Dimension® Systems Advanced Hardware Diagnostics & Troubleshooting Local Area Training Workbook Siemens Healthineers Dimension Systems Advanced Hardware Diagnostics & Troubleshooting Local Area Training Workbook Dimension Systems T01008.095 Effective Date: 10/11/2021 © 2021 Siemens Healthineers Inc. All rights reserved. This manual, and the software described in this manual, are copyrighted. No part of this may be copied, reproduced, translated or reduced to any electronic medium or machinereadable form without the prior written consent of Siemens Healthineers. Dimension, EXL, Flex, LOCI, QCC PowerPak, and QuikLYTE are trademarks of Siemens Healthineers. All other trademarks are the property of their respective owners. ii Dimension Systems Table of Contents Dimension Systems 1: Welcome 2: System Check Troubleshooting 3: Electrical Cabinet 4: Photometer 5: Cuvette Manufacturing 6: Reagent Delivery System iii iv Dimension Systems Welcome 1 Welcome Welcome to Training Siemens Healthineers would like to welcome you to the Local Area training on the Dimension Systems. This course is designed to teach you the advanced skills needed to operate, maintain, and troubleshoot the Dimension Systems. Our staff welcomes the opportunity to present this training program to you. Dimension Systems 1-1 Welcome Training Center Safety Information While you are at the Training Center, please follow these safety practices: • Please wear your name badge at all times. • In the event of a fire alarm, Stop work immediately and leave the building through the nearest exit. Instructors will discuss the evacuation route. • Note the location of the fire extinguisher. • Use the eyewash located near the sink if you should happen to get anything in your eye(s).Report any injury to the instructor. • Carefully read the warnings, cautions, and notes in the guides and manuals. • Eating and drinking are not allowed in the instrument area of the classroom. • You must wear laboratory coats and gloves which are provided. Do not wear these garments outside the classroom. • Use safety glasses when operating the instrument or preparing samples. • Dispose of waste materials appropriately: • 1-2 o Biohazard Waste: Wastebaskets with red plastic liners o Paper Waste: Wastebaskets with clear plastic liners o Use sharps containers for the disposal of glass or sharp objects. Wash your hands before leaving the classroom and after removing your gloves. Dimension Systems Welcome Course Objectives Upon completion of this course, you will be able to: Dimension Systems • Describe the process and components involved in a System Check. • Demonstrate troubleshooting failed system checks. • Identify components of the Electrical cabinet, Photometer, Cuvette manufacturing and the Reagent Delivery System. • Identify error messages associated with the Electrical cabinet, Photometer, Cuvette manufacturing and the Reagent Delivery System. • Demonstrate troubleshooting the Electrical cabinet, Photometer, Cuvette manufacturing and the Reagent Delivery System. 1-3 Welcome Training Agenda Morning Afternoon Welcome System Check Troubleshooting 1-4 Lunch Cuvette Manufacturing Electrical Cabinet Reagent Delivery System Photometer Hands on Troubleshooting Dimension Systems Welcome Dimension® Systems Advanced Hardware Diagnostics & Troubleshooting Operator Training Course Validation Checklist The participant places a checkmark beside the competency when it is completed. When all competencies are checked, the instructor and participant sign and date below as record of completion. Topics Competencies System Check Troubleshooting Configure Supplies Alert and Auto Setup for system check Completed Describe the process and components involved in a System Check Demonstrate troubleshooting failed system checks Electrical Cabinet Identify electrical cabinet components Use the Power Diagnostics screen Perform the Instrument Power Shutdown and Startup Procedure Demonstrate replacement of a fuse Photometer Demonstrate replacement of the source lamp Demonstrate troubleshooting the photometer Cuvette Manufacturing Identify cuvette manufacturing components Demonstrate troubleshooting the cuvette manufacturing module Perform rethreading of cuvette film Reagent Delivery Identify reagent delivery components and error messages Demonstrate troubleshooting reagent delivery systems Demonstrate troubleshooting RMS associated error messages (EXL with LM only) Dimension Systems 1-5 Welcome Instructor: __________________________________________________ Student: __________________________________________________ Date: __________________________________________________ What was most helpful to you during this program? How can we improve this program to make it more meaningful to you? 1-6 Dimension Systems System Check Troubleshooting 2 System Check Troubleshooting Dimension Systems 2-1 System Check Troubleshooting System Check Troubleshooting Resources • Dimension EXL 200 / Dimension EXL with LM Operator’s Guide Objectives Upon completion of this exercise, you will be able to: 2- 2 • Configure Supplies Alert and Auto Setup for system check. • Describe the process and components involved in a System Check. • Demonstrate troubleshooting failed System Checks. Dimension Systems System Check Troubleshooting System Check Configuration Supplies Alert 1. Display the Reagent Cartridge Alert screen by pressing: Supplies. 2. F1: Config Alerts. 3. Cursor to the CHK reagent and enter 10. 4. Press F1: Store Alerts. Note: Entering 10 ensures that there will be enough tests present onboard the instrument to run automated system check as scheduled. Automated System Check 1. From the Operating menu, display the Daily Maintenance Routine screen, press: F4: System Prep 2. F8: Daily Maintenance. 3. To configure system check to run automatically press: 4. F8: Auto Setup. 5. Use the left and right arrow keys or the touch screen to toggle between fields. 6. To select the Start Day, use the Enter key to toggle through the days of the week. For this exercise set the start and END day to today. 7. Toggle to the Start Time field and enter the appropriate time. For this exercise enter 5 minutes from the current time using a 24 hour clock. 8. Toggle between OFF/ON to set Activate to ON. 9. Press F1: Save Changes. 10. Press Home to return to the main menu. Tips: 1. The automated system check will not run as programmed if the instrument remains on the Daily Maintenance or the Daily Maintenance Auto Run screens. 2. A series of warning screens appear prior to automated System Check starting. 3. The warnings appear in three time intervals: 5 minutes, 1 minute, and a final ten seconds prior to the scheduled start time of the routine. 4. Dimension Systems The instrument will also beep 5 times when the ten second warning is displayed. 2-3 System Check Troubleshooting System Check Process System Check Printout CHK Processing Steps Modules Checked None (blank cuvette) Photometer takes 2 readings, of blank cuvette, 43 seconds apart Photometer alignment Cuvette windows Source lamp Optical filters Photometer Wavelength 293 nm 340 nm 383 nm 405 nm 454 nm 510 nm 540 nm 577 nm 600 nm 700 nm Maximum 0.35 -0.21 -0.23 -0.23 -0.24 -0.23 -0.26 -0.23 -0.26 -0.21 Reagent #1 Results Mean: 395.67 SD: 0.40 1st 395.53 2nd 395.89 3rd 395.74 4th 396.11 5th 395.07 Reagent #2 Results Mean: 393.22 SD: 1.26 1st 394.25 2nd 394.51 3rd 393.29 4th 392.63 5th 391.41 Sampler Mean: 38.33 SD: 0.26 Results 1st 38.22 2nd 38.16 3rd 38.11 4th 38.39 5th 38.76 2- 4 Read 2 subtracted from Read 1 for the result Flex reagent cartridge R1 probe adds 60 µL of CHK and 420 µL of water to 5 different cuvettes R1 arm R1 probe R1 pump/syringe R1 alignments Flex reagent cartridge R2 probe adds 60 µL of CHK and 420 µL of water to 5 different cuvettes R2 arm R2 probe R2 pump/syringe R2 alignments Reaction Vessel R2 adds 50 µL CHK solution to 5 Reaction Vessel R1 adds 345 µL water to 5 cuvettes Sampler aliquots 10 µL CHK solution from Reaction Vessel followed by 45 µL to cuvettes R1 arm, probe R2 arm, probe Sample probe Sample pump panel/ syringe Dimension Systems System Check Troubleshooting System Check Printout HM Wash Results Mean: 38.53 SD: 0.09 1st 38.61 W1 2nd 38.47 W1 3rd 38.54 W2 4th 38.62 W2 5th 38.42 W2 Sample Probe Cleaner CHK Flex reagent cartridge Processing Steps 1. R2 adds water to 5 reaction vessels. 2. Wash probes 1 and 2 aspirate and dispense fluids from Reaction Vessel. 3. R1 adds water to cuvettes. 4. R2 adds CHK and water to Reaction Vessel. 5. Sampler aliquots 5 replicates of CHK from reaction vessels to 5 cuvettes. Modules Checked R1 system R2 system Sampler system HM wash probe 1 HM wash probe 2 HM wash pump tubing and cassette Cleaners are delivered to cuvette. Presence checked photometrically. Reagent and sample probe cleaner delivery systems None (blank reaction vessel) 1. Sampler appears to deliver to reaction vessel but no fluid is added. 2. LOCI arm transfers an empty vessel to the read chamber. 3. LOCI reader performs a standard assay read. 4. LOCI arm transfer 4 more vessels for a total of 5 reads. LOCI module Flex reagent cartridge 1. R3 probe adds CHK from well 3 or 6 and water to well 7 or 8 of CHK reagent cartridge. 