Bulacan State University Guinhawa, Malolos City, Bulacan The Impact of Love on Happy Hormones Among the Young Adults of Bulacan State University In Partial Fulfillment of the Requirements for the Subject Biological Psychology Submitted by: Balungcas, Pamela Rose L. Dabu, James Patrick R. Galang, Anne Jhasmine G. Llacer, Hannah Marie B. Oczon, Piel Joy B. Santos, Daniella Jenine R. Yap, Jose Andrew M. December 2022 INTRODUCTION Love is a phenomenon that all human beings will eventually experience as they live. It manifests in many forms as warm feelings towards family, child, spouse, pets, or friends (Tamam & Ahmad, 2019). Furthermore, love has become an important topic throughout history (Ferreira et al., 2018) and inspired various kinds of artistic expression in humankind (De Boer et al., 2012). Although it has long been studied, love remains an abstract concept associated with the feeling of deep affection towards someone. Is this a mere feeling, or is there a more profound chemical force behind it? In terms of love styles, the love towards another individual that causes strong affection and attraction is called romantic love (Acevedo et al., 2017). Eros or romantic love, is the love style that is the most common depiction of love in media (De Jong & Collins, 2017). Wherein, the love is based on passion, physical and inner attraction towards their special someone is the main driving force (Raffagnino & Pudu, 2018). In alignment with that, recent studies involving love had aimed to clarify the present connection between chemicals and love (Magon & Kalra, 2011). The hormone oxytocin and the neurotransmitters serotonin, dopamine and endorphins are generally the four chemicals associated with love (Franza & Cevone, 2014). These chemicals are often grouped and generally considered by most as “happy hormones” (Ferreira et al., 2018). Hormones, produced in the endocrine system (Toler, 2019; Mandal, 2019) are secreted into the bloodstream, which then regulates our body's processes by sending messages to different body parts (Mandal, 2019; Raypole, 2019; Medline Plus, 2019). Although these chemical messengers flow throughout our system, they only affect specific cells with a corresponding receptor site. With that, it is kind of a key-and-lock mechanism. An effect will arise if the hormone fits the receptor site. However, there won't be any response if the hormones and receptor sites do not match up (National Institutes of Health, 2019). Once the hormones reach their target cell, they influence it by altering cellular activity; a small amount of hormone may result in a massive change in the body (Medline Plus, 2019). Neurotransmitters, on the other hand, are chemical substances released to a synaptic gap, capable of transmitting signals from a neuron to a target cell. Unlike hormones that are produced in the endocrine system, neurotransmitters are created in the nervous system and usually take milliseconds to form a response. Hence the site of their release and site of action is the thin line that distinguishes hormones and neurotransmitters (Panawala, 2017). These chemicals influence an individual's mood and social behavior (Magon & Kalra, 2011). Including, and not limited to, emotions, affection, and specifically, love (Elsayed et al., 2022). Since several studies have already established concrete evidence that happy hormones affect love, this paper aims to determine the extent to which the feeling of being in love affects an individual's level of happy hormones. In addition, the researchers would like to investigate it with young adults, for studies have shown that during the age of 20-22, individuals have more opportunities to engage in more serious relationships of their own choosing (Tillman et al., 2019). THEORETICAL AND LITERATURE SUPPORT The purpose of this research is to determine the relationship between stimulus, love, and happy hormones. As a result, the Stimulus-Organism-Response (SOR) model theory is employed. This theory was proposed by Albert Mehrabian and James A. Russel in 1974. According to the theory, environmental stimuli influence an individual's internal state (Kishor, 2020). A specific stimulus will work in order to elicit the desired response from the organism. The organism, as the center and channel of this phenomenon, will receive the stimulus and respond to it. In this study, the IV (love or feelings of love) is the stimulus. The stimulus will be received by the respondents, who are the organism. The expected response are the changes in levels of happy hormones that the researchers are anticipating. Love and Happy Hormones Despite being neglected in the past by experimental sciences, emotions and feelings have become the focus in neuroscientific research, particularly the most typical human feeling, love. On the other hand, dopamine, oxytocin, endorphin, and serotonin are happy chemicals that burst out when triggered by what we perceive as something that makes us happy (Breuning, 2012). Several concepts have been associated with happy hormones and the most recent and popular concept that researchers try to connect with happy hormones is love. A number