Certificate This is to certify that “chemistry project” on the topic To compare rates of fermentation of given sample of wheat ,gram floor, rice and potatoes has been successfully completed by Pranjali Agrawal of class XII E under the guidance of Mr. Gaurav Varshney in particular fulfillment of curriculum of central board of secondary education leading to award of annual examination of year 2023-24. Gaurav Varshney external examiner Acknowledgement I have taken effort in his project . However, it would have not been possible without the kind support and help of many individual . • I am highly indebted to my chemistry teacher for her valuable guidance which has sustained my effort in all stages of this project . • I would also like to thanks our In-charge mam – MRS. Neelam Maheshwari , my parents for their support and encouragement . • My thanks and appreciation also goes to my fellow classmates in developing the project and to the people who willingly helped me out with possible Index 1) 2) 3) 4) 5) 6) Aim Objective Introduction Theory Procedure Observation AIM To compare the rate of fermentation of given sample of wheat flour gram flour rice flour and potato using yeast . OBJECTIVE The purpose of the experiment is – to compare the rate of fermentation of the given samples of wheat flour , gram flour, rice flour and potatoes .I became interested in this idea when I saw some experiments on fermentation and wanted to find out some scientific facts about fermentation . The primary benefit of fermentation is the conversion of sugars and other carbohydrates , e.g., converting juice into wine, grains into beer, INTRODUCTION The purpose of the experiment is – to compare the rate of fermentation of the given samples of wheat flour , gram flour, rice flour and potatoes .I became interested in this idea when I saw some experiments on fermentation and wanted to find out some scientific facts about fermentation . The primary benefit of fermentation is the conversion of sugars and other carbohydrates , e.g., converting juice into wine, grains into beer, carbohydrates into THEORY Wheat flour , gram flour ,rice flour and potatoes contains starch as the major constituent . Starch present in these food materials is first brought into solution. In the presence of enzyme diastase , starch undergo fermentation to give maltose. Starch gives blue-violet color with iodine whereas product of fermentation starch do not give any characteristic color. When the fermentation is complete the reaction mixture stops giving blue-violet color with iodine solution. By comparing the time required for completion of fermentation of equal amounts of different substances containing starch the rates of fermentation can be compared . When the germination is complete the temperature is raised to MATERIALS REQUIRED PROCEDURE 1) Take 5 grams of wheat flour in 100 ml conical flask and add 30 ml of distilled water. 2) Boil the contents of the flask for about 5 minutes Filter the above contents after cooling , the filtrate obtained is wheat flour extract. 3) To the wheat flour extract. taken in a conical flask. Add 5 ml of 1% aq. NaCl solution. 4) Keep this flask in a water bath maintained at a temperature of 50-60 degree Celsius . 5) Add 2 ml of malt extract. 6) After 2 minutes take 2 drops of the reaction mixture and add to diluted iodine solution. 7) Repeat step 6 after every 2 minutes . When no bluish color is produced the OBSERVATION 1) Time required for the fermentation 2) Wheat flour — 10 hours 3) Gram flour — 12.5 hours 4) Rice flour — 15 hours 5) Potato — 13 hours 6) CONCLUSION – Rice flour takes maximum time for fermentation and wheat flour takes the minimum time for fermentation.