separate building or it could form part of a larger building housing a department. Working space should be adequate. There should be sufficient space left for any special tests section etc. if the need arises. The physical – infrastructure and facilities should be planned on the basis of average expected work load during the peak season. The kind of test like routine test, varietal purity test etc. to be carried out, must be ascertained in advance so that proper plans can be made. The selection and number of equipments should be adequate to permit efficient handling of work. The decent furnishing, light arrangement and all other necessities should be provided for smooth on going of work. WORKING PROCESS 1. Receipt and registration of seed samples : The samples received in the laboratory should be entered in register or forms and test number is assigned. The information like name of the sender, type of sample kind of tests required, crop variety and class of seed etc. Should be recorded. 2. Moisture Test : After assigning test number sample should be passed on for moisture testing analysis without unnecessary delay. 3. Working sample : working sample is prepared for various tests. 4. Routine Tests : Germination test is carried out. 5. Other Test : Vigour, viability, genetic purity, etc. is done. 6. Reporting of results : Results are reported on printed form, known as seed analysis certificate. The result of seed samples received from seed inspectors communicated within 21days from the date of receipt under the provision of Seed Act, (not later than 30 days in any case.) 7. Storage of Guard sample : Stored for one year, drawn from submitted sample and have equal weightage of working sample. 8. Maintenance of Record : Records are maintained so that any information needed can be traced immediately. EQUIPMENTS USED IN SEED TESTING LABORATORY 1. MECHANICAL SEED DIVIDER/BOERNER DIVIDER It is essentially a hopper, inverted cone and a series of baffles directing the seeds into spouts .It mixes and divides samples completely by gravity. It divides a sample into two approximately equal parts. Sample is mixed and passed through the divider .After mixing ,sample is reduced to its half .The process is continued until a working sample is approximately ,but not less than the minimum weight is obtained. 2. CENTRIFUGAL SEED DIVIDER/GAMET DIVIDER It is suitable for all kinds of seed .It uses centrifugal force for mixing and dividing seeds. 3. ELECTRONIC WEIGHING MACHINE It is used weigh the weight of the seed. In this ,cable is attached to power source and ON button is pressed ,make sure reading is zero before going to measure the sample by pressing TARE button. place the sample on the weighing surface and note the reading. 4. SEED GRINDER It is an instrument to grind the seed. sample is fed into the hopper slowly so that mill does not slow down or become jammed .than the seeds will get ground from size ranging from 0.5 to 0.8 mm that ground seed will then pass through the sieve. 5. PURITY WORK BOARD It is a instrument used to examine each particle individually under magnifying lens in transmitter light ( the criteria used being the external appearance such as shape, size, colour ,surface texture) It is used to separate out impurities such as other crop seeds, weeds and inert matter and impurities is put separately into purity dishes. 6. SEED SCARIFIER This instrument is used to reduce the hard seed content and improve germination for successful stand establishment of these crops. It converts non germinating seeds into germinating seeds. It is based on technique to physically damage the seed coat to reduce hard seed while keeping the seed viable. 7. SEED BLOWER : This machine is used to separate good(heavy) seeds from waste( dust ,husk ,straw, and empty seeds) based on their weight. 8. ELECTRIC MAGNIFIER A magnifying glass (called a hand lens in laboratory contexts) is a convex lens that is used to produce a magnified image of an object. The lens is usually mounted in a frame with a handle . 9. SEED ASPIRATOR It is a machine designed to separate seeds on specific weight in a vertical ZZ shaped column, for seed productions as well as laboratory purposes. Specially developed for gravity separation of small batches with high accuracy. 10. STIRRER AND SHAKER It is piece of laboratory equipment used for mixing liquids and solid for laboratory application. 11. SEED DRYER This is a Vertical Model Dryer in which the surface moisture given to seeds during the seed coating / treatment is removed and seeds are brought to original moisture content level before seed coating/ treatment. No Overheating is done to avoid the germination % loss of seeds. 12. MOISTURE METER It is used to measure the percentage of water in given substance. This information is used to determine whether the material is ready for unexpectedly wet or dry or otherwise in need of further inspection. 13. SEED SIEVE It is used for cleaning seeds and screening compost. It is a hand held sieves for heirloom seeds sorting and sorting soils to different particles size in field or laboratory. Its is made of stainless steel with 4 different nets size for easy clean ups. 14. MECHANICAL SIEVE SHAKER It is used for the analysis of size distribution of seeds of the crops by passing it through a series of sieves. 