of studies have found a link between love and happy hormones like dopamine, serotonin, oxytocin, and endorphin. In a study about neurochemistry of love, Tamam & Ahmad (2019) have found out that people who claim that they are in love release neurotransmitters, especially the happy hormones and other chemicals related to euphoric sensations. Romantic couples have higher levels of dopamine and oxytocin that are closely related to pleasure (de Boer et al., 2012; Tamam & Ahmad, 2019; Field et al., 2016 & Takahashi et al., 2015) and attachment (Tamam & Ahmad, 2019 & Perrotta, 2020) which are the common aspects of love. The activation of dopamine will also lead for serotonin levels to drop and activate the brain's pleasure centre which is responsible for the madness and obsessive aspect of love (Tamam & Ahmad, 2019; Aleksidze, 2018 & Langeslag et al., 2012). The constant feeling of euphoria that people experience when in love is attributed to endorphins. People with high levels of endorphins feel less pain and fewer negative effects of stress (Tamam & Ahmad, 2019; Khajehei et al., 2018; Chadha, 2019 & Barymova, 2022). Love and Dopamine Another happy hormone is dopamine. As previously stated, oxytocin influences the release of other feel-good hormones, one of which is dopamine. Oxytocin and vasopressin stimulate dopamine release by interacting with the dopamine reward system. People perceive this brain activity as a reward, making love have the same euphoric feeling as a sense of accomplishment (de Boer et al., 2012 & Takahashi et al., 2015). Similarly, de Boer et al. (2012) stated that the dopaminergic pathways involved in love and pair-bond formation were largely similar to those involved in addiction, resulting in a vague similarity of feelings in love and addiction. In an experiment conducted by Field et al. (2016), people with romantic partners were shown facial photos of their romantic partners, highly familiar acquaintances, close long-term friends, and a less familiar person. Then using functional magnetic resonance imaging (fMRI), their dopamine levels were observed. Results showed that dopamine's reward system activated when each photo was shown but with different levels—the photo of their partner being the highest. This suggested that dopaminergic reward pathways contribute to the arousal of the components of romantic love. Love and Oxytocin Oxytocin, also known as the love hormone, is linked to reproduction, particularly sexual behavior. The presence of oxytocin during sex increases pleasure. Because oxytocin is released through physical touch, people perceive this as feelings of closeness (Perrotta, 2020). This phenomenon results in people mistaking the release of oxytocin as the feeling of being in love. Despite the possibility of being an illusioned feeling, oxytocin is still believed to be associated with love as Carter (2022), argued that oxytocin helps regulate emotional states including love. It acts on target tissues and neuroendocrine pathways, affecting the release of other feel-good hormones. Furthermore, love, in Science, is described as selective behavior and attachments and oxytocin is a component of the biological system that secures attachment by releasing feelings of pleasure through physical contact. Love and Serotonin Unlike other hormones that increase when one is in love, serotonin levels decrease (de Boer et al., 2012; Aleksidze, 2018 & Langeslag et al., 2012). This is because serotonin has an inverse relationship with corticosteroids. Furthermore, low serotonin levels have been linked to some psychiatric disorders such as obsessive compulsive disorder (OCD). According to Langeslag et al. (2012), men and women's serotonin levels differ when they are in love. Women have higher levels when they are in love, whereas men have the opposite effect. Additionally, serotonin is in charge of activating brain structures during sexual activities. Serotonin is responsible for the fact that sex is enjoyable rather than painful (Aleksidze, 2018). It is because serotonin improves the transmission of pleasant feelings during sex, overlapping the pain that sex causes and creating a feeling of bliss associated with love. Love and Endorphins Literature suggests that endorphins may be involved in the regulation of sexual function and love (Khajehei et al., 2018; Chadha, 2019 & Barymova, 2022). Like opiates, endorphins can also calm anxiety, relieve pain, and reduce stress. They produce a general sense of well-being, including feeling soothed, peaceful, and secure which people attribute to the belief that being with the person we love calms us (Chadha, 2019). With the security and peace that endorphins give, people with high levels of endorphins are most likely to experience less stress, enjoying a happy relationship with their partner (Khajehei et al., 2018). RESEARCH GAP While all studies show a positive correlation between love and happy hormones, research gaps among those studies still exist. Most available studies gathered their data through experimental and observational studies using medical technologies such as fMRI. However, most studies such as Khajehei