Human enterovirus 71 outbreaks, Cambodia
From May, 2022 to July 2023
Abstract
Background:
Human enterovirus 71 caused a deadly disease outbreak in Cambodia in 2012, about 50 children with
cases of severe encephalitis died during this outbreak. From May 2022 to July 2023, an undiagnosed
illness with clinical manifestations of (skin rash, fever, mouth blisters) occurred in children admitted to
two pediatric hospitals (KTB, NPH) in Phnom Penh, Cambodia. This study aimed to analyze the
epidemiological characteristics and investigation of enterovirus 71 associated with hand, foot and
mouth disease.
Methods:
Clinical specimens (blood sample, nasopharyngeal and rectal swabs) were collected at Kantha Botha and
National Pediatric Hospitals, and extracted for total nucleic acid (DNA/RNA), pathogen determined by
RT-PCR multiplex for pan-EV and EV 71, CVA6, CVA16. The VP1 gene was amplified and sequenced for
the EV subtype.
Results:
A total of 173 clinical samples, EV71 was detected in 13 patients (23.21%), CVA6 in 6 patients (10.71%)
and other EVs in 7 patients (12.5%). The main symptoms were skin rash, fever, and mouth blisters, and
the laboratory results showed that enterovirus 71 was the main pathogen causing this outbreak.
Conclusion:
This manuscript further investigated EV71 infection, which was helpful to better identify and deal with
the epidemical characteristic and investigation the disease in the future.
Keywords: Hand foot and mouth disease outbreak, EV71, Coxsackievirus A6
56 patients: 17/03/2023
Human enterovirus 71 outbreaks, Cambodia
From May, 2022 to July 2023
Human enterovirus 71 caused a deadly disease outbreak in Cambodia in 2012, about 50 children with
cases of severe encephalitis died during this outbreak. From May 2022 to July 2023, an undiagnosed illness
with clinical manifestations of (skin rash, fever, mouth blisters) occurred in children admitted to two
pediatric hospitals (KTB, NPH) in Phnom Penh, Cambodia. This study aimed to analyze the epidemiological
characteristics and investigation of enterovirus 71 associated with hand, foot and mouth disease.
Methods: Clinical specimens (blood sample, nasopharyngeal and rectal swabs) were collected at Kantha
Botha and National Pediatric Hospitals, and extracted for total nucleic acid (DNA/RNA), pathogen
determined by RT-PCR multiplex for pan-EV and EV 71, CVA6, CVA16. The VP1 gene was amplified and
sequenced for the EV subtype. Results: A total of 173 clinical samples, EV71 was detected in 13 patients
(23.21%), CVA6 in 6 patients (10.71%) and other EVs in 7 patients (12.5%). The main symptoms were skin
rash, fever, and mouth blisters, and the laboratory results showed that enterovirus 71 was the main
pathogen causing this outbreak. Conclusion: This manuscript further investigated EV71 infection, which
was helpful to better identify and deal with the epidemical characteristic and investigation the disease in
the future.
I (introduction): Why the study was done?
M (methods): What was done or how?
R (results): What was found?
D (discussion): What was concluded, what did it mean?