2. R1 probe aliquots 5 replicates of CHK from diluted well to 5 cuvettes. R3 arm R3 pump/syringe R1 arm R1 pump/syringe None Reagent Probe Cleaner LOCI module Results <0.144 1st 0.025 2nd 0.020 3rd 0.030 4th 0.023 5th 0.033 RMS Results Mean: 398.32 SD: 0.68 1st 398.41 2nd 397.73 3rd 399.21 4th 398.70 5th 397.56 Dimension Systems 2-5 System Check Troubleshooting System Check Troubleshooting Failed Module Resolution Photometer Perform Photometer Alignment Clean Cuvette Windows Check Cuvette Film Replace Source Lamp Replace Optical Filter Reagent R1, R2 or R3 (RMS) Confirm Correct Carton Value Replace / Align Reagent Probe Investigate Reagent Pump Panel for leaks or crimped tubing Perform Reagent Probe Ultrasonics test Sampler Confirm Correct Carton Value R1 or R2 precision or accuracy (resolve R1 or R2 issue first) Replace / Align Sample Probe Investigate Sample Pump Panel Perform Sample Probe Ultrasonics test HM Confirm Correct Carton Value R1, R2 or Sampler precision or accuracy (resolve failed Probe issue first) Replace/ align W1 or W2 probes Replace pump cassette or tubing Sample or Reagent Probe Cleaners Check Probe Cleaner solution or bottle Check Tubing or connectors for leaks Replace Pump Cassette LOCI Reset Statistics Replace/align Transfer arm vacuum cup Replace LOCI insert and rubber seal 2- 6 Dimension Systems System Check Troubleshooting * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * ** * Dimension Training * 10:25 Nov 15 20XX * SYSTEM CHECK * Entered: 10:15 Nov 15 20XX * Status: FAIL * CHK Flex Lot # DR0095 * PHOTOMETER Wavelength 293nm 340nm 383nm 405nm 452nm 510nm 540nm 577nm 600nm 700nm REAGENT #1 Mean: 393.87 SD: 0.87 REAGENT #2 Mean: 393.42 SD: 0.97 Maximum 4.90 3.02 2.00 1.57 0.64 0.55 0.58 0.55 0.50 0.41 1st 2nd 3rd 4th 5th Results 394.08 392.97 393.22 393.90 395.19 1st 2nd 3rd 4th 5th Results 391.70 393.90 393.70 393.91 393.90 SAMPLER Mean: 39.08 SD: 0.11 Results 1st 39.05 2nd 38.96 3rd 38.99 4th 39.20 5th 39.18 * * * * * * * * * * * * * * * * * HM WASH Results Mean: 39.05 1st 39.15 SD: 0.18 2nd 39.02 3rd 38.99 4th 39.29 5th 38.80 Sample Probe Cleaner PASS Reagent Probe Cleaner PASS * * W1 W1 W2 W2 W2 * * * * * * * * * * * * * * * * * * * LOCI Results 1st 0.025 2nd 0.020 3rd 0.030 4th 0.023 5th 0.033 * * * * * * * * * * * * * * * * * * * Dimension Systems * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * POSSIBLE CAUSES Scenario 1 RESOLUTION 2-7 System Check Troubleshooting POSSIBLE CAUSES * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * 2- 8 * * * * * * * * * * * * * * * * * * * * * * Dimension Training 10:25 Nov 15 20XX SYSTEM CHECK Entered: 10:15 Nov 15 20XX Status: FAIL CHK Flex Lot # DR0095 PHOTOMETER Wavelength 293nm 340nm 383nm 405nm 452nm 510nm 540nm 577nm 600nm 700nm * * * * * * Maximum 0.84 0.61 0.53 0.53 0.64 0.55 0.58 0.55 0.50 0.41 REAGENT #1 Mean: SD: 393.87 0.87 1st 2nd 3rd 4th 5th Results 394.08 392.97 393.22 393.90 395.19 REAGENT #2 Mean: SD: 391.04 7.26 1st 2nd 3rd 4th 5th Results 395.76 394.70 393.68 378.19 392.86 SAMPLER Mean: SD: * * * Results 1st 39.05 2nd 38.96 3rd 38.99 4th 39.20 5th 39.18 * * * * * * * * * * * * * * * * * * * * * HM WASH Results Mean: 39.05 1st 39.15 W1 SD: 0.18 2nd 39.02 W1 3rd 38.99 W2 4th 39.29 W2 5th 38.80 W2 39.08 0.11 Sample Probe Cleaner PASS Reagent Probe Cleaner PASS * * * * * * * * * * * * * * * * * * * * * LOCI Results 1st 0.025 2nd 0.020 3rd 0.030 4th 0.023 5th 0.033 * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Scenario 2 RESOLUTION Dimension Systems System Check Troubleshooting POSSIBLE CAUSES * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * ** * Dimension Training 10:25 Nov 15 20XX SYSTEM CHECK Entered: 10:15 Nov 15 20XX Status: FAIL CHK Flex Lot # DR0095 PHOTOMETER Wavelength 293nm 340nm 383nm 405nm 452nm 510nm 540nm 577nm 600nm 700nm REAGENT #1 Mean: 393.87 SD: 0.87 REAGENT #2 Mean: 393.42 SD: 0.97 Maximum 0.84 0.61 0.53 0.53 0.64 0.55 0.58 0.55 0.50 0.41 1st 2nd 3rd 4th 5th Results 394.08 392.97 393.22 393.90 395.19 1st 2nd 3rd 4th 5th Results 391.70 393.90 393.70 393.91 393.90 SAMPLER Mean: 39.08 SD: 0.11 Results 1st 39.05 2nd 38.96 3rd 38.99 4th 39.20 5th 39.18 * * * * * * * * * * * * * * * * * * HM WASH Results Mean: 39.05 1st 39.15 W1 SD: 0.18 2nd 39.02 W1 3rd 38.99 W2 4th 39.29 W2 5th 38.80 W2 Sample Probe Cleaner PASS Reagent Probe Cleaner PASS * * * * * * * * * * * * * * * * * * LOCI Results 1st 1.025 2nd 1.030 3rd 1.028 4th 1.032 5th 1.040 * * * * * * * * * * * * * * * * * * Dimension Systems * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Scenario 