2nd Edition 2nd Edition The Discover Biology for GCE 'O' Level Science Workbook (2nd Edition) is specially developed to complement the Discover Biology for GCE ‘O’ Level Science (2nd Edition) textbook. Designed to support learning with understanding, this workbook is written in line with the 2013 GCE Ordinary Level Science (Biology) syllabus from the Ministry of Education, Singapore. Its wide-ranging exercises build foundational knowledge and develop critical higher-order thinking skills. Workbook (Teacher’s Edition) Reinforces concept development • Concept mapping helps students review and integrate key concepts taught in a chapter. 2nd Edition Shapes independent learners and critical thinkers • Self-evaluation tool at the end of every worksheet helps students monitor their learning progress. • A variety of context-based and data-based questions challenge students to train up their higher-order thinking skills. Builds skills and exam confidence • Thought Process in worked examples guides students through essential points to consider when answering free-response questions. • A Model Examination Paper gives further practice and boosts exam confidence by helping students familiarise with the exam format. Lam Peng Kwan • Eric Y K Lam Christine Y P Lee Other components • Practical Book • Textbook Lam Peng Kwan Eric Y K Lam Christine Y P Lee ISBN 978-981-01-1730-6 Workbook Teacher's Edition (S)SciB_WB&TE Cover.indd 1 9/24/12 10:17 AM 2nd Edition Workbook Teacher's Edition Lam Peng Kwan • Eric Y K Lam • Christine Y P Lee Tittlepage TB-WB-PB-Sci.indd 3 8/29/12 10:23 AM © 2007, 2013 Marshall Cavendish International (Singapore) Private Limited © 2014 Marshall Cavendish Education Pte Ltd Published by Marshall Cavendish Education Times Centre, 1 New Industrial Road, Singapore 536196 Customer Service Hotline: (65) 6213 9444 E-mail: tmesales@mceducation.com Website: www.mceducation.com First published 2007 Second edition 2013 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without the prior permission of the copyright owner. Any requests for permission should be addressed to the Publisher. Marshall Cavendish is a registered trademark of Times Publishing Limited. ISBN 978-981-01-1730-6 Printed in Singapore (S)SciBioWBTE_Imprint/Acknow.indd 2 12/12/16 9:55 am ACKNOWLEDGEMENTS The publisher would like to acknowledge the following who have kindly given permission for the use of copyright material: Cover Page Nudibranch © Kelpfish/Dreamstime.com; 3 ER & mitochondria © Don W. Fawcett/Science Photo Library; 4 Plant cells © Kristian Peters - Fabelfroh(own photo)/Wikimedia Commons/CC-BY-SA-3.0(http://commons.wikimedia.org/wiki/ File:Plagiomnium_affine_laminazellen.jpeg); 7 Red blood cells © Bobjgalindo(own photo)/ Wikimedia Commons/CC-BY-SA-3.0(http://commons.wikimedia.org/wiki/File:Reactive_ lymph.JPG); 47 Stomata © Yunxiang987/Dreamstime.com; 60 Blood cells © Mkkans/ Dreamstime.com; 103 Sarracenia flower © Noah Elhardt(own work)/Wikimedia Commons/ Public domain(http://commons.wikimedia.org/wiki/File:Englishtitles2-1.jpg) While every effort has been made to contact copyright holders of material reproduced, we have been unsuccessful in some instances. To these copyright holders, we offer our sincere apologies and hope they will take our liberty in good faith. We would welcome any information, which would enable us to contact the copyright holders/owners involved. iii (S)SciBioWB_Imprint/Acknow.indd iii 8/25/12 5:18 PM Preface This Workbook is written specially for students taking the GCE ‘O’ Level Science (Biology) examination. To enhance learning and reinforcement, the topics in this Workbook are arranged in the same sequence as the topics in the Textbook. Connect It! WORK ON IT! Circle the question( s) you have answered incorrectly. Then, textbook to strengthe revisit the relevant n your understanding section(s) in the of the key concept( s). Students fill in a concept map using keywords from a chapter, in order to strengthen key concepts taught in that chapter. Date: Class: CONNECT IT! Discuss with your using keywords learnt in the chapter. Complete the concept map below can be extended. classmates how the concept map Organ system Red blood cell specialised cells Cell Tissue Organ Xylem Root Organism This section helps students monitor their progress in learning. Questions are categorised based on the learning outcomes in the Textbook. Students are asked to circle the questions they have answered incorrectly, and refer to the relevant section in the Textbook for further revision. Cell membrane Cytoplasm Nucleus which which which surrounds Contains chromatin (DNA) Mitochondria Ribosomes Energy source for cells Synthesise proteins © 2013 Marshall Cavendish International Is partially permeable and encloses the cytoplasm contains organelles such as (Singapore) Private Limited Vacuoles Store substances within the cell Chloroplasts (in plant cells) Site of photosynthesis Worksheet 1 are classified (d) Describe the characteri stics of enzymes FreeResponse Textbook section(s) 5.1 5.1 2 3 4, 5, 7 1, 3, 5 5.1 2 5.2 5 3, 6, 7 1, 2, 4 2 5.2 1 5.2 Work On It! vessel hair cell Cell wall plants ) (only in made up of Structured 1 1 Link to the Textbook chapter for easy reference. for Chapter 2 Cells MultipleChoice enzymes (e) Describe the mode of enzyme action based on the ‘lock-and-k ey’ hypothesis (f) Explain how conditions such as temperature and pH can affect the rate of an enzyme-catalysed reaction Worksheet 1 ) ( Name: Questions Learning objectives (a) Define an enzyme (b) State the function of (c) Explain how enzymes 1 © 2013 Marshall Cavendish International (Singapore) Private Limited Worksheet 4 (S)SciBioWB_04.indd 37 37 8/25/12 4:46 PM Learn & Apply A worked example and related practice questions show students how to answer essay-type and structured questions. TEST IT! Test It! A variety of questions with different difficulty levels is provided. -Choice Questions Section A: Multiple in the brackets provided. Choose the correct answer, Enzymes are 1. A B C D 2. 3. and write its letter . up in chemical reactions proteins that are used any temperature chemical reactions at proteins that catalyse living organisms chemical reactions in substances ( C ) proteins that catalyse compounds into simpler the breakdown of complex proteins that speed up would be the most to remove stains. Which biological washing powders Enzymes are added to chocolate milk stains? effective enzymes in removing Amylase and catalase A B Amylase and maltase ( D ) C Protease and catalase D Protease and lipase temperatures. out in water at different experiment that was carried The diagram shows an (d) Name two conditions or factors that had to Any two of the following: be kept constant in this Temperature / pH / Amount experiment. of enzyme used water LEARN & APPLY mixture of amylase and starch in a Visking tubing nse Questions. try to answer the Free-Respo example below, then Go through the worked sugar at 30-second the presence of reducing The water was tested for detected most rapidly? would reducing sugar be A B C D intervals. At which temperatur 10°C 20°C 40°C 100°C e is note that the question Thought Process It is important to take to answer the questions. Giving examples will help asking for plants. ( C ) Answer Points to cover in plants. speed up chemical reactions complete. 1. Enzymes help to will take a long time to these reactions. many reactions in plants enzymes to speed up 2. Without enzymes, esis and respiration require stored starch into glucose 3. For examples, photosynth in seed germination. Enzymes will digest the important 4. Enzymes are also and grow. for embryo to germinate © 2013 Marshall Cavendish 28 Worked Example of enzymes in plants. Explain the importance International (Singapore) Section B: Struc tured Questions Private Limited Answer the followi Worksheet 4 ng questions 1. in the spaces provided. X is an unknow n substance extract the ability of X ed from a living to bring about organism. A studen the hydrolysis boiling water t was required of starch. She (100°C) and the to investigate set up two water other contain ing water at 36°C. baths, one contain ing The student used the water baths • Test tube 1: 1 cm3 of X (pre-tre and set up the reaction mixture • Test tube 2: ated s as follows: at 100°C for 1 cm3 of X (pre-tre 1 minute) + 9 • Test tube 3: ated at 36°C cm3 of distille for 1 minute) 1 cm3 of X (pre-tre d water + 9 cm3 of distille • Test tube 4: ated at 100°C d water 1 cm3 of X (pre-tre for 1 minute) 3 + 9 • Test tube 5: ated cm of at starch 36°C for 1 minute 1 cm3 of distille ) + 9 cm3 of starch d water + 9 cm3 of starch The reaction mixture s were placed the student tested the mixture in the water bath that contain ed water Benedict’s test. in Her observations each test tube for the presen at 36°C. After 15 minute s, were as follows ce of reducin : g sugar using Test tube Observation for Benedict's test 1 Mixture remain ed blue 2 Data-based questions are provided to prepare students for the current examination format. The questions involve various kinds of data, to provide practice in interpreting and manipulating different kinds of data. 8/25/12 5:24 PM Mixture remain ed blue 3 International (Singapore) 35 Private Limited ed blue Mixture turned from blue to orange -red Mixture remain ed blue (a) Based on the X breaks down student's observ ations, what is starch (a non-red ucing sugar) into (b) What eviden ce X is specific in (test tube 3), Worksheet 4 © 2013 Marshall Cavendish Mixture remain 4 5 suppor ts that action. That is, substance X it hydrolyses it does not change the action of a reducing sugar on starch? (maltose). contains an enzym e? only starch. / When X is pre-trea ted at 100°C starch to maltose . This shows at high temper substance X 8/25/12 5:24 PM atures. that X is an enzyme , and is denatu red (c) Suggest two experiments that the studen an enzyme. t could perform to find out if substa nce X contain The student could s either change the pH student could use the Biuret of the reaction test to check © 2013 Marshall mixture to see if X contains protein if X is sensitiv e to pH, or the , since all enzyme of proteins. Cavendish Internatio s are made nal (Singapore) Private Limited Worksheet 4 (S)SciBioWB_04.indd 31 31 The Worksheets emphasise contextual learning, information handling and problem-solving skills. It is hoped that these emphases will stimulate students’ interest in Biology and in the application of the subject to everyday life. iv (S)SciBioWB_Frontmatter.indd iv 8/25/12 5:36 PM Contents Acknowledgements iii Preface iv Terms Used in Examination Questions vi A Guide to Essay Writing vii Worksheet 1 Cells Worksheet 2 Movement of Substances 11 Worksheet 3 Nutrients 21 Worksheet 4 Enzymes 27 Worksheet 5 Nutrition in Humans 39 Worksheet 6 Nutrition in Plants 45 Worksheet 7 Transport in Humans 57 Worksheet 8 Transport in Plants 67 Worksheet 9 Respiration in Humans 79 Worksheet 10 Coordination and Response 89 Worksheet 11 Reproduction in Plants 99 Worksheet 12 Reproduction in Humans 107 Worksheet 13 Heredity and Molecular Genetics 115 Worksheet 14 Ecology and Our Impact on the Ecosystem 125 Model Examination Paper 1 135 v (S)SciBioWB_Frontmatter.indd v 8/25/12 5:36 PM Terms Used in Examination Questions Below is a list of terms that are used in examination questions. It is important that you familiarise yourself with these terms and what is expected in your answers when such terms are encountered. Term vi What is expected in your answer Analyse Interpret data to reach conclusions. Annotate Add brief notes to a diagram, drawing or graph. Apply Use an idea, equation, principle, theory or law in a new situation. Calculate Find an answer using mathematical methods (show working unless instructed otherwise). Compare Give an account of similarities and differences between two (or more) items, referring to both (or all) of them throughout your account. Construct Represent or develop information in a graphical form (include a legend or key). Deduce Reach a conclusion from the information given. Define Give the precise meaning of a word or phrase as concisely as possible. Derive Manipulate a mathematical equation to give a new equation or result. Describe Give a detailed step-by-step account, including all relevant information. Design Produce a plan, model or simulation. Determine Find the only possible answer. Discuss Give an account including, where possible, a range of arguments, assessments of the relative importance of various factors or comparisons of alternative hypotheses. Distinguish Give the differences between two or more different items or concepts. Draw Illustrate by means of pencil lines (add labels unless told otherwise). Estimate Find an approximate value for an unknown quantity, based on the information provided and prior scientific knowledge. Evaluate Assess the consequences and limitations. Explain Give a clear account including causes, reasons or mechanisms. Identify Find an answer from a number of possibilities. List Give a sequence of names or other brief answers, with no elaboration. Each point in your sequence should be clearly separated from the rest. Measure Find a value for a quantity (include units as well). Outline Give a brief account or summary (include essential information only). Predict Give an expected result. Solve Obtain an answer using algebraic and/or other numerical methods (show working unless instructed otherwise). State Give a specific name, value or other brief answers (no supporting argument or calculation is necessary). Suggest Propose a hypothesis or an alternative answer. (S)SciBioWB_Frontmatter.indd vi 8/25/12 5:36 PM A Guide to Essay Writing Essay questions are designed to test your ability to describe and explain biological processes, principles and concepts. Marks will be awarded according to the points presented. It is important to read the essay question carefully before attempting to answer it. Do highlight key terms and words in the question. Many students misunderstand the question and as a result, their essays are inappropriate. Sometimes an essay question consists of two or more parts. Ensure that you have answered every part. Before starting to write, plan the essay. Here is how an essay may be planned: 1. 2. 3. 4. Note the topics involved. Write down all the relevant points and ideas. Organise these points and ideas so that a logical sequence is formed. Develop an introduction, followed by the sequence of points and a conclusion. While writing, keep referring to the essay plan to stay on track. Avoid using long sentences in your essay. A few examples are given below to illustrate some key points that should be taken note of while writing your essay. Example 1 Describe the functions of each of the following in the nutrition of a mammal: (a) hydrochloric acid (b) liver [2] [3] This is considered a ‘recall’ or ‘knowledge’ question. Note that the question requires a description of functions that relate to nutrition in a mammal. Care must be taken when writing so that the essay does not include items that are out of point. This is especially true for the functions of the liver. Although there are several functions of the liver listed in the Textbook, the essay must only mention the functions that relate to nutrition in mammals. Example 2 Compare nutrition in mammals with nutrition in green plants. [5] A common mistake, which most students make, is to state only the differences. An appropriate answer requires both similarities and differences to be stated. vii (S)SciBioWB_Frontmatter.indd vii 8/25/12 5:36 PM (S)SciBioWB_Frontmatter.indd viii 8/25/12 5:36 PM Class: Worksheet 1 ( Name: ) Date: Cells for Chapter 2 CONNECT IT! Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. Organ system Organ specialised cells Cell Tissue Red blood cell Xylem vessel Organism Root Cell wall (only in plants ) made up of Cytoplasm Nucleus Cell membrane which which surrounds Contains chromatin (DNA) Mitochondria hair cell which Is partially permeable and encloses the cytoplasm contains organelles such as Ribosomes Vacuoles Chloroplasts (in plant cells) Energy source for cells Synthesise proteins © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_01.indd 1 Store substances within the cell Site of photosynthesis Worksheet 1 1 8/25/12 4:46 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. For questions 1, 2 and 3, refer to the diagram shown below. 4 1 2 5 6 3 1. 2. What are the numbered parts of the cell? 1 2 3 A Cell surface membrane Cell wall Cytoplasm B Cell wall Cell surface membrane Cytoplasm C Cytoplasm Cell surface membrane Cell wall D Cytoplasm Cell wall Cell surface membrane Which structure traps light energy? A B C D 3. 3 4 5 6 1 1 2 2 Worksheet 1 (S)SciBioWB_01.indd 2 ( B ) Which structures are found in both plant and animal cells? A B C D 2 ( B ) and and and and 2 3 3 4 ( C ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:46 PM 4. The electron micrograph of a section of a liver cell taken from an animal is shown below. X What is the function of structure X? A B C D 5. ( C ) An animal cell had its nucleus removed by means of a fine glass tube. The cell was not otherwise damaged. It was put in a solution that induces cell division. It continued to survive for a day, but did not undergo cell division. An intact cell, used as a control, divided twice in that time. What can you conclude from this experiment about the role of the nucleus in the cell? A B C D 6. Contains enzymes for the breakdown of unwanted organelles Controls the synthesis of proteins Releases energy for use by the cell Transports molecules such as proteins, lipids and steroids The The The The nucleus nucleus nucleus nucleus controls the normal activity of the cell. is essential for cell division. is essential for life. is the only part of the cell that contains DNA. ( B ) The diagram below shows a three-dimensional view of a plant cell with some of its internal structures revealed. A B C D The diagram below shows a section of the cell (viewed from above) after it has been sliced along a plane. At which plane, A, B, C or D, has the cell been sliced? ( D ) © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_01.indd 3 Worksheet 1 3 8/25/12 4:46 PM 7. The diagram below shows plant cells observed under a microscope. What is the likely function of these plant cells? A B C D 8. Carry out photosynthesis Carry out respiration Provide support to the plant Transport water in the plant ( A ) The diagram below shows four types of cells that are not drawn to scale. 1 2 3 4 Which cells are involved in the transport of substances in a living organism? A B C D 4 1 and 2 1 and 4 2 and 3 3 and 4 Worksheet 1 (S)SciBioWB_01.indd 4 ( D ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:46 PM 9. Which of the following correctly relates these specialised cells to their functions? Absorption of water and mineral salts Conduction and support Transport of oxygen A Red blood cell Xylem Root hair cell B Root hair cell Xylem Red blood cell C Xylem Red blood cell Root hair cell D Xylem Root hair cell Red blood cell 10. The stomach is considered as an organ, because A B C D it it it it consists of a group of cells working together to digest food consists of different tissues working together to digest food is a basic unit of life produces digestive enzymes to digest food ( B ) . ( B ) 11. Which of the following correctly lists the organs in flowering plants? A B C D Flower, leaf, stem, root Flower, leaf, stem, root hair cell Leaf, stem, root, root hair cell Stem, root, root hair cell © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_01.indd 5 ( A ) Worksheet 1 5 8/25/12 4:46 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. The diagram below shows the structures visible in a plant cell, when it is examined under an electron microscope. H A G B F C E D (a) Identify the parts labelled A to H. A: Nucleus E: Cell wall B: Ribosome F: Cytoplasm C: Chloroplast G: Vacuole D: Cell surface membrane H: Mitochondrion (b) State one function each for A, B, G and H. A controls cell activities. B is needed to synthesise proteins in the cell. G is used to store water and food substances. H is the site of aerobic respiration. (c) What feature(s) in this cell indicate(s) that it can photosynthesise? The presence of chloroplasts (d) State one structural difference between structures D and E. D is partially permeable, while E is fully permeable. / D is made up of mainly phospholipids, while E is mainly made up of cellulose. 6 Worksheet 1 (S)SciBioWB_01.indd 6 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:46 PM 2. The diagram below shows some normal blood cells, when they are viewed under a microscope. (a) Name the type of blood cells shown, and state the function of these cells. Red blood cells To transport oxygen from the lungs to all the cells in the body (b) Explain how a red blood cell is adapted for its function. The red blood cell does not have any nucleus; instead it has haemoglobin that carries oxygen. Its biconcave shape increases surface area to volume ratio for more efficient absorption of oxygen. As it is elastic, it can squeeze through capillaries easily. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_01.indd 7 Worksheet 1 7 8/25/12 4:46 PM 3. (a) Complete the table below. Cells A B Tissue endothelial layer muscle Organ C liver (b) Arrange A, B, and C in increasing order of complexity. Least complex A 8 Worksheet 1 (S)SciBioWB_01.indd 8 Most complex B C © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:46 PM Section C: Free-Response Questions Answer the following questions. 1. Explain how the structure of a root hair cell is adapted for its function. A root hair cell absorbs water and dissolved mineral salts from the soil into the plant. It has a long, narrow extension (the root hair) that increases surface area to volume ratio for water absorption. There is also a large number of mitochondria in the cell to provide energy for active transport. 