SCIENCE QUARTER 4 – MODULE 2 (Week 3-4) Four Categories of Biomolecules 1 What I Need to Know Food is one of the basic needs of living organisms. Different types of food give us different nutrients. For instance, grains provide carbohydrates for energy; egg gives proteins for growth and repair and so on. These compounds belong to the four main categories of biomolecules; carbohydrates, proteins, lipids and nucleic acids. This module will provide you with information and simple activities that will help you understand the four main categories of biomolecules. After going through this module, you are expected to: 1. identify the major categories of biomolecules such as carbohydrates, lipids, proteins and nucleic acids (S10MT-IVc-d-22) 2. differentiate the biomolecules from each other in terms of their structure and function; 3. recognize the importance of the four categories of molecules in our body What I Know Directions: Read each item carefully. Write only the letter of the correct answer for each question. Use a separate sheet for your answers. 1. Which is NOT a major source of protein? 2. 3. 4. 5. 6. 7. A. egg C. milk B. fish D. vegetable The most abundant monosaccharide in nature is . A. glucose C. maltose B. lactose D. sucrose Cholesterol is a/an . A. essential oil C. steroid B. saturated fat D. unsaturated fat Which has the most lipids? A. banana C. cheese B. champorado D. olive oil Which is a polysaccharide? A. glycogen C. fructose B. glucose D. lactose DNA is unique among molecules because it can . A. replicate itself B. come apart and reform C. form multiple polymer complex D. withstand very high temperatures These are the major constituent of the plasma membrane. A. Fats C. Phospholipids B. Oils D. Steroids 2 8. These are produced by the living cells that serve as catalyst in biochemical reactions. 9. 10. 11. 12. 13. 14. 15. A. amino acids C. hormones B. enzymes D. polypeptide The monomers that make up proteins are . A. amino acids C. glucose B. fatty acids D. nucleotides These are chemical signaling molecules secreted by the endocrine glands. A. amino acid C. hormones B. enzymes D. nucleic acid These are macromolecules that are the key in the continuity of life. A. Carbohydrates C. Nucleic acids B. Lipids D. Proteins These are the monomers of nucleic acids. A. DNA C. Polynucleotide B. Nucleotide D. RNA Cellulose and starch are examples of . A. Disaccharide C. Oligosaccharide B. Monosaccharide D. Polysaccharide How many amino acids are there? A. 20 B. 21 C. 22 D. 23 Which polysaccharide is stored in the liver and the muscles? A. Chitin B. Cellulose C. Glycogen D. Starches 3 Lesson 1 Four Categories of Biomolecules What’s In Directions: Find all the hidden BIOMOLECULE words in the word search puzzle below. Words can be spelled forward, backward, downward and upward. The keywords are listed in the pool of words written after the puzzle. Amino Acid Carbohydrates Cellulose Cholesterol Deoxyribonucleic acid Enzyme Glucose Hormones Lipids Monosaccharide Nucleic acids Nucleotides Phospholipids Proteins Steroid What’s New Carbohydrates, lipids, proteins and nucleic acids are the four main categories of biomolecules. These macromolecules are composed of smaller components called monomers. 4 Activity 1: Biomolecule Building Blocks Below is a graphic organizer. Write on each column the different building blocks or monomers of each biomolecules. Choose from the pool of words below. Amino acids Fatty acids CARBOHYDRATES Glucose PROTEINS LIPIDS Nucleotides NUCLEIC ACIDS What is It Biomolecules are the most essential organic molecules, which are involved in the maintenance and metabolic processes of living organisms. These non-living molecules are the actual foot-soldiers of the battle of sustenance of life. They range from small molecules such as primary and secondary metabolites and hormones to large macromolecules like proteins, nucleic acids, carbohydrates and lipids. Carbohydrates Carbohydrates are an essential part of our diet; grains, fruits, and vegetables are all natural sources of carbohydrates. Carbohydrates provide energy to the body, particularly through glucose, a simple sugar. Carbohydrates also have other important functions in humans, animals, and plants. Carbohydrates can be represented by the formula (CH2O)n, where n is the number of carbon atoms in the molecule. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. Carbohydrates are classified into three subtypes: monosaccharides, disaccharides, and polysaccharides. Monosaccharides are simple sugars, examples are glucose, which is the most abundant, galactose (part of lactose, a milk sugar) and fructose (found in fruit). Disaccharides form when two monosaccharides undergo a dehydration reaction (a reaction in which the removal of a water molecule occurs). Common disaccharides include lactose (found in milk, composed of galactose and glucose), maltose (malt sugar), and sucrose (table sugar, composed of glucose and fructose). A long chain of monosaccharides linked by 5 Figure 1: Glucose, galactose, and fructose have the same chemical formula but slightly different structures. covalent bonds is known as a polysaccharide. Starch, glycogen, cellulose, and chitin are examples of polysaccharides. Lipids Lipids are insoluble biomolecules, defined by an overall lack of polarity necessary for solubility in water-based solutions. In popular culture, fats are synonymous with lipids, giving lipids a negative role in diet and health. However, lipids play vital roles in many cellular processes including energy storage, structural support, protection, and communication. Common lipid groups include waxes, steroids, fats and oils, and phospholipids. Fats and oils are the most abundant lipids and are also called triglycerides. Triglycerides are lipids that store energy. It is composed of two main components – glycerol and fatty acids. Fatty acids can be saturated or unsaturated. Saturated fats have Figure 2: Structure of Saturated and straight carbon chains because they only Unsaturated Fatty Acid contain single carbon-carbon bonds. Saturated fats pack together closely and are solid at room temperature. Saturated fats are typically found in animal products. Butter is a good example. Unsaturated fats have a kink in their chain caused by a double bond or even a triple bond between carbons. Because of these kinks, unsaturated fats can't pack together very closely, making them liquid at room temperature. They are typically found in plant products. Vegetable oil is a good example. The hydrocarbon chains of both Source: saturated and unsaturated fats are attached https://dlc.dcccd.edu/biolo gy1-3/lipids to a carboxylic acid functional group. This is what makes them fatty acids. Phospholipids are the major constituent of the plasma membrane. It protects the cell by providing a selective barrier that regulates movement of molecules between the inside and outside of the cell A phospholipid has both hydrophobic and hydrophilic regions. The fatty acid chains are hydrophobic and exclude themselves from water, whereas the phosphate is hydrophilic and interacts with water. Cells are surrounded by a membrane, which has a bilayer of phospholipids. The fatty acids of phospholipids face inside, away from water, whereas the phosphate group can face either the outside environment or the inside of the cell, which are both aqueous. Unlike the phospholipids and fats discussed earlier, steroids have a ring structure. Although they do not resemble other lipids, they are grouped with them because they are also hydrophobic. All steroids have four, linked carbon rings and several of them, like cholesterol, have a short tail. Cholesterol is a steroid. Cholesterol is mainly synthesized in the liver and is the precursor of many steroid hormones, such as testosterone and estradiol. It is also the precursor of vitamins E and K. Cholesterol is the precursor of bile salts, which help in the breakdown of fats and their subsequent absorption by cells. Although cholesterol is often spoken of in negative terms, it is necessary for the proper 6 functioning of the body. It is a key component of the plasma membranes of animal cells. Waxes are made up of a hydrocarbon chain with an alcohol (–OH) group and a fatty acid. Examples of animal waxes include beeswax and lanolin. Plants also have waxes, such as the coating on their leaves, that helps prevent them from drying out. Proteins Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective; they may serve in transport, storage, or membranes; or they may be toxins or enzymes. The functions of proteins are very diverse because there are 20 different chemically distinct amino acids that form long chains, and the amino acids can be in any order. For example, proteins can function as enzymes or hormones. Enzymes, which are produced by living cells, are catalysts in biochemical reactions (like digestion) and are usually proteins. Enzymes can function to break molecular bonds, to rearrange bonds, or to form new bonds. An example of an enzyme is salivary amylase, which breaks down amylose, a component of starch. Hormones are chemical signaling molecules, usually proteins or steroids, secreted by an endocrine gland or group of endocrine cells that act to control or regulate specific physiological processes, including growth, development, metabolism, and reproduction. For example, insulin is a protein hormone that maintains blood glucose levels. Proteins have different shapes and molecular weights; some proteins are globular in shape whereas others are fibrous in nature. For