Name
Date
Section
Group Member Names
Turn in to your TA along with your drawings today before you leave the lab.
1. (1 pt)
Power =
Magnification
of Objective
× Magnification
of Ocular
= Total
Magnification
Medium
High
2. (0.5 pts) Does the working distance increase or decrease as the magnification increases?
3. (0.5 pts) Does the light intensity increase or decrease as the magnification increases?
4. (0.5 pts) On your microscope, under which magnification can you view the largest
area on the slide?
5. (0.5 pts) On your microscope, under which magnification is the actual field of view
the smallest?
6. (3 pts) The figure of the circle on the right represents
the field of view as seen through a microscope at a total
magnification of 100×. At this total magnification, the W
distance across the middle of this visual field (from W to
Z) is about 1,600 μm. If you place a cell along this line,
you can estimate the cell’s size. For example, what is the
cell’s diameter if it ranges from W to Y?
Estimated cell diameter = ___________
X
Y
Z
Exercise 2 Answer Sheet: Microscopy and Cell Structure
Low
23
On these pages you will draw what you see and estimate the size of at least six specimens (including two wet mounts). Your TA will tell you which slides to draw.
Each drawing must include a scale bar.
Exercise 2 Answer Sheet: Microscopy and Cell Structure
DRAWINGS (3 PTS)
24
Human cheek cell (measure approx. cell diameter)
Onion cell (measure width of an individual cell)
Magnification ___________________________
Magnification ___________________________
Approximate size ________________________
Approximate size ________________________
Penicillium (measure size of conidia)
Dicot root (measure width of an individual cell)
Magnification ___________________________
Magnification ___________________________
Approximate size ________________________
Approximate size ________________________
Giant multipolar neuron (measure width of nucleus)
Bacillus (measure width of one bacterium)
Magnification ___________________________
Magnification ___________________________
Approximate size ________________________
Approximate size ________________________
Amoeba wet mount (measure cell size)
Magnification ___________________________
Magnification ___________________________
Approximate size ________________________
Approximate size ________________________
Euglena wet mount
Blood (measure width of an individual cell)
(measure approx. cell diameter)
Magnification ___________________________
Magnification ___________________________
Approximate size ________________________
Approximate size ________________________
Cuboidal epithelium (measure cell size)
Squamous epithelium (measure nucleus size)
Magnification ___________________________
Magnification ___________________________
Approximate size ________________________
Approximate size ________________________
Exercise 2 Answer Sheet: Microscopy and Cell Structure
Elodea wet mount (measure width of one cell)
25
Exercise 2 Answer Sheet: Microscopy and Cell Structure
Columnar epithelium (measure nucleus)
26
Skeletal muscle (measure cell size)
Magnification ___________________________
Magnification ___________________________
Approximate size ________________________
Approximate size ________________________
MINI-PRACTICAL: ID THE SLIDES BELOW
1.
2.
3.
4.
5.