2. Compare the structural differences between a typical animal cell and a typical plant cell. The cell wall is present in a plant cell, but not in an animal cell. Chloroplasts are present in a plant cell, but not in an animal cell. A plant cell has a large central vacuole, but an animal cell has many small vacuoles. 3. The human skin is composed of many structures such as blood vessels, epidermal cells, nerves and sweat glands. Explain whether we should classify the skin as a tissue or an organ. A tissue is a group of cells that work together to perform a specific function. An organ is made up of different tissues working together to perform a specific function. Skin has structures or tissues like blood vessels, epidermal cells, nerves and sweat glands. These structures work together to contribute to the functions of skin. Therefore, the skin is an organ. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_01.indd 9 Worksheet 1 9 8/25/12 4:46 PM WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives Structured FreeResponse 1, 2, 3, 4, 5, 6 1 – 2.1 8, 9 2 1 2.2 (c) Compare the structures of typical animal and plant cells – – 2 2.1 (d) Differentiate between cell, tissue, organ and organ system 10, 11 3 3 2.2 7 – – 2.1 (a) Identify cell structures in diagrams or micrographs, and state the functions of organelles (b) State the relationship between cell structure and function (e) Examine and identify typical plant and animal cells under a microscope 10 Textbook section(s) MultipleChoice Worksheet 1 (S)SciBioWB_01.indd 10 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:46 PM Class: Worksheet 2 ( Name: ) Date: Movement of Substances Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. Movement rate affected by Diffusion Osmosis Net movement of particles from a region of higher concentration to a region of lower concentration Net movement of water molecules from a solution of higher water potential to a solution of lower water potential, across a partially permeable membrane affected by affected by Concentration gradient Swell (may burst) animal cells Turgid plant cells Cells in solution of higher water potential for Chapter 3 CONNECT IT! Surface area to volume ratio Water potential gradient across a partially permeable membrane Cells in solution of lower water potential animal cells Shrink in size e.g. red blood cells plant cells Flaccid, shrink in size Crenation Cell membrane and cytoplasm pull away from the cell wall Plasmolysis © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_02.indd 11 Worksheet 2 11 8/25/12 4:47 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. The two gas jars shown below were left for a long time to let oxygen and carbon dioxide molecules diffuse through them. oxygen molecule carbon dioxide molecule gas jar How would the molecules be arranged after a long time? A B C D ( A ) For questions 2 and 3, refer to the diagram below, which shows the appearance of a typical plant cell after it was placed in concentrated sugar solution for 15 minutes. 1 2 3 4 2. Which of the numbered structures are partially permeable? A B C D 3. ( C ) Which of the following occupies the region labelled X? A B C D 12 1 and 2 1 and 4 2 and 3 2 and 4 Air Cell sap Sugar solution Water Worksheet 2 (S)SciBioWB_02.indd 12 ( C ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:47 PM 4. The diagram shows the results of an experiment after 3 hours. The liquid in the glass tube rose to point X from initial level. X initial level Visking tubing (partially permeable) container 0.4 mol/dm3 sucrose 0.2 mol/dm3 sucrose In a second experiment, what change could be made to cause the liquid to rise higher than point X after 3 hours? A B C D 5. ( C ) Which of the following statements is true of these cells, assuming that both cells have the same thickness? A B C D Y Cell X is specially adapted for active transport, and Y for diffusion. Cell X has a greater surface area to volume ratio than Y. Cell Y is modified for absorbing materials into the cell. Diffusion of oxygen into cell Y occurs at a higher rate than X. ( D ) Dissolved gases move between the blood and body cells in our system by a certain process. What is this process known as? A B C D Absorption Diffusion Osmosis Respiration © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_02.indd 13 . diffusion filtration osmosis phagocytosis X 7. ( D ) The process by which water enters the root hair cell is called A B C D 6. A larger container with the same volume of liquid A smaller container with the same volume of liquid Change the sucrose solution in the Visking tubing to water. Change the sucrose solution in the container to water. ( B ) Worksheet 2 13 8/25/12 4:47 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. In an experiment, a peeled potato was cut into half, and wells were created as shown in the diagram below. One potato half was boiled before the experiment. Both potato halves were soaked in 10 cm3 of distilled water, before their wells were filled with 5 cm3 of 10% salt solution. peeled raw potato 10% salt solution peeled boiled potato distilled water (a) Predict what will happen to (i) the levels of the solution in the well of the peeled raw potato, and distilled water in the dish after 24 hours. The level of the solution in the well rises/increases, while the level of distilled water in the dish drops/decreases. (ii) the levels of the solution in the well of the peeled boiled potato, and distilled water in the dish after 24 hours. The level of the solution in the well drops/decreases, while the level of distilled water in the dish rises/increases. (b) Explain your answer in (a)(i) using your knowledge of diffusion and osmosis. Cells in the peeled raw potato have partially permeable cell membranes. Water potential of distilled water is greater than the water potential of cell sap, which is in turn greater than the water potential of 10% salt solution. Water moves into the cells first, then to the 10% salt solution down a water potential gradient by osmosis to achieve equilibrium. 14 Worksheet 2 (S)SciBioWB_02.indd 14 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:47 PM 2. Six identical cylinders of fresh potato (A to F), each weighing 10 g, were immersed in salt solutions of different concentrations for two hours. They were removed and reweighed. The change in mass of each cylinder was recorded in a bar graph as shown. Change in mass of potato cylinder/g 12 11 10 9 8 7 6 5 4 3 2 1 E (6) A (4) B (3) F (1) 0 –1 –2 –3 –4 –5 –6 –7 Fresh potato cylinder D (–2) C (–6) (a) Which cylinder has been immersed in salt solution with: (i) the highest concentration? (ii) the lowest concentration? C . E (iii) a concentration closest to that of the potato cells? . F . (b) Explain the changes in the cells of potato cylinder D. The potato in cylinder D was placed in a solution that had a lower water potential than the cell sap. Water moved from the cells into the solution by osmosis, down a water potential gradient (from a region of higher water potential to a region of lower water potential). Hence, the cells lost water and shrank in size, causing the mass of the potato in cylinder D to decrease. (c) Calculate the percentage increase in mass shown by potato cylinder A. Original mass of A = 10 g Change in mass of A = 4 g Percentage increase = 4 × 100% 10 = 40% © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_02.indd 15 Worksheet 2 15 8/25/12 4:47 PM (d) Suggest how the experiment can be extended to find the concentration of the potato cells. Step 1: Prepare 5 solutions with varying concentrations of salt (e.g. 10%, 5%, 2%, 1%, 0%). Step 2: Put three identical cylinders of fresh potato in each salt solution. After 30 minutes to an hour, record the change in length. Step 3: Plot the graph of change in length against concentration of salt solution. Where the line cuts through the x-axis, the value of the salt concentration will be equal to the concentration of the potato cells since there is no change in length at that point. 3. In an experiment, six potato strips of equal mass were cut and placed in separate test tubes as shown in the diagram below. Each test tube contained sugar solution of a different concentration. A B C D E F After six hours, the potato strips were removed from the test tubes and dried carefully using a paper towel. They were then weighed again. Some of the potato strips had gained in mass, while some had lost in mass. The changes in mass are shown in the table below. 16 Worksheet 2 (S)SciBioWB_02.indd 16 Test tube Amount of sugar in g in 100 cm3 of solution Change in mass in g, increase (+) or decrease (−) A 18 − 0.40 B 14 − 0.02 C 12 + 0.20 D 10 + 0.30 E 6 + 0.60 F 2 + 0.90 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:47 PM (a) Draw a graph to show the results of the experiment. Graph of change in mass of potato strips against concentration of sugar solution Change in mass/g 1.0 × 0.8 0.6 × 0.4 × 0.2 × 0 2 4 6 8 10 12 × 14 16 18 20 Concentration/g/100 cm3 −0.2 −0.4 × −0.6 (b) Which test tube contains sugar solution with the highest concentration? Explain your answer. Test tube A contains sugar solution with the highest concentration, because the decrease in mass is the largest. (c) Use the graph to estimate a value for the sugar concentration in the potato cells. 13.6 g/100 cm3 (d) Explain why the potato strip in test tube F had increased in mass. The water potential of the sugar solution in test tube F is higher than the water potential of the cell sap. Water moves from the region of higher water potential (sugar solution) to the region of lower water potential (potato cells) by osmosis. Hence, there is an increase in mass. (e) Suggest two ways to increase the accuracy and reliability of this experiment. Firstly, the difference in concentration between the sugar solutions could be decreased. Secondly, we can use more than one potato strip for each sugar concentration, and calculate the average change in mass of each strip. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_02.indd 17 Worksheet 2 17 8/25/12 4:47 PM 4. A student carried out an experiment to find out the effect of sugar concentration on chilli strips. Three chilli strips of equal length were cut and placed in Petri dishes labelled 1, 2 and 3. Each Petri dish contained different concentrations of salt solution –– 1% salt solution, 0.5% salt solution and 0.1% salt solution. The diagram below shows the appearance of the chilli strips initially and after 60 minutes. outer surface (cuticle) dish containing salt solution At the start of the experiment outer surface (cuticle) outer surface (cuticle) outer surface (cuticle) After 60 minutes dish 1 dish 2 dish 3 (a) Based on the appearance of the strips after 60 minutes, deduce the concentration of the salt solution in each of the Petri dishes. Dish 1: 1% salt solution Dish 2: 0.1% salt solution Dish 3: 0.5% salt solution (b) Explain how the change in the chilli strip in dish 2 was brought about. The salt solution in dish 2 had a higher water potential than the cell sap in the chilli strips. Water from the salt solution moved into the cells near the inner surface of the chilli strip by osmosis. Thus, the cells increased in size. However, the cells near the outer surface of the chilli strip are protected by the inelastic waterproof cuticle that does not allow water to pass through. Hence, there is differential expansion of the cells, with the cells near the outer surface not expanding as much as the cells near the inner surface. This results in the chilli strip curving towards one side. 18 Worksheet 2 (S)SciBioWB_02.indd 18 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:47 PM Section C: Free-Response Question Answer the following question. 1. Write a short paragraph to explain why the child with a bleeding leg wound, shown in the diagram, is in danger of being bitten by the piranhas. blood piranhas When the blood from the open wound enters the water, a diffusion gradient is set up. The blood molecules will move from a region of higher concentration (boy’s leg) to a region of lower concentration (near the piranhas). As the blood diffuses into the water, the piranhas will be attracted to the blood and will attack the boy. WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives (a) Define diffusion, and state its importance in nutrient uptake and gas exchange in organisms (b) Define osmosis, and describe the effects of osmosis in plant and animal tissues MultipleChoice Structured FreeResponse 1, 6, 7 1 1 2, 3, 4, 5 1, 2, 3, 4 © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_02.indd 19 Textbook section(s) 3.1 3.2 Worksheet 2 19 8/25/12 4:47 PM (S)SciBioWB_02.indd 20 8/25/12 4:47 PM Class: Worksheet 3 ( Name: ) Date: Nutrients Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. for Chapter 4 CONNECT IT! Nutrients Minerals Organic Inorganic Water Carbohydrates Fats Proteins broken down by consist of Hydrolysis Amino acids Fatty acids may be Glycerol linked by Peptide bonds forming Polypeptides Monosaccharides Disaccharides Polysaccharides formed by Fructose Maltose Cellulose Galactose Sucrose Starch Glucose Lactose Glycogen Condensation reaction formed by condensation reaction Reducing sugars (except sucrose) Food test Benedict's Brick-red solution precipitate (large amounts of reducing sugar) Food test Iodine test Blue-black coloration © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_03.indd 21 Food test Food test Ethanol emulsion test Biuret Cloudy white emulsion and heat is given off coloration test Violet Worksheet 3 21 8/25/12 4:48 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. Which statement correctly describes one of the roles of water in the human body? A B C D 2. ( A ) Which form of carbohydrate is usually stored in the human body? A B C D 3. As a solvent to dissolve some substances As a source of energy As an insulating layer for the body As material for the build-up of muscles Glucose Glycogen Glycerol Starch ( B ) The diagram below shows the breakdown of three types of organic molecules. X Y Z What are the identities of X, Y, and Z? 22 X Y Z A Carbohydrate Fat Protein B Carbohydrate Protein Fat C Protein Carbohydrate Fat D Protein Fat Carbohydrate Worksheet 3 (S)SciBioWB_03.indd 22 ( B ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:48 PM 4. A student was given three test tubes, each containing two food substances. The student was asked to identify the food substances present in each test tube. The table below shows the results of the student’s tests. Reagent added Test tube Biuret solution Benedict’s solution Iodine solution X Solution remains blue Orange-red precipitate formed Blue-black colour observed Y Violet colour observed Orange-red precipitate formed Solution remains brown Z Violet colour observed Solution remains blue Blue-black colour observed Which conclusion is consistent with the results? A B C D 5. Egg white and maltose are present in test tube X. Egg white and starch are present in test tube X. Egg white and starch are present in test tube Z. Starch and maltose are present in test tube Y. ( C ) Food tests are carried out on four solutions. Which solution contains only glucose and proteins? ( indicates a positive reaction; indicates negative result.) Solution Benedict’s test Biuret test Ethanol emulsion test A B C D © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_03.indd 23 ( C ) Worksheet 3 23 8/25/12 4:48 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. 2. A student conducted food tests on a solution given to him. Complete the table to show the conclusions which can be drawn from the tests. Food test Observation Conclusion Sample mixed with Benedict’s solution and boiled Orange-red precipitate formed Reducing sugar is present. Sample mixed with sodium hydroxide and 1% copper sulfate, drop by drop Violet colour observed Protein is present. Sample mixed with iodine solution Blue-black colour observed Starch is present. Sample mixed with ethanol and then water White emulsion formed Fat is present. (a) Name the type of reaction by which amino acids are linked together. Condensation (b) Name the product formed when many amino acids are linked together. Polypeptide 3. The table below refers to three organic molecules. Complete the table. Carbohydrates Elements present in the molecule Basic units Type of reaction to convert complex form to simpler form Two functions of the molecules in a living organism 24 Worksheet 3 (S)SciBioWB_03.indd 24 C, H, O Proteins C, H, O, C, N H, O, N maybe P S S maybeand P and Monosaccharides Amino acids Hydrolysis Hydrolysis Any two of the following: Energy source / Production of mucus / Constituent of cellulose cell wall Any two of the following: Synthesis of enzymes / Production of hormones / Production of antibodies / Growth and repair Fats C, H, O Fatty acids and glycerol Hydrolysis Any two of the following: Energy source / Solvent for fat-soluble vitamins / Insulating layer © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:48 PM Section C: Free-Response Question Answer the following question. 1. David is given a slice of cucumber and a slice of tomato. He is curious to find out if the food samples contain reducing sugars, proteins or fats. Outline the experiments that he should conduct to test the food samples for reducing sugars, proteins and fats. 1. Cut the cucumber into small pieces. Divide the cucumber pieces into three portions. 2. Add 1 cm3 of distilled water to each portion. Mix well and decant. 3. Do the following tests: (i) Reducing sugar test — Benedict’s test + observations (ii) Protein test — Biuret test + observations (iii) Fat test — Ethanol emulsion test + observations 4. Repeat for tomato. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_03.indd 25 Worksheet 3 25 8/25/12 4:48 PM WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives (a) Explain the importance of water in living organisms (b) Describe how glycogen is formed from simpler glucose molecules 26 MultipleChoice Structured FreeResponse 1 Textbook section(s) 4.2 2, 3 3 4.3 (c) Describe how fats are formed from glycerol and fatty acids 3 3 4.4 (d) Describe how proteins and polypeptides are formed from amino acids 3 2, 3 4.5 (e) Carry out experiments to test for the presence of reducing sugars, starch, fats and proteins 4, 5 1 Worksheet 3 (S)SciBioWB_03.indd 26 1 4.3, 4.4, 4.5 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:48 PM Class: Worksheet 4 ( Name: ) Date: Enzymes Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. Enzymes Functions Build up complex substances Break down complex substances are Biological catalysts Mode of action Characteristics Speed up reactions Lower activation energy small Only amount needed Lock and key hypothesis mainly made of for Chapter 5 CONNECT IT! Proteins Denaturation occurs at extremes of temperature or pH Class based on type of reaction catalysed e.g. Hydrolases Enzymes are specific Work best at optimum temperature and optimum pH Substrate fits into enzyme’s active site forms Enzyme-substrate Catalyse reversible reactions complex forms Product once formed, product leaves Enzyme molecule free to take part in another reaction Temperature affected by pH © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_04.indd 27 Worksheet 4 27 8/25/12 5:24 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. Enzymes are A B C D 2. proteins that are used up in chemical reactions proteins that catalyse chemical reactions at any temperature proteins that catalyse chemical reactions in living organisms proteins that speed up the breakdown of complex compounds into simpler substances ( C ) Enzymes are added to biological washing powders to remove stains. Which would be the most effective enzymes in removing chocolate milk stains? A B C D 3. . Amylase and catalase Amylase and maltase Protease and catalase Protease and lipase ( D ) The diagram shows an experiment that was carried out in water at different temperatures. water mixture of amylase and starch in a Visking tubing The water was tested for the presence of reducing sugar at 30-second intervals. At which temperature would reducing sugar be detected most rapidly? A B C D 28 10°C 20°C 40°C 100°C Worksheet 4 (S)SciBioWB_04.indd 28 ( C ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:24 PM 4. Which characteristic of enzymes is the diagram below trying to illustrate? + + enzyme subtrate A complex X enzyme + product Y product Z + enzyme A B C D 5. subtrate B Enzymes are specific in nature. Enzymes speed up chemical reactions. Enzymes work best at optimum pH. Enzymes work best at optimum temperature. In the ‘lock and key’ hypothesis, what is the ‘lock’, and what is the ‘key’? Lock Key A Enzyme Substrate B Substrate Enzyme C Product Enzyme D Enzyme Product © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_04.indd 29 ( A ) ( A ) Worksheet 4 29 8/25/12 5:24 PM 6. Which graph correctly shows the effect of pH on the rate of reaction of an enzyme-catalysed reaction? A B Rate of reaction Rate of reaction pH pH C D Rate of reaction Rate of reaction pH pH ( B ) 7. Curve X represents an enzyme-catalysed reaction under optimum conditions. Curve Y shows the action of the same enzyme on the same substrate, but with one alteration to the reaction condition. Substrate concentration/% 100 curve Y 50 curve X Time/min Which of the following factors could give the results shown by curve Y? A B C D 30 A 10°C decrease in temperature A decreased substrate concentration An increased enzyme concentration An increased substrate concentration Worksheet 4 (S)SciBioWB_04.indd 30 ( A ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:24 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. X is an unknown substance extracted from a living organism. A student was required to investigate the ability of X to bring about the hydrolysis of starch. She set up two water baths, one containing boiling water (100°C) and the other containing water at 36°C. The student used the water baths and set up the reaction mixtures as follows: • Test tube 1: 1 cm3 of X (pre-treated at 100°C for 1 minute) + 9 cm3 of distilled water • Test tube 2: 1 cm3 of X (pre-treated at 36°C for 1 minute) + 9 cm3 of distilled water • Test tube 3: 1 cm3 of X (pre-treated at 100°C for 1 minute) + 9 cm3 of starch • Test tube 4: 1 cm3 of X (pre-treated at 36°C for 1 minute) + 9 cm3 of starch • Test tube 5: 1 cm3 of distilled water + 9 cm3 of starch The reaction mixtures were placed in the water bath that contained water at 36°C. After 15 minutes, the student tested the mixture in each test tube for the presence of reducing sugar using Benedict’s test. Her observations were as follows: Test tube Observation for Benedict's test 1 Mixture remained blue 2 Mixture remained blue 3 Mixture remained blue 4 Mixture turned from blue to orange-red 5 Mixture remained blue (a) Based on the student's observations, what is the action of substance X on starch? X breaks down starch (a non-reducing sugar) into a reducing sugar (maltose). (b) What evidence supports that substance X contains an enzyme? X is specific in action. That is, it hydrolyses only starch. / When X is pre-treated at 100°C (test tube 3), it does not change starch to maltose. This shows that X is an enzyme, and is denatured at high temperatures. (c) Suggest two experiments that the student could perform to find out if substance X contains an enzyme. The student could either change the pH of the reaction mixture to see if X is sensitive to pH, or the student could use the Biuret test to check if X contains protein, since all enzymes are made of proteins. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_04.indd 31 Worksheet 4 31 8/25/12 5:24 PM (d) What is the purpose of including test tubes 1 and 2? Why are they necessary? Test tubes 1 and 2 act as controls. Both test tubes show that X (an exract from a living organism) does not originally contain reducing sugar. Hence, when starch is added into test tube 4, and the content is eventually tested positive for reducing sugar, it shows that the reducing sugar is due to the hydrolysis of starch by X at 36°C. 2. An experiment was conducted to investigate the action of the enzyme amylase in solutions of different pH. Eight test tubes were set up, each filled with 5 cm3 of buffered solutions of pH 3, 4, 5, 6, 7, 8, 9 and 10 respectively. 5 cm3 of amylase and 30 cm3 of starch were then added to each test tube, in sequence. 1 cm3 of the mixture was taken out every 30 seconds, and tested for starch using the iodine test, until it gave a negative starch test. The time taken to digest all the starch was recorded. The results are represented in the graph below. Time taken to convert all the starch/min 1 2 3 4 5 6 7 8 9 10 pH (a) What variable (factor) was being tested in this experiment? It was the pH of the reaction. (b) Name two conditions that had to be kept constant. The volume of reagents and temperature had to be kept constant. (c) From the results, what is the optimum pH of amylase? Explain your answer. It is pH 7. This is because, amylase took the shortest time to convert the starch to maltose at pH 7. (d) Sketch on the diagram below, how the amount of substrate varies during the reaction at optimum conditions. Amount of substrate left in reaction/ arbitrary unit 1 2 3 4 5 6 7 8 9 10 32 Worksheet 4 (S)SciBWB_04_CS6.indd 32 Time taken/min © 2013 Marshall Cavendish International (Singapore) Private Limited 11/10/16 6:11 PM 3. A dish is filled with milk agar made by mixing milk with agar powder. Four holes are cut in the milk agar, and each hole is filled with the substances shown. A distilled water B D milk agar protease solution C protease solution + concentrated sodium hydroxide solution boiled protease solution (a) Write a word equation to show the effect of protease on milk proteins. milk proteins protease polypeptide/peptide/amino acids (b) After one day of incubation at 37°C, the agar plate is put under a light to observe for clear zones around the holes. (i) Why should one look for clear zones around the holes? If the enzyme digested the milk proteins in the agar, it will turn the cloudy areas into a clear zone. (ii) State what you would observe in each hole. Hole A: Clear zone seen Hole B: No clear zone seen Hole C: No clear zone seen Hole D: No clear zone seen (c) Explain the purpose of the distilled water in hole D. The distilled water in hole D is used as a control to show that it is the protease that digests milk proteins to polypetide/peptide/amino acids. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_04_CS6.indd 33 Worksheet 4 33 11/10/16 6:11 PM 4. The enzyme catalase catalyses the breakdown of hydrogen peroxide to water and oxygen. An experiment was set up to find the initial rate of reaction of catalase. The volume of oxygen is measured at every 30-second interval. The results are shown below. Time/s 30 60 90 120 150 180 210 240 270 300 Gas volume collected/cm3 6 12 16 19 22 23 24 25 25.5 26 (a) Explain the meaning of 'catalyse'. It means to speed up the rate of a chemical reaction. (b) Plot a graph of the results obtained. Graph of volume of gas collected against time × × × (110, 24) × × Gas volume collected/ 25 cm3 × 20 × × 15 × 10 × 5 (15, 5) 0 50 100 150 200 250 300 Time/s (c) From the graph, calculate the initial rate of reaction. Show your working clearly. Initial rate of reaction = Y1 – Y0 X1 – X0 24 – 5 = 110 – 15 19 1 = 95 = 5 = 0.2 cm3/s 34 Worksheet 4 (S)SciBioWB_04.indd 34 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:24 PM (d) Name two conditions or factors that had to be kept constant in this experiment. Any two of the following: Temperature / pH / Amount of enzyme used LEARN & APPLY Go through the worked example below, then try to answer the Free-Response Questions. Worked Example Explain the importance of enzymes in plants. Thought Process Giving examples will help to answer the questions. It is important to take note that the question is asking for plants. Answer Points to cover 1. Enzymes help to speed up chemical reactions in plants. 2. Without enzymes, many reactions in plants will take a long time to complete. 3. For example, photosynthesis and respiration require enzymes to speed up these reactions. 4. Enzymes are also important in seed germination. Enzymes will digest the stored starch into glucose for the embryo to germinate and grow. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_04_CS6.indd 35 Worksheet 4 35 11/10/16 6:11 PM Section C: Free-Response Questions Answer the following questions. 1. Explain, with reasons and examples, how the biological activity of an enzyme may be affected by: (a) changes in pH; and (b) increasing temperatures. (a) Enzymes are sensitive to the acidity or alkalinity of a solution. Some work best in acidic conditions, for example, pepsin in the stomach, whereas others work best in alkaline conditions, for example, pancreatic amylase. Extreme changes in pH denature the enzyme which is protein in nature. When an enzyme is denatured, it loses its active site shape, and the substrate can no longer fit into it. So its biological activity decreases. (b) Enzymes work best under optimum temperature. A high temperature increases the kinetic energy supplied to the reacting molecules, which then increases the rate of effective collisions between substrates and enzymes. The rate of the reaction increases up to the optimum temperature. Beyond the optimum temperature, an enzyme, being protein in nature, will be denatured, and the active site will lose its shape. The rate of the biological reaction will, therefore, decrease. 2. (a) Describe the major properties of enzymes. (b) Discuss the mode of action of an enzyme in terms of active site, enzyme-substrate complex and enzyme specificity. (a) Enzymes are highly specific in action. They are mainly made up of proteins. Enzymes speed up chemical reactions without themselves being changed at the end of the reaction. Small amounts of enzymes are capable of catalysing a large number of reactions. Enzymes work within a narrow range of conditions. For instance, enzymes have an optimum working temperature, and beyond a certain temperature, they are denatured. Enzymes are also affected by the acidity or alkalinity of the solutions in which they act. (b) An enzyme acts on a substrate and converts it into a product. The binding of a substrate to the active site of an enzyme is specific in nature. A substrate fits into an enzyme’s active site, like how a key fits into a lock, forming an enzyme-substrate complex. The active site on the enzyme is a region on the protein with a specific shape where the substrate binds. 36 Worksheet 4 (S)SciBWB_04_CS6.indd 36 © 2013 Marshall Cavendish International (Singapore) Private Limited 11/10/16 6:12 PM WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives MultipleChoice Textbook section(s) (a) Define an enzyme 1 5.1 (b) State the function of enzymes 1 5.1 (c) Explain how enzymes are classified 2 3 4, 5, 7 1, 3, 5 (d) Describe the characteristics of enzymes (e) Describe the mode of enzyme action based on the ‘lock-and-key’ hypothesis 5 (f) Explain how conditions such as temperature and pH can affect the rate of an enzyme-catalysed reaction 3, 6, 7 © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_04.indd 37 Structured FreeResponse 1, 2, 4 5.1 2 5.2 2 5.2 1 5.2 Worksheet 4 37 8/25/12 5:24 PM (S)SciBioWB_04.indd 38 8/25/12 5:24 PM Class: Worksheet 5 ( Name: ) Date: Nutrition in Humans for Chapter 6 CONNECT IT! Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. Nutrition consists of Feeding Digestion in movement along Alimentary canal Teeth Salivary Buccal cavity (mouth) glands Absorption Assimilation Peristalsis rhythmic, wave-like muscular contractions of the gut wall Salivary secrete amylase Tongue Pharynx Amylase Maltase Protease produce enzymes contains intestinal cells Oesophagus Stomach Lipase Hydrochloric acid Duodenum secretes Ileum Gastric juice contains Small intestine Pepsin Jejunum Large intestine Organs and glands associated with the gut adapted for absorption Rectum Liver Gall bladder Anus secretes stored in Capillaries Villi Folds Egestion join to form Hepatic portal vein transports sugars to © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_05.indd 39 Pancreas Pancreatic juice secretes Bile Glucagon deamination of amino acids Urea Insulin Worksheet 5 39 8/25/12 4:51 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. Carbohydrate digestion occurs mainly in the A B C D 2. ( B ) Hepatic artery Hepatic portal vein Hepatic vein Pulmonary artery ( B ) ileum large intestine liver stomach ( C ) What happens to alcohol after it enters the human body? A B C D 40 To break down fat into fatty acids and glycerol To emulsify fat into minute fat globules To transport the digested fat into the bloodstream To transport the digested fat into the lacteals Excess amino acids are toxic to the human body, and must be deaminated. Excess amino acids are . deaminated in the A B C D 5. ( A ) Which blood vessel transports absorbed food substances to the liver? A B C D 4. mouth and duodenum mouth and stomach stomach and duodenum stomach and ileum What is the function of bile? A B C D 3. . Alcohol is absorbed by the stomach. Alcohol is deaminated by the liver. Alcohol is detoxified by the liver. Alcohol is passed out as urine. Worksheet 5 (S)SciBioWB_05.indd 40 ( C ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:51 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. Complete the table below, which illustrates the process of digestion. Region of digestion Active enzyme Substrate Product(s) Mouth Amylase Starch Maltose Stomach Pepsin Proteins Polypeptides Maltase Maltose Glucose Peptidase Polypeptides Amino acids Lipase Fats Fatty acids and glycerol Duodenum 2. The diagram shows part of the human digestive system. D A E B C (a) Identify A. The stomach (b) What are the functions of parts B, C and E during digestion? B: Produces pancreatic enzymes C: Site of completion of digestion / absorption of digested products E: Storage of bile (c) D produces a substance that is important for fat digestion. State what this substance is, and its role in fat digestion. D (the liver) produces bile. Bile emulsifies fats, facilitating the action of lipase. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_05.indd 41 Worksheet 5 41 8/25/12 4:51 PM (d) (i) State the blood vessel that carries blood from C to D. Hepatic portal vein (ii) State two end-products of digestion carried by this blood vessel. Amino acids and glucose Section C: Free-Response Questions Answer the following questions. 1. A patient suffering from gall bladder disease has his gall bladder removed. Suggest and explain the type of diet and lifestyle that he should adopt after his gall bladder is removed. The patient should eat smaller and more frequent meals because when the gall bladder is removed, bile is no longer being stored but drains continuously into the intestine. He should also avoid fatty food because there is less bile to emulsify the fats into smaller fat globules. A high-fat diet will not be digested properly and will result in diarrhoea or constipation. 2. Hepatitis B is a viral infection of the liver. Patients suffering from severe Hepatitis B infection may die eventually due to liver failure. Explain, using your knowledge of the liver, why patients suffering from Hepatitis B may die from liver failure. Liver has many functions. Examples include: – Regulation of blood glucose levels – Conversion of excess glucose to glycogen, when blood sugar levels are higher than usual, and conversion of glycogen to glucose, when blood sugar levels are low – Regulation of the amount of amino acids in the body – Deamination of excess amino acids A number of body functions are affected when the patient suffers from liver failure. This eventually leads to death. 42 Worksheet 5 (S)SciBWB_05_CS6.indd 42 © 2013 Marshall Cavendish International (Singapore) Private Limited 2/12/16 9:58 am WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives (a) Describe the functions of the main regions of the alimentary canal and the associated organs, in relation to ingestion, digestion, absorption, assimilation and egestion of food MultipleChoice Structured FreeResponse 1 2 1 (b) Describe the functions of enzymes such as amylase, maltase, protease and lipase in digestion, listing the substrates and end-products (c) State the function of the hepatic portal vein (d) State the role that the liver plays in human nutrition (e) State the role of the liver in the breakdown of alcohol 1 5 © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_05_CS6.indd 43 6.1, 6.2 6.2 3 2, 4 Textbook section(s) 6.4 2 6.5 6.5 Worksheet 5 43 11/10/16 6:13 PM (S)SciBioWB_05.indd 44 8/25/12 4:51 PM Class: ) Worksheet 6 ( Name: Date: Nutrition in Plants Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. for Chapter 7 CONNECT IT! Photosynthesis Light intensity raw materials limiting factors CO2 Carbon dioxide concentration affect affect Temperature , Sunlight , Reactions in chloroplasts Water Rate of photosynthesis Equation Carbon dioxide + Water chlorophyll light energy Oxygen + Glucose excess glucose converted to Sucrose for Transport converted and stored as Starch © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_06_CS6.indd 45 Worksheet 6 45 11/10/16 6:14 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. 2. The equation which best describes photosynthesis in words is A carbon dioxide B glucose + oxygen C glucose + oxygen + water D water + carbon dioxide . glucose + oxygen + water water + carbon dioxide carbon dioxide glucose + oxygen ( D ) Which of the following cells in a leaf do not contain chloroplasts? A B C D Guard cells Palisade mesophyll cells Spongy mesophyll cells Upper epidermal cells ( D ) For questions 3 and 4, refer to the diagram below which shows a section from the leaf of a green plant. 1 2 3 4 vascular bundle 5 6 3. 46 Which regions contain cells with the highest and lowest number of chloroplasts? Region with highest number of chloroplasts Region with lowest number of chloroplasts A 1 2 B 2 6 C 5 2 D 6 1 Worksheet 6 (S)SciBioWB_06.indd 46 ( B ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:53 PM 4. After a period of bright light, which regions contain the most starch? A B C D 5. 1 and 6 2 and 4 2 and 5 3 and 5 ( C ) The photo below shows part of a leaf. What is X? X A B C D 6. Guard cell Palisade mesophyll Spongy mesophyll Stoma ( D ) How does most of the carbon dioxide enter the photosynthesising cells of a leaf? A B C D Diffusion through the Diffusion through the Diffusion through the Diffusion through the epidermis of the leaf phloem of the leaf stomata of the leaf xylem of the leaf © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_06.indd 47 ( C ) Worksheet 6 47 8/25/12 4:53 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. The equation for photosynthesis is usually given as: Water + Carbon dioxide light energy chlorophyll Glucose + Oxygen Which organelle does photosynthesis occur in? Chloroplast 2. The graph shows the rate of photosynthesis of a plant with increasing light intensity. The temperature is kept at 20°C. Rate of photosynthesis Light intensity (a) Describe the relationship between light intensity and the rate of photosynthesis. The rate of photosynthesis increases as light intensity increases, until a certain value where it levels off. (b) Draw a line on the graph to show the rate of photosynthesis against light intensity, when the temperature is increased to 30°C. 48 Worksheet 6 (S)SciBioWB_06.indd 48 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:53 PM 3. The diagram shows a cross-section of a dicotyledonous leaf when viewed under a microscope. A C D B E F (a) Identify the parts labelled A to F. A: Palisade mesophyll D: Phloem B: Spongy mesophyll E: Intercellular air space C: Xylem F: Stoma (b) State one structural difference between the cells found in A and epidermal cells. Unlike the cells in A, epidermal cells do not have chloroplasts. (c) State one function of part C. To conduct water / To provide mechanical support to the plant (d) Parts B and F are involved in a similar function. Describe this function, and state its importance to the plant. They provide spaces for the exchange of carbon dioxide and oxygen. These gases must be able to move in and out of the cells so that respiration and photosynthesis may take place. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_06.indd 49 Worksheet 6 49 8/25/12 4:53 PM 4. The apparatus below can be used to investigate the rate of photosynthesis. air bubble plastic tube graduated capillary tube well-lubricated syringe cork dilute sodium hydrogencarbonate solution water at room temperature water plant boiling tube (a) Suggest what is measured in this experiment to show that photosynthesis has occurred. The volume of oxygen given off during photosynthesis is being measured. (b) Describe how this apparatus can be used to measure the effect of light intensity on the rate of photosynthesis. Step 1: Shine light on the water plant at varying distances. (Distance between light source and plant affects the intensity of light falling on the plant.) Step 2: Use a graduated syringe to measure the volume of oxygen given off and collected at specific time intervals for each distance at which the light source is placed. Step 3: Calculate rate of oxygen given off at each distance (light intensity) to study the effect of light intensity on the rate of photosynthesis. (c) State three steps that need to be taken during the experiment described in (b) to minimise sources of error. Any three of the following: Some time should be allowed for the water plant to adapt to new conditions before taking readings. / The water plant should be as upright as possible to allow maximum light exposure. / The cut end of the plant should be positioned upwards. / The light source cannot be too near the water plant as it will heat the plant and may cause enzymes to be denatured. / The whole set-up should be air-tight so that the volume of oxygen measured comes from the water plant only. 50 Worksheet 6 (S)SciBioWB_06.indd 50 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:53 PM 5. A shoot of Hydrilla was placed in a boiling tube containing pond water. A bench lamp with a 60 W bulb, shining at the boiling tube, was placed 25 cm away. An oxygen probe was put into the boiling tube, and the oxygen concentration was recorded using a data logger. The oxygen concentration was recorded after 5 minutes, and again at 10 minutes. The experiment was then repeated using the same Hydrilla in the same boiling tube, but with the lamp placed 5 cm away from the boiling tube. The results are given in the table below. Distance of lamp/cm Oxygen concentration after 5 mins/% saturation Oxygen concentration after 10 mins/% saturation 25 60 65 5 64 84 (a) Draw the experimental set-up as described above. oxygen sensor thermometer interface computer boiling tube light intensity sensor beaker pond water lamp Hydrilla retort stand water bath (to keep temperature constant) tripod stand 25 cm © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_06.indd 51 Worksheet 6 51 8/25/12 4:53 PM (b) Based on the results of this experiment, explain the effect of light intensity on oxygen production. As light intensity increases when the lamp is moved nearer to the plant, more oxygen is produced. This is due to the increased rate of photosynthesis in the plant under increased light intensity. (c) (i) What process are we trying to investigate when we measure the oxygen concentration in this experiment? Photosynthesis (ii) Why is the measurement of oxygen concentration considered an inaccurate measurement of the rate of the process stated in (c)(i)? Oxygen is a by-product of photosynthesis. Some of the oxygen produced by photosynthesis, may be used by the plant for respiration. Hence, the volume of oxygen measured here will be less than the actual volume produced during photosynthesis. (d) State one environmental condition that should be kept constant during this experiment. Describe how this condition can be kept constant. Any of the following: Concentration of carbon dioxide. This can be done by adding a known concentration of sodium hydrogencarbonate into the water. / Temperature. This can be done by using a water bath. The temperature of the water in the set-up can be measured using a thermometer and adjusted by adding hot or cold water if needed. 