Ananth Kashibhatla 30 January 2023 Environmental Microbiology Extra Credit Seminar – January 23 – Dr. Fahrenfeld In Dr. Fahrenfeld;s seminar titled “Microplastics in Our Waters: Sources, Fate, and Biofilms”, she takes an in-depth look at how humans are adversely affecting the contents we put out in our waters affect our ecosystem and environment. She starts her seminar with talking about having a lack of circular economy for plastics is hurting us more than we think. Recycling is heavily further emphasized and many marine life organisms such as fish end up mistaking plastic debris in the ocean for food. Next, Dr. Fahrenfeld delves into the specifics of these plastics, particularly called microplastics, which are manmade polymers that are less than 5 mm of diameter. The two types are primary microplastics, which are manufactured in small sizes and secondary microplastics, which are derived from larger plastic, either photophysical or mechanical degradation. Most of the deadly plastics that are deadly for organisms are these types of plastics or microplastics. Then, in the conceptual model of fate and transport, several sources contribute to this microplastic accumulation, for example but not limited to atmospheric deposition, land-based riverine sources, at-sea pollution, and UV & oxidative weathering. Over time, you end up accumulating these wastes, which end up becoming harmful to the organisms that reside in these waters. Furthermore, the lack of evidence of these ecological impacts is the reason why many people are not taking this issue seriously. However, California is the first state that is taking these issues seriously and is the first state to regulate microplastics, especially in the context of drinking water. In the Raritan-Hudson estuary case study, it was found that a lot of these microplastics are prevalent in the river plume that originally came from the coastal ocean, with the sediments accumulating and becoming a biohazard for peoples’ drinking waters. We can study these effects by measuring the concentrations found in sources such as wastewater influent + effluent, stormwater and zooplankton in low and high flows via nets. Dr. Fahrenfeld goes on to then talk about different concentrations of biowastes in the context of the aforementioned low and high lows with small and large MP. In addition, different polymer types were also found in these wastes such as other plastic, vinyl copolymer, polyester, polystyrene, polypropylene, rubber and polyethylene, with the majority of the uses extending to packaging, building/construction, automotive, electrical/electronic, and others. Then, Dr. Fahrenfeld also points out a graphic that shows the different types of polymers in the context of its source, such as wastewater effluent, wastewater influent, wastewater solids and compost. Another method that was used to study the prevalence of microplastics is fate, which is the uptake into zooplankton sources. Different methods are devised to study microplastic concentrations in these microorganisms. Nitric digestion is also another point that was mentioned where you could use these methods to “digest” these wastes and PE2, PMMA-1 and PC concentrations were taken into account for this study. The sources that you can find these wastes are usually in the wastewater and stormwater sources. As such, it was shown that wastewater treatment plants are actually effective in removing these microplastics. Different biofilms also exist, such as glass microbeads, large polyethylene, small polyethylene, large polystyrene, and small polystyrene; we compared these concentrations in WW Influent and Raritan River sources. An effective method to disinfect these wastes is the use of peracetic acid but it was found that it’s only effective when the LDPE is approximately equivalent to Wood.