et al. 2018, Takahashi et al. 2015, Hasin et al. 2017 & de Boer et al. 2012 used animals in their experimental research. These findings have not been supported by research into human behavior. This entails a lack of association in humans since animals are believed to be driven by pleasure rather than emotions that animals do not have. Nonetheless, these findings have revealed a gap in the reliability and validity of human concepts. Since the study found a gap in the previous research, the paper aims to explore the connection between the variables in human perspective. While previous studies use animals as the focus of their experiment, the paper aims to fill the gap by using 3rd year psychology students of Bulacan State University as participants to know if love can predict the level of their happy hormones. CONCEPTUAL FRAMEWORK The conceptual framework of the study shows the procedural measurement of the two variables in an experimental design. The Independent variable (Love) will measure whether it will have an impact on certain happy hormones as the arrow demonstrated. While the study manipulated the independent variable, the level of happy hormones will be quantified and properly documented. As the experiment suggests, the extraneous variable is inevitable however controllable. With this, the researchers have certainty of managing the factors that might affect the study. Furthermore, the study will take place in a regulated experimental laboratory to reach the validity and reliability of the study. STATEMENT OF THE PROBLEM The study aims to determine if love can predict the level of happy hormones in the participants. Love will be in general form, other subtypes and/or kinds will not be included in the study. However, the four kinds of happy hormones will be covered, and measured individually, and in groups. ● Can love positively predict the level of dopamine in the 2nd year psychology students of Bulacan State University? ● Can love positively predict the level of oxytocin in the 2nd year psychology students of Bulacan State University? ● Can love positively predict the level of serotonin in the 2nd year psychology students of Bulacan State University? ● Can love positively predict the level of endorphin in the 2nd year psychology students of Bulacan State University? ● Can love positively predict the level of happy hormones in general in the 2nd year psychology students of Bulacan State University? HYPOTHESIS Applying the statement of the problem, the following hypothesis was made specifically for this study. The researchers used a two-tailed hypothesis to predict the attitude of the Independent and Dependent Variables: ● Love positively predicts the level of dopamine in the 2nd year psychology students of Bulacan State University. ● Love positively predicts the level of oxytocin in the 2nd year psychology students of Bulacan State University. ● Love positively predicts the level of serotonin in the 2nd year psychology students of Bulacan State University. ● Love positively predicts the level of endorphin in the 2nd year psychology students of Bulacan State University. ● Love positively predicts the level of happy hormones in general in the 2nd year psychology students of Bulacan State University. SCOPE AND DELIMITATION This study is to focus on determining the effect of love on happy hormones. To be more specific, the study included the four kinds of happy hormones: Dopamine, Oxytocin, Serotonin, and Endorphins. Moreover, the study encompasses Positive Psychology and covers one of its components, Love. Furthermore, given that the study is quantitative in nature, it will focus on using the experimental method to have a valid and reliable result and justification of the causality. Measurement of Romantic Love will be used to measure the extent of the variables. The study is delimited on being quasi-experimental as a design of quantitative research. The use of simple random sampling is limited to the 3rd year students of Bulacan State University–Main Campus. Further, the data-gathering process is delimited by utilizing a questionnaire with 9 point-Likert scales. Through this, the researchers are certain to get the validity and reliability of the result. According to the study of Primasari & Yuniarti (2012), the subject matter of this study is restricted to the age group of adolescents and young adults, as teenagers’ happiness can be rooted to love being the factor that they adore and are adored by. The study is also demarcated to love as a facet of Positive Psychology yet barely touches other facets to widely explain the focus of the study. The objective of the study is to determine the impact of love on happy hormones. Relationships and causality will be both included and limited to these two. Moreover, the researchers are delineated to only evaluate the variables and control the possible extraneous variables. Yet, the study will provide real-world causal explanations and can help predict the unexplained impact of the selected variables. The instrument tools of measurement are limited to measuring the state of “being in love” of the participants. The procedural method