Ammonia Assay Kit_Calibration curve
Preparation of two NH3 sample
y = 282.19x-10.01
1. 100 ppmv, 0.3 L in 3 mL water, theoretical [NH3]: 0.41 mM
2. 100 ppmv, 0.3 L in 1 mL water, theoretical [NH3]: 1.22 mM
Intensity
Experimental [NH3]
Theoretical [NH3]
Sample 1
21.33
0.11 mM
0.41 mM
Sample 2
85.07
0.34 mM
1.22 mM
Ammonia Assay Kit (MAK310) was used to build up the
calibration curve following the standard protocol (technical
bulletin from Sigma).
Standard solution: 0.1, 0.25, 0.5, and 1 mM NH4Cl
Principle
Fluorescence (λem=450 nm )