52 Worksheet 6 (S)SciBioWB_06.indd 52 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:53 PM 6. Photosynthesis takes place mostly in the leaves of plants. The laminae of most leaves are wide and thin, as shown in the diagram below. (a) Name the cells in the leaf where most photosynthesis takes place. Mesophyll cells (b) Which tissue in the leaf supplies water to the cells for photosynthesis? Xylem (c) Explain how having a wide lamina helps a leaf to photosynthesise. Having a wide lamina means that the leaf has more surface area for light absorption. Light energy is needed for photosynthesis. (d) Explain how having a thin lamina helps a leaf to photosynthesise. Having a thin lamina means that gases such as carbon dioxide, which is one of the raw materials for photosynthesis, can diffuse easily into the mesophyll cells, since there is a shorter distance to travel. It is also easier for light to penetrate the leaf to reach all the photosynthetic cells. (e) Most leaves are covered with a transparent, waterproof cuticle. How does this feature aid in photosynthesis? The waterproof cuticle helps prevent excessive water loss by evaporation from the leaf surface. This ensures that there is enough water for photosynthesis. It also helps to focus the light rays onto the mesophyll layer. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_06.indd 53 Worksheet 6 53 8/25/12 4:53 PM Section C: Free-Response Questions Answer the following questions. 1. Discuss the major adaptations of the leaf for photosynthesis. The petiole holds the leaf lamina away from the stem, so that the lamina can absorb sufficient sunlight and air. The leaf lamina has a large flat surface compared to its volume. This enables the leaf to absorb the maximum amount of sunlight for photosynthesis. A thin, wide lamina ensures that carbon dioxide, the raw material for photosynthesis, can rapidly reach the inner cells of the leaf. A network of veins extends throughout the leaf. Veins are made of the vascular tissues, xylem and phloem. Xylem transports water and mineral salts to the leaf cells. Phloem transports sugars away from the leaf cells to other parts of the plant. This maintains a concentration gradient, so that the photosynthetic reactions proceed in the forward direction. A waxy cuticle on the leaf epidermis protects the leaf by reducing the evaporation of water. Water is an essential raw material for photosynthesis. Lack of water would also cause the leaf to wilt, reducing surface area for light absorption. Stomata on the lower epidermis open in sunlight, allowing carbon dioxide to diffuse in and oxygen to diffuse out. Mesophyll cells of the leaf contain numerous chloroplasts. Chloroplasts contain chlorophyll, which absorbs light energy for photosynthesis. Mesophyll cells in the upper regions of the leaf (palisade mesophyll cells), contain more chloroplasts than the spongy mesophyll cells, since more light energy can be absorbed near the leaf surface. An interconnecting system of air spaces in the lower regions of the leaf (spongy mesophyll) allow rapid diffusion of carbon dioxide into mesophyll cells. 54 Worksheet 6 (S)SciBioWB_06.indd 54 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:53 PM 2. (a) Describe an experiment to show the effect of temperature on the rate of photosynthesis. Step 1: Submerge a water plant like Hydrilla, with a length of 5 cm, in a boiling tube filled with dilute sodium hydrogencarbonate solution. Ensure that the cut end of the water plant is positioned upwards. Place this boiling tube in a water bath. Place a thermometer in the water bath to check that the temperature is kept constant. Step 2: Place a lamp with a 60 W bulb, 10 cm from the plant. Keep this distance constant throughout the experiment. Step 3: Add ice-cubes or cold water to the water bath to adjust the temperature to 5°C. Allow some time for the plant to adapt to the conditions provided, before taking readings. Step 4: Count the number of bubbles produced by the plant over a period of 5 minutes. Repeat this a few times to obtain an average rate. Step 5: Repeat step 4 at different temperatures, e.g. 15°C, 25°C, 35°C, 45°C, 55°C, 65°C and 75°C. Step 6: Record the results in a table. Plot a graph to show the rate of bubbling per minute against the temperature. (b) Discuss why the following statement is true: “Temperature will only increase the rate of photosynthesis to a certain extent. At very high temperatures, the rate of photosynthesis will decrease.” Photosynthesis is an enzyme-catalysed reaction. There will be increased collisions of enzyme and substrate molecules, as temperature increases. This results in more enzyme-substrate complexes being formed, and thus, an increased rate of reaction. However, at very high temperatures, enzymes being protein in nature, will be denatured. The substrate no longer fits into the active site, and an enzyme-substrate complex cannot be formed. Thus, the rate of photosynthesis will decrease. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_06.indd 55 Worksheet 6 55 8/25/12 4:53 PM WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives MultipleChoice (a) State that chlorophyll traps light energy, and converts it into chemical energy for the formation of carbohydrates (b) State the equation, in words, for photosynthesis 1 (c) Investigate and state the factors affecting the rate of photosynthesis 7.1 1 7.1 2, 4, 5 5 3 (e) Relate how the structure of the leaf is adapted to its functions 2, 3, 4 1, 6 Worksheet 6 (S)SciBioWB_06.indd 56 6 Textbook section(s) 1 (d) Identify and label the various cellular and tissue structures of a dicotyledonous leaf, as seen under the microscope (f) Outline the intake of carbon dioxide and water by plants 56 Structured FreeResponse 2 7.1 7.2 1 7.2 7.2 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 4:53 PM Class: Worksheet 7 ( Name: ) Date: Transport in Humans for Chapter 8 CONNECT IT! Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. Circulatory system Blood consists of Components Red blood cells White blood cells contain for Haemoglobin Blood clotting Lymphocytes Phagocytes binds to oxygen Plasma Platelets Phagocytosis fibrin threads entangle blood cells to form a mass (clot) in spaces between tissue cells and blood capillaries contains Tissue fluid Antibodies produce Blood vessels Arteries branch into Arterioles branch into Capillaries join to form Venules join to form Veins e.g. contain Coronary arteries Internal valves bring nutrients and oxygen to two upper chambers Atria two lower chambers Ventricles (singular: atrium ) Heart © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_07.indd 57 Worksheet 7 57 8/25/12 5:01 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. After centrifugation, blood separates into three layers as shown in the diagram below. Layer X is the topmost layer and is yellowish and translucent. Layer Y is the bottom layer and is dark red in colour. X Y What are the functions of the components of blood in layers X and Y? 2. Layer X Layer Y A Defend the body against foreign substances Transport nutrients and waste materials B Transport nutrients and waste materials Transport oxygen C Transport oxygen Defend the body against foreign substances D Transport oxygen Transport nutrients and waste materials In which of the following regions will the highest pressure develop? A B C D 3. Left atrium Left ventricle Pulmonary artery Pulmonary vein ( B ) A person living at a high altitude, where there is low oxygen concentration, will adapt physiologically by . A B C D 58 ( B ) decreasing the number of red blood cells feeling dizzy and nauseous increasing his breathing rate increasing the number of red blood cells Worksheet 7 (S)SciBWB_07_CS6.indd 58 ( D ) © 2013 Marshall Cavendish International (Singapore) Private Limited 11/10/16 6:15 PM 4. The diagram below shows cross-sections of two types of blood vessels. (The blood vessels are not drawn to scale.) P Q What is the identity of P and Q? 5. P Q A Artery Capillary B Artery Vein C Capillary Artery D Capillary Vein What causes the 'lub' and 'dub' sounds of a heartbeat? A B C D 6. ( C ) The The The The backflow of blood hitting the heart muscles closure of the semi-lunar valves, bicuspid valve and tricuspid valve flow of blood into the pulmonary vein and aorta return of blood into the heart from the venae cavae and pulmonary artery ( B ) The diagram below shows a section of the human heart. 4 3 1 2 Which of the numbered part(s) of the heart carries/carry oxygenated blood? A B C D 1 4 1 3 only only and 2 only and 4 only © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_07.indd 59 ( C ) Worksheet 7 59 8/25/12 5:01 PM 7. After a patient underwent heart surgery, there was an increase in the number of white blood cells in his body. What could be a possible reason for this increase? A B C D The patient might have developed an infection after the surgery. The patient might need more nutrients to be transported around his body. The patient might need more oxygen to help him recover faster. The patient might need to accelerate the clotting of his blood to prevent excessive blood loss from the surgery. ( A ) Section B: Structured Questions Answer the following questions in the spaces provided. 1. The diagram represents some blood cells. X Y Z (a) Name the cells labelled X and Y. X: Red blood cell Y: White blood cell (b) Z is involved in blood clotting. Name Z. Platelet (c) State one function of X and Y. X: To transport oxygen from the lungs to all the parts of the body Y: Phagocytosis 60 Worksheet 7 (S)SciBioWB_07.indd 60 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:01 PM 2. The graph shows the blood pressure in the aorta and left ventricle during two cardiac cycles. Pressure/ mm of Hg pressure in the aorta pressure in the left ventricle 0 0.5 1.0 1.5 Time/s (a) How long is a cardiac cycle? 1.12 second – 0.32 second = 0.80 second (b) State the duration in each cardiac cycle when the aortic valve (semi-lunar valve) is closed. Explain how you arrived at your answer. 1.02 second – 0.22 second = 0.80 second The aortic valve remains closed throughout the interval where the aortic pressure is above ventricular pressure. (c) If a line indicating pressure in the right ventricle is drawn on this graph, state one similarity and one difference it would have with the existing line indicating pressure in the left ventricle. The pattern of the two lines, and the shape of the graphs would be the same. The pressure in the right ventricle would be lower than that in the left ventricle. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_07.indd 61 Worksheet 7 61 9/6/12 10:07 AM 3. (a) Complete the diagram by drawing in the path of blood into and out of the heart. (i) Use a black/blue pen to show the path of deoxygenated blood into and out of the heart. (ii) Use a red pen to show the path of oxygenated blood into and out of the heart. Key oxygenated blood N M deoxygenated blood B A (b) Name valves M and N. M: Semilunar valve / Aortic valve N: Bicuspid valve (c) Which side, A or B, is the left side of the heart? Explain your answer. B is the left side, because the muscular wall is thicker than that of side A. (d) When the left ventricle is full and the muscles contract to force the blood into the aorta, valve N closes. Why does valve N close? To prevent the backflow of blood into the atrium 62 Worksheet 7 (S)SciBWB_07_CS6.indd 62 © 2013 Marshall Cavendish International (Singapore) Private Limited 11/10/16 6:15 PM 4. The diagram shows the external view of a person’s heart. X (a) If there is a blockage at X, state what will happen to this person. The cardiac muscles will be deprived of oxygen and nutrients. A prolonged lack of oxygen causes the heart muscles to die eventually. (b) Suggest two causes of such a blockage. Any two of the following: Diet rich in cholesterol / smoking / stress (c) State two ways in which a person can prevent a blockage at X. Any two of the following: More exercise / No smoking / Proper diet such as avoiding a high-fat diet © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_07.indd 63 Worksheet 7 63 8/25/12 5:01 PM Section C: Free-Response Questions Answer the following questions. 1. Arteries and veins are two different types of blood vessels found in the human body. Compare and contrast the structures of arteries and veins with reference to their functions. Arteries (except the pulmonary artery) carry blood away from the heart, while veins (except the pulmonary vein) carry blood back to the heart. Arteries have thick, muscular walls to withstand the high pressure of the blood being pumped out of the heart. The walls are also elastic, so that they can stretch and recoil, helping to push the blood along the artery in spurts. The blood pressure in the veins is much lower than in the arteries, and blood flows more slowly and smoothly in the veins. Hence, the walls of veins are not as thick and muscular as those of arteries of about the same size. Veins also contain less elastic tissue. Most veins have internal valves along their length to prevent backflow of blood. Arteries do not contain internal valves. 2. Describe the route taken by the blood from the intestine to the kidney. List all the major blood vessels and organs involved. Blood flows from the intestine via the hepatic portal vein to the liver, then via the hepatic vein and the posterior vena cava to the heart, then via the pulmonary artery to the lungs, then via the pulmonary vein back to the heart, and finally, via the aorta and the renal artery to the kidney. 64 Worksheet 7 (S)SciBWB_07_CS6.indd 64 © 2013 Marshall Cavendish International (Singapore) Private Limited 11/10/16 6:15 PM WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives (a) State the components of blood and their roles in transport and defence (b) Relate the structures of arteries, veins and capillaries to their functions MultipleChoice Structured 1, 3, 7 1 4, 6 (c) Identify the main blood vessels to and from the heart, lungs, liver and kidneys Textbook section(s) 8.2, 8.3 1 8.4 1, 2 8.5 (d) Describe the structure and function of the heart in terms of muscular contraction and the working of valves 5 2, 3 8.5 (e) Outline the cardiac cycle in terms of what happens during systole and diastole 2 2, 3 8.5 4 8.6 (f) Describe coronary heart disease in terms of the occlusion of coronary arteries, and list the possible causes, such as diet, stress and smoking, stating the possible preventive measures © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_07_CS6.indd 65 FreeResponse Worksheet 7 65 11/10/16 6:16 PM (S)SciBioWB_07.indd 66 8/25/12 5:01 PM Class: Worksheet 8 ( Name: ) Date: Transport in Plants Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. for Chapter 9 CONNECT IT! Transport in plants things transported Water and minerals Food Glucose produced by photosynthesis is converted into sucrose and amino acids enter Root hair cells by Differences in water potential between solution in soil and cell sap due to Osmosis Diffusion transported to other plant parts by Active transport Translocation minerals and ions water transported to other plant parts by Loss of water vapour from causes upward movement in via Xylem make up Phloem Vascular Transpiration the aerial parts of a plant in excess will result in tissues Temperature rate of transpiration affected by Wind speed Wilting Humidity Light intensity © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_08.indd 67 Worksheet 8 67 8/25/12 5:04 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. The diagram shows sections of a leaf and of the stem of a plant. 1 3 2 4 Leaf Stem Which regions represent the tissues that transport manufactured food substances? 2. Leaf Stem A 1 3 B 1 4 C 2 3 D 2 4 ( C ) The diagram shows a section from the stem of a plant. P Q What substances are present in the parts labelled P and Q? 68 Substance in P Substance in Q A Amino acids Sucrose B Sucrose Amino acids C Sucrose Water D Water Water Worksheet 8 (S)SciBioWB_08.indd 68 ( C ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:04 PM 3. The diagram shows a section from the leaf of a plant. X The structure labelled X A B C D 4. . absorbs green light is capable of carrying out photosynthesis prevents sugar loss prevents excessive water loss ( D ) Part of the branch in the diagram below has been ‘ringed’. What will happen to apple Y after some time? apple X apple Y A B C D Grows bigger Remains the same Same size as apple X at the end of the experiment Shrinks in size © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_08.indd 69 ( D ) Worksheet 8 69 8/25/12 5:04 PM 5. Which conditions would cause a plant to wilt most rapidly? A B C D 6. High humidity, high temperature, high wind speed High humidity, low temperature, high wind speed Low humidity, high temperature, high wind speed Low humidity, low temperature, high wind speed ( C ) Which graph shows the effect of air movement (wind speed) on the transpiration rate of a plant? A B Rate of transpiration Rate of transpiration Wind speed C Wind speed D Rate of transpiration Rate of transpiration Wind speed 7. Translocation in plants is defined as A B C D 70 ( C ) . the loss of air from the stomata the loss of water vapour from the stomata the transport of manufactured food in the phloem tissue the transport of water in the phloem tissue Worksheet 8 (S)SciBWB_08_CS6.indd 70 Wind speed ( C ) © 2013 Marshall Cavendish International (Singapore) Private Limited 11/10/16 6:16 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. A group of students tested leaves from two different plants, thyme and mint, in order to find out which leaf surface had more stomata. The thyme plant had fewer leaves compared to the mint plant. Average weight loss/% Mint Thyme Petroleum jelly on both leaf surfaces 14 13 Petroleum jelly on lower leaf surface only 41 20 Petroleum jelly on upper leaf surface only 44 30 (a) From which surface did the mint leaf lose the most water? Explain your answer. The lower leaf surface, because when the jelly was on the upper leaf surface, the weight loss was the highest. (b) Which surface of the thyme leaf has more stomata? Explain your answer. Lower leaf surface, because the weight loss was the highest only when the jelly was placed on the upper leaf surface. When there are more stomata, more water is lost. (c) Explain the difference in the results for the two types of leaves. Thyme loses less water than mint plant. This could be because thyme has fewer leaves, that is, a smaller surface area, or because it has fewer stomata. (d) Based on the experimental results, which plant would be better adapted to hotter, drier conditions? Give a reason for your answer. Thyme, because the percentage of water loss is lower compared with mint. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_08_CS6.indd 71 Worksheet 8 71 11/10/16 6:17 PM 2. The following graphs show the average size of the stomata on the leaves of two plants during a 24-hour period. One plant was kept in a moist environment, while the other was kept in a hot and dry environment. A B Average stomatal size Average stomatal size night day night night day Time/h (a) (i) night Time/h Which graph best represents the plant in a hot and dry environment? B (ii) Explain the reason for your answer in (a)(i). The average stomata size decreases at mid-day, which is the hottest time of the day. With a decreased stomata size, less water will be lost through the stomata. (iii) Explain why the average stomatal size at night is smaller than that during the day. At night, there is no light, hence, photosynthesis does not occur. The sugars accumulated in the guard cells during the day, are used up. This increases the water potential in the guard cells, and water leaves via osmosis. The guard cells become flaccid, and the stomata will close. (b) Predict two structural features that the plant in the hot and dry environment will have that allows it to survive or adapt well in hot and dry conditions. Any two of the following: Reduced surface area of leaves / Sunken stomata / Presence of trichome or hairs in leaf / Thicker cuticle 72 Worksheet 8 (S)SciBioWB_08.indd 72 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:04 PM 3. The following diagrams show the transverse sections of the leaves of two different plants. A B (a) Which plant, A or B, is from a dry environment? Plant A (b) Name and describe two observable features of the plant in (a) which help it to adapt to the dry environment. Any two of the following: Rolled up leaf to make the air around the stomata saturated with water vapour / reduce the surface area exposed to the dry air / Sunken stomata to make the air around the stomata saturated with water vapour / Trichomes (hair) to trap water vapour / Thick cuticle (impermeable/waterproof) to prevent excessive water loss by evaporation 4. The diagram below shows a potometer. shoot rubber tubing reservoir of water scale tap air bubble capillary tube (a) Name the process by which water is lost from the shoot. Transpiration (b) What does the potometer directly measure? It measures the amount of water absorbed by the shoot. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_08.indd 73 Worksheet 8 73 8/25/12 5:04 PM (c) Suggest two reasons why the measurement in (b) is not necessarily the same as the rate of the process stated in (a). Any two of the following: Water taken up by the shoot will be used in photosynthesis. / Water taken up may be used in reactions such as hydrolysis. / Water taken up may be used to maintain plant turgidity. (d) State two factors that might affect the shoot’s rate of water loss. Any two of the following: Temperature / Wind speed or velocity / Humidity / Light intensity LEARN & APPLY Go through the worked example below, then try to answer the Free-Response Questions. Worked Example Explain how a plant absorbs water. Thought Process This question needs to be answered in several parts. The answer should consist of the following points: • Which parts of plants are involved in water absorption? • What processes are involved? • What environmental conditions affect the absorption of water? Answer Points to cover 1. Water absorption occurs mainly at the root hair cells, as they provide a large surface area for absorption. 2. The cell sap of root hairs has lower water potential than the soil solution. 3. Water potential in soil solution is higher than in the root hair cell sap. Water moves from the soil, across the permeable cell wall of the root hair cell, across the partially permeable cell membrane of the root hair cell, into the cell by osmosis. 4. The water potential of the root hair cell is now higher than that of the neighbouring cells. 5. Water thus leaves the root hair cells, and enters the neighbouring cells by osmosis. 6. When the water potential of the soil solution is low, for example, during the dry season, the root hair cells use active transport to absorb dissolved mineral salts. As the mineral salts are dissolved in water, their movement into the plant cells will lower the water potential, and bring water into the cells by osmosis. 74 Worksheet 8 (S)SciBioWB_08.indd 74 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:04 PM Section C: Free-Response Questions Answer the following questions. 1. The diagram below shows a xylem vessel. (a) Are xylem vessels living or dead structures? Give one reason for your answer. They are dead structures. They do not contain cytoplasm. (b) What are the main functions of xylem vessels? State three ways in which xylem vessels are adapted to their functions. They transport water and dissolved mineral salts from the roots to the stem and leaves, and provide mechanical support to the plant. A xylem vessel does not have any cross walls and cytoplasm. This enables water to move easily through the lumen. Lignin deposited on the walls, helps strengthen the walls and prevent the vessel from collapsing. When bundled together, the xylem vessels provide mechanical support to the plant. 2. (a) Describe how the adaptation of a plant root is similar to the adaptation of the lining of the small intestine in humans for the absorption of nutrients. Both are involved in absorption. Since efficient absorption requires high surface area to volume ratio, the root hair is long and narrow, while the lining of the intestine has numerous folds. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_08.indd 75 Worksheet 8 75 8/25/12 5:04 PM (b) Systemic pesticides are pesticides that are absorbed and transported to all parts of the plant. Describe how spraying systemic pesticides directly on the leaves, kills insects such as aphids, which feed on shoots that are grown after the spraying. Systemic pesticides sprayed directly on the plant, are absorbed by the leaves of the plant. These pesticides are then transported to all parts of the plant through the phloem, and accumulated in plant tissues. Insects which feed on shoots that have grown after the spraying period, will take in these pesticides and be killed. 3. Products of photosynthesis, such as sucrose, are translocated via the phloem from sites of production in green leaves to places where they are utilised, such as seeds, fruits, roots and new leaves. These places are known as ‘sinks’. (a) Describe a pathway by which a sucrose molecule is transported from the leaf to a sink such as a fruit. One such pathway by which sucrose is transported, is the route taken by the sucrose molecule from the cells in the leaf to the fruit. The sucrose molecule moves from the mesophyll cells in the leaf to the phloem in a vascular bundle of the leaf. This is followed by movement to the phloem in a vascular bundle of the fruit, and finally to the cells of the fruit. (b) Suggest and provide one reason why a sucrose molecule may be transported to a particular sink and not to other sinks. Sucrose will first be transported to sinks which have higher rates of metabolic activity, such as growing points (shoot and root tips). In these locations, sucrose can be converted into glucose. The glucose produced can then be used for tissue respiration to release energy for metabolic activity. 76 Worksheet 8 (S)SciBioWB_08.indd 76 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:04 PM 4. A student set up a potometer to investigate the amount of water taken up by a leafy shoot. shoot rubber tubing reservoir of water scale tap air bubble capillary tube The distance that the air bubble travelled along the capillary tube, was measured every two minutes. The results of the experiment are shown in the table below. Time/minutes 0 2 4 6 8 10 12 14 16 18 20 Distance travelled/mm 0 7 20 33 45 57 72 85 99 108 122 22 24 (a) Draw a graph to show the results. Distance travelled/mm 125 Graph of distance travelled against time taken × × 100 × × 75 × × 50 × × 25 × × 0 × 2 4 6 8 10 12 14 16 18 20 Time/min © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_08.indd 77 Worksheet 8 77 8/25/12 5:04 PM (b) Comment on the graph with reference to water uptake and water loss in the plant. The movement of the air bubble along the capillary tube indicates the uptake of water by the plant. The distance travelled is proportional to the volume of water taken up by the plant. From the graph, the rate of water uptake by the plant is proportional to the rate of water loss through transpiration. The rate of water uptake by the plant can be determined by calculating the gradient of the line, 102.5 – 5 which is 17.2 – 0.8 mm/min = 5.95 mm/min. (c) Describe how water from the capillary tube enters the shoot to reach one of the leaves. Transpiration in the leaves creates a transpiration pull, which draws water up the xylem vessels to the leaves; water in the container enters the shoot to replace the water lost in transpiration. WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives (a) Identify the positions, and explain the functions of xylem vessels and phloem in sections of a herbaceous dicotyledonous leaf and stem under the light microscope MultipleChoice Structured 1, 2 4, 7 (d) Relate the structure and function of root hairs to their surface area, and to water and ion update (e) State what is transpiration (f) Describe the effects of variation of air movement, temperature, humidity and light intensity on transpiration rate, and explain how wilting occurs 78 Worksheet 8 (S)SciBioWB_08.indd 78 Textbook section(s) 9.1 (b) Briefly explain the movement of water through the stem in terms of transpiration pull (c) Define translocation FreeResponse 1 9.2 3 9.2 2 9.3 3 1, 4 4 9.4 5, 6 2, 3, 4 4 9.4 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:04 PM Class: Worksheet 9 ( Name: ) Date: Respiration in Humans for Chapter 10 CONNECT IT! Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. Oxidation of food substances e.g. glucose, with the release of energy Respiration types oxygen oxygen not required produces small amount of energy Anaerobic Aerobic equation in human cells equation in human muscle cells Glucose Lactic Glucose + Oxygen acid required in humans, oxygen obtained and carbon dioxide removed (gas exchange) by produces large amount of energy Carbon + dioxide Water Breathing chemical contents of cigarette smoke Smoking Nicotine Tar Carbon monoxide Irritants Inspiration damages Expiration air rushes in Bronchioles Bronchi Larynx Trachea Nasal passages air rushes out lined with cilia Alveoli gas dissolves in moist lining supplied with oxygen diffuses out into Capillaries + O2 Red contain blood cells Oxyhaemoglobin Haemoglobin – O2 red blood cells carry oxygen to cells carbon dioxide Hydrogencarbonate ions (in blood plasma) Carbonic acid © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_09.indd 79 Carbon dioxide + Water oxygen used for respiration Worksheet 9 79 9/6/12 10:11 AM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. Cellular respiration is best described as A B C D 2. a process by which carbon dioxide is inhaled, and oxygen is exhaled a process by which oxygen is inhaled, and carbon dioxide is exhaled a process by which sugar is produced from carbon dioxide and water in the presence of light and chloroplasts a process by which sugars are oxidised to release chemical energy ( D ) . The site of aerobic respiration is the A B C D 3. . chloroplast endoplasmic reticulum mitochondrion nucleus ( C ) The diagram below shows part of the human respiratory system. Identify the trachea. I II III A B C D 80 I II III IV Worksheet 9 (S)SciBioWB_09.indd 80 IV ( B ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:05 PM For questions 4 and 5, refer to the experimental set-up shown below. original position of drop of coloured water air in syringe X Y capillary tube grasshopper wire gauze sodium hydroxide pellets 4. What is the role of the sodium hydroxide pellets? A B C D 5. ( A ) After a while, what would be the position of the drop of coloured water? A B C D 6. To absorb carbon dioxide To absorb oxygen To absorb water vapour To act as food for the grasshopper Disappears from the capillary tube Nearer position X Nearer position Y No change in position Which of the following correctly describes the diagram below, which depicts the exchange of gases in the human circulatory system? P 100 mm Hg O2 40 mm Hg CO2 40 mm Hg O2 46 mm Hg CO2 A B C D R is a blood capillary, and blood flow is from P to Q R is a blood capillary, and blood flow is from Q to P R is an alveolus, and blood flow is from P to Q R is an alveolus, and blood flow is from Q to P © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_09.indd 81 ( B ) R Q 95 mm Hg O2 40 mm Hg CO2 ( C ) Worksheet 9 81 8/25/12 5:05 PM 7. A person's breathing rate over a period of time is shown below. Breathing rate/ arbitrary units 0 1 2 3 4 5 6 7 8 9 10 Time/minutes Which statement best describes the change in the person's breathing rate from the 1-minute point to the 3-minute point? A B C D The person is dying. The person is exercising. The person is listening to music. The person is sleeping. ( B ) Section B: Structured Questions Answer the following questions in the spaces provided. 1. The following experiment was performed to demonstrate that microorganisms in the soil respire. B A soil concentrated potassium hydroxide solution C D hydrogencarbonate indicator (a) What is the error in the experimental set-up? How should this error be corrected? The delivery tube A should be submerged in the concentrated potassium hydroxide solution, so that air will be directed into the concentrated potassium hydroxide solution, before entering test tube C. 82 Worksheet 9 (S)SciBioWB_09.indd 82 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:05 PM (b) Assuming that the error has been corrected, how should the pump draw air through the apparatus? Should it be from A to B or from B to A? From A to B (c) In which test tube, C or D, would the hydrogencarbonate indicator solution change in appearance? D (d) What causes the change in appearance of the hydrogencarbonate indicator solution? Microorganisms in the soil respired and released carbon dioxide, which dissolved in the hydrogencarbonate indicator solution to form carbonic acid. (e) Write a word equation to illustrate aerobic respiration in soil microorganisms. Glucose + Oxygen 2. Carbon dioxide + Water + Energy The graph below shows changes in the concentration of lactic acid in the blood of a person performing vigorous exercise. Concentration of lactic acid X Y Z Time (a) In which region, X, Y or Z, is the person actually exercising? Y (b) Write a word equation to show how lactic acid is formed during vigorous exercise. Glucose Lactic acid + Energy © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_09.indd 83 Worksheet 9 83 8/25/12 5:05 PM (c) Describe the effects of an increasing lactic acid concentration on the muscles during Y. The increasing amount of lactic acid will cause the person to suffer from muscle pains and fatigue. (d) Explain why there is a gradual decrease in lactic acid concentration in region Z. The person stops exercising and rests. The lactic acid is removed from the muscles, and transported to the liver, where it is oxidised to release energy. 3. The figure below shows the human respiratory system. A B rib C D E F Identify structures A to F. A: Larynx B: Trachea C: Bronchus D: Bronchiole E: Alveolus/Alveoli F: Diaphragm 84 Worksheet 9 (S)SciBioWB_09.indd 84 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:05 PM 4. The diagram below shows a section of the alveolus. Gas in Gas out Direction of blood flow Using the diagram provided, state two adaptations of an alveolus which allow it to exchange gases effectively in the lungs. Any two of the following: It is highly folded and spherical in shape, hence, it has a large surface area. / Wall is one-cell thick, so it is easy for gases to diffuse through. / Its surface is lined with moisture for oxygen to dissolve in. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_09_CS6.indd 85 Worksheet 9 85 11/10/16 6:18 PM LEARN & APPLY Go through the worked example below, then try to answer the Free-Response Questions. Worked Example Describe the movement of carbon dioxide from the body cells to the air spaces in the lungs. Thought Process You need to state how carbon dioxide is produced in body cells, as well as, how carbon dioxide is transported from the cells to the lungs. Think about the process and reactions involved. Answer Points to cover 1. Carbon dioxide, which is produced in cells during cellular respiration, diffuses out into the tissue fluid before diffusing into the capillary network. 2. This carbon dioxide will diffuse into blood in the capillaries due to a concentration gradient of carbon dioxide between the cells and the blood. 3. Carbon dioxide diffuses into red blood cells and is converted into hydrogencarbonate ions. 4. Hydrocarbonate ions then diffuse into blood plasma and are carried to the lungs. 5. In the lungs, the hydrogencarbonate ions diffuse into red blood cells and are converted into carbon dioxide. 6. Carbon dioxide then diffuses across the blood capillaries into the air spaces in the alveoli of the lungs, due to the concentration gradient of carbon dioxide between the red blood cells and the air spaces in the lungs. Section C: Free-Response Questions Answer the following questions. 1. Describe three ways in which smoking damages the lungs. Tar deposited in the lungs contains carcinogens and increases the risk of lung cancer. Carbon monoxide in smoke increases fat deposition on the inner surface of arteries, which may result in blockage of the coronary arteries. This might cause a heart attack, as less oxygen is transported to the heart. Irritants cause coughing. Prolonged violent coughing could lead to emphysema, in which the alveolar walls break down. 86 Worksheet 9 (S)SciBWB_09_CS6.indd 86 © 2013 Marshall Cavendish International (Singapore) Private Limited 11/10/16 6:18 PM 2. The diagram below shows a section of an alveolus and a blood capillary near it. Annotate the diagram to describe the movement of oxygen when it enters the lungs. high oxygen concentration in air from atmosphere red blood cells leave the lungs; oxygen bound to haemoglobin is carried to the heart oxygen dissolves in layer of moisture lining alveolus low oxygen concentration in red blood cells entering the lungs dissolved oxygen diffuses through one-cell thick capillary wall into red blood cells, and combines with haemoglobin © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_09_CS6.indd 87 Worksheet 9 87 11/10/16 6:18 PM WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives MultipleChoice Structured (a) Identify parts of the human respiratory system in diagrams 3 3 (b) State the characteristics of the exchange surface of alveoli, and describe the role it plays in gas exchange 6 4 (c) Describe the effects of tobacco smoke on our health (d) Define and state the equation (in words only) for aerobic respiration in humans 88 Textbook section(s) 10.3 2 10.3 1 10.5 1 10.1 (e) Define and state the equation (in words only) for anaerobic respiration in humans 2 10.1 (f) Describe the effect of lactic acid in muscles during exercise 2 10.1 Worksheet 9 (S)SciBioWB_09.indd 88 1, 2, 4, 5, 7 FreeResponse © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:05 PM Class: Worksheet 10 ( Name: ) Date: Coordination and Response for Chapters 11, 12 and 13 CONNECT IT! Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. PNS: Peripheral nervous system Receptors Nerves Coordination and response Nervous Control Hormonal Control carried out by Hormones produced by CNS: nervous system Central Endocrine glands e.g. Spinal cord Brain Adrenal glands Islets of Langerhans (in pancreas ) produce produce consists of Sensory Stimulus neurone received by travels along Sense organ Relay / Intermediate to neurone to Motor Impulse produces neurone e.g. Eye Insulin Adrenaline Effector to Reflex action produces e.g. pupil reflex Sensory neurone in optic nerve Bright light Retina receives to via relay neurone Brain Motor neurone produces Light Cornea refracted by Lens onto Retina focal length controlled by sends impulses through Optic nerve to Brain Suspensory ligament Iris muscles Pupil constricts, reducing amount of light entering eye Impulse and to to travels along received by Ciliary muscles © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_10.indd 89 Glucagon interprets image Worksheet 10 89 8/25/12 5:06 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. Which of the following correctly identifies X and Y? X 2. Y X Y A Motor neurone Motor neurone B Motor neurone Sensory neurone C Motor neurone Relay neurone D Sensory neurone Motor neurone ( D ) A nerve impulse is transmitted along three neurones as shown below. W Y X Z The sensory neurone is represented by A B C D 90 . W X Y Z ( B ) Worksheet 10 © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_10.indd 90 8/25/12 5:06 PM 3. Cataract is a medical condition in which patients experience blurred/cloudy vision due to difficulties in focusing light rays onto the retina. Which part of the eye is affected in this condition? P Q S R A B C D 4. 5. P Q R S ( C ) Which of the following correctly describes the changes that take place when a person looks at a distant object? Ciliary muscle Suspensory ligament Shape of lens A Contracts Becomes less taut Becomes more convex B Contracts Becomes taut Becomes less convex C Relaxes Becomes less taut Becomes less convex D Relaxes Becomes taut Becomes less convex ( D ) The diagram shows the front view of the pupil of a person in a bright room. radial muscle circular muscle What happens when the light in the room is switched off? Radial muscle Circular muscle Pupil size A Contracts Relaxes Decreases B Contracts Relaxes Increases C Relaxes Contracts Decreases D Relaxes Contracts Increases © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_10.indd 91 ( B ) Worksheet 10 91 8/25/12 5:06 PM 6. Hormones are destroyed in the A B C D 7. . kidney liver pancreas stomach ( B ) . The function of the hormone insulin is to A B C D allow glucose to enter the bloodstream break down glucose prevent glucose from being excreted by the kidney trigger the conversion of glucose into glycogen ( D ) For questions 8 and 9, refer to the diagram shown below. Hormone X secreted Blood glucose concentration increases Blood glucose concentration decreases Hormone Y secreted 8. 9. Which gland will secrete hormone X, and when will it be secreted? Gland secreting hormone X When A Islets of Langerhans After a meal B Islets of Langerhans During exercise C Liver After a meal D Liver Before a meal ( A ) What is the identity of hormones X and Y? Hormone X Hormone Y A Adrenaline Insulin B Glucagon Glycogen C Glycogen Adrenaline D Insulin Glucagon ( D ) 92 Worksheet 10 (S)SciBioWB_10.indd 92 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:06 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. The diagram below shows a specialised cell. Y X (a) Name the type of neurone shown. It is a sensory neurone. (b) Part X is connected to a receptor. Give one example of such a receptor. The nerve endings in the skin/eye (or any sense organ) (c) What does part Y connect to? The central nervous system / dendrites of a relay neurone (d) What is the role of this specialised cell? To conduct nerve impulses from the sensory receptor to the central nervous system 2. A person moved from a brightly-lit room to a dark room. He was unable to see for the first few minutes. Gradually, he was able to see fairly well in the dark room. (a) Explain how the pupils of eyes in a brightly-lit room differ from those in a dark room. The pupils of eyes in a brightly-lit room have a smaller diameter (constrict) due to the contraction of the circular muscle and relaxation of the radial muscle in the iris. In a dark room, the pupils of eyes will have a larger diameter (dilate) to allow more light to pass into the eyes. The radial muscles contract and the circular muscles relax. (b) Explain why the person was not able to see when he first stepped into the dark room. Visual purple is bleached in bright light. In the dark, it takes some time for visual purple to be re-formed in the rods; only then will the rods be sensitive to dim light. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_10.indd 93 Worksheet 10 93 8/25/12 5:06 PM 3. (a) What are endocrine glands? Endocrine glands are ductless glands that secrete hormones directly into the bloodstream. (b) Give an example of an endocrine gland. Adrenal gland / Islets of Langerhans / Gonads / Pituitary gland / Thyroid gland (c) Describe a hormone with reference to the endocrine gland that produces it, and state its effect(s) on its target organ(s). Insulin is produced by the islets of Langerhans (pancreas), and is secreted into the bloodstream. Insulin is then transported to the liver and muscles (target organs), where it decreases the blood glucose concentration by stimulating cells to increase their uptake of glucose, and to convert glucose into glycogen for storage. (d) Besides hormones, how else can information be relayed in the body? Information can be relayed via nerve impulses. 4. When the blood glucose level in the body decreases, the hormone glucagon is released to restore the blood glucose level. (a) Suggest a scenario where the blood glucose level decreases below the normal level. An example would be during exercise / starvation. (b) Explain how glucagon helps to restore the blood glucose level. Glucagon stimulates liver and muscle cells to convert glycogen into glucose, which is then released into the bloodstream. (c) Where is glucagon secreted from? It is secreted from the islets of Langerhans in the pancreas. 94 Worksheet 10 (S)SciBioWB_10.indd 94 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:06 PM LEARN & APPLY Go through the worked example below, then try to answer the Free-Response Questions. Worked Example Compare and contrast nervous control and hormonal control. Thought Process In the answer, both similarities and differences between nervous control and hormonal control are required. Think about the functions of these controls, their effects and their speed of action. Answer Points to cover 1. Both nervous and hormonal controls are used to control processes in the body to maintain a constant internal environment (homeostasis). 2. Both require a stimulus to cause the transmission of a message to target organ. 3. Nervous control involves nervous impulses (electric signals), while hormonal control involves hormones (chemical substances). 4. The response in nervous control is quick, while that in hormonal control is relatively slower. 5. The response in nervous control is short-lived, while the response in hormonal control may either be short-lived or long-lived. 6. Nervous control may be voluntary or involuntary, while hormonal control is always involuntary. 7. The effect of nervous control is usually localised, while in hormonal control, more than one target organ may be affected. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_10.indd 95 Worksheet 10 95 8/25/12 5:06 PM Section C: Free-Response Questions Answer the following questions. 1. Describe the general structure of the nervous system using the following terms: central nervous system, effector, motor neurone, peripheral nervous system, and sensory neurone. The nervous system is made up of the central nervous system (CNS) consisting of the brain and the spinal cord, the peripheral nervous system (PNS) consisting of the cranial nerves from the brain, the spinal nerves from the spinal cord, and the sense organs. Since the sense organs receive stimuli, they are known as receptors. In response to stimuli, sensory neurones (receptor neurones) will transmit impulses from the receptor to the CNS. A motor neurone (effector neurone) then transmits impulses from the CNS to the effector such as muscle fibres, which will carry out the required action. 2. Explain the nervous pathway involved in the following scenarios. (a) The pupils of a person’s eyes change when the person moves from a dark room to a bright room. The size of the pupil determines the amount of light entering the eye. The size of each pupil is controlled by two sets of involuntary muscles in the iris, namely, the circular muscles and the radial muscles. When a person tries to see in a bright room after moving from a dark room, impulses are produced that send signals to the central nervous system (CNS). The CNS, in turn, generates signals via motor neurones, which cause the circular muscles of the iris to contract, and the radial muscles to relax. This allows each pupil to constrict, therefore decreasing the amount of light entering the eyes. 96 Worksheet 10 (S)SciBioWB_10.indd 96 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:06 PM (b) The lenses of a person's eyes change shape when the person focuses on a distant object. When focusing on a distant object, the ciliary muscles relax, pulling the suspensory ligaments. The suspensory ligaments then become taut, and in turn pull the edge of the lens. This causes the lens to become thinner and less convex, which increases its focal length. Light rays from the distant object will then be sharply focused on the retina, causing photoreceptors to be stimulated. Nerve impulses produced are then transmitted by optic nerves to the central nervous system (CNS). The brain then interprets the impulses, causing the person to see the distant object. WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives MultipleChoice Structured FreeResponse (a) Understand how components of the nervous system, such as the brain, spinal cord and nerves, co-ordinate and regulate bodily functions 2 1 1, 2 (b) Identify sensory neurones, relay neurones and motor neurones; and outline their functions 1 1 3, 4, 5 2 (c) State how the main components of the eye work to produce a focused image of near and distant objects, and how they respond when exposed to different light intensities (d) State what is meant by an endocrine gland 11.2, 11.3 11.2 2 12.1, 12.2 3 13.1, 13.2 (e) Define hormones (with reference to certain examples), identify their mode of action; and state the organ which they are destroyed in 6, 7 3 13.1 (f) Outline how insulin and glucagon regulate blood glucose levels 8, 9 4 13.3 © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_10_CS6.indd 97 Textbook section(s) Worksheet 10 97 11/10/16 6:21 PM (S)SciBioWB_10.indd 98 8/25/12 5:06 PM Class: Worksheet 11 ( Name: ) Date: Reproduction in Plants for Chapter 14 CONNECT IT! Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. Reproduction in plants Offspring genetically identical to parents parts Sexual Asexual Flowering plants in male and female gametes brought together by Offspring genetically different from parents Self - pollination Pollination Sepal may be Cross - pollination Petal land on stigma of female flower of same species Receptacle pollen grains are transferred by Pedicel Anther Stamen parts Filament Carpel Stigma parts Pollen grains Insect Wind after pollination, germinates to form e.g. e.g. Clitoria Ischaemum produces Style Pollen tube Ovary contains male two nuclei travel down Ovule contains fuse with Ovum © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_11.indd 99 Worksheet 11 99 8/25/12 5:10 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. One of the advantages of asexual reproduction is that A B C D . all the beneficial characteristics of the parent are passed on to the offspring less offspring are produced more offspring are produced there is more genetic variation in the offspring ( A ) For questions 2 and 3, refer to the diagram below, which shows the general section of a flower. A D C B 2. 3. Where can male and female gametes be found? Male gamete Female gamete A A B B A C C B A D B D What is structure C? A B C D 4. Ovary Petal Sepal Stigma The carpel consists of A B C D 100 ( D ) anthers and filaments anthers, stigma and style petals, sepals and stamens stigma, style and ovary Worksheet 11 (S)SciBioWB_11.indd 100 ( B ) . ( D ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:10 PM 5. Pollination is A B C D 6. the fusion of male gametes with the female gametes in the ovary the fusion of pollen grains with the female gametes in the ovary the transfer of female gametes to the stigma the transfer of pollen grains to the stigma of a flower Self-pollination refers to the A B C D 7. . ( D ) . fusion of pollen grains from a flower with the ovum of a different flower on another plant fusion of pollen grains from a flower with the ovum of the same flower on the same plant transfer of pollen grains from the anthers of a flower to the stigma of a different flower on another plant transfer of pollen grains from the anthers of a flower to the stigma of the same flower on the same plant ( D ) The diagram below shows the structure of a generalised flower. X Y Z Where do pollination and fertilisation take place? Pollination Fertilisation A X Y B X Z C Y X D Y Z © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_11.indd 101 ( B ) Worksheet 11 101 8/25/12 5:10 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. The diagrams below show a complete flower and a section of a flower of the Kalanchoe plant. A B C (a) Identify structures A to C. A: Anther B: Stigma C: Ovule (b) From what you can see in the diagrams, what feature of the flower suggests that the fruit of this flower is likely to contain more than one seed? There are many ovules in the ovary. 102 Worksheet 11 (S)SciBioWB_11.indd 102 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:10 PM 2. The diagram below shows a section of a flower. G A F B E Pollen Stigma C D (a) Identify structures A to G. A: Ovary E: Anther B: Ovule F: Filament C: Style G: Sepal D: Petal (b) Which of the structures A to G is directly involved in pollination? E (c) After fertilisation, state which structure will develop into (i) a fruit; and A (ii) a seed. B © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_11.indd 103 Worksheet 11 103 8/25/12 5:10 PM Section C: Free-Response Questions Answer the following questions. 1. (a) Distinguish between asexual reproduction and sexual reproduction. Asexual reproduction produces offspring that are identical to the parent, but sexual reproduction produces offspring that are non-identical to the parents. Asexual reproduction involves one parent, but sexual reproduction involves two parents. Sexual reproduction involves fusion of gametes, but asexual reproduction does not involve fusion of gametes. Asexual reproduction involves mitosis, but sexual reproduction involves both meiosis and mitosis. (b) Discuss the advantages and disadvantages of asexual reproduction. Advantages: -– The offspring produced are identical to the parent, so there is a higher chance of the good characteristics of the parent being passed on to the offspring. -– Only one parent is involved. It does not depend on external agents like wind. Disadvantage: -– There will be no genetic variation among the offspring. Therefore, the offspring will be less adapted to changes in the environment. (c) What are some special features of plants that enable them to carry out sexual reproduction? Plants may have flowers. These are reproductive organs that enable plants to carry out sexual reproduction. The parts of the flower that are involved in the reproduction process are the stamen and pistil. The stamen consists of the anther and filament, with the anther producing male gametes. The pistil consists of the stigma, style and ovary. The ovary contains at least one ovule with a female gamete. 104 Worksheet 11 (S)SciBWB_11_CS6.indd 104 © 2013 Marshall Cavendish International (Singapore) Private Limited 11/10/16 6:21 PM 2. (a) Distinguish between pollination and fertilisation in flowering plants. Pollination is the transfer of pollen grains from the anther to the stigma. Fertilisation is the fusion of the male gamete with the female gamete to produce a zygote. (b) Outline the growth of the pollen tube and its entry into the ovule. Step 1: After pollination, the pollen grains germinate in response to the sugary fluid secreted by the mature stigma. Step 2: A pollen tube grows out from each pollen grain. Step 3: The cytoplasm of each pollen grain enters the pollen tube. Step 4: As the pollen tube grows, it secretes enzymes to digest the surrounding tissue of the stigma and style. Thus, the pollen tube penetrates the style as it grows. Step 5: The pollen tube enters the ovule usually through an opening in the ovule wall called the micropyle. Step 6: Within the ovule, the tip of the pollen tube absorbs sap and bursts, releasing two male gametes. (c) Describe fertilisation in flowering plants. Fertilisation in flowering plants occurs in the following steps: Step 1: Pollen settles on a mature stigma. The stigma secretes a sugary fluid. Step 2: Pollen grain produces a pollen tube. Step 3: Pollen tube grows downwards towards the ovule, enters the ovule, and releases two male gametes. Step 4: One male gamete fuses with the ovum to form a zygote. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_11.indd 105 Worksheet 11 105 8/25/12 5:10 PM WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives (a) Define sexual and asexual reproduction, and differentiate between the two processes (b) Identify the main parts of a flower in diagrams/photos MultipleChoice 1 2, 3, 4 (c) State the functions of the sepals, petals, anthers and carpels (d) Outline the process of pollination, and distinguish between self-pollination and cross-pollination Structured 5, 6, 7 FreeResponse 1 106 Worksheet 11 (S)SciBioWB_11.indd 106 14.1 1(a), 2(a) 14.2 2(b) 14.2 2(b) 2 (e) State the characteristics of insect-pollinated and wind-pollinated flowers, and distinguish between the two types of flowers (f) Describe the growth of the pollen tube and the process of fertilisation in flowering plants Textbook section(s) 14.3 14.3 1(b), 2(c) 2 14.4 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:10 PM Class: ) Worksheet 12 ( Name: Date: Reproduction in Humans Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. for Chapter 15 CONNECT IT! Seminal vesicles Reproduction in humans Cowper’s gland secrete fluid that mixes with sperms to form Prostate gland Cervix Semen Scrotum Organ systems Uterus ejaculated into vagina during sexual intercourse Penis Male Female Urethra Oviducts Vagina Sperm Ovaries Epididymis and progesterone produce fuse Ova (singular: ovum) Sperm Testosterone Gestation Fertilisation control may spread some STI-causing agents Testes produce Oestrogen duct Zygote Embryo Fetus Birth day 1–5 Menstruation Menstrual cycle (for a 28-day cycle) Ovulation day 14 © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_12_CS6.indd 107 Worksheet 12 107 11/10/16 6:22 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. Sperms are produced in the A B C D 2. . epididymis penis sperm duct testes ( D ) The diagram below shows a side view of the male reproductive system. X Y Z What is the function of X, Y and Z? X 3. Z A Passage for both urine and sperms Passage for sperms Production of sperms B Passage for both urine and sperms Production of sperms Passage for sperms C Passage for sperms Passage for both urine and sperms Production of sperms D Production of sperms Passage for sperms Passage for both urine and sperms ( C ) Where does fertilisation occur? A B C D 108 Y Cervix Ovary Oviduct Uterus Worksheet 12 (S)SciBioWB_12.indd 108 ( C ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:11 PM 4. The following diagram shows the concentration of female sex hormones in the blood over time. Hormone level Key oestrogen progesterone X Time Y What happens at point X and point Y? 5. X Y A Menstruation Ovulation B Menstruation Repair of uterus wall lining C Repair of uterus wall lining Menstruation D Repair of uterus wall lining Ovulation Risk of Human Immunodeficiency Virus (HIV) infection is best reduced by . A B C D ( C ) not having blood transfusions regular exercise and a healthy diet keeping to one sexual partner taking anti-viral medications © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_12.indd 109 ( D ) Worksheet 12 109 9/4/12 2:38 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. The diagram below shows how the thickness of the uterine lining changes over time during a menstrual cycle lasting 28 days. Thickness of uterine lining 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Days (a) On which day(s) is the woman most fertile? Day 13 to 15 (b) What causes the decrease in the thickness of the uterine lining from day 0 to day 5? Menstruation occurs. The uterine lining breaks down and is discharged from the body due to the drop in progesterone production that occurs after the egg is not fertilised. (c) If the woman is pregnant, what will happen to the thickness of the uterine lining after day 28? The uterine lining will remain thick and spongy with blood vessels. 110 Worksheet 12 (S)SciBioWB_12.indd 110 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:11 PM (d) On the diagram below, draw a graph to show how the level of progesterone changes over the course of the menstrual cycle, assuming that the woman is not pregnant. Progesterone level 1 2. 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Days The diagram below shows the front view of the female reproductive system. A X C B D E (a) Identify structures A to E. A: Oviduct B: Ovary C: Uterine wall / Uterus / Uterine lining D: Cervix E: Vagina (b) State the functions of structures A to C. A is the oviduct, which is the passage for the egg as well as the site of fertilisation. B is the ovary, which is the place where the eggs/ova develop. C is the uterine wall, which is the site for embryo implantation. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_12.indd 111 Worksheet 12 111 8/25/12 5:11 PM (c) How does the shape of structure A at point X help its function? Structure A is a narrow muscular tube leading from B to C. It has a funnel-like opening at X, which makes it easier for the egg to enter. Section C: Free-Response Questions Answer the following questions. 1. Distinguish between the following terms: (a) semen and sperm; and Sperm is the male gamete produced in the testes. Semen is a fluid containing sperms. This fluid is first secreted from the prostate gland and Cowper’s gland, before it is mixed with sperms to activate them. (b) ovary and oviduct. The ovary produces eggs and female sex hormones. When eggs mature, they are released from the ovary. The oviduct is a narrow muscular tube, which connects the ovary to the uterus. The mature eggs released from the ovary, travel along the oviduct into the uterus. Fertilisation occurs in the oviduct. 112 Worksheet 12 (S)SciBWB_12_CS6.indd 112 © 2013 Marshall Cavendish International (Singapore) Private Limited 11/10/16 6:23 PM 2. The table below shows the number of reported HIV/AIDS (Acquired Immune Deficiency Syndrome) cases in Singapore from year 2000 to 2010. Year Total number of HIV/AIDS cases 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 226 237 234 242 311 317 359 423 456 463 441 (Source: Ministry of Health, Singapore 2011) (a) Describe the general trend observed for the number of HIV/AIDS cases in Singapore over the last 10-year period. The number of HIV/AIDS cases in Singapore increased over the last 10 years. (b) Explain how HIV/AIDS can be transmitted. AIDS is caused by the human immunodeficiency virus (HIV). HIV is transmitted through the exchange of body fluids such as semen/blood. As such, HIV/AIDS can be transmitted by having unprotected sex with an infected person. It can also be transmitted by sharing needles and syringes used by an infected person for injecting drugs. (c) Describe ways in which the number of HIV/AIDS cases can be reduced. Not be promiscuous / Have one sexual partner only Use of condoms No sharing of needles/syringes © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_12.indd 113 Worksheet 12 113 8/25/12 5:11 PM WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives Structured FreeResponse 1, 2 2 1(a), 1(b) 15.2, 15.3 (b) Describe stages in the menstrual cycle with reference to the effect of hormones 4 1 1(c) 15.4 (c) Describe fertilisation and the process of early development of the zygote 3 (d) Understand how the human immunodeficiency virus (HIV) can be transmitted between individuals, and suggest methods to control the spread of the disease 5 (a) Identify on diagrams, structures in the male and female reproductive systems, and state their respective functions 114 Textbook section(s) MultipleChoice Worksheet 12 (S)SciBioWB_12.indd 114 15.5 2 15.6 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:11 PM Class: ) Worksheet 13 ( Name: Date: Heredity and Molecular Genetics for Chapters 16 and 17 CONNECT IT! Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. Deoxyribose sugars Phosphate groups consist of Nucleotides made up of Nitrogenous bases (A, T, C, G) and environment Represented by letters e.g. T or t, IO or IA Allele part of Genes sequences DNA of found in that code for characteristics expressed in homozygous(TT) and heterozygous(Tt) conditions Recessive expressed in only homozygous(tt) condition Homozygous Heterozygous tt or TT Tt leads to Variation variation Discontinuous variation Complete dominance e.g. additive effect of many genes Tall or dwarf pea plants can cause Mutation types Continuous in humans may be Autosome e.g. in pea plants Dominant Chromosomes Phenotype affects Genotype may be lead to different forms of the same gene Sex chromosome Mutagen Gene mutation e.g Hb gene mutation causes Sickle-cell anaemia Chromosomal mutation e.g. Trisomy ( three copies) of chromosome 21 caused by a few genes causes Down's syndrome © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_13_CS6.indd 115 Worksheet 13 115 11/10/16 6:23 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. Phenotype is determined by A B C D . environmental factors only genotype and environmental factors genotype only mutation only ( B ) For questions 2 and 3, refer to the following diagram showing the family tree of a family with phenylketonuria (PKU). PKU is a disease that is expressed in homozygous recessive individuals. PKU patients cannot break down the amino acid called phenylalanine. Key normal female normal male P PKU female Generation I PKU male R Q S Generation II Generation III T 2. Which of the following best describes the genotype of individuals in the family tree? A B C D 3. ( B ) What is the chance that T will be normal? A B C D 116 P and Q are homozygous dominant. P and Q are heterozygous. R and S are homozygous dominant. R is homozygous dominant, and S is heterozygous. 25% 50% 75% 100% Worksheet 13 (S)SciBioWB_13.indd 116 ( C ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:12 PM 4. The Rhesus blood group system is a human blood group system. It is the most clinically important blood group system after ABO. In humans, the allele for the Rhesus-positive trait (H) is dominant to the allele for the Rhesus-negative trait (h). If a homozygous Rhesus-positive woman and a homozygous Rhesus-negative man have children, what is the probability of their first child being Rhesus-positive? A B C D 5. 22 22 23 23 chromosomes chromosomes chromosomes chromosomes and and and and one one one one X X X X . chromosome or Y chromosome chromosome or Y chromosome ( A ) . Sickle-cell anaemia is an example of a A B C D 7. ( D ) The nucleus of the human female gamete contains A B C D 6. 0% 25% 75% 100% bacterial infection chromosomal mutation dietary deficiency disease gene mutation ( D ) The graphs below show the distributions, within a human population, for height and for earlobe type. Percentage of population Percentage of population 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Height/m Free earlobe Attached earlobe Which type of variation does each characteristic exhibit? Height Type of earlobe A Continuous Continuous B Continuous Discontinuous C Discontinuous Continuous D Discontinuous Discontinuous © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_13.indd 117 ( B ) Worksheet 13 117 8/25/12 5:12 PM 8. Which of the following characteristics is an example of continuous variation? A B C D 9. Ability to roll tongue Blood type Gender Length of feet ( D ) The following diagram shows part of a DNA molecule. X Y Z Identify X, Y and Z. X Y Z A Deoxyribose sugar Nitrogenous base Phosphate group B Nitrogenous base Deoxyribose sugar Phosphate group C Phosphate group Deoxyribose sugar Nitrogenous base D Phosphate group Nitrogenous base Deoxyribose sugar ( C ) 10. Which of the following correctly lists all the nitrogenous bases in DNA? A B C D 118 Adenine, cytosine, guanine, thymine Adenine, cytosine, guanine, uracil Alanine, cytosine, guanine, thymine Alanine, cytosine, guanine, uracil Worksheet 13 (S)SciBioWB_13.indd 118 ( A ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:12 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. The sex of a person is determined by the combination of X and Y chromosomes present. Female cells have two X chromosomes, while male cells have one X and one Y chromosome. (a) State whether the male or the female gamete determines the sex of a child. Male gamete (b) In the space below, draw a genetic diagram to show that there is an equal chance that a baby can be a boy or a girl. Parental phenotypes Parental genotypes Male XY X Y X X XX Female XX Female XY Male XY Male Gametes Offspring genotypes Offspring phenotypes Female XX ⴛ Offspring phenotypic ratio Female : Male = 1 : 1 (c) The following diagram shows all the chromosomes (the karyotype) of a person. 1 13 2 14 15 3 16 4 17 18 5 19 © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_13.indd 119 6 20 7 8 21 9 10 22 11 12 23 Worksheet 13 119 8/25/12 5:12 PM (i) Identify the sex of the person from which the chromosomes were taken. Explain your answer. The person would be a male, because the 23rd pair of chromosomes consists of an X chromosome and a Y chromosome. (ii) Suggest, with a reason, what condition will be present in this person. The condition will be Down’s syndrome, because an extra chromosome 21 is present. 2. Sickle-cell anaemia is a condition in which red blood cells contain abnormal haemoglobin. People who inherit two sickle-cell alleles, are seriously anaemic and usually die young. Those who are heterozygous are only mildly affected. (a) State the process that brought about the sickle-cell gene. Gene mutation (b) Suggest, with a reason, whether the allele, that gives rise to sickle-cell anaemia is dominant or recessive. The allele is recessive, because only those with two sickle-cell alleles (homozygous recessive) exhibit the trait, whereas those with only one sickle-cell allele (heterozygous) are less affected by the disease. (c) The diagram below shows a family tree with some members of a family being affected by sickle-cell anaemia. Key normal female normal male affected female I affected male 1 2 II 7 III 120 Worksheet 13 (S)SciBioWB_13.indd 120 3 ? 4 5 6 ? 8 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:12 PM Using ‘N’ to represent the normal allele and ‘n’ to represent the sickle-cell allele, (i) State the genotype(s) of persons 1 and 2. Explain your answer. The genotype of persons 1 and 2 is Nn, because one of their offspring, person 3, has sickle-cell anaemia. This means that person 3 must have inherited the sickle-cell allele from both 1 and 2. (ii) Person 4 often complains of becoming tired easily. He had his blood tested. What is the probability that he has sickle-cell anaemia? Use a genetic diagram to show this. Parental phenotypes Parental genotypes Gametes Offspring genotypes Offspring phenotypes Offspring phenotypic ratio Normal Nn Normal Nn ⴛ N n N n NN Normal Nn Normal Nn Normal nn Affected Normal : Affected = 3 : 1 Person 4 has a 25% chance of having sickle-cell anaemia. (d) Explain why a person with two sickle-cell alleles will usually die young. A person with two sickle-cell alleles, has abnormal haemoglobin in his or her red blood cells. The red blood cells have a shorter lifespan due to frequent changes in shape and clumping. Thus, there are insufficient red blood cells to carry the oxygen required for respiration around the body. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_13.indd 121 Worksheet 13 121 8/25/12 5:12 PM 3. A scientist analysed a double stranded DNA sample and found that 15% of its nitrogenous bases were adenine. Calculate the percentage of the other nitrogenous bases in the DNA sample. Explain your answer. According to the base pairing rule, adenine pairs with thymine, while guanine pairs with cytosine, hence, given that adenine is 15%, percentage of thymine = percentage of adenine = 15% 100% – (15% + 15%) 2 70% = 2 percentage of guanine = percentage of cytosine = = 35% Section C: Free-Response Questions Answer the following questions. 1. (a) Distinguish between (i) a gene and an allele; and A gene is a segment of DNA that stores hereditary information. Genes can have different forms, for example, a gene for height can code for ‘short’ or ‘tall’. Different forms of the same gene are called alleles, which occupy the same locus in homologous chromosomes. (ii) genotype and phenotype. Genotype is the genetic make-up of an organism, that is, the combination of genes in the organism, whereas phenotype is a result of genes and the effects of the environment on the organism. The genotype of an organism cannot be observed directly, whereas its phenotype, which is an expressed trait, is usually visible in its outward appearance. 122 Worksheet 13 (S)SciBWB_13.indd 122 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/27/12 5:36 PM (b) Draw a genetic diagram to show the inheritance of height in pea plants when two heterozygous tall pea plants cross. The tall allele is the dominant allele. Let T be the dominant allele (allele for tallness). Let t be the recessive allele (allele for dwarfness). Parental phenotype Parental genotype Gametes Offspring genotypes Offspring phenotypes Offspring phenotypic ratio 2. Tall Tt Tall Tt ⴛ T t T t TT Tall Tt Tall Tt Tall tt Short Tall : Short = 3 : 1 (a) Distinguish between continuous variation and discontinuous variation. Continuous variation deals with phenotypes that range from one extreme to the other. It is brought about by the additive effect of many genes. Continuous variation is affected by environmental conditions. Discontinuous variation is brought about by one or a few genes. This results in clear-cut phenotypes with no intermediate forms between traits. Unlike continuous variation, discontinuous variation is not affected by environmental conditions. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_13.indd 123 Worksheet 13 123 8/25/12 5:12 PM (b) Discuss the possible causes of mutation, and give an example where a mutation is advantageous to an organism. Mutation occurs when there is an error during the replication of the gene or the chromosome. It can also be caused by mutagens such as radiation and chemicals. The gene mutation that causes sickle-cell anaemia, may be advantageous in places where malaria is common. Individuals who are heterozygous for the sickle-cell allele, are more resistant to malaria, because a small percentage of their red blood cells are sickle-shaped (mutated). As these individuals do not fully contract either disease, they have better chances of surviving and reproducing, compared to homozygous or normal individuals. This selection ensures that the recessive allele persists in the population. WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives MultipleChoice Structured (a) Distinguish between "gene" and "allele" Textbook section(s) 1 16.2 1 16.2 1 16.2, 16.3 (b) Understand and explain commonly used terms in the study of heredity, such as "dominant", "recessive", "homozygous", "heterozygous", "phenotype" and "genotype" 1, 2 (c) Solve problems, and predict results of monohybrid inheritance using genetic diagrams 3, 4 1, 2 (d) Describe the determination of sex in humans with reference to the XX and XY chromosomes 5 1 (e) Differentiate between discontinuous and continuous variation 7, 8 (f) Describe different types of mutation and their causes 6 2 9, 10 3 17.1 3 17.1, 17.2 (g) State the composition of DNA and its structure (h) Understand and apply the rules of base pairing 124 FreeResponse Worksheet 13 (S)SciBWB_13_CS6.indd 124 16.3 2 16.4 2 16.5 © 2013 Marshall Cavendish International (Singapore) Private Limited 11/10/16 6:26 PM Class: Worksheet 14 ( Name: ) Date: CONNECT IT! Complete the concept map below using keywords learnt in the chapter. Discuss with your classmates how the concept map can be extended. affected by Ecosystem affect important processes Abiotic factors Biotic factors Human activity Non-cyclic energy flow e.g. Carbon cycle results in such as physical factors causes e.g. Pollution to maintain Biodiversity e.g. water pollution by Energy transfer Light between Temperature Sewage Water Inorganic wastes intensity availability Oxygen content can be compared using e.g. Conservation Industry Trophic levels Pyramid of numbers results in need for such as living things that interact with an organism Nutrient cycling for Chapters 18 and 19 Ecology and Our Impact on the Ecosystem Insecticide Salinity of soil/water pH of soil/water Ecological pyramids stored in fatty tissues of organisms Pyramid of biomass Bioaccumulation Producers Consumers Decomposers feeding relationship represented as Food chains © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_14.indd 125 interlinked to form Food web Worksheet 14 125 8/25/12 5:14 PM TEST IT! Section A: Multiple-Choice Questions Choose the correct answer, and write its letter in the brackets provided. 1. The diagram below shows a food web. aphid plant ladybird caterpillar snake small bird owl shrew badger Which one of the following organisms is a secondary consumer? A B C D 2. Aphid Caterpillar Small bird Snake ( C ) Which of the following pyramids represents the biomass of the organisms in the food chain below? tree aphid A B C D bird ( B ) 3. Producers are organisms that A B C D 126 break down dead organic matter can convert light energy into chemical energy live on or in the body of another organism obtain energy from other organisms on which they feed Worksheet 14 (S)SciBioWB_14.indd 126 . ( B ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:14 PM 4. Sewage pollutes water and causes a A B C D 5. in in in in the the the the carbon dioxide concentration dissolved nitrate concentration number of decomposers oxygen concentration ( D ) . Bioaccumulation refers to the build-up of A B C D 6. decrease decrease decrease decrease . biodegradable chemicals along a food chain energy along a food chain living organisms along a food chain non-biodegradable chemicals in an organism ( D ) A factory discharges sewage into a river at point P. Which of the following graphs illustrates the changes in the level of dissolved oxygen in an organism? A B Level of dissolved oxygen P C Distance from the source of the river P D Level of dissolved oxygen P Level of dissolved oxygen Distance from the source of the river Level of dissolved oxygen Distance from the source of the river P Distance from the source of the river ( C ) 7. Which types of microbes are used in the treatment of sewage? A B C D Aerobic bacteria, anaerobic bacteria and fungi Aerobic bacteria and anaerobic bacteria Aerobic bacteria and fungi Anaerobic bacteria and fungi © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_14.indd 127 ( A ) Worksheet 14 127 8/25/12 5:14 PM Section B: Structured Questions Answer the following questions in the spaces provided. 1. The pyramid of biomass for a forest is shown below. The width of each band represents the biomass of each trophic level. eagles sparrows caterpillars trees (a) What is the correct unit used to record biomass? It is kilogramme per unit area. (b) Why are pyramids of biomass more useful when studying a particular ecosystem than pyramids of numbers? Pyramids of numbers may be inverted if organisms of one trophic level are parasitic on organisms of another trophic level. Hence, it is not an accurate representation of the transfer of energy. (c) From the pyramid of biomass, identify (i) the producer; and Trees (ii) the secondary consumer. Sparrows (d) Insecticide was sprayed onto the trees to control the number of caterpillars. After a few years, scientists noticed that there was a decline in the number of eagles in the forest. Suggest two reasons for the decline. The first reason is that bioaccumulation had occurred. The insecticide had accumulated in the caterpillars’ bodies. When the sparrows ate the caterpillars, the insecticide further accumulated in the sparrows, as each sparrow fed on many caterpillars. When the eagles fed on the sparrows, more insecticide accumulated in the eagles. Hence, the eagles were poisoned. The second reason is that the decrease in caterpillars led to the decrease in sparrow population, as there was less food for the sparrows. This in turn resulted in less food for the eagles, causing the eagle population to decrease. 128 Worksheet 14 (S)SciBioWB_14.indd 128 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:14 PM 2. The diagram shows the energy and nutrient pathways in a food chain. The Sun energy pathways: CO2 nutrient pathway: Herbivores Energy lost Dead organic matter Producer Carnivores (a) What nutrient is illustrated in the nutrient pathway? Carbon (b) How does the producer take in energy from the Sun? It obtains energy from photosynthesis in which, chlorophyll absorbs light energy and converts it to chemical energy stored in the form of carbohydrate molecules. (c) Why do the arrows representing the energy pathway decrease in size from the Sun to the carnivores? This is because, energy is lost as heat, and some energy accumulates as biomass (mass of the organisms). Thus, only a small percentage of the energy is transferred to the next trophic level. (d) A carbon sink is an area that stores carbon compounds for an indefinite period. Name two natural places on Earth that can be considered as carbon sinks. Any two of the following: Soil/Forest/Ocean/Savanna © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_14.indd 129 Worksheet 14 129 8/25/12 5:14 PM 3. The diagram below shows the effects of human activities in a town. residential area forest river factory (a) Organic matter is discharged from the residential area. (i) State the effects of the discharge of this organic matter on the organisms in the river. The organic matter provides a rich source of food for bacteria in the river. This will increase the bacterial population, which uses up more oxygen from the river water. This in turn decreases the oxygen concentration in the water for other organisms. The other organisms in the river will die due to a lack of oxygen. (ii) How would the concentration of dissolved oxygen change as water flows downstream from the residential area? Further downstream, there is no more waste material being discharged into the river, which causes the bacterial population to decrease. Therefore, the dissolved oxygen concentration will increase. (b) The factory discharges high-temperature waste into the river. How would this discharge affect the organisms in the river? High-temperature waste will increase the temperature of the river. Since enzymes denature at high temperatures, important enzyme reactions that are essential for the survival of organisms, may not occur. Therefore, the populations of the river organisms will decrease. 130 Worksheet 14 (S)SciBioWB_14.indd 130 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:14 PM (c) Suggest two ways to improve the quality of water in the river. Firstly, treat the sewage before discharging it, to remove toxic materials and bacteria. Secondly, use microorganisms that can break down organic matter / toxic matter to pre-treat the waste water (bioremediation) before discharging it. LEARN & APPLY Go through the worked example below, then try to answer the Free-Response Questions. Worked Example Describe how humans cause water pollution. Thought Process Your answer should focus on the effects of human activities, and not on natural disasters. You should include a definition of pollution, the source of pollutants, and the effects of these pollutants on water. Answer Points to cover 1. Pollution is the addition of substances to the environment that damage it, making it undesirable or unfit for life. 2. Most of the time, pollution is brought about by human activities as the human population increases, and industrialisation and urbanisation become more prevalent. Waste materials from factories, and untreated sewage dumped into rivers and streams, pollute the water (sources of pollutants). 3. Waste materials from factories may include poisonous chemicals or metals such as mercury, lead, zinc and arsenic. They make the water unsafe to drink. It is also unsafe to consume fish living in such polluted water. 4. Untreated sewage, especially with high nitrate content, is a good source of food for bacteria. Bacteria grow and multiply rapidly, using up the oxygen in water. 5. Other organisms in the water such as fishes and plants die due to a lack of oxygen in the water (the bacteria also cause diseases such as cholera and typhoid — making the water unsafe to drink). 6. Nitrates, present in fertilisers that are leached into the river, may promote growth of algae (algal bloom). The algae will block sunlight for photosynthesis of underwater plants. As plants die and decompose, the level of oxygen decreases, and organisms such as fishes die. © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_14_CS6.indd 131 Worksheet 14 131 12/12/16 10:04 am Section C: Free-Response Questions Answer the following questions. Discuss the importance of 1. conserving species to maintain biodiversity; Biodiversity is a valuable natural resource. Conserving species prevents the extinction of species, and maintains a stable and balanced ecosystem. In addition, conservation is of scientific value, as the study of biodiversity provides useful information to humans. Many animals and plants are a major source of human food. Many organisms are sources of raw materials for industries, medicinal drugs and natural insecticides. Conserving species is also necessary to maintain a large gene pool, so that agricultural produce can be improved through cross-breeding different varieties of wild plants and animals with favourable genes. 2. proper treatment of sewage before discharge; and Untreated sewage may contain disease-causing bacteria that can potentially affect large human populations when it enters sources of drinking water. The organic waste in sewage are a good source of food for bacteria, leading to rapid increases in the bacterial population. As bacteria use up the oxygen in the water, other organisms die due to a lack of dissolved oxygen. Anaerobic bacteria continue breaking down organic waste and releasing bad-smelling gases in the process. Untreated sewage, if discharged into rivers or lakes, can also lead to eutrophication, causing the death of organisms due to a lack of oxygen. 3. controlling the use of pesticides. Uncontrolled use of pesticides can pollute water bodies and contaminate food sources when non-biodegradable pesticides are washed by rainwater into streams, rivers and lakes. These pesticides may accumulate in the bodies of consumers that feed on contaminated organisms, resulting in the pesticides being passed along the food chains. Eventually, humans and top consumers suffer the toxic effects due to bioaccumulation. In addition, the use of a pesticide in excess could promote the growth of populations of pests that develop resistance to the pesticide. 