1.
2.
3.
4.
Uses a light to form the image
Maximum resolution of about 0.2 micrometres (μm)
Not visible – ribosomes, ER, and lysosomes. Visible – Nucleus, Mitochondria
Maximum useful magnification = x 1500.
How much bigger the image
appears than the specimen sample is
Magnification
Optical
Microscope
Magnification =
size of image/size of object
Microscopes
Electron
Microscope
1. Uses electrons to form an image.
2. Maximum resolution of about 0.0002 micrometres (μm) - about 1000 times higher
than optical.
3. Visible – all organelles
4. Maximum useful magnification = x1,500,000.
Resolution
If a microscope
cannot
separate two
objects,
more
magnification
will not help to
see them
The better the
resolution, the
better the
microscope is able
to distinguish
between
two points that are
close together.
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