Introduction to the
Microscope
Care
Parts
Focusing
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Always carry with 2 hands
Only use lens paper for cleaning
Do not force knobs
Always store covered
Keep objects clear of desk and cords
Parts of a Compound
Microscope
compound
microscope
Eyepiece
Body Tube
Revolving Nosepiece
Objective Lens
Stage
Clips
Diaphragm
Light
Arm
Stage
Coarse Focus
Fine Focus
Base
Compound Microscope
• A microscope is a
very powerful
magnifying glass
• A microscope
helps you see
things like cells up
close
Eyepiece
• View the specimen
through the
eyepiece
Stage Clips & Objectives
• Stage clips hold the
slide in place
• Low power objective
is used to focus the
microscope (short &
fat)
• High power objective
is used to view
details of a specimen
Coarse Adjustment, Fine
Adjustment, & Base
• Coarse adjustment
focuses adjustment
• Fine adjustment fine
tunes & gives detailed
focus(usually smaller
than coarse
adjustment knob)
• Base is where the
microscope rests
Stage
• Stage is part
where the slide
rests
• Mirror (or light
source) directs
light upwards onto
the slide.
Diaphragm
• Diaphragm allows
light in
Nosepiece
• Nosepiece is the
rotating device
that holds the
objectives (lenses)
Arm
• Arm is the part
where you carry
the microscope
Can you name the parts of a
compound microscope?
Answers
1) base
2) mirror (light source)
3) diaphragm
4) stage
5) stage clips
6) low power objective lens
7) high power objective lens
8) nosepiece
9) arm
10) fine focus knob
11) body tube
12) coarse focus knob
13) eyepiece
Types of Microscopes
Light microscope
• (for small objects similar to the way
binoculars magnify
objects far away.)
The Compound Light
Microscope
http://www.cas.muohio.edu/~mbi-ws/microscopes/compoundscope.html
• May have many lenses
that magnify the object
• Eyepiece = 10x
magnification
• Objective = 10x (low) or
40X (high)
•Total magnification=
•eyepiece X objective
•ex: 10x X 40x =
400x total
magnification
Compound Light Microscope
• Powered by Light & Lenses
a) Powers of Magnification
• Ocular Lens= _________
• Low Power Objective= _______
• Medium Power Objective=
_______
• High Power Objective= _______
• Calculating Total
Magnification= ____________
b) Resolution
c) Parts
Stereomicroscope
• To look at large things that light
cannot pass through (one eyepiece
for each eye)
Stereomicroscopes
http://scientificsonline.com/product.asp_Q_pn_E_3119600
http://www.martinmicroscope.com/MicroscopePages/Stereomicroscopes.htm
Stereomicroscopes
•3 - D image
•can only magnify 10x
to 600x
Electron Microscope
• can magnify more
than 500,000x
House Fly
•
http://www.pbrc.hawaii.edu/bemf/microangela/hfly.htm
• Place the Slide on the
Microscope
• Use Stage Clips
• Click Nosepiece to the lowest
(shortest) setting
• Look into the Eyepiece
• Use the Coarse Focus
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Follow steps to focus using low power
Click the nosepiece to the longest objective
Do NOT use the Coarse Focusing Knob
Use the Fine Focus Knob to bring the slide
into focus
What can you find on your slide?