3 RESOLUTION 2-9 System Check Troubleshooting POSSIBLE CAUSES * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * 2- 10 * * * * * * * * * * * * * * * * * * * * * * Dimension Training 10:25 Nov 15 20XX SYSTEM CHECK Entered: 10:15 Nov 15 20XX Status: ***** CHK Flex Lot # DR0095 PHOTOMETER Wavelength 293nm 340nm 383nm 405nm 452nm 510nm 540nm 577nm 600nm 700nm REAGENT #1 Mean: *** SD: *** REAGENT #2 Mean: *** SD: *** Maximum *** *** *** *** *** *** *** *** *** *** 1st 2nd 3rd 4th 5th Results 394.08 392.97 393.22 393.90 395.19 1st 2nd 3rd 4th 5th Results 391.70 393.90 393.70 393.91 393.90 SAMPLER Mean: *** SD: *** 1st 2nd 3rd 4th 5th * * * * * * * * * * * * * * * HM WASH Mean: *** 1st SD: *** 2nd 3rd 4th 5th Sample Probe Cleaner PASS Reagent Probe Cleaner PASS Results *** *** 38.99 39.20 39.18 * * * * * * Results 39.15 W1 39.02 W1 38.99 W2 39.29 W2 38.80 W2 * * * * * * * * * * * * * * * * * * * * * LOCI Results 1st 0.025 2nd 0.020 3rd 0.030 4th 0.023 5th 0.033 * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Scenario 4 RESOLUTION Dimension Systems System Check Troubleshooting POSSIBLE CAUSES * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Dimension Training * 10:25 Nov 15 20XX * SYSTEM CHECK * Entered: 10:15 Nov 15 20XX * Status: FAIL * CHK Flex Lot # DR0095 * PHOTOMETER Wavelength 293nm 340nm 383nm 405nm 452nm 510nm 540nm 577nm 600nm 700nm REAGENT #1 Mean: 393.87 SD: 0.87 REAGENT #2 Mean: 393.42 SD: 0.97 SAMPLER Mean: SD: Maximum 0.84 0.61 0.53 0.53 0.64 0.55 0.58 0.55 0.50 0.41 1st 2nd 3rd 4th 5th Results 394.08 392.97 393.22 393.90 395.19 1st 2nd 3rd 4th 5th Results 391.70 393.90 393.70 393.91 393.90 Results 1st 0.45 2nd 0.87 3rd 0.63 4th 0.59 5th 0.64 * * * * * * * * * * * * * * * * * * * * * HM WASH Results Mean: 1.67 1st 1.24 W1 SD: 0.3 2nd 1.54 W1 3rd 1.81 W2 4th 2.05 W2 5th 1.69 W2 0.64 0.15 Sample Probe Cleaner PASS Reagent Probe Cleaner PASS * * * * * * * * * * * * * * * * * * * * * LOCI Results 1st 0.025 2nd 0.020 3rd 0.030 4th 0.023 5th 0.033 * * * * * * * * * * * * * * * * * * * * * Dimension Systems * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Scenario 5 RESOLUTION 2-11 System Check Troubleshooting * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * 2- 12 * * * * * * * * * * * * * * * * * * * * * * Dimension Training 10:25 Nov 15 20XX SYSTEM CHECK Entered: 10:15 Nov 15 20XX Status: FAIL CHK Flex Lot # DR0095 PHOTOMETER Wavelength 293nm 340nm 383nm 405nm 452nm 510nm 540nm 577nm 600nm 700nm REAGENT #1 Mean: 393.87 SD: 0.87 REAGENT #2 Mean: 393.42 SD: 0.97 SAMPLER Mean: SD: 39.08 0.11 Maximum 0.84 0.61 0.53 0.53 0.64 0.55 0.58 0.55 0.50 0.41 1st 2nd 3rd 4th 5th Results 394.08 392.97 393.22 393.90 395.19 1st 2nd 3rd 4th 5th Results 391.70 393.90 393.70 393.91 393.90 1st 2nd 3rd 4th 5th * * * * * * * * * * * * * * * * * * HM WASH Mean: 24.85 1st SD: 13.58 2nd 3rd 4th 5th Results 39.05 38.96 38.99 39.20 39.18 * * * Results 38.98 39.02 14.72 9.85 21.67 Sample Probe Cleaner PASS Reagent Probe Cleaner PASS * * * * * * * * * * * * * * * * * * * * * LOCI Results 1st 0.025 2nd 0.020 3rd 0.030 4th 0.023 5th 0.033 * * * * * * * * * * * * * * * * * * * * * W1 W1 W2 W2 W2 POSSIBLE CAUSES * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Scenario 6 RESOLUTION Dimension Systems Electrical Cabinet 3 Electrical Cabinet Dimension Systems 3-1 Electrical Cabinet Electrical Cabinet Resources • Dimension EXL 200 / Dimension EXL with LM Operator’s Guide Objectives Upon completion of this exercise, you will be able to: 3-2 • Identify Electrical Cabinet components. • Use the Power Diagnostics screen. • Perform the Instrument Power Shutdown and Startup Procedure. • Demonstrate replacement of a fuse. Dimension Systems Electrical Cabinet Electrical Cabinet Component Reminders/Tips Fuse Board A 5V, ±15V fuses (red LEDs) and +24V fuses (green LEDS) Fuse Board B +24V fuses (all green LEDs) Notes CR- Current Resistor PC Boards Photometer Cuvette Empty or QCC Motor Control Board 1 Motor Control Board 2 Motor Control Board 3 Auxiliary Ultrasonics Note: The digital display on the board is 1 to indicate all is OK. Any other number or letter suggests a problem. Always use the ground wrist strap when working with PC boards. Static electricity transferred to a board can damage it. To Confirm a Blown Fuse From the Operating Menu: 1. Select F7: DIAGNOSTICS. 2. Select F1: ELECTRO/MECH. 3. Select F7: POWER SYSTEM. Note: Before you replace any fuse, the instrument must be turned off via the Controlled Power Shutdown procedure. Dimension Systems 3-3 Electrical Cabinet Performing a Controlled Power Shutdown From the Operating Menu 1. Select the sign out icon. 2. Select Shutdown 3. When prompted: “If you really want to shut down the application, type y” select Yes to confirm. 