132 Worksheet 14 (S)SciBioWB_14.indd 132 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:14 PM WORK ON IT! Circle the question(s) you have answered incorrectly. Then, revisit the relevant section(s) in the textbook to strengthen your understanding of the key concept(s). Questions Learning objectives MultipleChoice (a) Describe the non-cyclical nature of energy flow (b) Understand and explain terms such as "producers", "consumers" and "trophic level" in the context of food chains and food webs 2 2 (e) Describe the carbon cycle and the role of carbon sinks (f) Describe and evaluate the effects of water pollution by sewage, inorganic waste and insecticides 4, 5, 6 (g) Outline the role of microorganisms in sewage treatment 7 (h) Discuss reasons for conservation of species © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWB_14_CS6.indd 133 Textbook section(s) 18.4, 18.5 1, 3 (c) Explain how energy losses occur along food chains, and the efficiency of energy transfer between trophic levels (d) Interpret pyramids of numbers and biomass Structured FreeResponse 18.4 2 18.5 1 18.5 2 18.6, 18.7 1, 3 2, 3 19.1 19.1 1 19.2 Worksheet 14 133 11/10/16 6:27 PM (S)SciBioWB_14.indd 134 8/25/12 5:14 PM Class: Model Examination Paper ( Name: ) Date: Section A: Multiple-Choice Questions Answer ALL the questions in this section. For each question, there are four possible answers, A, B, C and D. Choose the correct answer, and write its letter in the brackets provided. 1. When comparing a plant cell to an animal cell, one would expect to find A B C D 2. B C D Carbon Carbon Carbon Carbon monoxide and irritants monoxide and nicotine monoxide and tar monoxide, nicotine and tar ( B ) To produce male sex hormones that are responsible for the development of male sexual characteristics To secrete a fluid that activates the eggs in females To secrete a fluid that contains enzymes, which break down the egg membrane To secrete a slippery fluid that contains nutrients and enzymes, which nourish and activate sperms ( D ) Which of the following correctly matches the substance with its process of entry into an epithelial cell of the small intestine after a heavy meal? Substance Process A Amino acids Osmosis B Fats Active transport C Glucose Diffusion D Water Diffusion © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_ExamPaper.indd 135 ( C ) What is the role of the prostate gland, the Cowper’s gland and the seminal vesicles in the male reproductive system? A 4. a cellulose cell wall in both the plant and animal cells a nucleus containing a nucleolus in the animal cell, but not in the plant cell endoplasmic reticulum in both the plant and animal cells ribosomes in the animal cell, but not in the plant cell Which of the following substances in cigarette smoke cause an increased chance of fat deposits in the coronary arteries? A B C D 3. . ( C ) Model Examination Paper 135 8/25/12 5:16 PM For questions 5 and 6, refer to the graph of an enzyme-catalysed reaction shown below. Rate of reaction X Y Substrate concentration 5. Which of the following best describes what happens during interval X? A B C D 6. Which of the following best describes what happens during interval Y? A B C D 7. Product formation has stopped. Some of the active sites are unoccupied. The amount of product being formed is equal to the amount of product being broken down. The product is being formed at a constant rate. ( B ) Product formation has stopped. Some of the active sites are unoccupied. The amount of product being formed equals the amount of product being broken down. The product is being formed at a constant rate. ( D ) An experiment was conducted to trace the path taken by carbohydrate molecules from a plant, after a herbivore had consumed the plant. The carbohydrate molecules were labelled with radioactive carbon. Special equipment were then used to detect these radioactively labelled molecules. Which of the following paths is correct? In the plant 8. A Glucose starch Glucose maltose starch B Glucose starch Maltose glucose glycogen C Starch maltose Glucose maltose starch D Starch maltose Starch glucose glycogen ( B ) A patient had his gall bladder removed, and needs to be put on a special diet. Which menu would be most suitable for this patient? A B C D 136 In the herbivore Boiled chicken meat (without skin) and a fresh orange Chicken sausages and cream cake Fried fish fillet and boiled potato Pan-fried fish fillet in cheese sauce and green salad Model Examination Paper (S)SciBioWB_ExamPaper.indd 136 ( A ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:16 PM 9. The diagram below shows part of the digestive system. X Y Z Which structures produce substances involved in fat digestion? A B C D X only Y only X and Y only X, Y and Z ( C ) 10. A particular characteristic is controlled by a dominant allele, R, and a recessive allele, r. Which of the crosses will give a 1:1 ratio of two phenotypes among the offspring? A B C D RR × rr Rr × RR Rr × Rr Rr × rr ( D ) 11. A plant was placed in a bell-jar in a warm and brightly-lit environment. The air in the jar contains carbon dioxide, where carbon exists as a radioactive isotope. 1 3 2 After six hours, which of the following regions are likely to have high levels of radioactivity? A B C D 1 and 2 1 and 3 2 and 3 1, 2 and 3 ( C ) 12. DNA molecules, isolated from a rat cell and a human cell, are found to differ in the sequence of . their A B C D bases sugars phosphates bases, sugars and phosphates © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_ExamPaper.indd 137 ( A ) Model Examination Paper 137 8/25/12 5:16 PM For questions 13 and 14, refer to the diagram below, which shows a vertical section of the human heart. 2 4 3 5 1 Y W X Z 13. Which of the following blood vessels contains blood with the highest oxygen concentration? A B C D 1 2 3 5 ( D ) 14. Heart sounds are produced by A B C D . closing of valves W and X closing of valves W, X, Y and Z opening of valves Y and Z opening of valves W, Y and Z ( B ) 15. The graph below shows the concentration of lactic acid in the blood of an athlete. During which time period is oxygen debt incurred and repaid? Concentration of lactic acid P Q R Time 138 Oxygen debt incurred Oxygen debt repaid A P Q and R B Q R C P and Q R D Q and R P Model Examination Paper (S)SciBWBTE_ExamPaper.indd 138 ( B ) © 2013 Marshall Cavendish International (Singapore) Private Limited 2/12/16 10:14 am 16. The diagram below shows the structure of a flower. X Y W Z At which part of the flower can the male and female gametes be found, assuming that there is no pollination or fertilisation? Male gamete Female gamete A W X B X Y C Y Z D Z Y ( C ) 17. The diagram below shows the male reproductive and urinary system. What is the tube labelled X? X A B C D Epididymis Seminal vesicle Sperm duct (vas deferens) Ureter © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_ExamPaper.indd 139 ( C ) Model Examination Paper 139 8/25/12 5:16 PM 18. The following family tree shows the inheritance pattern of an inheritable disease. Key normal male affected male normal female affected female P What is/are the possible genotype(s) of P, who is normal (B represents the normal allele and b represents the allele for the disease)? A B C D BB bb Bb BB, Bb ( C ) 19. The rule of complementary base pairing in DNA states that A B C D adenine adenine adenine adenine pairs pairs pairs pairs with with with with . cytosine, while guanine pairs with thymine guanine, while cytosine pairs with thymine thymine, while guanine pairs with cytosine thymine, while guanine pairs with thymine ( C ) 20. The graph shows the populations of animal X and animal Y in the same habitat, from 1920 to 1940. Number of individuals/ hundreds 80 0 X 60 0 Y Y 40 0 20 0 1925 1930 1935 1940 Year What biological relationship between animal X and animal Y is shown in the graph? A B C D 140 X X X X and and and and Y Y Y Y compete for the same, limited resource. interact such that one of them benefits, while the other is harmed. interact such that one is the predator, and the other is the prey. interact such that both benefit. Model Examination Paper (S)SciBioWB_ExamPaper.indd 140 ( C ) © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:16 PM Section B: Structured Questions Answer ALL questions in the spaces provided. 1. The diagram below shows a simplified representation of the transfer of energy in a generalised ecosystem. Each box represents a category of organisms, grouped together based on their trophic position in the ecosystem. The Sun Sun I Decomposers II III (a) State the trophic levels of the organisms in boxes I, II and III. [3] Box I is the producer. Box II is the primary consumer. Box III is the secondary consumer. (b) State the form of energy that enters organisms in box III. [1] It is chemical energy. (c) Identify which arrow represents the greatest transfer of energy per unit time (add a large ‘ ’ next to the arrow). [1] (d) Explain what the wavy arrows leaving each box represents. [3] The wavy arrows represent the energy [1] that is lost as heat [1] due to metabolic activities [1] such as respiration. (e) Explain why there are usually not many trophic levels in a food chain. [2] This is because energy is lost as it is passed from one trophic level to the next. Usually about 10% of the energy [1] is passed down from one level to the next. If there are too many trophic levels, the higher trophic levels will receive very little energy [1] for survival. [Total: 10 marks] © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_ExamPaper.indd 141 Model Examination Paper 141 8/25/12 5:16 PM 2. The diagram below shows the transverse section of a leaf. upper epidermis palisade mesophyll cell spongy mesophyll cells lower epidermis guard cells (a) Label on the diagram, the different types of cells that can be found in the transverse section of a leaf [5] (b) Draw a small circle in each of the cells in (a) that contains chloroplasts. [3] Circle should be drawn in the palisade mesophyll cells [1], spongy mesophyll cells [1] and guard cells [1]. (c) State two internal features of a leaf, and explain how these features allow the leaf to photosynthesise more efficiently. [4] Any two of the following: Presence of chloroplasts [1] in mesophyll cells and guard cells for photosynthesis to occur / Guard cells forming stomata [1] for gas exchange [1] / Presence of vascular bundles [1] to transport water to mesophyll cells for photosynthesis [Total: 12 marks] 3. Four cylinders of potato (with a length of 20 mm as shown below) were each placed in sugar solutions of different concentrations. 20 mm After one hour, the cylinders were removed and gently blotted dry, before their lengths were measured again. The results were recorded and shown in the table below. 142 Solution Original length/mm Final length/mm A 20 25 B 20 20 C 20 15 D 20 18 Model Examination Paper (S)SciBWBTE_ExamPaper.indd 142 © 2013 Marshall Cavendish International (Singapore) Private Limited 11/10/16 6:28 PM (a) Which movement mechanism is being investigated in this experiment? [1] Osmosis (b) Which property of the potato cells allows the movement mechanism in (a) to happen? [1] Partially permeable membrane (c) Explain why the length of the cylinders will (i) remain unchanged; [2] Water potential of the cell sap is the same as the environment [1]; hence, there is no net movement of water. [1] (ii) increase; and [2] Water potential of the environment is higher than the water potential of the cell sap [1]; hence, water moves from the environment into the cell sap by osmosis. [1] (iii) decrease. [2] Water potential of the environment is lower than the water potential of the cell sap [1]; hence, water moves from the cell sap into the environment by osmosis. [1] (d) Suggest and explain, which solution is likely to have the same water potential as the potato cells. [2] Solution B. [1] There was no change in the length of the cylinder. [1] (e) State whether solution A is a dilute or concentrated sugar solution. [1] Dilute sugar solution [Total: 11 marks] © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_ExamPaper.indd 143 Model Examination Paper 143 8/25/12 5:16 PM 4. The diagram below shows the structure of a developing human fetus and part of the uterine wall. B A X Content in A (arbitrary units) Content in B (arbitrary units) • 20 units of oxygen • 40 units of carbon dioxide in the form of hydrogencarbonate ions • 36 units of glucose • 32 units of urea • 50 units of oxygen • 25 units of carbon dioxide in the form of hydrogencarbonate ions • 78 units of glucose • 12 units of urea (a) Identify vessels A and B. [2] A is the umbilical artery, B is the umbilical vein. (b) State the reasons for your answer in (a). [2] The umbilical artery carries deoxygenated blood (lower oxygen concentration) and waste products (higher concentrations of carbon dioxide and urea) away from the fetus. [1] On the other hand, the umbilical vein carries oxygenated blood (higher oxygen concentration) and food substances (higher concentration of glucose) to the fetus. [1] (c) The function of X is to support and protect the fetus. X contains fluids known as the amniotic fluid. Suggest how X performs its functions. [2] It provides buoyancy / acts as a shock absorber. [1] It protects the fetus against mechanical injury. [1] [Total: 6 marks] 144 Model Examination Paper (S)SciBioWB_ExamPaper.indd 144 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:16 PM 5. The action of lipase on fats in three different milk solutions, A, B and C, was investigated. The volumes of enzyme and milk solutions were kept constant. Lipase breaks down fats into fatty acids and glycerol. The amount of fatty acids formed can be deduced by measuring the pH of the mixture. The graph below illustrates the results obtained. pH of reaction mixture A B C A, 40°C Sample A, 30˚C C, 40°C X B, 40°C 0 2 4 6 8 10 12 14 16 18 20 (a) State the time taken for the reaction to be completed in milk sample C. Time/min [1] 6 minutes (b) Comparing B and C, suggest a factor that could have affected the rate of reaction between 0 minute and 2 minutes. [1] It is the concentration of substrate (fat). (c) Suggest why the graphs labelled A and C became level at the same height, X. [2] The amounts of fats (substrate) in A and C are the same. [1] The graphs become level when all the fats in the milk solutions are broken down by lipase into fatty acids and glycerol. [1] (d) On the graph given, draw and label the graph that would be obtained if the same volume of lipase was added to the same volume of sample A, with the reaction carried out at a temperature of 30°C. [2] [Total: 6 marks] © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBioWB_ExamPaper.indd 145 Model Examination Paper 145 8/25/12 5:16 PM Section C: Free-Response Questions Answer 2 out of 3 questions. Write your answers on the writing paper provided. Label your answers prominently and neatly. Use a fresh sheet of paper for each question. Hand in Section C separately from Section B. 1. Compare and contrast the transport of nutrients and water in plants and humans. [10] [Total: 10 marks] 2. (a) (i) Define aerobic respiration and photosynthesis. (ii) Explain the relationship between these two processes, and their importance to living organisms. [5] (b) Gas exchange occurs at the surface of both mesophyll cells in the leaf and at the alveoli in the lungs. In what ways are the structures and functions of these surfaces similar for gas exchange? [3] (c) Phytoplankton are microscopic food producers. Only a small percentage of sunlight reaching a pond is used by phytoplankton for photosynthesis. Suggest two reasons for this. [2] [Total: 10 marks] 3. Draw a labelled diagram of a cross-section of a dicotyledonous leaf, and use your diagram to describe the movement of water inside a leaf. [10] [Total: 10 marks] 1. Similarities: Both transport systems consist of vessels for transporting substances, [1] and the transport medium is liquid/fluid. [1] Differences: Plants have a closed circulatory system driven mainly by transpirational pull in xylem, whereas humans have a closed circulatory system driven by the pumping of the heart. [2] Transport vessels in plants include xylem vessels, which are dead cells, and phloem tissue. No organ is involved. Transport vessels in humans include arteries, veins and capillaries, which are all tissues. An organ, the heart, acts as a pump. [2] In plants, water and dissolved ions are transported in the xylem. Manufactured food is transported by the sieve tubes of the phloem. The different nutrients needed by plants are, thus, transported in separate vessels. In humans, digested food substances, ions and water are all transported in the blood. Thus, nutrients are not carried in separate vessels. [2] The phloem conducts food materials in the form of sucrose and amino acids, from the green parts of the plant, especially the leaves, to other parts of the plant. The xylem conducts water and dissolved mineral salts from the roots to the stems and leaves. In humans, blood transports the following: digested food substances from blood capillaries in the intestines to all parts of the body; excretory products from tissues to excretory organs; heat from respiring tissues to all parts of the body; and oxygen from the lungs to all parts of the body. [2] 146 Model Examination Paper (S)SciBioWB_ExamPaper.indd 146 © 2013 Marshall Cavendish International (Singapore) Private Limited 8/25/12 5:16 PM Notes 2. (a) (i) Aerobic respiration is the breakdown of food substances in the presence of oxygen with the release of a large amount of energy, and carbon dioxide and water as waste products. [1] Photosynthesis is the process in which light energy absorbed by chlorophyll is transformed into chemical energy used to synthesise carbohydrates from water and carbon dioxide. Oxygen is released in the process. [1] (ii) In photosynthesis, light energy is transformed into chemical energy stored in carbohydrates, while in aerobic respiration, chemical energy stored in food such as carbohydrates is released in the form of heat energy and chemical energy. [1] Photosynthesis provides substrates for organisms to use in tissue respiration. It provides energy in a usable form for all organisms. [1] Aerobic respiration releases useful energy from food for organisms to carry out vital activities. [1] (b) Both mesophyll cells and alveoli are covered with a thin film of moisture to allow gases to dissolve. [1] Carbon dioxide diffuses rapidly into mesophyll cells in plants, while oxygen diffuses into alveoli through one-cell thick alveolar walls. [1] Both mesophyll cells and alveoli have large surface areas to increase the rate of diffusion. [1] (c) Energy from the sun is lost to the surroundings by reflection from the pond surface, conduction by water in the pond and convection from the pond surface. [1] Light energy may be absorbed by the water and converted into heat energy. [1] 3. Water continuously moves out of the mesophyll cells to form a thin layer of moisture around the cells. [1] As water evaporates from the thin film of moisture, it enters the intercellular air spaces. [1] From there, water vapour diffuses through the stomata to the drier air outside the leaf. [1] As water evaporates from the mesophyll cells, the water potential in their cell sap decreases. [1] They begin to absorb water by osmosis from the cells deeper inside the leaf. [1] These cells have a lower water potential than the adjacent xylem vessels. Therefore, water passes from xylem vessels into the surrounding mesophyll cells by osmosis. [1] This creates the transpiration pull, which helps to draw mineral salts and water from the roots up the plant. [1] Drawing [1] Labels [2] palisade mesophyll cell xylem spongy mesophyll cell intercellular air space stoma © 2013 Marshall Cavendish International (Singapore) Private Limited (S)SciBWBTE_ExamPaper.indd 147 Notes Model Examination Paper 147 21/12/16 2:20 pm (S)SciBioWB_ExamPaper.indd 148 8/25/12 5:16 PM (S)SciBioWB_ExamPaper.indd 149 8/25/12 5:16 PM (S)SciBioWB_ExamPaper.indd 150 8/25/12 5:16 PM (S)SciBioWB_ExamPaper.indd 151 8/25/12 5:16 PM (S)SciBioWB_ExamPaper.indd 152 8/25/12 5:16 PM 2nd Edition 2nd Edition The Discover Biology for GCE 'O' Level Science Workbook (2nd Edition) is specially developed to complement the Discover Biology for GCE ‘O’ Level Science (2nd Edition) textbook. Designed to support learning with understanding, this workbook is written in line with the 2013 GCE Ordinary Level Science (Biology) syllabus from the Ministry of Education, Singapore. Its wide-ranging exercises build foundational knowledge and develop critical higher-order thinking skills. Workbook (Teacher’s Edition) Reinforces concept development • Concept mapping helps students review and integrate key concepts taught in a chapter. 2nd Edition Shapes independent learners and critical thinkers • Self-evaluation tool at the end of every worksheet helps students monitor their learning progress. • A variety of context-based and data-based questions challenge students to train up their higher-order thinking skills. Builds skills and exam confidence • Thought Process in worked examples guides students through essential points to consider when answering free-response questions. • A Model Examination Paper gives further practice and boosts exam confidence by helping students familiarise with the exam format. Lam Peng Kwan • Eric Y K Lam Christine Y P Lee Other components • Practical Book • Textbook Lam Peng Kwan Eric Y K Lam Christine Y P Lee ISBN 978-981-01-1730-6 Workbook Teacher's Edition (S)SciB_WB&TE Cover.indd 1 9/24/12 10:17 AM