Saif Ur Rehman
Student ID# 110132667
Laboratory Experiment Tile: Introduction Assignment
Discussion Question 1
Discuss the trend in UV-Vis absorbance intensity with increasing putrescine
concentration.
On the basis of the UV-Vis spectroscopic experiment performed, it was found that with
increasing concentration of putrescine, the Absorbance intensity also experienced a gradual
increase and a linear plot was observed. This is because absorbance intensity depends upon the
concentration of putrescine. The colour intensity of the solution also increases with an increase
in concentration.
This system is accurate and precise but requires more time and modern equipment. A better
approach is to design special strips that indicate an instant colour change. It could be more
economical and easier.
Название диаграммы
80
Absorbance (a.u.)
70
60
50
40
y = 430,05x + 2,2413
R² = 0,9906
30
20
10
0
0
0,01
0,02
0,03
0,04
0,05
0,06
Putrescine Concentration (mol/L)
Discussion Question 2
0,07
0,08
0,09
Compare the change in absorbance of the sensors on a meat package in the
freezer compared to the meat package left out at room temperature over 3
days. Briefly state how you would interpret these results.
Meat package stored under different conditions gives different results. Colour intensity was
measured every 6 Hours for a period of 3 days.
The meat package stored at room temperature gradually increased absorbance over time. This is
because putrescine concentration increased as the meat was placed at room temperature.
However, a negligible colour change was observed in the meat stored in the freezer because no
putrescine was produced.
40
Absorbance (a.u.)
35
30
25
20
15
10
5
0
0
10
20
30
40
50
60
70
80
Time (Hours)
Freezer
Room Temperature
Question 3
Calculate the putrescine concentration in the meat sample left at room
temperature after 24 hours.
The concentration of Putrescine sample left at Room Temperature after 24 hours:
𝑦 = 𝑚𝑥 + 𝑐
10.123 = 430.05𝑥 + 2.2412
𝑥 = 0.0183