4. At the console menu, select option 4. Shutdown. 5. At the Shutdown Menu, select option 2. Shutdown. 6. When the message “Do you really want to shut down the computer? Select OK. 7. When the screen turns black, turn off the instrument power switch. Replacing a Fuse Using a flat head screwdriver remove the fuse holder from the correct fuse board. Replace the damaged fuse with a new one of the same amperage/voltage found in the Dimension spare parts kit. Restoring Power after a Controlled Shutdown 1. Push the instrument power switch to its up position. 2. At the console menu, select option 1 to start the Dimension System. 3. Sign out of GuestUser by selecting the sign out icon. 4. Select Change Operator. 5. Sign in using your Operator ID and password. 6. When the Operating Menu appears, run a System Check and QC to ensure that all instrument systems are operating properly. Toggling between the Gray Screen and Black Screen Select ALT 1: Gray screen Select ALT 2: Black screen 3-4 Dimension Systems Photometer 4 Photometer Dimension Systems 4-1 Photometer Photometer Resources • Dimension EXL 200 / Dimension EXL with LM Operator’s Guide • Dimension EXL Systems Quick Reference Guide Objectives Upon completion of this exercise, you will be able to: 4-2 • Demonstrate replacement of the source lamp. • Demonstrate troubleshooting the photometer. Dimension Systems Photometer Photometric Measurement System Photometer Diagnostics Screen F7: Diagnostics > F1: Electro/Mech > F5: Photometer Acceptable Limits: Lamp voltage: >6.0 and < 8.0 volts When troubleshooting with technical support, it may be recommended to replace lamp if voltage reading is below 6.8 Measure frequency: >30,000 and <135,000 Hz (lamp on high) at blank cuvette for all filters except 293 Dimension Systems 4-3 Photometer Photometer Module Troubleshooting Error Message Steps to Resolve Notes Photometer Source Lamp Problem The computer did not receive a signal that the source lamp was on. (278 ) Photometer Source Lamp Off Detected Check if LED CR3B on the photometer board is lit and check Photometer Diagnostic screen. If CR3B is out and/or lamp amps <0.1 Amp and lamp voltage >20 Volts: Lamp problem - Perform controlled power shutdown and lower the thermal chamber. - Check that the source lamp connector (P/J 72B) is connected - Replace the source lamp, perform Photometer alignments and all steps in Replacing Source Lamp procedure If lamp voltage is 0, it is not the lamp: - Check the source lamp fuse 24 1D If error message reappears, call CCC-TS. Other probable causes could be a photometer board problem or bad fuse 24 1C. Photometer Diagnostics: Photometer gross (276) and fine (277) positioning error 1. If the photometer does not move through its The photometer did not move complete initialization cycle: Check for the proper distance as verified interference (film, wiring, and tubing). The by the encoder. If the most typical cause of this is the photometer disagreement between the hitting bunched up film or the thermal motor and the encoder is 3 to 7 chamber. Perform controlled power shut steps, it is considered a fine down, lower the thermal chamber, ensure all error. If 8 or more, a gross error. cables and tubing are out of the way, and reseat it. 2. If the photometer moves without stopping: - Cycle the photometer - If the Home field does not change to On and then Off during this cycle or if the photometer gets to -11 and makes a loud noise, the sensor has failed. - Change the photometer home sensor. After restoring power perform photometer alignments, System Check and Daily QC 3. If the photometer does not move at all: The fuse may have opened. Check fuse 24 1C on Fuse board A; if not lit, replace the fuse. F7:DIAGNOSTICS F1:ELECTRO/MECH F5:PHOTOMETER Photometer Mispositioned 4-4 F7:DIAGNOSTICS F1:ELECTRO/MECH F5:PHOTOMETER F8:CYCLE Dimension Systems Photometer Replace and Align Photometer Source Lamp Use the Dimension EXL Systems Resource Guide. List the required steps after replacing the source lamp and restoring power to the system. 1. 2. 3. 4. 