example, hemoglobin is a globular protein, but collagen, found in our skin, is a fibrous protein. Protein shape is critical to its function. Changes in temperature, pH, and exposure to chemicals may lead to permanent changes in the shape of the protein, leading to a loss of function or denaturation. All proteins are made up of different arrangements of the same 20 kinds of amino acids. Amino acids are the monomers that make up proteins. Each amino acid has the same Figure 3: Amino acids are fundamental structure, which consists of a central carbon atom bonded to an amino group (–NH2), a made up of a central carbon carboxyl group (–COOH), and a hydrogen atom. bonded to an amino group (– Every amino acid also has another variable atom or NH2), a carboxyl group (– group of atoms bonded to the central carbon atom COOH), and a hydrogen atom. known as the R group. The R group is the only The central carbon’s fourth difference in structure between the 20 amino acids; bond varies among the otherwise, the amino acids are identical. different amino acids. The sequence and number of amino acids ultimately determine a protein’s shape, size, and Source: function. Each amino acid is attached to another https://opentextbc.ca/biology amino acid by a covalent bond, known as a peptide /chapter/2-3-biologicalbond. The products formed by such a linkage are molecules/ called polypeptides. 7 Nucleic Acids Nucleic acids are key macromolecules in the continuity of life. They carry the genetic blueprint of a cell and carry instructions for the functioning of the cell.The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material found in all living organisms, ranging from singlecelled bacteria to multicellular mammals. The other type of nucleic acid, RNA, is mostly involved in protein synthesis. The DNA molecules never leave the nucleus, but instead use an RNA intermediary to communicate with the rest of the cell. Other types of RNA are also involved in protein synthesis and its regulation. DNA and RNA are made up of monomers known as nucleotides. Each nucleotide is made up of three components: a nitrogenous base, a pentose (fivecarbon) sugar, and a phosphate group. Each nitrogenous base in a nucleotide is attached to a sugar molecule, which is attached to a phosphate group. DNA has a double-helix structure. It contains the sugar deoxyribose. It is composed of nitrogenous bases adenine (A), thymine (T), cytosine (C) and guanine (G). These nitrogenous bases pair together and follows a rule: cytosine pairs with guanine while adenine pairs with thymine. These base pairs are held together by hydrogen bonds. https://www.thesciencehive.co.uk/nucleotidesand-nucleic-acids RNA, on the other hand has a single-helix structure. The sugar that forms its backbone is called ribose. The nitrogenous bases for RNA are adenine, guanine, cytosine and uracil. As for the base pairing, cytosine (C) pairs with guanine (G) and adenine (A) pairs with uracil (U) What’s More Activity 1. CARBS Identification. For each molecule below, determine if it is a monosaccharide, a disaccharide, or a polysaccharide. Write your answer on the space provided for every item. 1. 2. 3. 4. 5. Fructose Cellulose Glucose Sucrose Glycogen _________________ _________________ _________________ _________________ _________________ 6. 7. 8. 9. 10. 8 Starch Maltose Lactose Chitin Ribose ___________________ ___________________ ___________________ ___________________ ___________________ Assessment 1 Directions: Read each item carefully. Write only the letter of the correct answer for each question. Use a separate sheet for your answers. 1. Carbohydrates are organic compounds made up of 2. 3. 4. 5. A. Carbon and hydrogen B. Carbon, hydrogen and oxygen C. Carbon, hydrogen, oxygen and sulfur D. Carbon, hydrogen, oxygen and nitrogen The most common monomer of carbohydrate is __________. A. amino acid B. glucose C. lactose D. nucleotide All of the following are polysaccharides EXCEPT A. cellulose B. glucose C. glycogen D. starch Which is the simplest form of carbohydrates? A. Disaccharide C. Oligosaccharide B. Monosaccharide D. Polysaccharide Which is a correct pair? A. disaccharide: fructose C. polysaccharide: cellulose B. monosaccharide: sucrose D. polysaccharide: glucose Activity 2. FACT OR BLUFF ON LIPIDS Directions: Write FACT if the statement is correct and BLUFF if the statement is wrong on the space/column provided for every item. No. 1 2 Statements Fact or Bluff Lipids are insoluble molecules. 3 Lipids are important in providing energy aside from carbohydrates. Butter is an example of unsaturated fats. 4 Fats and oils are the most abundant lipids. 