15. AUTOCLAVE Autoclaves operate at high temperature and pressure in order to kill microorganisms and spores. They are used to decontaminate certain biological waste and sterilize media, instruments and lab ware. 16. INCUBATOR Incubator is an insulated and enclosed device that provides an optimal condition of temperature, humidity, and other environmental conditions required for the growth of organisms. An incubator is a piece of vital laboratory equipment necessary for the cultivation of microorganisms under artificial conditions. 17. PH METER pH meter, electric device used to measure hydrogen-ion activity (acidity or alkalinity) in solution. Fundamentally, a pH meter consists of a voltmeter attached to a pHresponsive electrode and a reference (unvarying) electrode. The pH-responsive electrode is usually glass, and the reference is usually a silver-silver chloride electrode, although a mercury–mercurous chloride (calomel). 18. SEED COUNTER It is used for counting the number of seeds in any sample. 19. STEREO MICROSCOPE It is used for low-magnification applications, allowing high-quality, 3D observation of subjects that are normally visible to the naked eye. In life science stereo microscope applications, this could involve the observation of insects or plant life. 20. GERMINATION CHAMBER It is a closed chamber that provides an optimum humidity and temperature control to help start seedlings. Providing the optimum growing environment can decrease germination times and improve germination rate. GERMINATION TESTS Seed germination test Germination is defined as the emergence and development from the seed embryo, of those essential structures, for the kind of seed in question, indicates its ability to produce a normal plant under favourable conditions. Principles Germination tests shall be conducted with a pure seed fraction. A minimum of 400 seeds are required in four replicates of 100 seeds each or 8 replicates of 50 seeds each or 16 replicates of 25 seeds each depending on the size of seed and size of containers of substrate. The test is conducted under favourable conditions of moisture, temperature, suitable substratum and light if necessary. No pretreatment to the seed is given except for those recommended by ISTA. Fig: Germination tests MATERIALS REQUIRED Substratum The substratum serves as moisture reservoir and provides a surface or medium for which the seeds can germinate and the seedlings grow. The commonly used substrate are sand, germination paper and soil. 1. Sand Size of sand particle Sand particles should not be too large or too small. The sand particles should pass through 0.80 mm sieve and retained by 0.05 mm sieve. Toxicity Sand should not have any toxic material or any pathogen. If there is presence of any pathogen found then the sand should be sterilized in an autoclave. 2. Germination tray When we use the sand, germination trays are used to carry out the test. The normal size of the tray is 22.5 x 22.5 x 4 cm. The tray may either zinc or stainless steel. METHOD OF SEED PLACEMENT 1. SAND Methods Seed in sand(S) : Seeds are planted in a uniform layer of moist sand and then covered to a depth of 1 to 2 cm with sand. Top of sand (TS) : Seeds are pressed in to the surface of the sand. Spacing We must give equal spacing on all sides to facilitate normal growth of seedling and to avoid entangling of seed and spread of disease. Spacing should be 1-5 times the width or diameter of the seed. Water The amount of water to be added to the sand will depend on size of the seed. For cereals, except maize, the sand can be moistened to 50% of its water holding capacity. For large seeded legumes and maize sand is moistened to 60% water holding capacity. Fig. Placing the seeds on sand 2. PAPER Most widely used paper substrates are filter paper, blotter or towel (kraft paper). It should have capillary movement of water, at vertical direction (30 mm rise / min.). It should be free from toxic substances and free from fungi or bacteria. It should hold sufficient moisture during the period of test. The texture should be such that the roots of germinating seedlings will grow on and not into the paper. Methods Top of paper (TP) Seeds are placed on one or more layers of moist filter paper or blotter paper in petri plates. These petri plates are covered with lid and placed inside the germination cabinet. This is suitable for those seeds which require light. Between paper (BP) The seeds are germinated between two layers of paper. The seeds are placed between two layers of paper and rolled in towels. The rolled towels are placed in the germinator in an upright position. Fig Sowing of seed in sandy soil EVALUATION OF GERMINATION TEST The germination test is evaluated as Normal seedlings Abnormal seedlings Hard seeds Fresh and ungerminated seeds Dead seeds NORMAL SEEDLINGS Seedlings which has the capacity for continued development into normal plant when grown in favourable conditions of soil, water, temperature and light. Characters of normal seedlings A well developed root system with primary root except in certain species of graminae which normally produce seminal root or secondary root. A well developed