Urinalysis Training Checklist Clinical Laboratory- Estes Park Health System Overview Trainee Trainer initial/date initial/date Normal Operating Procedure Measures bilirubin, blood, ketones, leukocytes, nitrite, pH, protein, specific gravity, urobilinogen Power supply adaptor and AC power cord Test table with calibration bar Test table insert Paper roll- loading Printer- printer settings Interactive display Run screen Main screen Options and set up screen On/Off button Test compartment Barcode scanning Quality Control and Maintenance Two levels of quality control Quantimetrix level 1 Quantimetrix level 2 o New lots need to verify expected analyte range o Define QC values with new lots Performed with each new bottle of strips First of each month New lot of strips Recall of quality control results Calibration Automatic internal calibration Macroscopic quality control Qc material Check expiration Check analyte range Internal system check Operator name Enter new patient o QC1 or QC2 Drip qc material onto strip Blot strip Reagent strip in the channel with test pads facing up Strip automatically pulled into analyzer Trainee Trainer initial/date initial/date Urinalysis Training Checklist Clinical Laboratory- Estes Park Health Color and clarity- yellow & clear for QC Results print Rinse tray with H2O Record qc results Microscopic quality control Review urine sediments I and II charts Interlab consistency study annually Daily maintenance Remove insert Rinse both sides of table under running water Dry with kimwipe and replace insert Periodic maintenance Remove test table Clean test table with DI H2O, do not touch calibration bar Dry test table with kimwipe Replace test table insert Disinfecting test table and test table insert 5% bleach Wipe clean but not calibration bar Soak for 10 minutes Rinse with H2O Dry with kimwipe Cleaning white calibration bar Remove insert from table If dirty or discolored, wipe with cotton tipped swab and DI H2O Do not scratch Replace test table insert Cleaning outside of Clinitek Status+ Turn off analyzer Wipe outside with damp clamp and mild detergent Urinalysis Process Macroscopic Test urine within 30 minutes-2 hours If past 2 hours, store in fridge Operator name Patient identifier Strip test Urine color and clarity Results cross to LIS Clean tray after each use Microscopic Auto reflex for abnormal results on macroscopic Trainee Trainer initial/date initial/date Urinalysis Training Checklist Clinical Laboratory- Estes Park Health Formed elements: cells, crystals, casts, bacteria, yeast, artifact, parasites Test urine within 30 minutes – 2 hours If past two hours, store in fridge o Refrigeration may cause precipitation of amoprphous urates or phosphates o Return to room temp before testing Stat spin – pour off supernatant Microscopic review under microscope- 10 fields Elements viewed 10x- epithelial cells, casts, crystals, mucous, amorphous Elements viewed 40x- wbc, rbc, bacteria, yeast, parasites o Sperm- report present on male, do not report on female >18yrs, if female <18yrs have second tech confirm and notify physician Culture reflex Wet Prep Specimen collected by physician Tested within 20 minutes of collection Saline smear preparation onto slide Examine on 40x magnification Microscopic examination- trichomonas, clue cells KOH Specimen collected by physician Potassium hydroxide preparation onto slide Examine on 40x magnification Microscopic examination- yeast Epic documentation and resulting guidelines Trainee Trainer initial/date initial/date Macroscopic Results cross over to LIS Change method if necessary- Clinitek vs. Manual dipstick Microscopic Documented on form Enter into LIS Wet prep Report trichomonas and clue cells KOH Report yeast Trainee Signature: _______________________________________________ Date: __________ Trainer Signature: _______________________________________________ Date: __________ Urinalysis Training Checklist Clinical Laboratory- Estes Park Health Review Signature: _____________________________________________ Date:__________