5 Waxes are the major component of the plasma membrane. All saturated hydrocarbons contain single bonds and they are produce only by animals. Unsaturated hydrocarbons contain one or more double bonds. Vegetable oil, corn oil, and olive oil are produced by plants. Cholesterol is a precursor of vitamins A and K. Unsaturated fats are solid at room temperature. 6 7 8 9 10 9 Assessment 2 1. Fats and oils are also known as _______? A. Peptides B. Polymers C. Polypeptides D. Triglycerides 2. Which of these are not lipids? A. Fats B. Oils C. Proteins D. Waxes 3. Lipids are insoluble in water because lipid molecules are ____________. A. hydrophilic B. hydrophobic C. neutral D. polar 4. What is the molecular formula for cholesterol? A. C37H45OH B. C27H45OH C. C25H47OH D. C25H45OH 5. What is the reason why trans fats are harmful to our health? Trans fats___________. A. make the blood viscous. B. contain harmful artificial preservatives. C. have been altered chemically which make it dangerous. D. have a long shelf life and stay in the bloodstream longer than other lipids Activity 3. WORD PUZZLE ON PROTEIN Word Scramble. Unscramble the letters to find the PROTEIN words from the list below. 1. MEENZY - a protein that acts as a biological catalyst 2. OMAIN DICAS – building blocks of protein 3. DIONTATURAEN – the alteration of a protein 4. LEPTIPODEYP – long chains of amino acids 5. ALAMYSE – an enzyme that breaks down starch 6. GEG – food rich in protein 7. SOROMNEH – chemical signaling molecules 8. NEGOLLCA – a fibrous protein 9. NUSILIN – a hormone that maintains blood glucose levels 10. TIPDEEP DONB – a bond that holds the amino acids together. Assessment 3 Modified True or False. Write T if the statement is true. If the statement is false, change the underlined word or phrase to make the statement true. Write the correct word on the blank. ____________1. Proteins are made up of carbon, hydrogen, oxygen, nitrogen and sulfur. ____________2. There are 22 amino acids found in human protein. ____________3. Amino acid is the building block of protein. ____________4. Amino acids are joined together by a hydrogen bond. ____________5. Collagen is a globular protein. 10 Activity 4. DNA and RNA Analogy. Complete the following. 1. DNA: deoxyribose; RNA: _______________ 2. Adenine: thymine; _______________: guanine 3. DNA: _______________; RNA: cytoplasm 4. RNA: single stranded; DNA: _______________ 5. Thymine: DNA; _______________: RNA 6. _______________: nucleic acid; amino acid: protein 7. DNA: _______________; RNA: ribonucleic acid 8. nitrogenous bases: _______________; amino acids: peptide bond 9. _______________: hereditary traits; carbohydrates: energy 10. DNA; adenine, thymine, cytosine and guanine; _______________: adenine, uracil, cytosine and guanine What I Have Learned Great job! You are almost done with this module. Let’s summarize what you have learned from the lesson and activities by filling in the blanks. Use a separate sheet of paper and write only your answer. Biomolecules are substances that are produced by cells and living organisms. It consists of four major types: carbohydrates, lipids, proteins and nucleic acids. Carbohydrates provide energy to the body and is classified into three subtypes 1) ,2) and 3) . Lipids are hydrophobic or non-soluble in water because they are non-polar molecules. They are the building blocks of many hormones and are important constituent of the plasma membrane. Lipids include 4) , 5) , 6) and 7) . Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. 8) are monomers that make up protein. The functions of proteins are very diverse because there are 20 different chemically distinct amino acids that form long chains, and the amino acids can be in any order. For example, proteins can function as 9) or 10) . Nucleic acids are key macromolecules in the continuity of life. They carry the genetic blueprint of a cell and carry instructions for the functioning of the cell. The two main types of nucleic acids are 11) and 12) . 11 What I Can Do At this point, you are going to complete the table below by filling in the necessary information. Biomolecule Monomer Function Elements Structure Carbohydrates Lipids Proteins Nucleic Acids Assessment Directions: Read each item carefully. Use a separate sheet for your answers. Write only the letter of the correct answer for each question. 