to assess the level of happy hormones will be conducted in the laboratory. Limitations will guide the whole experiment. To get the true value of the result, choosing the appropriate duration of the study has a factor. Therefore, the study will run on February 14, 2023, and only to the said date. METHOD Research Design This study will utilize a quantitative-quasi-experimental design, specifically, nonequivalent group design to establish a cause-and-effect relationship between an independent and dependent variable. Typically, quasi-experiments examine the long-term effects of therapies outside the laboratory (Cook, 2015). Furthermore, a nonequivalent group design is applied to assess the relative effects of treatments assigned to groups of participants nonrandomly (Reichardt, 2015). Therefore, the gathered information on the relationship between the feeling of being-in-love and the level of happy hormones of 3rd-year college students of Bulacan State University will make the research study intelligible by utilizing the nonequivalent group design. Participants The non-probability sampling technique, specifically purposive sampling and non-random assignment, will be used in this study. A total of fifty (N=50) 3rd year college students from Bulacan State University of Malolos, whose ages range from 20-22 years old, will participate in this study. Participants’ demographic is based on the study of Hogan (2018) which posits that romantic love is mostly evident in young adults for the reason that they have more opportunity to engage in romantic relationships of their own choosing. Besides, regardless of their gender, they will be selected according to the non-random criteria and will be divided into two groups using the romantic love questionnaire; the in-love group (n=25) and not in-love group (n=25). This sampling group will be given an informed consent form before the experiment. Instruments The researchers will administer a questionnaire from Rubin (1970) entitled Measurement of Romantic Love to acquire data necessary to yield results from the investigations. The MRL scale measures respondents' attitudes toward a particular person, mainly their initial conceptualization of romantic love. It consists of 13 statements, such as "I would do almost anything for ______", and "I would greatly enjoy being confided in by ______", answerable through a 9-point Likert scale, where scaling is given as follows: Not at all true/ disagree completely (1); definitely true/ agree completely (9). Validity reveals whether an instrument measures what it is supposed to measure. It implies that whatever scores are obtained from the instrument should enable the researcher to draw conclusions from the sample of the population under investigation (Creswell, 2008). The questionnaire designed for this study was subjected to a validation process for face validity. Face validity has been defined by McBurney (1944) as the idea that a test should appear superficially to test what it is supposed to test. Polit and Hungler (1993) define reliability as the degree of consistency with which an instrument measures the attribute it is designed to measure. The questionnaire, as used in the previous studies, was found to be reliable as it revealed consistency in responses and is internally consistent with Cronbach's coefficient alpha value. Procedures The experiment will take place at Bulacan State University College of Social Science Philosophy laboratory. Prior to the actual experiment, the researchers will be disseminating the Measurement of Romantic Love questionnaire to the target population. The items of the MRL assess the affective components of love and the attitudes a person holds about others. With that, the participants will then be divided into two groups based on their scores from the MRL: 1-77 = not in love, n=25; 78-117 = in love. This split is necessary to identify the independent variable by having two categories: in love and not in love. For an accurate dependent variable measurement, venous blood will be collected from the subjects which will then be centrifuged and evaluated by biologists to see whether there is an increase in the participant's level of happy hormones; serotonin, oxytocin, dopamine, and endorphin. On the actual day of the experiment, the procedure will be explained to the participant upon their arrival at the laboratory. They will be informed that they could terminate participation at any time and will sign an informed consent. The participant will be accompanied to a chamber, where the phlebotomist will draw their blood. Following the blood extraction, the participant was debriefed and dismissed. Data Analysis Plan The data to be collected will undergo analysis using descriptive and inferential statistics. Descriptive statistics will be used to determine the mean, standard deviation, and skewness of the variable. For inferential statistics, an independent sample t-test will be used to compare the means of two independent groups; those who are in love and those who are not. Afterward, Cohen's d will be used to measure the effect size of the independent variable on the dependent variable as well as testing of normality and homogeneity to see if the data did not violate the assumptions necessary for an independent samples t-test. References: Acevedo, B. P., Aron, A., Fisher, H. E., & Brown, L. L. (2011). Neural correlates of long-term intense romantic love. Social Cognitive and Affective Neuroscience, 7(2), 145–159. doi:10.1093/scan/nsq092 Aleksidze, N.G. (2018). The quantitative distribution of the hormones of love and neurotransmitters at psycho emotional stresses. Journal of Neurology, Psychiatry and Brain Research, 3(5), 1-8. Barymova, A.S.