Review Concepts
DNA polymerase
Uracil vs thymine
Methods of labelling DNA (2)
RNA world hypothesis (2)
Sanger sequencing
Types of RNA (9)
-
ddNTPs
Automated vs manual
Pros (3) and cons (4)
Shotgun sequencing (3)
-
Assembly (consensus sequence +
coverage)
Human genome project
-
BACs
Next generation sequencing – Illumina
-
Pros (3) and cons (5)
Reversible
Prokaryotes
-
Eukaryotes
-
Ion torrent
-
Pros (5) and cons (3)
Third-generation sequencing (3)
Microarray
RNA sequencing
-
Transcriptome
(sn)RNAseq
tSNE mapping
Velocity experiments
ChIP-seq
Ribosome profiling
RNA polymerase (steps + abortive
initiative)
Location
Polycistronic
-
RNA polymerase I, II, III
Mediators
PREP steps + additional ingredients
Locations
5’ cap (I/II/III?, what is it?, end
linkage, purpose (4))
RNA splicing (steps, mediator,
sequences)
PolyA tail (purpose, components,
steps)
Translation
-
tRNA
Wobble position
Steps that AA gets added to tRNA
Polypeptide chain
-
Steps and components
Ribosome
-
Eukaryotes vs prokaryotes
Structure
Steps