5. Dimension Systems 4-5 Cuvette Manufacturing 5 Cuvette Manufacturing Dimension Systems 5-1 Cuvette Manufacturing Cuvette Manufacturing Resources • Dimension EXL 200 / Dimension EXL with LM Operator’s Guide Objectives Upon completion of this exercise, you will be able to: 5-2 • Identify cuvette manufacturing components. • Demonstrate troubleshooting the cuvette manufacturing module. • Perform rethreading of cuvette film. Dimension Systems Cuvette Manufacturing Cuvette Manufacturing on the Dimension System Dimension Systems 5-3 Cuvette Manufacturing Digital Gauge Panel on the Dimension System Pressure Gauge* 20 psi ±2.0 (min. 15 psi) Flow meter* (4.0 lpm to 9.0 lpm) Vacuum Gauge** (>3.0 Hg) *Digital pressure gauge has pressure switch built in. **Vacuum gauge reading for instrument waste system 5-4 Dimension Systems Cuvette Manufacturing Error Message Steps to Resolve Notes Bad Cuvette (261) Heat Torch Timed Out - Check that the solenoid arms are locked in place. - Check the heat torch connector P/J 13K is connected - If CR3B is not on (at all), check fuse 24 2B on fuse board “A”. - Check that LED CR3B on cuvette board is pulsing in standby, but comes full on at the start of a cuvette formation, then begins to blink on and off. Press ALT/M F7: DIAGNOSTICS F1: ELECTRO/MECH F4: CUVETTE Failed heat torch. -Using the Operator’s Guide replace heat torch Bad Cuvette (260) Cuvette Not Sealed - Check that cuvette manufacturing U-seal solenoid arm is locked in place - U-seal connector is plugged in - Clean and lubricate the U seal solenoid Press ALT/M F7: DIAGNOSTICS F1: ELECTRO/MECH F4: CUVETTE Failed U-seal - Using the Operator’s Guide replace U-seal Bad Cuvette (259) Cuvette Air Pressure too low - Check cuvette diaphragm to ensure it is seated properly and it doesn’t have a hole. Replace if necessary - Check that nozzle solenoid bracket is positioned properly behind plunger Press ALT/M F7: DIAGNOSTICS F1: ELECTRO/MECH F4: CUVETTE - Check to ensure the rubber tip is still positioned on the plunger - Clean and lubricate the nozzle solenoid - In Cuvette diagnostics, cycle cuvettes and Monitor air flow and pressure: Air Flow 4.0 – 9.0 lpm Air Pressure 20 ± 2 psi Dimension Systems 5-5 Cuvette Manufacturing Bad Cuvettes Repeatedly Formed (508) Three bad cuvettes sequentially formed If the system generates three sequential bad cuvette error messages, it will generate this error Check the Error Log for the first “Bad Cuvette” error that appeared. Troubleshoot the minor error associated with the first “Bad Cuvette” error. Cuvette Wheel Can Not Find Position 061, 062 Cuvette wheel sensor problem or wheel did not move 257 Cuvette wheel index error - Capstan lever not locked into place - Look for obstructions: anything inhibiting the movement of the cuvette wheel, for example cuvette film jam in idler (nip) roller latch area, alignment gauges or thermometer adapter left in cuvette hole, cuvette window not seated properly - Clear the obstruction. - Film slack, tension film or cycle cuvettes. - Failed cuvette ring position sensor. - Using Operator’s Guide, replace sensor. F7: DIAGNOSTICS F6: ERROR LOG F5: MORE INFO Press ALT/M F7: DIAGNOSTICS F1: ELECTRO/MECH F4: CUVETTE If cuvette wheel does not move at all, - Check fuse LED indicator on (Fuse Board A 242A). Cuvette Failed Photometric QC Check 5-6 (262) Bad cuvette QC - Check to ensure film has not run out - Dirty windows - Source lamp weak (old) Use Operator’s Guide to replace source lamp Dimension Systems Cuvette Manufacturing Rethread Cuvette Film Before performing each step, read the entire step to be aware of any requirements, warnings, or cautions. Only trained operators should perform this procedure. 1. With the instrument in Standby, raise the reagent lid. 2. From the Operating Menu, press F7: DIAGNOSTICS F1: ELECTRO/MECH F4: CUVETTE F4: U-SEAL SOLENOID twice 3. Raise the barcode reader to the left. 4. Loosen the alignment bar thumbscrew and lift the alignment bar up and out of the way. WARNING: When doing the next step, do not touch the u-seal solenoid. It is hot 5. Pull the curved locking bar on the u-seal solenoid assembly to the right. Move the assembly to the left. 6. Pull the cuvette formation assembly away from the cuvette wheel. A rubber band can be used to hold the cuvette formation assembly back and out of the way. 7. Locate the Top Seal assembly. Disconnect the J2 black cable, which is the top connector from the control board. This will remove the heat from the top seal element. The red and yellow indicator lights will turn off when element temperature is cool. 8. Open the cabinet door. Remove the cuvette waste container. Dimension Systems 5-7 Cuvette Manufacturing 9. Unhook the latch of the capstan lever located directly above the cuvette waste container area and remove any film left around the cuvette wheel. 