(2020). Chemistry of love. A. S Pushkin Department of Foreign Languages. Breuning, L. G. (2012). Meet your happy chemicals. System Integrity Press. Carter, C. S. (2022). Oxytocin and love: Myths, metaphors and mysteries. Comprehensive Psychoneuroendocrinology, 9(100107), 100107. https://doi.org/10.1016/j.cpnec.2021.100107 Chadha, N. K. (2019). A psychological study on the positive impacts of experiencing love. ProQuest, 7(3), 513–518. https://www.proquest.com/docview/2618176229 De Boer, A., van Buel, E. M., & Ter Horst, G. J. (2012). Love is more than just a kiss: A neurobiological perspective on love and affection. Neuroscience, 201, 114–124. doi:10.1016/j.neuroscience.2012 De Jong, M., & Collins, A. (2017). Love and looks: A discourse of romantic love and consumer culture. Acta Academica, 49(1). doi:10.18820/24150479/aa49i1.5. Elsayed, A. S., Azab, A. E., & Etwebi, K. E. (2022). An overview of oxytocin: Chemical structure, receptors, physiological functions, measurement techniques of oxytocin, and metabolism. Journal of Clinical Research, 11(4):1-11. https://doi.org/10.31579/2690-1919/256 Ferreira, F. S., da Costa, R. L., Pereira, L., Jerónimo, C., & Dias, A. (2018). The relationship between chemical of happiness, chemical of stress, leadership, motivation and organizational trust: A case study on Brazilian workers. Journal of International Business and Economics, 6(2), 89–100. https://doi.org/10.15640/jibe.v6n2a8 Field, T., & Touch Research Institute, University of Miami, Miller School of Medicine, USA. (2016). Romantic love. International Journal of Behavioral Research & Psychology, 185–190. https://doi.org/10.19070/2332-3000-1600033 Francesco, F., & Cervone, A. (2014). Neurobiology of love. Psychiatria Danubina, 26(Suppl 1), 266-268. Hasin, D., Pampori, Z. A., Aarif, O., Bulbul, K.H., Sheikh, A. A. & Bhat, I. A. (2018). Happy hormones and their significance in animals and man. International Journal of Veterinary Sciences and Animal Husbandry, 3(5) 100-103. Khajehei, M. & Behroozpour, E. (2018). Endorphins, oxytocin, sexuality and romantic relationships: An understudied area. World J Obstet Gynecol, 7(2): 17-23. Langeslag, S. J. E., van der Veen, F. M., & Fekkes, D. (2012). Blood levels of serotonin are differentially affected by romantic love in men and women. Journal of Psychophysiology, 26(2), 92–98. https://doi.org/10.1027/0269-8803/a000071 Lee, J. A. (1973). The colors of love: An explanation on the way of loving. Ontario: New Press. Magon, N., & Kalra, S. (2011). The orgasmic history of oxytocin: Love, lust, and labor. Indian Journal of Endocrinology and Metabolism, 15(7), 156. doi:10.4103/2230-8210.84851 Marazziti, D., & Canale, D. (2004). Hormonal changes when falling in love. Psychoneuroendocrinology, 29(7), 931–936. doi:10.1016/j.psyneuen.2003.08.006 10.1016/j.psyneuen.2003.08.006 Morrow, G. D., Clark, E. M., & Brock, K. F. (1995). Individual and partner love styles: Implications for the quality of romantic involvements. Journal of Social and Personal Relationships, 12(3), 363–387. doi:10.1177/0265407595123003 Perrotta G. (2020). Oxytocin and the role of “regulator of emotions”: Definition, neurobiochemical and clinical contexts, practical applications and contraindications. Archives of Depression and Anxiety, 6(1): 001-005. https://dx.doi.org/10.17352/2455-5460.000043 Primasari, A., & Yuniarti, K.W. (2012). What makes teenagers happy? An exploratory study using indigenous psychology approach. International Journal of Research Studies in Psychology, 1(2), 53-61. doi: 10.5861/ijrsp.2012.v1i2.80 Raffagnino, R., & Puddu, L. (2018). Love styles in couple relationships: A literature review. Open Journal of Social Sciences, 6(12), 307–330. https://doi.org/10.4236/jss.2018.612027. Rubin, Z. (1970). Measurement of romantic love. Journal of Personality and Social Psychology, 16(2), 265–273. doi:10.1037/h0029841 Takahashi, K., Mizuno, K., Sasaki, A.T., Wada, T., Tanaka, M., Ishii, A., Tajima, K., Tsuyuguchi, N., Watanabe, K. Zeki, S., & Watanabe, Y. (2015) Imaging the passionate stage of romantic love by dopamine dynamics. Front. Hum. Neurosci, 9, p. 191. https://www.frontiersin.org/articles/10.3389 Tamam, S., & Ahmad, A. H. (2019). The healing power: A review on the various aspects of the love-pain relationship. Malaysian Journal of Science Health & Technology, 3. https://doi.org/10.33102/mjosht.v3i1.58 Tillman, K. H., Brewster, K. L., & Holway, G. V. (2019). Sexual and romantic relationships in young adulthood. Annual Review https://doi.org/10.1146/annurev-soc-07301802265 of Sociology, 45, 133-153.