MLSPRAC02 Urine, Semen and Culture Desoacido, Dusaran, Lobrigo, Mandadero, Milla, Oida, Sy BSMT 1 - Lymphocytes Urine, Semen and Culture Urine liquid or semisolid solution of metabolic wastes and certain other, often toxic, substances that the excretory organs withdraw from the circulatory fluids and expel from the body. Urine has been used as an antiseptic for at least 2000 years primitive antiseptic for treating scorpion stings, snake bites, rabid dog bites, sores, burns, and many other conditions Urine, Semen and Culture Semen also called seminal fluid fluid that is emitted from the male reproductive tract and that contains sperm cells, which are capable of fertilizing the female’s eggs. Semen facials are actually a thing! Latest trend in skincare Spermine, an antioxidant and antiinflammatory agent can combat blemishes. Yet, no scientific evidences proves its benefits Urine, Semen and Culture Urine, Semen and Culture Procedures and Standards often ignored as the patient is doing the collection more time should be spent for best possible sample Patient Cooperation & Education sample collection accuracy proper and noncontaminated sample is collected and tested Sterilization samples must be collected in sterile containers for culture accuracy residues in unclean containers can alter results dramatically Urine Sample Collection Ways of Collecting Urine Samples Sterile Urine Bag mostly used with kids noninvasive and is often the clinician's 'kindest' choice, yet, it has the highest rate of contamination (up to 50%) Urethral Catheterization collected on a patient unable to void, babies, or bedridden patients. passing a sterile hollow tube through the urethra into the bladder Suprapubic Aspiration used when sample must be completely free of extraneous contaminations external introduction of a needle through the abdomen into bladder Clean-catch Midstream one of the least invasive procedures for a urine culture or urinalysis prevent bacteria from the skin of the penis or vagina from contaminating the urine specimen the procedure differs from men and women Urine, Semen and Culture Types of Urine Samples 1 First morning or 8-hour Sample 3 2 can be collected any time collected in 2 specific times 6 4 5 24-hour or Timed Sample collected at a specific time during a 24-hour period or after a specific activity Clean-catch Midstream Urine most common urine sample type to be collected Catheterized Urine collected when patient cannot voluntarily produce urine initial urine urine after drinking 200 mL of water and waits for 2 hours most concentrated sample samples collected by daysleeping patients Random Sample Double-voided Sample 7 Chain-of-custody Urine collected for preemployment physical or legal sample collection 8 Urine Culture Sample sample to be cultured within 1 hour of collection and refrigerated or used with preservative container Semen Sample Collection collected and tested to evaluate fertility and post-vasectomy procedures to determine number of and activity of sperm in the semen Collection at laboratory collected in a warm sterile container must not be collected in a condom because condoms frequently contain spermicidal agents. Collection at home must be kept warm and delivered to the laboratory within 1 hour. sample should be kept at 37°C. Factors affecting Semen Quality Urine, Semen and Culture Medication such as cimetidine (Tagamet), male and female hormones (testosterone, estrogen), sulfasalazine, nitrofurantoin, and some chemotherapy medication. Addictive Substances Caffeine, alcohol, cocaine, marijuana, and tobacco. Some drugs such as anabolic steroids can affect sperm production. Herbal Medicines St. John’s wort and high doses of echinacea. No lubricants including saliva do not use condom for collection as spermicidal agents will alter results Semen Sample Collection Ways of Collecting Semen Samples Masturbation ejaculate directly into a sterile container provided avoid touching the inside of the cup get first part of your ejaculation in the cup, as it is the most sperm-rich Artificial Vagina mostly used for ram semen comprised of a latex rubber cone attached to a 15 mL calibrated plastic centrifuge tube PESA percutaneous epididymal sperm aspiration sample of sperm cells is removed from the epididymis through a small needle attached to a syringe Urine, Semen and Culture Thank you! Desoacido, Stacy Dusaran, Darren Lobrigo, Liera Mandadero, Diona Milla, Zhemar Oida, Cheska Sy, Nicole