10. Pull approximately 4 feet of the right side film strip from the film cartridge. This allows you to rethread completely around the cuvette wheel. This helps with keeping the correct tension on the film while threading. 11. Feed the right film strip from the bottom of the film guide into the right film guide until you see the end come out the left side of the self-feeding guide at a 45-degree angle. 12. Attach a 2-inch piece of tape to the end of the right film strip. Line up the film with the cuvette wheel. Press firmly on the tape to secure the right film strip to the wheel. 13. Advance the cuvette wheel in a clockwise direction, by grasping the wheel left of the capstan motor, until you see the taped film appear to the left of the capstan. There is only about 1 inch of access left of the capstan motor. Once you see the film appear to the left of the capstan, stop moving the wheel and hold it stationary. Do not move the cuvette wheel counterclockwise or you will lose tension. 14. Keep tension on the film as you peel the taped strip from the wheel. Use a small screw drive to help detach the tape. 5-8 Dimension Systems Cuvette Manufacturing 15. While pulling the film, 10 inches, towards yourself (not down), wrap the film around the capstan and hook the latch of the capstan lever into place. 16. Insert the left film strip into the left film self-feeding guide until you see it come out the left side of the guide, pass 3 cuvette positions, pass where the u-seal will seal. 17. Place all components back into place. 18. Confirm that the position of the left film is far enough for the u-seal to seal the left film to the right film. 19. Reconnect the J2 black cable to control board, confirm all 3 lights are ON, indicating heat has been restored to the top seal element. 20. On the Cuvette Diagnostic screen, type 50 in the Cycle Count box and press the Enter key. To advance both sides of film completely off of the cuvette wheel, type in 110 cuvettes (15min). 21. Press F1: Cycle. The instrument will make cuvettes. Watch to confirm cuvettes are advancing and no error messages occur. Lower the reagent lid, close cabinet door. Dimension Systems 5-9 Reagent Delivery Systems 6 Reagent Delivery Systems Dimension Systems 6-1 Reagent Delivery Systems Reagent Delivery Systems Resources • Dimension EXL 200 / Dimension EXL with LM Operator’s Guide Objectives Upon completion of this exercise, you will be able to: 6-2 • Identify reagent delivery system components. • Demonstrate troubleshooting the reagent delivery systems. • Demonstrate troubleshooting RMS associated error messages (EXL with LM only). Dimension Systems Reagent Delivery Systems Reagent Module Movements and Sensors Module Movement Sensor Name Probe Up/Down R1, R2 or R3 vertical home sensor Carriage In/Out R1, R2 or R3 radial home sensor Arm (R2 only) CW/CCW R2 angular home sensor Error Message Steps to Resolve Notes Cannot Find Home May be caused by: cfh=cannot find home - Drains overflowing onto sensor or tubing leaking onto sensor To test motor/sensor: soh=stuck on home - Defective sensor - Loose or unplugged connectors or defective cables or connectors - Blown fuse - Defective motor or motor control board To resolve: Press Reset to initialize the module and observe movement. If it MOVES through a complete initialization cycle without stopping: - Locate the sensor LED on the appropriate board using fuse/PC board label on instrument door. - If possible, move the module into and out of its home position, checking for the LED to turn on and off. - If it does not turn on and off, clean or replace the sensor. If it DOES NOT MOVE through its complete initialization cycle: - Suspect interference, i.e. tubing or cabling restricting movement If it does not move at all: - Check fuse boards for blown fuses (fuse LED off), or verify in power diagnostics screen. F7: DIAGNOSTICS F1: ELECTRO/MECH F1: REAGENT F1: REAGENT 1 F2: REAGENT 2 F2: TEST MTR ONLY (View HOME column for Pass or Fail) OR To Cycle Arm: F8: ADVANCED DIAG F7: CYCLE ARM View “Sensors” status field. Verify status changes state (ON/OFF). - If fuse LED is off or DC voltage is in red, use the Operator’s Guide to replace fuse with the same type. If failure persists, suspect motor or motor control board Lost Steps - Interference (i.e., reagent tray lid latch, HM tubing) Reagent Diagnostics mfh=moving from home - Use F7: Cycle Arm function in Reagent Diagnostics to visually check for mechanical interference. mth=moving to home - Clean with alcohol then lubricate silver guide rod, and brass splined shaft with TufOil. DO NOT lubricate “top” silver splined shaft. F7: DIAGNOSTICS