1. Fats and oils are known as . A. Peptides C. Polypeptides B. Polymers D. Triglyceride 2. What kind of polysaccharide is that which cannot be digested by human beings? A. Cellulose C. Starch B. Glycogen D. Sucrose 3. What is the function of carbohydrates? A. energy storage C. information storage B. digestion of foods D. building muscles and cells 4. These are made up of a hydrocarbon chain with an alcohol (–OH) group and a fatty acid. A. Fats and oils C. Steroids B. Phospholipids D. Waxes 12 5. The following statements about Cholesterol are true EXCEPT. A. It is a phospholipid B. It is the precursor of vitamins E and K. C. It is mainly synthesized in the liver and is the precursor of many hormones D. All of the above 6. These are macromolecules that are the key in the continuity of life. A. Carbohydrates C. Nucleic acids B. Lipids D. Proteins 7. These are the monomers of nucleic acids. A. DNA C. Polynucleotide B. Nucleotide D. RNA 8. Cellulose and starch are examples of . A. Disaccharide C. Oligosaccharide B. Monosaccharide D. Polysaccharide 9. How many amino acids are there? A. 20 B. 21 C. 22 D. 23 10. Which polysaccharide is stored in the liver and the muscles? A. Cellulose C. Glycogen B. Chitin D. Starches 11. Which is NOT a major source of protein? A. egg C. milk B. fish D. vegetable 12. The most abundant monosaccharide in nature is . A. glucose C. maltose B. lactose D. sucrose 13. Cholesterol is a/an . A. essential oil C. steroid B. saturated fat D. unsaturated fat 14. Which has the most lipids? A. banana C. cheese B. champorado D. olive oil 15. Which is a polysaccharide? A. glucose C. fructose B. glycogen D. lactose 13 Additional Activities Additional Activity 1: Concept Map on Biomolecules Directions: Complete the concept map below. Answer Key 1. D 2. A 3. C 4. D 5. A 6. A 7. C 8. B 9. A 10. C 11. C 12. B 13. D 14. A 15. C What I Know What’s In References Printed Materials: Acosta, H., L. Alvarez, D. Angeles, R. Arre, MP. Carmona, A. Garcia, A. Gatpo, et al. Science - Grade 10 Learner's Material. Pasig City, Philippines: Rex Bookstore, Inc. and Department of Education, 2015. Acosta, H., L. Alvarez, D. Angeles, R. Arre, MP. Carmona, A. Garcia, A. Gatpo, et al. Science - Grade 10 Science Teacher's Guide. Pasig City, Philippines: Rex Bookstore, Inc. and Department of Education, 2015. Electronic Sources: https://opentextbc.ca/biology/chapter/2-3-biological-molecules/ https://dlc.dcccd.edu/biology13/proteins#:~:text=Proteins%20are%20the%20most%20diverse,protect%20and%20maintain%20cell %20shape. https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Map%3A_Organic_Chemistry_(McMurr y)/27%3A_Biomolecules_-_Lipids https://courses.lumenlearning.com/microbiology/chapter/carbohydrates/#:~:text=Carbohydrates%2 C%20the%20most%20abundant%20biomolecules,chemically%20linked%20by%20glycosidic%20bond s. SCIENCE 10 LEARNING ACTIVITY SHEET MODULE 2: FOUR CATEGORIES OF BIOMOLECULES Name: Grade & Section: Date: Score: Most Essential Learning Competency: Recognize the major categories of biomolecules such as carbohydrates, lipids, proteins, and nucleic acids. Part 1. Analogy. Answer the following correctly. 1. Vegetable oil: lipid; table sugar: __________________ 2. Glucose: monosaccharide; starch: __________________ 3. __________________: fructose; dextrose: glucose 4. Grapes: __________________; malungay: polysaccharide 5. Galactose and glucose: __________________; glucose and fructose: Sucrose 6. Apple: __________________: milk: lactose 7. Monosaccharide: simple sugar: polysaccharide: __________________ 8. Table sugar: sucrose: __________________: fructose 9. __________________: high glucose; glucagon: low glucose 10. Glycogen: liver and muscles; __________________: adipose tissues Part 2. Fill in the Blanks. Complete the statements below. Choose your answers from the box below. 1. Lipids are _____________ in water. 2. Fats and oils are also known as _____________. 3. The presence of double bonds in the fatty acids lowers its _____________. 4. Fatty acids are insoluble in water but soluble in _____________ solvents. 5. Cholesterol is a kind of _____________. 6. _____________ is made from vegetable oils that are liquid at room temperature. 7. Garlic in dietary preparations lowers _____________. 8. _____________ is essential for cholesterol absorption. 9. Physical exercise_________ the serum cholesterol level. 10. Saturated fatty acids tend to have _____________ melting points than unsaturated fatty acids. Choices: nonpolar margarine steroid Cholesterol melting point bile Insoluble higher triglyceride Lowers Part 3. Matching Type: Match column A with column B. Write the letter only on the space provided. A B ____1. Good source of protein A. arginine ____2. They are synthesized by the body B. amino acid ____3. The biological catalysts C. beans ____4. Breaks down lactose into D. enzymes glucose and galactose E. lactase ____5. Building blocks of protein F. lysine ____6. Conditional amino acid G. nonessential amino acids ____7. Essential amino acid H. pentose ____8. Sequence of a chain of amino acid I. polymers ____9. Act as emulsifying agent J. primary protein structure ____10. Consist of monomers K. protein Part 4. Fill in the comparison table. Basis of Comparison DNA 1. Number of strands 2. Location in the cell 3. Type of sugar 4. Nitrogenous bases 5. Pairing of bases RNA