shoot axis consisting of elongated hypocotyls in seedlings of epigeal germination. A well developed epicotyl in seedlings of hypogeal germination. One cotyledon in monocotyledon and two in dicotyledons. A well developed coleoptiles in graminae containing a green leaf. A well developed plumule in dicotyledons. Fig: Normal Seedling ABNORMAL SEEDLINGS Seedlings which do not show the capacity for continued development into normal plant when grown in favourable condition of soil, water, temperature and light. Damaged seedlings: Seedligs with any one of the essential structures missing or badly damaged so that the balanced growth is not expected. Deformed seedlings : Weak or unbalanced development of essential structures such as spirally twisted or stunted plumule or hypocotyls or epicotyls, swollen shoot, stunted roots etc. Decayed seedlings: Seedlings with any one of the essential structures showing diseased or decayed symptoms as a result of primary infection from the seed which prevents the development of the seedlings. Damaged Seedling Deformed Seedling Decayed Seedling HARD SEEDS Seeds which do not absorb moisture till the end of the test period and remain hard (e.g.) seed of leguminaceae and malvaceae. FRESH AND UNGERMINATED SEEDS Seeds which are neither hard nor have germinated but remain firm and apparently viable at the end of the test period. DEAD SEEDS Seeds at the end of the test period are neither hard or nor fresh or have produced any part of a seedling. Often dead seeds collapse and milky paste comes out when pressed at the end of the test. Fig: Hard Seed Fig: Dead Seed SEED PROCESSING Seed processing is a vital part of the total technology involved in making available high quality seed. It assures the end users, seeds of high quality with minimum adulteration. In Agriculture, the term seed processing includes cleaning, drying, seed treatment, packaging and storage. Seed processing may be understood to ‘comprise all the operations after harvest that aim at maximizing seed viability, vigour and health. OBJECTIVES Processing of seeds is carried out in approved (By Director of Seed Certification) seed processing plants. Seed processing is to narrow down the level of heterogeneity of the seed lot by using suitable processing methods. Fig: Seed processing unit SEED PROCESSING PROCEDURE Sequences of operations are based on characteristics of seed such as shape, size, weight, length, surface structure, colour and moisture content. Because each crop seed possesses individually seed structure. Therefore, sequence of operation will be applied using proper equipments. However, sequences of operation in seed processing are drying, receiving, pre-cleaning, conditioning, cleaning, separating or upgrading, treating (Drying), weighting, bagging and storage or shipping DRYING Reduction in seed moisture content to a safer limit is called seed drying Its objective is to maintain seed viability and seed vigour for longer period of time during storage If moisture contents are high, higher chances of insect pests attack and deteriorate quality of seed due to higher respiratory activities Natural Drying :Seeds are spread uniformly at neat and clean place and dry under natural conditions i.e under sun Artificial Drying :Different artificial driers are used to dry seeds . • Bag Driers • Box Driers • Bin Driers • Continuous Flow Tower PRE-CLEANING AND CONDITIONING This is the operation that prepares a seed lot for basic cleaning. The equipment required for preconditioning is generally specific for individual crop. Some important preconditioning equipment are: • Sheller • Huller • Debearder BASIC CLEANING This step of seed processing removes the larger, smaller, and thicker adulterants as compares to the crop seed from the seed lots. Basic cleaning is done based on weight, size and density using cleaner with air screen. This process involves following equipment: Grader Scalper Aspirator SEED GRADING Classification of seed lot based on commercial usage viz., size shape, density and colour is known as grading. It is done further improvement of seed lots as finishing operation. It improves the seed lot by removal of cracked, damaged, shriveled and other defective seeds apart from inert matter. SEED TREATMENT Before bagging, seeds are treated with suitable fungicide often in combination with an insecticides. The treatment may be in the form of a powder, liquid, or slurry. Special machines are available for applying each type of treatment. If it is desired not to treat the seed, it goes directly to the bagging machine. BAGGING AND LABELLING After Seed Treatment seeds are distributed in bags of appropriate size (generally 40 kg bags are used), the process is known as bagging and is placed in a paper, plastic, or jute bag. Each bag is labelled with an appropriate label that carries following information: Kind of Seed, Name of Variety, Pure Seed %, Germination %, Date of Testing, Weed Seed %, Inert Matter %, Name and Address of Seller, Period of Validity of Certification and Any other information patent to the Seed Fig: Label of seeds SEED PROCESSING UNIT 1. PRE CLEANER MODEL:- SPC-5T • Cleaning of all types of seeds, grain, pulses and oilseeds. • Dust and light impurities are separated. Fig: Pre Cleaner 2. SEED GRADER Model :DIAMOND (Dx) • Seed cleaning and separation of unwanted seed & inert material. • Separate on the basis of size and shape i.e broken, undersized & irregular shape grain separated. Fig: Seed grader 3. INDENTED CYLINDER MODEL: 1CG -1C23 • Separate on the basis of length of the seed. • Mainly used for paddy. • Remove broken seed i.e. not fully length seed. Fig: Indented cylinder 4. GRAVITY SEPERATOR • Very high grading quality. • Seed of same size & general shape can be often be separated because they differ in specific gravity. • The difference is very useful in removing light immature seed or heavy seed & rock to improve purity, germination of seed. Fig: Gravity seperator 5. ELEVATOR • Used for conveying seed from one machine to other Fig: Elevator 6. TREATER & COATER • Used for dry, liquid & slurry treatment of seed. • Coater is used for liquid chemical film coating of seed. Fig: Treater and coater SEED STORAGE Seed storage is preservation of seed with initial quality until it is needed for planting. The ability of seed to tolerate moisture loss allows the seed to maintain the viability in dry state. Storage starts in the mother plant itself when it attains physiological maturity. After harvesting the seeds are either stored in ware houses or in transit or in retail shops. Storage is needed to maintain the seed in good physical and physiological condition from the time they are harvested until the time they are planted. STAGES OF SEED STORAGE The seeds are considered to be in storage from the moment they reach physiological maturity until they germinate or until they are thrown away because they are dead or otherwise worthless. The entire storage period can be conveniently divided into following stages. Storage on plants ( physiological maturity until harvest). Harvest, until processed and stored in a warehouse. In - storage ( warehouses) In transit ( Railway wagons, trucks, carts, railway sheds etc.). In retail stores. On the user's farm. TYPES OF STORAGE 1. Storage at ambient temperature and humidity Seeds can be stored in piles, single layers, sacks or open containers, under shelter against rain, well ventilated and protected from rodents and store at least for several months. Fig. Seed Storage 2. Dry storage with control of moisture content but not temperature Orthodox seeds will retain viability longer, when dried to low moisture content (4¬8%) and then stored in a sealed container or in a room in which humidity is controlled, than when stored in equilibrium with ambient air humidity. Cool condition is especially favourable. 3. Dry storage with control of both moisture content and temperature This is recommended for many orthodox species which have periodicity of seeding but which are planted annually in large scale afforestation projects. A combination of 4-8% moisture content and 0 to 5° temperature will maintain viability for 5 years or more. Fig. Glimpses of seed storage unit 4. Dry storage for long-term gene conservation Long-term conservation of gene resources of orthodox agricultural seeds is -18°C temperature and 5±11% moisture content 5. Moist storage without control of moisture content of temperature Suitable for storage of recalcitrant seeds, for a few months over winter. Seeds may be stored in heaps on the ground, in shallow pits, in well drained soils or in layers in well ventilated sheds, often covered or mixed with leaves, moist sand, peat or other porous materials. The aim is to maintain moist and cool conditions, with good aeration to avoid overheating which may result from the relatively high rates of respiration associated with moist storage. This may be accomplished by regular turning of the heaps. 6. Moist cold storage, with control of temperature This method implies controlled low temperature just above freezing or less commonly, just below freezing. Moisture can be controlled within approximate limits by adding moist media e.g., sand, peat or a mixture of both to the seed, in proportions of one part media to 1 part seed by volume, and re-moistening periodically or more accurately by controlling the relative humidity of the store. 7. Cryopreservation Seeds are placed in liquid nitrogen at -196°C. Seeds are actually placed into the gaseous phase of the liquid nitrogen -150°C for easy handling and safety. Metabolic reactions come to a virtual standstill at the temperature of liquid nitrogen and the cells will remain in an unaltered state until the tissues are removed from the liquid nitrogen and defrosted. Therefore, little detrimental physiological activity takes place at these temperatures, which prolongs the storage life of seeds. MAINTENANCE OF VIABILITY IN STORAGE Store well mature seeds Seeds should be free from mechanical injury Seeds should be free from storage fungi or micro organisms. Seeds should not have met with adverse conditions during maturation. Storage godown should b e fumigated to control storage insects, periodically Storage environment or godown should be dry and cool. Seeds should be dried to optimum moisture content Required R.H. and temperature should be maintained during storage. Fig: Plastic storage Seeds should be treated with fungicides before storage Suitable packaging materials