2.7 DNA Replication, Transcription and Translation DNA Replication Explain how DNA replication is semi-conservative ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... Outline how the results of the Meselson-Stahl experiment supported semi-conservative DNA replication ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... Describe the role of the following enzymes in DNA replication Helicase: ………………………………………………………………………………………………………………………………………… ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... DNA Polymerase: ……………………………………………………………………………………………………………………………. ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... Explain the significance of complementary base pairing in the conservation of base sequence ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... State when and where DNA replication happens in a typical eukaryotic cell ………………………………………………………………………………………………………………………………………………….......... Describe the purpose and process of the polymerase chain reaction (PCR) ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… Transcription Define transcription ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... Distinguish between sense and antisense strands ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... Outline the role of RNA polymerase in the process of transcription ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... Convert the following DNA sequence into an RNA transcript T A C A A A T T C G T A C T G C A C T C C G G A A C A A C T ………………………………………………………………………………………………………………………………………………….......... Translation Define translation ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... Describe the function of the genetic code (including degeneracy) ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... Identifying the key components of the process of translation M ……………………………………………………………………………………………… R ……………………………………………………………………………………………… C ……………………………………………………………………………………………… A ……………………………………………………………………………………………… T ……………………………………………………………………………………………… A ……………………………………………………………………………………………… P ……………………………………………………………………………………………… P ……………………………………………………………………………………………… Hint: Mr Cat App Summarise the process of translation ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... Use the genetic code to convert the following DNA sequence into a polypeptide sequence TAC AAA TTC GTA CTG CAC TCC GGA ACA ACT RNA: …………………………………………………………………. Protein: …………………………………………………………….. Outline the effect of the following mutations: GTA → GTT: ……………………………………………………… TCC → TCT: ……………………………………………………… Describe the different types of point mutations ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... Outline the significance of a frameshift mutation ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... Use the following image to explain how the universality of the genetic code allows for gene transfer Insulin gene Human Cell Recombinant Plasmid Bacteria Transgenic bacteria Grow in culture Extract insulin Plasmid ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... ………………………………………………………………………………………………………………………………………………….......... …………………………………………………………………………………………………………………………………………………..........
