Fast. Reliable. Close to patients. Analyticon Biotechnologies GmbH – Germany IUCell001_en_30_001_03.01_20220829 agile - affordable - accurate Urilyzer® Cell agile - affordable - accurate Agenda 1 Specifications 2 Setup 3 Software 4 Workflow 5 Maintenance agile - affordable - accurate Specifications Throughput Database Volume Up to 60 samples/hour 15 images/sample Storage of 10.000 measurements with 15 images each Only 175 µl per measurement Low space requirement Connectivity 2x Ethernet, 2x USB 3.0, 2x USB 3.1, 1x RS232 Specified Serial interfaces for Barcode Reader, Chemical Analyzer and LIS Compatibility Easy connection to urine test strip analyzer Urilyzer® 100 Pro / 500 Pro Whole Urinalysis result report on one device Consumables Urilyzer® Cell cuvettes (closed system, disposable) Pipetting tips No reagents needed agile - affordable - accurate Delivery contents • Urilyzer® Cell • Monitor • Keyboard • Mouse agile - affordable - accurate Setup Load the cuvettes Connect cabling Start the device agile - affordable - accurate Software Lets switch to the device agile - affordable - accurate Connection to Chemical Analyzer agile - affordable - accurate Connection to Chemical Analyzer The Urilyzer® Cell can be easily connected to the Urilyzer 100/500 Pro Backside of the Urilyzer® Cell (CHEM) via serial cable to chemical analyzer Urilyzer® 100 Pro: Go into Main → Options → Settings → Output → LIS2 → set Baudrate Urilyzer® 500 Pro: Go into Main → Settings → Output → LIS2 → set Baudrate Urilyzer® Cell: Go into Settings → Chemical Analyzer → choose „serial“ and the baudrate → Activate The chemical analyser has to be measured before the Urilyzer® Cell Use the same ID on both devices for each sample • Best with barcode reader (2 required) agile - affordable - accurate Workflow – Manual procedure Pressing the Start button to load an empty cuvette to pipetting position Barcode scanning Sample mixing Aspirating urine sample using manual pipette Injecting urine sample into cuvette Pressing the Start button to start the measurem ent agile - affordable - accurate Workflow – Automated procedure Centrifuging process Cuvette is placed into first microscope position Focusing process Cuvette is moved to next microscope position HPF-like image is taken by built-in camera NO Last image? Recorded image is evaluated YES Used cuvette is placed into the waste bin Results are forwarded to Data Manager Software agile - affordable - accurate Pipetting practice agile - affordable - accurate Pipetting practice agile - affordable - accurate Workflow – Manual procedure Pressing the Start button: to load an empty cuvette to pipetting position Barcode scanning Sample mixing Aspirating urine sample using manual pipette Injecting urine sample into cuvette Pressing the Start button: to start the measurement • Press the Start-Button on top of the device agile - affordable - accurate Workflow – Manual procedure Pressing the Start button: to load an empty cuvette to pipetting position Barcode scanning Sample mixing Aspirating urine sample using manual pipette Injecting urine sample into cuvette Pressing the Start button: to start the measurement • Press the Start-Button on top of the device • Enter ID - either manual or by a barcode scanner agile - affordable - accurate Workflow – Manual procedure Pressing the Start button: to load an empty cuvette to pipetting position Barcode scanning Sample mixing Aspirating urine sample using manual pipette Injecting urine sample into cuvette Pressing the Start button: to start the measurement • Press the Start-Button on top of the device • Enter ID - either manual or by a barcode scanner • Invert the sample several times – do not shake! agile - affordable - accurate Workflow – Manual procedure Pressing the Start button: to load an empty cuvette to pipetting position Barcode scanning Sample mixing Aspirating urine sample using manual pipette Injecting urine sample into cuvette Pressing the Start button: to start the measurement • Press the Start-Button on top of the device • Enter ID - either manual or by a barcode scanner • Invert the sample several times – do not shake! • Pick a pipette with 175 µl sample volume and aspirate the sample • Outer needle diameter of 1,2 – 2,8 mm agile - affordable - accurate Workflow – Manual procedure Pressing the Start button: to load an empty cuvette to pipetting position Barcode scanning Sample mixing Aspirating urine sample using manual pipette Injecting urine sample into cuvette Pressing the Start button: to start the measurement • Press the Start-Button on top of the device • Enter ID - either manual or by a barcode scanner • Invert the sample several times – do not shake! • Pick a pipette with 175 µl sample volume and aspirate the sample • Outer needle diameter of 1,2 – 2,8 mm • Fill the sample into the cuvette agile - affordable - accurate Workflow – Manual procedure Pressing the Start button: to load an empty cuvette to pipetting position Barcode scanning Sample mixing Aspirating urine sample using manual pipette Injecting urine sample into cuvette Pressing the Start button: to start the measurement • Press the Start-Button on top of the device • Enter ID - either manual or by a barcode scanner • Invert the sample several times – do not shake! • Pick a pipette with 175 µl sample volume and aspirate the sample • Outer needle diameter of 1,2 – 2,8 mm • Fill the sample into the cuvette • Press the Start-Button again to start the measurement process agile - affordable - accurate Workflow – Automated procedure Centrifuging process • Creating a monolayer of particles by centrifuging the cuvette Cuvette is placed into first microscope position Focusing process Cuvette is moved to next microscope position 10 seconds with 2000 RPM HPF-like image is taken by built-in camera NO Last image? Recorded image is evaluated YES Used cuvette is placed into the waste bin Results are forwarded to Data Manager Software agile - affordable - accurate Workflow – Automated procedure Centrifuging process • Creating a monolayer of particles by centrifuging the cuvette • Cuvette is moved above microscope Cuvette is placed into first microscope position Focusing process Cuvette is moved to next microscope position HPF-like image is taken by built-in camera NO Last image? Recorded image is evaluated YES Used cuvette is placed into the waste bin Results are forwarded to Data Manager Software agile - affordable - accurate Workflow – Automated procedure Centrifuging process • Creating a monolayer of particles by centrifuging the cuvette • Cuvette is moved above microscope Cuvette is placed into first microscope position • Focus series is captured in different heights Focusing process Cuvette is moved to next microscope position HPF-like image is taken by built-in camera NO Last image? Recorded image is evaluated YES Used cuvette is placed into the waste bin Results are forwarded to Data Manager Software agile - affordable - accurate Workflow – Automated procedure Centrifuging process • Creating a monolayer of particles by centrifuging the cuvette • Cuvette is moved above microscope Cuvette is placed into first microscope position • Focus series is captured in different heights • Camera takes a picture of the sharpest plane Focusing process Cuvette is moved to next microscope position HPF-like image is taken by built-in camera NO Last image? Recorded image is evaluated YES Used cuvette is placed into the waste bin Results are forwarded to Data Manager Software agile - affordable - accurate Workflow – Automated procedure Centrifuging process • Creating a monolayer of particles by centrifuging the cuvette • Cuvette is moved above microscope Cuvette is placed into first microscope position • Focus series is captured in different heights • Camera takes a picture of the sharpest plane Focusing process Cuvette is moved to next microscope position • The system checks if this was the last picture HPF-like image is taken by built-in camera NO Last image? • The image gets automatically evaluated and is saved in the database Recorded image is evaluated YES Used cuvette is placed into the waste bin Results are forwarded to Data Manager Software agile - affordable - accurate Workflow – Automated procedure Centrifuging process Cuvette is placed into first microscope position Focusing process Cuvette is moved to next microscope position • The system checks if this was the last picture HPF-like image is taken by built-in camera NO Last image? Recorded image is evaluated YES Used cuvette is placed into the waste bin • YES: The cuvette is moved into the waste bin • NO: the cuvette position moves to next microscope position Results are forwarded to Data Manager Software agile - affordable - accurate Results agile - affordable - accurate Auto classification • Red blood cells • White blood cells • • White blood cell clumps Crystals • Calcium-oxalate monohydrate • Calcium-oxalate dihydrate • Triple phosphate • Uric acid • Hyaline casts • Pathological casts • Non-squamous epithelial cells • Squamous epithelial cells • Yeast • Bacteria • Bacteria rods • Bacteria cocci • Mucus • Spermatozoa agile - affordable - accurate Auto classification Particle type: Subclasses and other particles can be added Semi-quantitative category: The ranges and category name can be changed Quantitative results: p/µl or p/HPF Negative result limits RBC and WBC are real quantitative results Every other result is semi-quantitative even if the result is displayed quantitative agile - affordable - accurate Calculation • π»ππΉ − ππππ ∗ 1,5 = π»ππΉ • πΏππΉ − ππππ ∗ 0,5 = π»ππΉ HPF-like Semi-quantitative parameter HPF • π • π ΰ΅µπ = Τπ»ππΉ = σπ 15 ∗ 6,6 σπ 15 ∗ 1,5 LPF-like LPF RBC and WBC calculation • RBC: πΤπ»ππΉ ∗ 2 • WBC: πΤπ»ππΉ ∗ 1,5 Urilyzer® Cell p/HPF is correlated with 20x concentrated manual measurement. agile - affordable - accurate Focussing process • 2000 steps ≡ 2,5 mm • Hard Focus Start ~ 800 • 1 step ≡ 1,25 µm • Set with the Reference Cuvette in Service Software Taking 1 image every two steps → chooses the best Best image heigth of the previous one defines the start → taking 16 images in total and choses the best again 2000 steps } 320 steps 800 Pic. 1 Best image heigth of the previous one defines the start → taking 16 images in total and choses the best again } 16 steps up & 16 steps down Pic. 2 } 16 steps up & 16 steps down Pic. 3 And so on with the remaining 12 images agile - affordable - accurate Manual addable sediments • Up to 50 parameters are manual addable • The red ones are only manual addable • Can be used for further information • Parameters must be activated to add them agile - affordable - accurate Review criteria 0-2 review images → normal measurement: The results are calculated based on the non-review images → can be auto-validated ≥ 3 review images and ≥ 5 O.K. images: review measurement: The results are calculated based on the non-review images → cannot be auto-validated < 5 O.K. images: review measurement: No automated result → cannot be auto-validated User can modify the final sample results based on the manual review of the images or remeasure the sample with a dilution MUC review: Concentration limit can be set for MUC: if the sample concentration is above this limit, the measurement will be flagged for review The results are calculated based on the images → cannot be autovalidated agile - affordable - accurate Maintenance • Daily routine should maintain a cleaning process after every workshift, 24 hours or according to the local regulations • What should be cleaned: • • • • • • Cuvette feeder arm Cuvette hold down assembly Drop tray Centrifuge cover Centrifuge arm The path of the cuvette, especially the top of the microscope • Waste bin • The outer surface, especially the pipetting window agile - affordable - accurate We look forward to the opportunity to work with you. agile - affordable - accurate
