Prokaryotic Groups 1. Domain: Archaea 2. Domain: Bacteria - archaea and bacteria ~ “organisms” 1. Domain Bacteria a. Gram-Negative Bacteria i. Phylum Proteobacteria ➢ Most gram-negative, largest taxonomic grp ➢ Chemoheterotrophic ~ presumed it has arisen from common photosynthetic ancestor. ➢ phylogenetic relationship is based upon rRNA studies. 1. Class: Alphaprobacteria ➢ Most of probacteria ➢ Capable of growth within very low level of nutrients. ➢ Unusual morphology: prosthecae stalks or buds ➢ Agriculturally important capable of nitrogen fixation in symbiosis w/ plants, and human pathogens i. Pelagibacter (Pelagibacter ubique) ii. Azospirillum – soil bacterium growing in roots or tropical grasses iii. Acetobacteraceae – aerobic organism to convert ethanol to acetic acid (Acetobacter, Gluconobacter) 1. Ricketssia 2. Ehrlichia 3. Caulobacter and Hyphomicrobium 4. Rhizobium, Bradyrhizobium, and Agrobacterium 5. Bartonella 6. Brucella 7. Nitrobacter and Nitrosomonas 8. Wolbachia 2. Class: Betaproteobacteria ➢ uses nutrient substances that diffuse away from areas of anaerobic decomposition of organic matter (ammonia, methane, H gas) i. Acidthiobacillus ii. Spirillum iii. Sphaerotilus iv. Burkholderia v. Bordetalla vi. Neisseria vii. Zoogloea 3. Class: Gammoproteobacteria ➢ Largest subgroup ➢ Great variety of physiological types i. Order: Thiotrichales 1. Begglatoa (Beggiatoa) 2. Francisella ii. Order: Pseudomonadales 1. Pseudomonas 2. Azotobacter and Azomonas 3. Moraxella 4. Acinetobacter iii. Order: Legionellales 1. Legionella 2. Coxiella iv. Order: Vibronales v. Order: Enterbacteriales – anaerobic, gramnegative rod that are motile, and peritrichously flagellated; Eneterics – they inhabit the intestinal tracts of humans an dother animals; most are active fermenters of glucose and other carbohydrates 1. Escherichia 2. Salmonella 3. Shigella 4. Klebsiella 5. Serratia 6. Proteus 7. Yersinia 8. Erwinia 9. Enterobacter 10. Cronobacter vi. Order: Pasteurallales - non-motile, human and animal pathogens 1. Pasteurella 2. Haemophilus 4. Class: Deltaprotobacteria ➢ Distinctive; may include bacteriathat are predators on other bacteria ➢ Important contribution in sulfur cycle i. Bdellovibrio ii. Order: Desulfovibrionales – sulfur reducing; that oxidizes forms of sulfur such as sulfate iii. Order: Myxococcales 5. Class: Epsilonproteobacteria ➢ Slender gram-negative rod with helical or curved shape. ➢ 2 genera with both motile (flagella and microaerophilic) i. Campylobacter ii. Helicobacter Nonproteobacteria gram-negative bacteria 2. Cyanobacteria (The Oxygenic Photosynthetic Bacteria) - Blue green (cyan)/ blue green algae - Carry out photosynthesis and capable of fixing nitrogen gas from atmosphere (at hetercysts) - Uses gliding motility for locomotion. - simple diffusion, binary fission, colonial; reproduce by fragmentation of the filaments. 3. Phyla Chlorobi and Cloroflexi (Anoxygenic Photosynthetic Bacteria) - Chlorobi – green sulfur bacteria - Chloroflexi – green nonsulfur bacteria - Both produce no oxygen during photosynthesis - Photosynthetic gram-negative bacteria (purple sulfur bacteria – gammaproteobacterial) and purple nonsulfur bacteria (alpha and betaproteobacteria 4. Chlamydiae a. Chlamydia and Chlamydophila 5. Planctomycetes 6. Bacteriodetes a. Bacteroides b. Cytophaga 7. Fusobacteria a. Fusobacterium 8. Spirochaetes a. Treponema b. Borella c. Leptospira 9. Deinococcus-Thermus Gram-positive bacteria - Firmicutes (low G + C ratios) - Actinobacteria (high G + C ratios) 1. Firmicutes (Low G + C Gram Positive Bacteria) ➢ Important endospore-forming bacteria (e.g. genera Clostridium and Bacillus + Staphylococcus, Enterococcus, Streptococcus ++ Lactobacillus) i. Clostridiales 1. Clostridium 2. Epulopiscium ii. Bacillales – rods and cocci 1. Bacillus – rods and endospore 2. Staphylococcus – grape-like iii. Lactobacillales – lactic acid producing bacteria 1. Lactobacillus 2. Streptococcus a. Beta-hemolytic streptococci b. Non-beta-hemolytic streptococci 3. Enterococcus 4. Listeria 2. Tenericutes (Low G + C content) ➢ Wall-less bacteria “mycoplasmas” ➢ Highly pleomorphic due to lack of cell wall ➢ Produce filaments and resembles fungi 3. Actinobacteria (high G + C Gram-Positive Bacteria) ➢ Mycobacteriunm ➢ Corynebacterium ➢ Propionibacterium ➢ Garneralla ➢ Frankia ➢ Streptomyces ➢ Actinomyces ➢ Nocardia Domain Archaea - Lack peptidoglycan. - Shared many rRNA sequence. - Found in extreme environmental conditions Extremophiles (halophiles, thermophiles, and acidophiles) – pathogenic archaea