002 Aquaporin Modeling
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Working in Google Docs
1. In the Google Doc, one member of the group should click “File”, then “Make a copy” to create an editable version of the Google Doc to your Google
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2. The SAME team member should click on “Share” (blue button in upper right) and enter the email addresses of the rest of the team members.
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3. All team members should open up the shared document to work on the same Google Doc to complete all parts of the activity.
Frog oocytes are naturally impermeable to water, and therefore were well
suited for early studies on aquaporins. In order to induce expression of
aquaporins in the oocytes, aquaporin mRNA was injected into oocytes to
drive protein expression and membrane incorporation of the channel. In
the following examples, model the flow of water through aquaporins under
different solvent conditions. Recall that hypotonic means “low”, hypertonic
means “high”, and isotonic means “same”.
Frog Oocytes in a Hypotonic Salt Solution
Frog Oocytes + Aquaporin mRNA in a Hypertonic Salt
Solution
Lower
[Solute]
Higher
[Solute]
Lower
[Solute]
Frog Oocytes + Aquaporin mRNA in an Isotonic Salt
Solution
Higher
[Solute]
Frog Oocytes + Aquaporin mRNA in a Hypotonic Salt
Solution (Note membrane potential!)
Equal
[Solute]
+
+
+
–
–
Equal
[Solute]
+
–
+
+
+
–
Higher
[Solute]
–
–
+
–
+
+
+
Lower
[Solute]