BIOL224 Assignment #5: Microbial control– vpm034 (200443517) - W 2:30 (L02) By recording my initials here [VP], I declare that the following assignment is my own original, unaided work. Furthermore, I declare that I have cited and referenced the ideas of others; I understand the penalties for failing to do so. 1. The main objective of this lab is how microbial colonization occurs and based on this we can create a hypothesis, using diffusion disc experiments and studying how microorganisms travel, we will be able to help restrict the spread of an infection in this way. We can identify how the infection has become antibiotic-resistant and what other drugs should be supplied using a pus sample from the patient. We'll also learn about different sterilisation and decontamination methods, as well as how to avoid illness spread. Because the patient has an infection that appears to be resistant to some antibiotics, we will be able to discover which antimicrobial compounds are successful if we can do a diffusion disc assay, and maybe this will cure the infection. 2. F 3. A. Collect the sample of the patient the purpose is to determine the drug which can prevent the infection. B. Use a detergent and the autoclave to disinfect Doctor’s dental tools to control microbial growth. C. Place samples on a blood agar plate and compare the two samples from 2019 to the patient. D. Chemically doped filter discs are used in a diffusion disc assay to see how susceptible bacteria are to penicillin. E. Using the holo table to determine an antibiotic's effect on a bacterium and which antibiotic would be most effective in eliminating it 4. We can utilise a diffusion disc experiment to discover which drugs would be effective in stopping or reducing an infection by understanding how to regulate microbial development. 5.