Observing patterns by ecological sampling Aim: To carry out a study on the ecology of a habitat, by using quadrats and transects to determine distribution and abundance of organisms, measuring abiotic factors. Hypothesis: As light intensity increases, The abundance of organisms increases. Equipment: Quadrat Transect Ph/Moisture meter Method: 1. Choose an area where there is a clear gradient in the abiotic variable and place the transect down. Select a species that changes in abundance along the gradient. 2. Place the quadrat at each of the marks on the transect, placing the bottom left corner on the mark every time. 3. Record the percentage cover for the chosen species. This can be done by recording how many of the quadrat’s 100 squares contain the chosen species. A square should only be counted if half or more of it is covered. 4. At each coordinate, a measure of the independent variable should be taken. Using a pH and light meter, light intensity and pH of soil can be measured at each different location. Position on Transect (M) 1 2 3 4 5 6 7 8 9 10 8 8 8 pH Moisture Daisies 8 8 7 8 8 8 8 4.5 1.5 4.5 1.5 3 5 4.5 0 2 0 4 0 2 11 4 5 2.5 1 0 0 Conclusion