SNSF Apreo Startup Checklis Login Badger (SNL tab, SEM-FIB Microscope Control Vent Chamber Check that stage is at Sample Exchange positio (X ~ 50, Y ~ 0, Z ~ 40 (up) Only use the pull-bar to move the door Load sample Finger-tight with the set screw only (if applicable) Close door; pump down Hold door closed until vacuum system is fully engaged – wait for the gate noise Take Nav Cam image Optional Move sample laterally to starting sample Do not raise the stage yet Wait for good vacuum Below 9.0 x 10-5 mbar Run sample cleaning Except if plasma cleaning will destroy the sample Fill CryoCleaner Optional Raise the stage Turn beam on; start imaging Check source centering and re-center crossover if necessary Verify sample height, link Focus at 5000X to 8000X n t ) ) Proceed with experiment