should be used for packing. HARRINGTON THUMB RULE ON SEED STORAGE The following thumb rules by Harrington are useful measures for assessing the effect of moisture and temperature on seed storage. These rules are as follows: For every decrease of 1% seed moisture content, the life of the seed doubles. This rule is applicable when moisture content between 5 and 14%. For every decrease of 5°C (10°F) in storage temperature the life of the seed doubles. This rule applies between 0°C to 50°C. Good seed storage is achieved when the % of relative humidity in storage environment and the storage temperature in degrees. STORAGE CRITERIA PADDY For short term storage (9-12 months), store the seeds with 12-13% moisture content in gunny bag / cloth bag. For medium term storage (12-36 months), store the seed in HDPE bag or poly lined gunny bag with 10-12% seed moisture SOYBEAN Store the seeds in gunny or cloth bags for short term storage (8-9 months) with seed moisture content of 10-12%. Store the seeds in poly lined gunny bag for medium term storage (12- 15 months) with seed moisture content of 8 – 10%. Store the seeds in 700 gauge polythene bag for long term storage (more than15 months) with seed moisture content of less than 7% SEED CERTIFICATION A legally sanctioned system to maintain quality to seeds during seed production, post harvest operation and distribution of seeds. Seed certification is a quality assurance process. Seeds intended for domestic and international markets is controlled and inspected by official sources in order to guarantee consistent high quality for consumers. It includes field inspection, seed quality tests and pre & post quality check. Certification is a voluntary process but labelling is compulsory. PRINCIPLES • It should have autonomy. • Seed certification procedure adopted should be uniform throughout the country. • It should closely be associated with technical institutes. • It should operate on a no profit and no loss basis. • It should have adequate technical staff and facilities for timely inspection of seed fields. • It should serve for the interests of seed producers and buyers. OBJECTIVES • To maintain and male available to the farmers, high quality seeds and propagating materials of notified kind and varieties. • To ensure the acceptable standards of seed viability, vigour, purity and seed health. • To ensure genetic identity and genetic purity. • A well organized seed certification should help in accomplishing the following 3 primary objectives. • The identification of new varieties and their and their rapid increase under appropriate and generally accepted names. CLASSES OF SEED SEED CLASS TAG COLOUR GENETIC PURITY NUCLEUS SEED - 100% BREEDER SEED GOLDEN YELLOW 100% FOUNDATION SEED WHITE 99.5% CERTIFIED SEED AZURE BLUE 99 % PHASES OF CERTIFICATION APPLICATION • Seed grower can apply online through http://ossopca.nic.in VERIFICATION • SCO(Seed Certification Officer)can do theverticationor forwardtoASCOfor verification as well as inspection. INSPECTION • ASCO or in certain case SCO himself can do the verification • Both online as well as offline module has been developed. THRESHING CERTIFICATE ISSUE • TC is issued only by ASCO/SCO who did the inspection. • Processing plant can now be changed by ASCO/SCO. SAMPLING SLIP GENERATION • Three sample slips are generated before going to do sampling by concerned ASCO/SCO. TESTING LAB • Seed Testing lab will submit their report. TAG CERTIFICATE ISSUE • TAGs are issued by the appropriate authority. CONCLUSION Seed testing is required to assess the seed quality attributes of the seed lots which have to be offered for sale. Testing of seed to evaluate the planting value and the authenticity of the certified lot. Use of quality seeds alone could increase productivity by 15 – 20 % indicate the critical role of seed in agriculture. Seed storage is the maintenance of high seed germination and vigour form harvest until planting The purpose of seed storage is to maintain the seed in good physical and physiological condition from the time they are harvested until the time they are planted. Seed processing is necessary in order to dry the seeds to safe moisture level; remove or reduce to the extent possible the various undesirable material, weed seeds, other crop seeds, deteriorated or damaged seeds. Certified seed is the starting point to a successful crop as well as an important risk management tool. Certified seed uses systems to maximize genetic purity Off-types, other crop seeds, and weeds are guaranteed to be minimized. The blue tag is proof that you used certified seed to maintain the value traits of the crop. REFERENCES http://ecoursesonline.iasri.res.in/mod/page/view.php?id=17886 https://agritech.tnau.ac.in/seed_certification/seed_pul_greengram. html https://agritech.tnau.ac.in/seed/seedmultiplication.html https://agritech.tnau.ac.in/seed_certification/seed%20treatments% 20-%20Chemical.html https://agritech.tnau.ac.in/seed/Seed_seedsampling.html http://www.jnkvv.org/PDF/04042020081723Seed%20Germination %20Testing-3.pdf https://agritech.tnau.ac.in/seed/Seed_seedtesting.html https://agriculture.rajasthan.gov.in/content/agriculture/en/RSSOPC A-dep/seed-certification/process-of-certification/processing.html