HAPP 111 LB
HUMAN ANATOMY & PHYSIOLOGY
WITH PATHOSIOLOGY (Lab)
I.
1.
Basic Parts of Microscope
Eyepiece: It is the lens that is used to
study the samples and is placed at the
top. It has a magnification of 10X to 15X.
2.
Base: This provides support to the
microscope.
3.
Tube: This is used to connect the
eyepiece to the objective lenses.
4.
Objective lenses: These are found with
the magnification. The lower power lenses
are the shortest lens and the highest
power lenses are the longest lens.
Scanner – 4x (Red bond)
Low Power Objective – 10x
(Yellow Bond),
High Power Objective – 40x
(Blue bond)
Oil Immersion Objective –
100x (white bond)
Revolving nose-piece: This is also
known as the turret. It is used for holding
of other objective lens and can be rotated
while viewing the samples.
5.
6.
Diaphragm: It is used to control the
amount of light that passes through the
stage.
7.
Stage: It is the platform used for placing
the slides with samples.
8.
Stage clip: These are used to hold the
slides in the proper place.
9.
Coarse adjustment knob: It is used to
focus on scanning.
II.
Caring for the Microscope
Caring microscope with both hands – arm and base.
Don’t run when carrying microscope.
Check the bench/table if it clean and dry. Place in
center of clean, dry bench.
4. Treat microscope gently, don’t touch the lenses
5. Low to high rule – start with the lowest power
objective lens
6. Look from the side and turn coarse to raise stage.
7. Look through the eyepiece and slowly turn coarse
focus knob in opposite direction.
8. Remove slide, return to the lowest power lens
9. Clean and dry stage.
10. Store or dispose of glass slides responsibly.
1.
2.
3.
10. Fine adjustment knob: It is used to focus
on oil.
11. Arm: It is used to support the tube and
connects to the base of the microscope.
12. Power switch: The main power switch
used to turn on or off the microscope.
13. Condenser: It is used to focus the light on
the sample and 400X power lenses are
used.
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