F1: ELECTRO/MECH F1: REAGENT F8: ADVANCED DIAG F7: CYCLE ARM - Check alignments Dimension Systems 6-3 Reagent Delivery Systems R1, R2, R3 Metering or Flush Pump Lost Steps - Use Reagent Diagnostics or Pump Prime to cycle pumps. Reagent Diagnostics - Investigate syringe in question for breaks, leaks, etc.; replace if necessary F7: DIAGNOSTICS F1: ELECTRO/MECH F1: REAGENT F8: ADVANCED DIAG F7: CYCLE PUMP - Clean/lubricate silver guide rod with TufOil and lead screw with grease lubricant. - Check syringe gap setting specs (.005 - .010 inches or printer paper folded to four equal thicknesses) using the Operator’s Guide. R1, R2, or R3 Metering or Flush Pump Cannot Find Home OR - Replace the probe or tubing Pump Prime: F4: SYSTEM PREP F7: PUMP PRIME - Verify syringe home sensor operation by viewing sensor status in Reagent Diagnostics. View Sensors status field Meter: ON or Flush: ON status. Verify status changes - Check syringe gap setting specs (0.005 – 0.010 inches or printer paper folded to four equal thicknesses) using the Operator’s Guide. - Replace pump optical sensor using the Operator’s Guide. R1, R2, or R3 - Check tightness of probe tip. Ultrasonics Unable to Mix - Check R1/R2/R3 probe alignments. - Confirm probe body is aligned (centered). - Use Ultrasonics Diagnostic screen to verify lock status is > 98 100% Ultrasonics Diagnostics: F7: DIAGNOSTICS F1: ELECTRO/MECH F8: ULTRASONICS F1: SELECT PROBE (If probe selected is Sample, press F5: Fill Sample Cup first) F2: ENABLE ON/OFF Reagent Preparation Error (381) Process error during preparation (382) Prepared reagent failed quality assurance - Never press reset. Press Alt/M or go to Error Log. Find Reagent Preparation error and look at the Time column to determine if previous message listed in log is related. If time is the same, troubleshoot the previous message, not the Reagent Preparation error. Error Log: F5: PROCESS CTRL F6: ERROR LOG OR F7: DIAGNOSTICS F6: ERROR LOG 6-4 Dimension Systems Reagent Delivery Systems Aligned Probe Body Misaligned Probe Body Dimension Systems 6-5 Reagent Delivery Systems Reagent Management System (RMS) Reagent Management System Overview The RMS functions to allow the Dimension system to process samples without interruption by: 6-6 • Providing the capacity for 44 additional refrigerated Flex® reagent cartridges. • Providing the capacity to load 18 Flex reagent cartridges at one time • Hydrating Flex reagent cartridges. • Automatically transferring Flex reagent cartridges to the Dimension system. • Automatically removing any empty or expired Flex reagent cartridges. Dimension Systems Reagent Delivery Systems Major Components of the RMS Module 1. Load tray 2. Elevator 3. Turntable 4. RMS reagent tray 5. Hydration station 6. R3 reagent probe 7. Bar code reader 8. Laser bar code reader 9. Hydration Station Sensor Assembly These four components are located inside the RMS cabinet. Dimension Systems • Card cage boards • R3 reagent pump/syringe • Main power switch • Waste container 6-7 Reagent Delivery Systems RMS Error Messages RMS Shuttle Lost Steps - Clean shuttle rails F7: DIAGNOSTICS - Perform RMS shuttle and turntable alignments F1: ELECTRO/MECH - Cycle the shuttle in diagnostics F1: REAGENT - Belt cracked/frayed. Call CCC-TS. F7: RMS F5: FLEX TURNTABLE F2: TEST MTR ONLY RMS Hydration Station Sensor Error If reagent cartridge is located in hydration station: Fix Inventory: - Use the verify slot on the Fix Inventory screen to remove (slot 88) F4: SYSTEM PREP - Check for bent R3 probe F6: FIX INVENTORY If NO reagent cartridge is located in hydration station: Cartridge Position Sensor. - Check LED CR2B on RMS Motor Control board – should be OFF. Use finger to push in metal wire in hydration station to raise flag gently, LED should turn ON and sensor will change status RMS barcode error F1: INVENTORY RMS Diagnostics F7: DIAGNOSTICS F1: ELECTRO/MECH F1: REAGENT F7: RMS - Replace the RMS hydration station sensor using the RMS Operator’s Guide F1: REAGENT R3 Barcode mismatches occur when the laser barcode reader either cannot read or reads the wrong barcode. The cartridge will be put in the reagent hold inventory area for further investigation. Fix Inventory: - Enter reagent hold area of inventory - If the system cannot verify the reagent, remove from the system. - Inspect reagent cartridge for mismatch or defective label. - Clean laser barcode reader Cartridge present No→Yes F4: SYSTEM PREP F1: INVENTORY F1: SHOW HOLD F2: SHOW MISMATCH Select Method F3: VERIFY SLOT F2: REMOVE DATA - Align laser barcode reader 6-8 Dimension Systems